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Next generation DNA sequencing using surface-enhanced Raman spectroscopy

Research Project

Project/Area Number 18H01847
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 28050:Nano/micro-systems-related
Research InstitutionKobe University

Principal Investigator

Sugano Koji  神戸大学, 工学研究科, 准教授 (20372568)

Co-Investigator(Kenkyū-buntansha) 磯野 吉正  神戸大学, 工学研究科, 教授 (20257819)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords表面増強ラマン分光法 / 1分子計測 / 金ナノ粒子 / プラズモニクス / セルフアセンブル / 表面増強ラマン分光 / DNAシーケンシング
Outline of Final Research Achievements

This study reports the detection and the identification of a single base from a single DNA oligomer by surface-enhanced Raman spectroscopy (SERS) using a gold nanoparticle dimer. The gold nanoparticle dimer structure was fabricated by a self-assembling process using a nanotrench. A Raman spectrum derived from an 8-chain DNA oligomer with one adenine and seven cytosines was obtained. It was confirmed that a single DNA oligomer was detected from a single gold nanoparticle dimer. It indicates that a single adenine was detected. In addition, only the adenine peak without the cytosine peak was observed, indicating the possibility of spatial resolution with a single DNA base.

Academic Significance and Societal Importance of the Research Achievements

表面増強ラマン分光(SERS:Surface Enhanced Raman Spectroscopy)の高い分子構造同定能力と高い感度を利用した新規なDNAシーケンシングのために,1塩基検出が可能なSERS計測技術を構築した。これによりSERSにおけるナノギャップの光電磁場増強効果の理解が進めるとともに,DNA計測のための基盤技術を構築した。この技術はDNAシーケンシングへ期待できるとともに様々な1分子計測基盤技術としての波及効果をもたらす。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (21 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 7 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] Seoul National University(韓国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Seoul National University(韓国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Dynamic electrical measurement of biomolecule behavior via plasmonically-excited nanogap fabricated by electromigration2021

    • Author(s)
      Akihiro Morita, Takayuki Sumitomo, Akio Uesugi, Koji Sugano, Yoshitada Isono
    • Journal Title

      Nano Express

      Volume: 2 Issue: 1 Pages: 010032-010032

    • DOI

      10.1088/2632-959x/abe9c0

    • NAID

      120007097074

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Polarization Dependence of Near-infrared Absorption Spectrum of Si-deposited Gold Nano-grating Structures2020

    • Author(s)
      新居直之,上杉晃生,菅野公二,磯野吉正
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 140 Issue: 4 Pages: 72-78

    • DOI

      10.1541/ieejsmas.140.72

    • NAID

      130007825102

    • ISSN
      1341-8939, 1347-5525
    • Year and Date
      2020-04-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of wavelength shift of near-infrared laser using mechanical microresonator-based sensor with Si-covered gold nanorods as optical absorber2020

    • Author(s)
      Koji Sugano, Yuki Tanaka, Akio Uesugi, Etsuo Maeda, Reo Kometani, Yoshitada Isono
    • Journal Title

      Sensors and Actuators A: Physical

      Volume: 315 Pages: 112337-112337

    • DOI

      10.1016/j.sna.2020.112337

    • NAID

      120006993689

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wavelength-dependent near-infrared microbolometer for short-wavelength infrared light with gold nanowire grating optical absorber2020

    • Author(s)
      Tatsuya Tsubota, Akio Uesugi, Koji Sugano, Yoshitada Isono
    • Journal Title

      Microsystem Technologies

      Volume: 27 Issue: 3 Pages: 997-1005

    • DOI

      10.1007/s00542-020-05004-3

    • NAID

      120007002612

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of clamped beam pattern on resonant frequency shift of microresonator under near-infrared laser irradiation2020

    • Author(s)
      Kohei Takegami, Kota Nakafuji, Naoyuki Arai, Akio Uesugi, Koji Sugano, and Yoshitada Isono
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: SI Pages: SIII04-SIII04

