High-speed AFM study on functional modulation of kinesin caused by structural defects of mictoruble

• Project/Area Number: 18H01837
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28040:Nanobioscience-related
• Research Institution: Nagoya University
• Principal Investigator: Uchihashi Takayuki 名古屋大学, 理学研究科, 教授 (30326300)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 一分子計測 / 微小管 / キネシン / タンパク質 / 原子間力顕微鏡 / 高速原子間力顕微鏡 / 力学特性マッピング / モータータンパク質 / 高速AFM

Outline of Final Research Achievements

In this study, we have developed an in-line force-curve mode, which is an advanced molecular manipulation function of high-speed atomic force microscopy (AFM), allowing us to form single tubulin dimer defects in microtubules with high controllability and to obtain force-distance curves in realtime. This technique enabled us to observe the self-healing process of structural defects created on a microtubule and the translational motility of kinesin molecules around the defects, and to quantify the binding energy between tubulins. Furthermore, we have extended this technique to the fast force mapping mode. Also, by observing the kinesin sliding motion in bent microtubules, we found that the kinesin translational velocity is significantly reduced in the bent region.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発された手法は、微小管の機械特性によるMAPsの機能変調を解析できる唯一手法であり、今後微小管に限らず様々なタンパク質の一分子構造操作や機能解析が可能になることから、生物学の重要な計測ツールとなり学術的意義は高い。

Research Products

• [Journal Article] Non-close-packed arrangement of soft elastomer microspheres on solid substrates (2021)
  • Author(s): Yuma Sasaki, Seina Hiroshige, Masaya Takizawa, Yuichiro Nishizawa, Takayuki Uchihashi, Haruka Minato and Daisuke Suzuki
  • Journal Title: RSC Advances Volume: 11 Pages: 14562-14567
  • DOI: 10.1039/d1ra02688g
  • Peer Reviewed / Open Access
• [Journal Article] Nanostructure and thermoresponsiveness of poly(N-isopropyl methacrylamide)-based hydrogel microspheres prepared via aqueous free radical precipitation polymerization (2021)
  • Author(s): Nishizawa Yuichiro、Minato Haruka、Inui Takumi、Saito Ikuma、Kureha Takuma、Shibayama Mitsuhiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: RSC Advances Volume: 11 Pages: 13130-13137
  • DOI: 10.1039/d1ra01650d
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy (2021)
  • Author(s): Fujita Junso、Sugiyama Shogo、Terakado Haruna、Miyazaki Maho、Ozawa Mayuki、Ueda Nanami、Kuroda Natsuko、Tanaka Shun-ichi、Yoshizawa Takuya、Uchihashi Takayuki、Matsumura Hiroyoshi
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Pages: 1697-1697
  • DOI: 10.3390/ijms22041697
  • Peer Reviewed / Open Access
• [Journal Article] Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution (2020)
  • Author(s): Maity Basudev、Li Zhipeng、Niwase Kento、Ganser Christian、Furuta Tadaomi、Uchihashi Takayuki、Lu Diannan、Ueno Takafumi
  • Journal Title: Physical Chemistry Chemical Physics Volume: 22 Pages: 18562-18572
  • DOI: 10.1039/d0cp02069a
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nanostructures, Thermoresponsiveness, and Assembly Mechanism of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization (2020)
  • Author(s): Nishizawa Yuichiro、Minato Haruka、Inui Takumi、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Langmuir Volume: 37 Pages: 151-159
  • DOI: 10.1021/acs.langmuir.0c02654
  • Peer Reviewed
• [Journal Article] Novel Babesia bovis exported proteins that modify properties of infected red blood cells (2020)
  • Author(s): Hakimi H, Templeton TJ, Sakaguchi M, Yamagishi J, Miyazaki S, Yahata K, Uchihashi T, Kawazu SI, Kaneko O, Asada M
  • Journal Title: PLoS pathogens Volume: 16(10)
  • DOI: 10.1371/journal.ppat.1008917
  • NAID: 120006988490
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural insights into the mechanism of rhodopsin phosphodiesterase (2020)
  • Author(s): Ikuta T, Shihoya W, Sugiura M, Yoshida K, Watari M, Tokano T, Yamashita K, Katayama K, Tsunoda SP, Uchihashi T, Kandori H and Nureki O.
