Biosensing by dynamic magnetization measurement of stimuli-responsive magnetic hydrogels

• Project/Area Number: 18H01824
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28030:Nanomaterials-related
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Kitamoto Yoshitaka 東京工業大学, 物質理工学院, 教授 (10272676)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: バイオセンシング / ハイドロゲル / 刺激応答性高分子 / 磁性ナノ粒子 / 交流磁化計測 / 磁気微粒子 / 磁化計測

Outline of Final Research Achievements

Ferrogels composed of magnetic nanoparticles and stimuli-responsive hydrogels have been synthesized as a magnetic label for chemical/biochemical biosensors. Magnetic nanoparticles are dispersed in a stimuli-responsive polymer hydrogel with keeping a capability of physical motions such as rotation and vibration, exhibiting a magnetic relaxation (Brownian relaxation) based on their hydrodynamic behaviors against alternating magnetic fields. Modulations of the hydrodynamic behaviors of magnetic nanoparticles in the ferrogels have been detected by dynamic magnetization measurements of the ferrogels with a swelling behavior of a pH-responsive polymer hydrogel. The detection of ammonia gas has been successfully demonstrated using the pH-responsive ferrogel from the dynamic magnetization measurements.

Academic Significance and Societal Importance of the Research Achievements

生体から取り出した体液やガスなどを計測することで、健康や疾患の状態を評価する技術は予防医学や迅速な診断のために不可欠の医療技術であり、本研究はそれを実現するのに遠隔操作も可能な磁気技術を活用し、簡便かつ高精度のセンシング技術に資するものである。そのための材料開発において、化学的な操作によって磁性材料の特性を変化させる、つまり磁気学と化学・生化学を融合するという新しい視点を導入し、その成果として皮膚ガスの成分でもあるアンモニアガスを磁気ハイドロゲルと磁気センサを使って計測し、健康や医療の診断に資するデータを提供しうる可能性があることを実験的に示すことに成功した。

