Synthesis and Virus Capture of Self-Propelled Protein Microtube Motors by Bubble Jetting of Enzyme Reaction
| Project/Area Number |
18H01833
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 28030:Nanomaterials-related
|
|---|
| Research Institution | Chuo University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
森田 能次 中央大学, 理工学部, 助教 (40795308)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
|---|
| Keywords | タンパク質 / カタラーゼ / ウレアーゼ / 大腸菌 / ウイルス / 酵素 / 金ナノ粒子 / マイクロチューブ / 交互積層 / ヘマグルチニン / アビジンービオチン / 自己攪拌能 / アビジン-ビオチン / 酵素活性 / 自走能 |
|---|
| Outline of Final Research Achievements |
We have synthesized microtube (MT) motors with a cylindrical hollow structure consisting of proteins. The MT with catalase on the inner surface was self-propelled by jetting oxygen bubbles generated by the disproportionation of hydrogen peroxide. The MT with urease interior surface was self-propelled by ejection of the product generated by the decomposition of urea. Furthermore, the MT motors coated with concanavalin A captured Escherichia coli. The MT motors coated with α-glucosidase acted as a self-stirring catalyst. The MT motor coated with fetuin captured efficiently the spike protein of influenza virus. In addition, the self-propelling velocity of MT coated with gold nanoparticles increased by light irradiation.
|
| Academic Significance and Societal Importance of the Research Achievements |
酵素反応を駆動力源として自走する蛋白質MTモーターの一群を合成し、それらの機能を明らかにした。得られた成果は、生体適合性に優れた薬物キャリアー、バイオコンテナー、病原体クリーナー、バイオ分子マシンなど、様々な新規生体機能材料の創出につながると考えられる。薬物送達システムの分子設計に新たな指針を与えるばかりでなく、バイオマテリアル化学に新領域を拓くものと期待される。人類の健康・福祉にもたらす波及効果と意義もきわめて大きい。
|
Report
(4 results)
Research Products
(34 results)