  • Journal Title: Nature Communications_ Volume: 11 Pages: 1-10
  • DOI: 10.1038/s41467-020-19376-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Convergent evolution of processivity in bacterial and fungal cellulases (2020)
  • Author(s): Uchiyama, T., Uchihashi, T., Nakamura, A., Watanabe, H., Kaneko, S., Samejima, M., and Igarashi K.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 117 Pages: 19896-19903
  • DOI: 10.1073/pnas.2011366117
  • Peer Reviewed / Open Access
• [Journal Article] Direct visualization of the conformational change of FUS/TLS upon binding to promoter-associated non-coding RNA (2020)
  • Author(s): Nesreen Hamad, Hiroki Watanabe, Takayuki Uchihashi, Riki Kurokawa, Takashi Nagata, and Masato Katahira
  • Journal Title: Chemical Communications Volume: 56 Pages: 9134-9137
  • DOI: 10.1039/d0cc03776a
  • Peer Reviewed / Open Access
• [Journal Article] Structural Dynamics of a Protein Domain Relevant to the Water-Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy (2020)
  • Author(s): Tokano Takaya、Kato Yuki、Sugiyama Shogo、Uchihashi Takayuki、Noguchi Takumi
  • Journal Title: J. Phys. Chem. B Volume: 124 Pages: 5847-5857
  • DOI: 10.1021/acs.jpcb.0c03892
  • Peer Reviewed
• [Journal Article] Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species. (2020)
  • Author(s): Terashima H, Hirano K, Inoue Y, Tokano T, Kawamoto A, Kato T, Yamaguchi E, Namba K, Uchihashi T, Kojima S, Homma M.
  • Journal Title: Journal of Bacteriology. Volume: -
  • DOI: 10.1128/jb.00236-20
  • Peer Reviewed
• [Journal Article] Thermoresponsive structural changes of single poly(N-isopropyl acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate (2020)
  • Author(s): Minato Haruka、Nishizawa Yuichiro、Uchihashi Takayuki、Suzuki Daisuke
  • Journal Title: Polymer Journal Volume: 52 Pages: 1137-1141
  • DOI: 10.1038/s41428-020-0372-3
  • NAID: 40022335719
  • Peer Reviewed
• [Journal Article] Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy (2020)
  • Author(s): Ueno Takafumi、Niwase Kento、Tsubokawa Daisho、Kikuchi Kosuke、Takai Natsumi、Furuta Tadaomi、Kawano Ryuji、Uchihashi Takayuki
  • Journal Title: Nanoscale Volume: in press Pages: 8166-8173
  • DOI: 10.1039/d0nr01121e
  • Peer Reviewed
• [Journal Article] Recent advances in bioimaging with high-speed atomic force microscopy (2020)
  • Author(s): Uchihashi Takayuki、Ganser Christian
  • Journal Title: Biophysical reviews Volume: 12 Pages: 363-369
  • DOI: 10.1007/s12551-020-00670-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy (2020)
  • Author(s): Konno H, Watanabe-Nakayama T, Uchihashi T, Okuda M, Zhu L, Kodera N, Kikuchi Y, Ando T, Taguchi H
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Pages: 7831-7836
  • DOI: 10.1073/pnas.1916452117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Processes of Its Formation (2020)
  • Author(s): Oohora Koji、Hirayama Shota、Uchihashi Takayuki、Hayashi Takashi
  • Journal Title: Chem. Lett. Volume: 49 Issue: 2 Pages: 186-190
  • DOI: 10.1246/cl.190855
  • NAID: 130007795242
  • ISSN: 0366-7022, 1348-0715
  • Peer Reviewed
• [Journal Article] Crystal structure of heliorhodopsin (2020)
  • Author(s): W. Shihoya, K. Inoue, M. Singh, M. Konno, S. Hososhima, K. Yamashita, K. Ikeda, A. Higuchi, T. Izume, Okazaki S, Hashimoto M, Mizutori R, Tomida S, Yamauchi Y, Abe-Yoshizumi R, K. Katayama, SP. Tsunoda, M. Shibata, Y. Furutani, A. Pushkarev, O. Béjà, T. Uchihashi, H. Kandori, O. Nureki