    • DOI

      10.35848/1347-4065/ab8412

    • NAID

      120006877473

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strain engineering of core-shell silicon carbide nanowires for mechanical and piezoresistive characterizations2019

    • Author(s)
      Shinya Nakata, Akio Uesugi , Koji Sugano, Francesca Rossi, Giancarlo Salviati, Alois Lugstein and Yoshitada Isono
    • Journal Title

      Nanotechnology

      Volume: 30 Issue: 26 Pages: 265702-265702

    • DOI

      10.1088/1361-6528/ab0d5d

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Electrical and Optical Characterization of Nanogap Electrodes with an Assembled Gold Nanoparticle Chain2020

    • Author(s)
      Takayuki Sumitomo, Akihiro Morita, Akio Uesugi, Koji Sugano and Yoshitada Isono
    • Organizer
      33rd International Microprocesses and Nanotechnology Conference (MNC2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 金ナノ粒子二量体を用いたナノギャップ電極の作製とその電気的・光学的評価2020

    • Author(s)
      住友 孝行, 森田 明宏, 上杉 晃生, 菅野 公二, 磯野 吉正
    • Organizer
      第37回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] Manipulation of Biomolecules Into Nanogap by Plasmonic Optical Excitation for Highly Sensitive Biosensing2019

    • Author(s)
      Akihiro Morita, Akio Uesugi, Koji Sugano, and Yoshitada Isono
    • Organizer
      The 20th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SERS Detection of a Single Nucleobase in a DNA Oligomer Using a Gold Nanoparticle Dimer2019

    • Author(s)
      Koji Sugano, Katsunari Maruoka, Akio Uesugi, and Yoshitada Isono
    • Organizer
      The 20th International Conference onSolid-State Sensors, Actuators and Microsystems
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of Gold Nanoparticle Diameter on Raman Intensity of DNA Oligomers toward Single Nucleobase Detection2019

    • Author(s)
      Kenji Murotani, Katsunari Maruoka, Akio Uesugi, Koji Sugano, Yoshitada Isono
    • Organizer
      32nd International Microprocesses and Nanotechnology Conference (MNC 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Surface-Enhanced Raman Spectroscopy of DNA with Single-Molecule Sensitivity Using Gold Nanoparticle Dimer2019

    • Author(s)
      Koji Sugano
    • Organizer
      The 19th IEEE International Conference on Nanotechnology (IEEE-NANO 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-molecule Surface Enhanced Raman Spectroscopy Using Gold Nanoparticle Dimer2019

    • Author(s)
      Koji Sugano
    • Organizer
      2019 International Conference on Optical MEMS and Nanophotonics (IEEE OMN 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 金ナノ粒子二量体表面増強ラマン分光によるDNAオリゴマーの1塩基検出2019

    • Author(s)
      金谷恭臣,丸岡克成,森田明宏,上杉晃生,菅野公二,磯野吉正
    • Organizer
      第36回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 光励起ナノギャップ電極を用いたDNA オリゴマーの光トラップおよび1分子検出2019

    • Author(s)
      森田明宏,上杉晃生,菅野公二,磯野吉正
    • Organizer
      第36回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Surface-Enhanced Raman Spectroscopy of DNA Bases Using Gold Nanoparticle Dimer Array2018

    • Author(s)
      Katsunari Maruoka, Kohei Ikegami, Akio Uesugi, Koji Sugano, Yoshitada Isono
    • Organizer
      31st International Microprocesses and Nanotechnology Conference (MNC2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Si薄膜被覆金ナノグレーティング構造の近赤外域光吸収スペクトル偏光依存性2018

    • Author(s)
      新居直之, 上杉晃生, 菅野公二, 磯野吉正
    • Organizer
      第34回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Remarks] http://www.research.kobe-u.ac.jp/eng-isonolab/

    • Related Report
      2019 Annual Research Report
  • [Remarks]

    • URL

      http://www.research.kobe-u.ac.jp/eng-isonolab/index.html

    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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