High-speed AFM study on functional modulation of kinesin caused by structural defects of mictoruble
Project/Area Number |
18H01837
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 28040:Nanobioscience-related
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Research Institution | Nagoya University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
Keywords | 一分子計測 / 微小管 / キネシン / タンパク質 / 原子間力顕微鏡 / 高速原子間力顕微鏡 / 力学特性マッピング / モータータンパク質 / 高速AFM |
Outline of Final Research Achievements |
In this study, we have developed an in-line force-curve mode, which is an advanced molecular manipulation function of high-speed atomic force microscopy (AFM), allowing us to form single tubulin dimer defects in microtubules with high controllability and to obtain force-distance curves in realtime. This technique enabled us to observe the self-healing process of structural defects created on a microtubule and the translational motility of kinesin molecules around the defects, and to quantify the binding energy between tubulins. Furthermore, we have extended this technique to the fast force mapping mode. Also, by observing the kinesin sliding motion in bent microtubules, we found that the kinesin translational velocity is significantly reduced in the bent region.
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Academic Significance and Societal Importance of the Research Achievements |
本研究で開発された手法は、微小管の機械特性によるMAPsの機能変調を解析できる唯一手法であり、今後微小管に限らず様々なタンパク質の一分子構造操作や機能解析が可能になることから、生物学の重要な計測ツールとなり学術的意義は高い。
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Report
(4 results)
Research Products
(84 results)
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[Journal Article] Structural insights into the mechanism of rhodopsin phosphodiesterase2020
Author(s)
Ikuta T, Shihoya W, Sugiura M, Yoshida K, Watari M, Tokano T, Yamashita K, Katayama K, Tsunoda SP, Uchihashi T, Kandori H and Nureki O.
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Journal Title
Nature Communications_
Volume: 11
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Convergent evolution of processivity in bacterial and fungal cellulases2020
Author(s)
Uchiyama, T., Uchihashi, T., Nakamura, A., Watanabe, H., Kaneko, S., Samejima, M., and Igarashi K.
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 117
Pages: 19896-19903
DOI
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Peer Reviewed / Open Access
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[Journal Article] Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species.2020
Author(s)
Terashima H, Hirano K, Inoue Y, Tokano T, Kawamoto A, Kato T, Yamaguchi E, Namba K, Uchihashi T, Kojima S, Homma M.
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Journal Title
Journal of Bacteriology.
Volume: -
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Peer Reviewed
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[Journal Article] Crystal structure of heliorhodopsin2020
Author(s)
W. Shihoya, K. Inoue, M. Singh, M. Konno, S. Hososhima, K. Yamashita, K. Ikeda, A. Higuchi, T. Izume, Okazaki S, Hashimoto M, Mizutori R, Tomida S, Yamauchi Y, Abe-Yoshizumi R, K. Katayama, SP. Tsunoda, M. Shibata, Y. Furutani, A. Pushkarev, O. Béjà, T. Uchihashi, H. Kandori, O. Nureki
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Journal Title
Nature
Volume: 574
Pages: 132-136
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q.2020
Author(s)
Saeko Yanaka,Rina Yogo,Hiroki Watanabe,Yuki Taniguchi,Tadashi Satoh,Naoko Komura,Hiromune Ando,Hirokazu Yagi,Nobuhiro Yuki,Takayuki Uchihashi,Koichi Kato
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Journal Title
International Journal of Molecular Sciences
Volume: 21
Pages: 147-147
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation2020
Author(s)
Yagi-Utsumi M., Sikdar A., Song C., Park J., Inoue R., Watanabe H., Burton-Smith R. N., Kozai T., Suzuki T., Kodama A., Ishii K., Yagi H., Satoh T., Uchiyama S., Uchihashi T., Joo K., Lee J., Sugiyama M., Murata K., Kato K.
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Journal Title
Scientific Reports
Volume: 10
Pages: 1-10
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mutational and Combinatorial Control of Self-Assembling and Disassembling of Human Proteasome alpha Subunits2019
Author(s)
Sekiguchi T., Satoh T., Kurimoto E., Song C., Kozai T., Watanabe H., Ishii K., Yagi H., Yanaka S., Uchiyama S., Uchihashi T., Murata K., Kato K.
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Journal Title
International Journal of Molecular Sciences
Volume: 20
Pages: 2308-2308
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III.2019
Author(s)
[Rina Yogo,Yuki Yamaguchi,Hiroki Watanabe,Hirokazu Yagi,Tadashi Satoh,Mahito Nakanishi,Masayoshi Onitsuka,Takeshi Omasa,Mari Shimada,Takahiro Maruno,Tetsuo Torisu,Shio Watanabe,Daisuke Higo,Takayuki Uchihashi,Saeko Yanaka,Susumu Uchiyama,Koichi Kato]
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Journal Title
Scientific Reports
Volume: 9
Pages: 1-10
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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