  • Journal Title: Nature Volume: 574 Pages: 132-136
  • DOI: 10.1038/s41586-019-1604-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q. (2020)
  • Author(s): Saeko Yanaka,Rina Yogo,Hiroki Watanabe,Yuki Taniguchi,Tadashi Satoh,Naoko Komura,Hiromune Ando,Hirokazu Yagi,Nobuhiro Yuki,Takayuki Uchihashi,Koichi Kato
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Pages: 147-147
  • DOI: 10.3390/ijms21010147
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge (2020)
  • Author(s): Tatebe Hisashi、Lim Chew Theng、Konno Hiroki、Shiozaki Kazuhiro、Shinohara Akira、Uchihashi Takayuki、Furukohri Asako
  • Journal Title: Nature Communications Volume: 11 Pages: 370-370
  • DOI: 10.1038/s41467-019-14025-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System (2020)
  • Author(s): Hirayama Shota、Oohora Koji、Uchihashi Takayuki、Hayashi Takashi
  • Journal Title: Journal of American Chemical Society Volume: 142 Pages: 1822-1831
  • DOI: 10.1021/jacs.9b10080
  • Peer Reviewed / Open Access
• [Journal Article] Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation (2020)
  • Author(s): Yagi-Utsumi M., Sikdar A., Song C., Park J., Inoue R., Watanabe H., Burton-Smith R. N., Kozai T., Suzuki T., Kodama A., Ishii K., Yagi H., Satoh T., Uchiyama S., Uchihashi T., Joo K., Lee J., Sugiyama M., Murata K., Kato K.
  • Journal Title: Scientific Reports Volume: 10 Pages: 1-10
  • DOI: 10.1038/s41598-020-58371-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies (2020)
  • Author(s): Takayuki Umakoshi, Shingo Fukuda, Ryota Iino, Takayuki Uchihashi, Toshio Ando*
  • Journal Title: Biochimica et Biophysica acta. General subjects Volume: 印刷中 Pages: 129325-129325
  • DOI: 10.1016/j.bbagen.2019.03.011
  • Peer Reviewed
• [Journal Article] Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens (2020)
  • Author(s): Visootsat A, Nakamura A, Vignon P, Watanabe H, Uchihashi T, Iino R
  • Journal Title: The Journal of Biological Chemistry Volume: 295 Pages: 1915-1925
  • DOI: 10.1074/jbc.ra119.012078
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Non-Thermoresponsive Decanano‐sized Domains in Thermoresponsive Hydrogel Microspheres Revealed by Temperature‐Controlled High‐Speed Atomic Force Microscopy (2019)
  • Author(s): Yuichiro Nishizawa,Shusuke Matsui,Kenji Urayama,Takuma Kureha,Mitsuhiro Shibayama,Takayuki Uchihashi,Daisuke Suzuki
  • Journal Title: Angewandte Chemie International ed. Volume: 131 Pages: 8901-8905
  • DOI: 10.1002/ange.201903483
  • Peer Reviewed
• [Journal Article] Hydrogel Microellipsoids that Form Robust String-like Assemblies at the Air/Water Interface (2019)
  • Author(s): Honda Kenshiro、Sazuka Yuka、Iizuka Kojiro、Matsui Shusuke、Uchihashi Takayuki、Kureha Takuma、Shibayama Mitsuhiro、Watanabe Takumi、Suzuki Daisuke
  • Journal Title: Angewandte Chemie International ed. Volume: 印刷中 Pages: 7294-7298
  • DOI: 10.1002/anie.201901611
  • Peer Reviewed
• [Journal Article] Mutational and Combinatorial Control of Self-Assembling and Disassembling of Human Proteasome alpha Subunits (2019)
  • Author(s): Sekiguchi T., Satoh T., Kurimoto E., Song C., Kozai T., Watanabe H., Ishii K., Yagi H., Yanaka S., Uchiyama S., Uchihashi T., Murata K., Kato K.