Research Products

• [Int'l Joint Research] University College London(英国)
• [Int'l Joint Research] University College London(英国)
• [Int'l Joint Research] University College London(英国)
• [Journal Article] Effect of Ionic Concentration on Dynamic Magnetic Susceptibility of Iron Oxide Nanoparticles Embedded in Chitosan Hydrogel Matrix (2019)
  • Author(s): M. E. Villamin, Y. Kitamoto
  • Journal Title: Transaction of the Magnetics Society of Japan Special Issues Volume: 3 Issue: 1 Pages: 113-119
  • DOI: 10.20819/msjtmsj.19TR322
  • NAID: 130007641698
  • ISSN: 2432-0471
  • Year and Date: 2019-05-01
  • Peer Reviewed
• [Journal Article] Distributive Activation Volumes of Magnetically Interacting Nanostructures (2019)
  • Author(s): Trisnanto Suko Bagus、Kitamoto Yoshitaka
  • Journal Title: The Journal of Physical Chemistry C Volume: 123 Issue: 38 Pages: 23732-23737
  • DOI: 10.1021/acs.jpcc.9b05584
  • Peer Reviewed
• [Journal Article] Fabrication of porous FePt microcapsules for immunosensing techniques (2019)
  • Author(s): Kumiko Miyajima, Yuki Miwa, Yoshitaka Kitamoto
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 173 Pages: 407-411
  • DOI: 10.1016/j.colsurfb.2018.10.004
  • Peer Reviewed
• [Journal Article] Influence of pH on Dynamic Magnetic Susceptibility of Iron-Oxide Nanoparticles in a Chitosan Hydrogel Matrix (2019)
  • Author(s): Villamin Maria Emma、Kitamoto Yoshitaka
  • Journal Title: IEEE Transactions on Magnetics Volume: 55 Issue: 2 Pages: 1-4
  • DOI: 10.1109/tmag.2018.2869828
  • Peer Reviewed
• [Journal Article] Synthesis of multifunctional clustered nano-Fe3O4 chitosan nanocomposite for biomedical applications (2018)
  • Author(s): Villamin Maria Emma、Kitamoto Yoshitaka
  • Journal Title: AIP Conference Proceedings Volume: 1929 Pages: 020014-020014
  • DOI: 10.1063/1.5021927
  • Peer Reviewed
• [Journal Article] Fabrication of Fe3O4 nanorods designed for liquid-phase magnetic biosensing (2018)
  • Author(s): Yasuda Kazuki、Kitamoto Yoshitaka
  • Journal Title: AIP Conference Proceedings Volume: 1929 Pages: 020009-020009
  • DOI: 10.1063/1.5021922
  • Peer Reviewed
• [Journal Article] Influence of behaviors of magnetic particles in ferrofluids under alternating magnetic fields on harmonic responses (2018)
  • Author(s): Oda Shoya、Trisnanto Suko Bagus、Kitamoto Yoshitaka
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Issue: 2S2 Pages: 02CB17-02CB17
  • DOI: 10.7567/jjap.57.02cb17
  • Peer Reviewed
• [Journal Article] Dipolar magnetism and electrostatic repulsion of colloidal interacting nanoparticle system (2018)
  • Author(s): Trisnanto Suko Bagus、Yasuda Kazuki、Kitamoto Yoshitaka
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Issue: 2S2 Pages: 02CC06-02CC06
  • DOI: 10.7567/jjap.57.02cc06
  • Peer Reviewed
• [Presentation] 銅イオンとの相互作用によるフミン酸修飾酸化鉄磁性ナノ粒子の交流磁化特性への影響 (2020)
  • Author(s): 原田 有紀、北本 仁孝
  • Organizer: 粉体粉末冶金協会2020年度秋季大会
• [Presentation] 遠心分離を用いたキトサンハイドロゲルビーズの生成条件の検討 (2020)
  • Author(s): 倉科佑太、竹原万莉那、Ryo Torii、尾上弘晃、北本仁孝
  • Organizer: 化学とマイクロ・ナノシステム学会 第42回研究会
• [Presentation] Optimized coil-based complex magnetic susceptibility measurement system for higher sensitivity and wider frequency range (2020)
  • Author(s): S. Yang, Y. Kurashina, Y. Kitamoto
  • Organizer: Virtual Irago Conference 2020
• [Presentation] Temperature Responsive Core-Shell Nanocomposites Consisting of Catalytic Co-polymer and Iron-oxide Nanoparticles as Phase Transfer Catalyst (2020)
  • Author(s): R. Huang, Y. Kurashina, Y. Kitamoto
  • Organizer: Virtual Irago Conference 2020
• [Presentation] Magnetic Bio-sensing Using the Distribution of Hydrodynamic Diameter Calculated from AC Magnetization (2020)
