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Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

Research Project

Project/Area Number 18H02008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 34020:Analytical chemistry-related
Research InstitutionKeio University

Principal Investigator

チッテリオ ダニエル  慶應義塾大学, 理工学部(矢上), 教授 (00458952)

Co-Investigator(Kenkyū-buntansha) 蛭田 勇樹  慶應義塾大学, 理工学部(矢上), 講師 (60710944)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords抗体医薬品 / POCT / 糸基板センサー / 生物発光 / マイクロ流体
Outline of Annual Research Achievements

病院の診療室やベッドサイド、さらには在宅で行う検査、すなわちPoint of Careで、1滴の血液から血中抗体医薬品濃度のモニタリングができる安価、簡便、使い捨ての糸基板マイクロ流体分析デバイス(microfluidic thread-based analytical devices; μTADs)を開発した。
これまでに、簡便な抗体定量検出が可能な生物発光酵素タンパク質、LUMABS (LUMinescent AntiBody Sensor) をμTADsに応用することに成功しており、今年度は、Point of Careでの分析のために、カメラやスマートフォンでデバイスの生物発光シグナルを撮影することで定量分析を行う方法について研究を行った。カメラやスマートフォンを使用した定量分析を行うため、イタリアの研究者と共に分析方法を検討し、測定と最適化を行うことにより、新たな画像解析方法を開発した。その結果、標準偏差の少ない、実際の臨床検査でも使用可能なデータを得ることができた。この成果については、Sensors and Actuators B: Chemicalに投稿し、いくつかの国際学会で発表を行った。また、フリマジンの安定性についての論文を、Organic & Biomolecular Chemistryに投稿した。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(26 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (16 results) (of which Int'l Joint Research: 13 results,  Invited: 9 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] Eindhoven University of Technology(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Genova(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Eindhoven University of Technology(オランダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Department of Biomedical Engineering/Eindhoven University of Technology(オランダ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Microfluidic thread-based analytical devices for point-of-care detection of therapeutic antibody in blood2022

    • Author(s)
      Riho Shimazu, Kosuke Tomimuro, Yan Ni, Cristina Malegori, Vahid Hamedpour, Yuki Hiruta, Paolo Oliveri, Maarten Merkx, Daniel Citterio
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 352

    • DOI

      10.1016/j.snb.2021.131002

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues2021

    • Author(s)
      Mizui Yuki、Eguchi Masatoshi、Tanaka Masanobu、Ikeda Yuma、Yoshimura Hideaki、Ozawa Takeaki、Citterio Daniel、Hiruta Yuki
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 19 Pages: 579-586

    • DOI

      10.1039/d0ob02020f

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thread-Based Multiplex Bioluminescent Antibody Detection in a Single Drop of Whole Blood2020

    • Author(s)
      Kosuke Tomimuro, Keisuke Tenda, Yan Ni, Yuki Hiruta, Maarten Merkx, Daniel Citterio
    • Journal Title

      ACS Sensors

      Volume: ー

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Paper-Based Antibody Detection Devices Using Bioluminescent BRET-Switching Sensor Proteins2018

    • Author(s)
      Tenda Keisuke、van Gerven Benice、Arts Remco、Hiruta Yuki、Merkx Maarten、Citterio Daniel
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 57 Pages: 15369-15373

    • DOI

      10.1002/anie.201808070

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microfluidic thread-based analytical devices for antibody detection in whole blood using bioluminescent sensor proteins2018

    • Author(s)
      Tomimuro Kosuke, Tenda Keisuke, Hiruta Yuki, Merkx Maarten, Citterio Daniel
    • Journal Title

      Proceedings of MicroTAS 2018

      Volume: なし Pages: 1018-1021

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 化学・生化学センシングのための機能性色素および紙基板分析デバイスの開発2022

    • Author(s)
      Daniel Citterio
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Paper-based analytical devices (PADs) Ultimate Simplicity2021

    • Author(s)
      Daniel Citterio
    • Organizer
      Asianalysis: Asian Conference on Analytical Sciences
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analytical assays on paper and thread platforms: as simple as it can get2021