  • Journal Title: International Journal of Molecular Sciences Volume: 20 Pages: 2308-2308
  • DOI: 10.3390/ijms20092308
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Construction of a quadrangular tetramer and a cage-like hexamer from three-helix bundle-linked fusion proteins (2019)
  • Author(s): Miyamoto Takaaki、Hayashi Yugo、Yoshida Keito、Watanabe Hiroki、Uchihashi Takayuki、Yonezawa Kento、Shimizu Nobutaka、Kamikubo Hironari、Hirota Shun
  • Journal Title: ACS Synthetic Biology Volume: 印刷中 Pages: 1112-1120
  • DOI: 10.1021/acssynbio.9b00019
  • NAID: 120006801961
  • Peer Reviewed / Open Access
• [Journal Article] The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III. (2019)
  • Author(s): [Rina Yogo,Yuki Yamaguchi,Hiroki Watanabe,Hirokazu Yagi,Tadashi Satoh,Mahito Nakanishi,Masayoshi Onitsuka,Takeshi Omasa,Mari Shimada,Takahiro Maruno,Tetsuo Torisu,Shio Watanabe,Daisuke Higo,Takayuki Uchihashi,Saeko Yanaka,Susumu Uchiyama,Koichi Kato]
  • Journal Title: Scientific Reports Volume: 9 Pages: 1-10
  • DOI: 10.1038/s41598-019-48323-w
  • NAID: 120006875664
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Protein uptake into individual hydrogel microspheres visualized by high-speed atomic force microscopy. (2019)
  • Author(s): Shusuke Matsui,Kensuke Hosho,Haruka Minato,Takayuki Uchihashi,Daisuke Suzuki
  • Journal Title: Chemical communications (Cambridge, England) Volume: 55 Pages: 10064-10067
  • DOI: 10.1039/c9cc05116c
  • Peer Reviewed
• [Journal Article] Structural basis of nucleosome assembly by the Abo1 AAA+?ATPase histone chaperone. (2019)
  • Author(s): Carol Cho,Juwon Jang,Yujin Kang,Hiroki Watanabe,Takayuki Uchihashi,Seung Joong Kim,Koichi Kato,Ja Yil Lee,Ji-Joon Song
  • Journal Title: Nature Communications Volume: 10 Pages: 5764-5764
  • DOI: 10.1038/s41467-019-13743-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy (2019)
  • Author(s): Lei Feng, Hiroki Wantanabe, Paul Molino, Gordon Wallace, Son Lam Phung, Takayuki Uchihashi, Michael Higgins
  • Journal Title: Journal of Molecular Biology Volume: 431 Pages: 2687-2699
  • DOI: 10.1016/j.jmb.2019.04.044
  • Int'l Joint Research
• [Journal Article] An Assessment of the Ability of Submicron- and Micron-Size Silicone Oil Droplets in Dropped Prefillable Syringes to Invoke Early- and Late-Stage Immune Responses (2019)
  • Author(s): Krayukhina Elena、Yokoyama Masami、Hayashihara Kayoko Kakuhou、Maruno Takahiro、Noda Masanori、Watanabe Hiroki、Uchihashi Takayuki、Uchiyama Susumu
  • Journal Title: Journal of pharmaceutical sciences Volume: 108 Pages: 2278-2287
  • DOI: 10.1016/j.xphs.2019.02.002
  • Peer Reviewed / Open Access
• [Journal Article] Microtubule self-healing and defect creation investigated by in-line force measurements during high-speed atomic force microscopy imaging (2019)
  • Author(s): Ganser Christian、Uchihashi Takayuki
  • Journal Title: Nanoscale Volume: 11 Pages: 125-135
  • DOI: 10.1039/c8nr07392a
  • Peer Reviewed
• [Journal Article] Inner lumen proteins stabilize doublet microtubules in cilia and flagella (2019)
  • Author(s): Owa M, Uchihashi T, Yanagisawa H, Yamano T, Iguchi H, Fukuzawa H, Wakabayashi K, Ando T, Kikkawa M
  • Journal Title: Nat. Commun. Volume: 10 Pages: 1143-1143
  • DOI: 10.1038/s41467-019-09051-x
  • NAID: 120006591370
  • Peer Reviewed / Open Access
• [Presentation] 高速AFMによる酸素発生光化学系II のドメイン構造揺らぎの可視化 (2020)
  • Author(s): 内橋貴之
  • Organizer: ブルカージャパン オンラインシンポジウム ～単一生体分子の動的プロセス評価に向けた高速AFM技術～