  • Author(s): Ryota Sasaki, Yuta Kurashina, Yoshitaka Kitamoto
  • Organizer: Virtual Irago Conference 2020
• [Presentation] Dynamic Magnetization Measurements of Ferrofluids using Giant Magnetoresistive Sensors (2020)
  • Author(s): Y.Takahashi,Y. Kurashina, Y. Kitamoto
  • Organizer: Virtual Irago Conference 2020
• [Presentation] Effect of pH on dynamic magnetization behavior of magnetic nanoparticles in polymer aqueous solution (2020)
  • Author(s): M. Takehara, Y. kurashina, Y. Kitamoto
  • Organizer: Virtual Irago Conference 2020
• [Presentation] Synthesis of Iron-Oxide Nanoparticles/Thermoresponsive PolymerComposite Hydrogels with Controlled Porous Structure (2019)
  • Author(s): Ke Xu, Ye Chen, Yuta Kurashina, Yoshitaka Kitamoto
  • Organizer: EM-NANO2019
  • Int'l Joint Research
• [Presentation] Biomedical Applications of Magnetic Nanoparticles (2019)
  • Author(s): Yoshitaka Kitamoto
  • Organizer: 4th International Symposium on Current Progress in Functional Materials 2019
  • Int'l Joint Research / Invited
• [Presentation] pH sensing based on AC magnetization of magnetic nanoparticles contained in hydrogel microbeads (2019)
  • Author(s): Yuta Kurashina, Maria Emma C. Villamin, Marina Takehara, Yoshitaka Kitamoto
  • Organizer: 12th International Conference on Ferrites
  • Int'l Joint Research
• [Presentation] Heating efficiency and dipolar interaction in magnetic nanoparticle ensembles (2019)
  • Author(s): Chiemi Oka, Nanao Horiishi, Yoshitaka Kitamoto
  • Organizer: 12th International Conference on Ferrites
  • Int'l Joint Research
• [Presentation] コロイド中での酸化鉄ナノ粒子の分散性と交流磁化 特性に関する研究 (2019)
  • Author(s): 生田 歩夢, 佐々木 瞭太, 北本 仁孝
  • Organizer: 粉体粉末冶金協会2019年度春季大会
• [Presentation] 酸化鉄ナノ粒子/熱応答性高分子複合ハイドロゲル の多孔質構造制御 (2019)
  • Author(s): 徐 珂, 陳 曄, 倉科 佑太, 北本 仁孝
  • Organizer: 粉体粉末冶金協会2019年度春季大会
• [Presentation] 磁性ナノ粒子とキトサンハイドロゲルからなる磁性複合ゲルの作製 (2019)
  • Author(s): 竹原万莉那, ヴィリャミン マリア エマ, 倉科佑太, 北本仁孝
  • Organizer: 粉体粉末冶金協会2019年度秋季大会
• [Presentation] 流動性媒体中の磁性ナノ粒子の交流磁場応答とそのバイオデバイスへの応用 (2019)
  • Author(s): 北本 仁孝，小田 翔也，生田 歩夢， Maria Emma Villamin
  • Organizer: 平成31年電気学会全国大会
• [Presentation] Study on composites of magnetic nanoparticles and pH-responsive hydrogel for magnetic sensing labels (2019)
  • Author(s): M. Abe, Y. Kurashina, Y. Kitamoto
  • Organizer: IRAGO Conference 2019
  • Int'l Joint Research
• [Presentation] Synthesis of Iron-Oxide Nanoparticles/PNIPAM Hydrogel Composites with Controlled Porous Structure based on Phase Separation (2019)
  • Author(s): Ke Xu, Ye Chen, Yuta Kurashina, Yoshitaka Kitamoto
  • Organizer: IRAGO Conference 2019
  • Int'l Joint Research
• [Presentation] Study on Relationship between Properties of Water in Hydrogel and AC Magnetization of Magnetic Nanoparticles (2019)
  • Author(s): X.Zhao, Y.Kurashina, Y. Kitamoto
  • Organizer: IRAGO Conference 2019
  • Int'l Joint Research
• [Presentation] ソルボサーマル合成における酸化鉄磁性ナノ粒子の粒径制御 (2018)
  • Author(s): 佐々木瞭太, 生田歩夢, 倉科佑太, 北本仁孝
  • Organizer: 粉体粉末冶金協会 平成30年秋季大会
• [Presentation] 液中で生体分子を補足した酸化鉄ナノ粒子の交流磁界に対する振舞の解析 (2018)
  • Author(s): 生田 歩夢, 北本 仁孝
  • Organizer: 2018年電気化学秋季大会
• [Presentation] Eect of ionic concentration on dynamic magnetic susceptibility of iron oxide nanoparticles embedded in chitosan hydrogel matrix (2018)
  • Author(s): M. E. Villamin, Y. Kitamoto
  • Organizer: 第42回日本磁気学会学術講演会
• [Presentation] 液中における酸化鉄ナノ粒子の交流磁界に対する振舞の解析 (2018)
  • Author(s): 生田 歩夢, 北本 仁孝
  • Organizer: 第42回日本磁気学会学術講演会
• [Book] Nanomagnetic Materials: Fabrication, Characterization and Application (2021)
  • Author(s): Akinobu Yamaguchi Atsufumi Hirohata, Yoshitaka Kitamoto, et.al.
  • Total Pages: 812
  • Publisher: Elsevier
• [Remarks] 北本研究室ホームページ
  • URL: http://www.kitamoto.iem.titech.ac.jp/