    • Author(s)
      Daniel Citterio
    • Organizer
      JSPS International Workshop on Symbiosis of Biology and Nanodevices
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Paper-based analytical devices for naked-eye (semi)quantitative signal readout2021

    • Author(s)
      Daniel Citterio
    • Organizer
      Pacifichem: International Chemical Congress of Pacific Basin Societies
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 抗体検出のための生物発光タンパク質を用いたマイクロ流体糸基板型分析デバイスの開発2019

    • Author(s)
      Kosuke Tomimuro, Keisuke Tenda, Yuki Hiruta, Maarten Merkx, Daniel Citterio
    • Organizer
      日本分析化学会関東支部若手交流会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Thread-Based Analytical Device for Antibody Detection in Whole Blood by Using Bioluminescent Sensor Proteins2019

    • Author(s)
      Kosuke Tomimuro, Keisuke Tenda, Yuki Hiruta, Maarten Merkx, Daniel Citterio
    • Organizer
      RSC Tokyo International Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic Thread-Based Analytical Devices for Point-of-Care Detection of Therapeutic Antibodies in Blood2019

    • Author(s)
      Riho Shimazu, Kosuke Tomimuro, Yan Ni, Yuki Hiruta, Maarten Merkx, Daniel Citterio
    • Organizer
      RSC Tokyo International Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic Thread-Based Analytical Devices for Point-of-Care Detection of Therapeutic Antibodies in Blood2019

    • Author(s)
      Riho Shimazu, Kosuke Tomimuro, Yan Ni, Yuki Hiruta, Maarten Merkx, Daniel Citterio
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Microfluidic Paper-Based Analytical Devices - More than Simple Test Papers2019

    • Author(s)
      Daniel Citterio
    • Organizer
      第29回日本MRS年次大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Step Bioluminescence Lateral Flow Immunoassays for Diagnostics2019

    • Author(s)
      Riho Shimazu, Junnosuke Kawahara, Kosuke Tomimuro, Kazushi Misawa, Yan Ni, Yuki Hiruta, Maarten Merkx, Daniel Citterio
    • Organizer
      23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Paper- and thread-based immunoassay devices with colorimetric bioluminescence signaling2019

    • Author(s)
      Tomimuro Kosuke, Tenda Keisuke, Merkx Maarten, Hiruta Yuki, Citterio Daniel
    • Organizer
      Pittcon 2019, Philadelphia, USA
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Paper-based analytical device for the detection of antibodies in biological samples2018

    • Author(s)
      Tenda Keisuke、van Gerven Benice、Arts Remco、Hiruta Yuki、Merkx Maarten、Citterio Daniel
    • Organizer
      PERCH-CIC 2018, Pattaya, Thailand
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Simple and low-cost optical detection of clinically relevant targets at point-of-care2018

    • Author(s)
      Citterio Daniel
    • Organizer
      International Workshop on Photonics Polymer for Innovation (IWPPI), Suwa, Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of stable bioluminescent substrates activatable by hydrolysis2018

    • Author(s)
      Mizui Yuki, Tanaka Masanobu, Tenda Keisuke, Iwasawa Naoko, Hiruta Yuki, Citterio Daniel
    • Organizer
      Royal Society of Chemistry - Tokyo International Conference 2018 (RSC-TIC 2018), Makuhari Messe, Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic thread-based analytical devices for antibody detection in whole blood using bioluminescent sensor proteins2018

    • Author(s)
      Tomimuro Kosuke, Tenda Keisuke, Hiruta Yuki, Merkx Maarten, Citterio Daniel
    • Organizer
      International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), Kaohsiung, Taiwan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Capillary flow-driven analytical devices for sample-in-signal-out assaying of clinically relevant analytes2018

    • Author(s)
      Citterio Daniel
    • Organizer
      Flow Analysis 2018, Bangkok, Thailand
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Patent(Industrial Property Rights)] Detection device for bioluminescent detection of biomarkers from a biological fluid sample using luminescent sensing proteins2018

    • Inventor(s)
      Citterio, Merkx et al.
    • Industrial Property Rights Holder
      Citterio, Merkx et al.
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
    • Overseas

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Published: 2018-04-23   Modified: 2022-12-28  

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