  • Invited
• [Presentation] 高速原子間力顕微鏡でタンパク質の一分子動態を可視化する (2020)
  • Author(s): 内橋貴之
  • Organizer: 新学術領域合同シンポジウム－ソフトロボット学と発動分子科学の境界
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体・人工分子のダイナミクス (2020)
  • Author(s): 内橋貴之
  • Organizer: 岡山大学大学院医歯薬学総合研究科(薬学系) 先端薬学特論 公開セミナー
  • Invited
• [Presentation] HS-AFM as a versatile tool to study dynamical and mechanical properties of proteins (2020)
  • Author(s): Christian Ganser, Kimitoshi Takeda, Ryota Iino, Koichi Kato and Takayuki Uchihashi
  • Organizer: 第58回日本生物物理学会年会: Symposium "The Multiple Modes of Proteins ? From Molecular Dynamics to Pathogenesis -
  • Int'l Joint Research / Invited
• [Presentation] 高速原子間顕微鏡による膜輸送装置Secの動態観察 (2020)
  • Author(s): 長池航, 板家成良, 春山隆充, 塚崎智也, 内橋貴之
  • Organizer: 日本生体エネルギー研究会第46回討論会
• [Presentation] 高速原子間力顕微鏡を用いた光化学系IIの動態観察 (2020)
  • Author(s): 戸叶貴也, 加藤祐樹, 杉山翔吾, 野口巧, 内橋貴之
  • Organizer: 日本生体エネルギー研究会第46回討論会
• [Presentation] Observation of Substrate Binding Sec Translocon and Structural Change of SecA with HS-AFM (2020)
  • Author(s): Wataru Nagaike, Takamitsu Haruyama, Tomoya Tsukazaki and Takayuki Uchihashi
  • Organizer: 第58回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] 溶液中HS-AFM observation of Amyloid β elongation and inhibition by antibodies (2020)
  • Author(s): Shogo Miyajima, Maho Yagi-Utsumi, Takayuki Uchihashi and Koichi Kato
  • Organizer: 第58回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] Chasing the transformation between monomer and dimer structure of cadherin anchored to supported lipid bilayer by high-speed AFM (2020)
  • Author(s): Shigetaka Nishiguchi, Hiroki Oda and Takayuki Uchihashi
  • Organizer: 第58回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] Imaging of Single-Molecule Dynamics using High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: The 2nd NIBB-Princeton Joint Symposium on Imaging and Quantitative Biology
  • Int'l Joint Research / Invited
• [Presentation] Real-Time Visualization of Biological Molecules at Work with High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale 2019
  • Int'l Joint Research / Invited
• [Presentation] Nanoscale Dynamic Imaging of Biological Molecules with High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: Frontier Bioorganization Forum 2019
  • Int'l Joint Research / Invited
• [Presentation] High-Speed Atomic Force Microscopy for Direct Visualization from Single Protein to Cell Dynamics (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: EsCELLS visit talks
  • Int'l Joint Research / Invited
• [Presentation] Defect creation in microtubules and kinesin motility studied by HS-AFM (2019)
  • Author(s): Christian Ganser, Kimitoshi Takeda, Ryota Iino, Koichi Kato and Takayuki Uchihashi
  • Organizer: 27th International Colloquium on Scanning Probe Microscopy (ICSPM27)
  • Int'l Joint Research
• [Presentation] Real-Time Monitoring of Morphological Change of Metal Surface During Corrosion Process with High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi, Mitsuhiro Okada, Miyuki Tanaka, Toshitaka Kubo, Tetsuo Shimizu
  • Organizer: 27th International Colloquium on Scanning Probe Microscopy (ICSPM27)
  • Int'l Joint Research
• [Presentation] Kinesin transport on microtubules studied by HS-AFM (2019)
  • Author(s): Christian Ganser and Takayuki Uchihashi
  • Organizer: The 2nd NIBB-Princeton Joint Symposium on Imaging and Quantitative Biology
  • Int'l Joint Research
• [Presentation] Dynamics of photosystem II protein complexes as observed by high-speed atomic force microscopy (2019)
  • Author(s): Takaya Tokano, Yuki Kato, Shogo Sugiyama, Takumi Noguchi, Takayuki Uchihashi
  • Organizer: The 2nd NIBB-Princeton Joint Symposium on Imaging and Quantitative Biology
  • Int'l Joint Research
• [Presentation] 高速原子間力顕微鏡と生体・人工分子のダイナミクス計測 (2019)
  • Author(s): 内橋貴之
  • Organizer: 第23回コロイド・界面技術者フォーラム～コロイド領域のイメージング・評価技術～
  • Invited
• [Presentation] ダイナミンの動的分子イメージング：in vitro 再構成系×高速 AFM (2019)
  • Author(s): 竹居孝二, 内橋貴之
  • Organizer: ワークショップ「新しいコラボの在り方を探る～細胞生物学ｘ蛋白質科学」 第19回 日本蛋白質科学会年会, 第71回 日本細胞生物学会大会 合同年次大会
  • Invited
• [Presentation] 溶液中高速原子間力顕微鏡による生体・人工分子のダイナミクス解析 (2019)
  • Author(s): 内橋貴之
  • Organizer: シンポジウム「原子間力顕微鏡技術のパラダイムシフト～原子から細胞まで階層をまたぐ構造・機能解析～」 日本顕微鏡学会 第75回学術講演会
• [Presentation] 高速原子間力顕微鏡による生細胞イメージング (2019)
  • Author(s): 内橋貴之
  • Organizer: 日本学術振興会 ナノプローブテクノロジー第167委員会 第91回研究会
  • Invited
• [Presentation] Dynamic Structural Analysis of Proteins at Work with High-Speed Atomic Force Microscopy (2019)
  • Author(s): 内橋貴之
  • Organizer: 【応用物理学界 関西支部】支部セミナー「バイオフォトニクス研究の最前線」
  • Invited
• [Presentation] Visualization of Single Molecule Dynamics at Work with High-Speed Atomic Force Microscopy (2019)
  • Author(s): 内橋貴之
  • Organizer: 1st ITbM Frontier Seminar
  • Invited
• [Presentation] Nanoscale Dynamic Imaging of Biological Molecules with High-Speed Atomic Force Microscopy (2019)
  • Author(s): Takayuki Uchihashi
  • Organizer: 第５回Neuroscience Network in Kobe -微細構造イメージングと神経科学との接点を探る-
  • Invited
• [Presentation] 高速原子間力顕微鏡で可視化する分子ダイナミクス (2019)
  • Author(s): 内橋貴之
  • Organizer: 日本学術振興会 情報科学用有機材料第142委員会 合同研究会
  • Invited
• [Presentation] Kinesin movement around defects in the microtuble lattice monitored by high-speed atomic force microscopy (2018)
  • Author(s): Christian Ganser and Takayuki Uchihashi
  • Organizer: The 79th Okazaki Conference "Synthetic, Biological, and Hybrid Molecular Engines
  • Int'l Joint Research
• [Presentation] In-situ observation of self-assembling fiber formation of biological and artificial molecules (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: ACSIN-14 & ICSPM26, Symposium: Nanobiology
  • Int'l Joint Research
• [Presentation] Defects in Microtubules Studied by High-Speed Atomic Force Microscopy (2018)
  • Author(s): Christian Ganser and Takayuki Uchihashi
  • Organizer: ACSIN-14 & ICSPM26
  • Int'l Joint Research
• [Presentation] Direct observation of self-assembly process of biological and artificial fibrils using high-speed atomic force microscopy (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: Interhierarchical understanding of materials and life through molecular observation
  • Int'l Joint Research / Invited
• [Presentation] High-Speed Atomic Force Microscopy for Visualization of Dynamic Processes in Biological and Artificial Supramolecules (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: International Scanning Probe Microscopy 2018
  • Int'l Joint Research / Invited
• [Presentation] Dynamic Structural States of Molecular Disaggregation Machine ClpB Revealed by High-Speed Atomic Force Microscopy (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: Frontier Bioorganization Forum 2018
  • Int'l Joint Research / Invited
• [Presentation] Extending the functionality of high-speed Atomic Force Microscopy: In-line force measurements to study microtubules (2018)
  • Author(s): Christian Ganser
  • Organizer: Minisymposium on “Advanced Atomic Force Microscopy”
  • Int'l Joint Research / Invited
• [Presentation] High-speed Atomic Force Microscopy: A tool for direct visualization of single-molecule dynamics", (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: Minisymposium on “Advanced Atomic Force Microscopy”
  • Int'l Joint Research / Invited
• [Presentation] Imaging and Manipulation of Biological Molecules with High-Speed Atomic Force Microscopy (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: ICN-T 2018
  • Int'l Joint Research / Invited
• [Presentation] High-Speed Atomic Force Microscopy for Visualization and Manipulation of Biological and Artificial Molecules (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: SPMonSPM 2018
  • Int'l Joint Research / Invited
• [Presentation] High-speed atomic force microscopy for direct visualization of biological macromolecules at work (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: The 79th Okazaki Conference "Synthetic, Biological, and Hybrid Molecular Engines",
  • Int'l Joint Research / Invited
• [Presentation] Connection between AFM data and computational simulation - image processing for quantitative analysis and demands from the experimental side - (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: Trends in Computational Molecular Biophysics Workshop,
  • Int'l Joint Research / Invited
• [Presentation] Conformational dynamics relevant to disaggregation activity of ClpB visualized by high-speed atomic force microscopy (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: RIKEN Symposium Recent Progress in Protein Conformation and Aggregation
  • Int'l Joint Research / Invited
• [Presentation] 高速原子間力顕微鏡で可視化する生体分子のナノ動態 (2018)
  • Author(s): 内橋貴之
  • Organizer: NSIセミナー・アドバンス生命理学特論
  • Invited
• [Presentation] 高速原子間力顕微鏡を用いた溶液環境下での分子のダイナミクス計測 (2018)
  • Author(s): 内橋貴之
  • Organizer: 平成30年度実践セミナー 『光・ナノ計測実践セミナーⅢ』
  • Invited
• [Presentation] 高速AFMの開発とタンパク質の動態計測 (2018)
  • Author(s): 内橋貴之
  • Organizer: 第58回生物物理若手の会
  • Invited
• [Presentation] 原子間力顕微鏡でリアルタイム可視化する生体/人工超分子の重合ダイナミクス (2018)
  • Author(s): 内橋貴之
  • Organizer: 日本物理学会 2018年秋季大会 領域9, 5合同シンポジウム「時間分解プローブを駆使した表面・界面科学及び結晶成長の進展と展望 」
  • Invited
• [Presentation] Image Processing and Quantitative Analysis of High-Speed-AFM Data for Studying Single-Molecule Dynamics (2018)
  • Author(s): Takayuki Uchihashi
  • Organizer: 第56回日本生物物理学会年会, Symposium on "Multiple Approaches for Analyses of Protein Complexes -Methods and Applications"
  • Int'l Joint Research
• [Presentation] 高速AFMで読み解く生体試料のマルチスケール動態 (2018)
  • Author(s): 内橋貴之
  • Organizer: 第１回 ExCELLSシンポジウム
  • Invited
• [Book] 膜タンパク質工学ハンドブック:第1編 第2章15「高速原子間力顕微鏡によるタンパク質の構造ダイナミクス解析」 (2020)
  • Author(s): 内橋貴之
  • Total Pages: 624
  • Publisher: 株式会社 エヌ・ティー・エス
  • ISBN: 9784860435370
• [Book] 自己修復材料、 自己組織化、形状記憶材料の開発と応用事例 (2020)
  • Author(s): 内橋貴之
  • Total Pages: 446
  • Publisher: 技術情報協会
  • ISBN: 9784861047817
• [Book] "High-Speed Atomic Force Microcopy" in Compendium of Surface and Interface Analysis (2018)
  • Author(s): Takayuki Uchihashi
  • Total Pages: 853
  • Publisher: Springer
  • ISBN: 9789811061554
• [Book] 実験医学増刊 Vol.36 No.20, 「生きてるものは全部観る！ イメージングの選び方・使い方100」(原田慶恵，永井健治 編)： 第5章 走査型プローブ顕微鏡「原子間力顕微鏡 ⅰ．高速原子間力顕微鏡」 (2018)
  • Author(s): 内橋貴之
  • Total Pages: 225
  • Publisher: 羊土社,
• [Remarks] 名古屋大学D研ホームページ
  • URL: http://d.phys.nagoya-u.ac.jp/
• [Remarks] 名古屋大学D研ホームページ
  • URL: http://d.phys.nagoya-u.ac.jp/index.html