Diriving motor proteins by photoisomerization reactions
| Project/Area Number |
18H02042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 35030:Organic functional materials-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | アゾベンゼン / モータータンパク質 / 光異性化反応 / フォトクロミズム / キネシン / ミオシン / ダイニン / 微小管 / クラミドモナス / 分子スイッチ / 光薬理学 / 分子機械 / 光駆動 / 光スイッチ / ATP |
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| Outline of Final Research Achievements |
We clarified the structure-activity relationship of photoresponsive high-energy compounds that drive motor proteins, and discovered compounds that distinguish and drive kinesin and myosin. We have synthesized a photoresponsive inhibitor to a motor protein that arranges chromosomes in the process of the cell cycle, and succeeded in photoswitching the arrangement of chromosomes in the cell. We synthesized a photoresponsive inhibitor that acts on dynein and succeeded in photocontrolling the movement of Chlamydomonas using flagella. Furthermore, we succeeded in replacing the retinal of the proton pump with an azobenzene derivative, and confirmed the photocycle and photoinduced proton transfer. We also succeeded in creating a completely artificial molecular system that converts light energy into continuous work in contrast to proteins.
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| Academic Significance and Societal Importance of the Research Achievements |
キネシンとミオシンに対して選択的に活性を示す光応答性高エネルギー化合物を発見したことは、モータータンパク質がその種類による基質認識様式の違いを示唆し、モータータンパク質の運動の分子メカニズム関する基礎研究における重要な知見となる。モータータンパク質CENP-Eやダイニンに対して働く光応答性阻害剤によってタンパク質の運動機能のみならず細胞周期や生物の運動を光制御できたことは、細胞の働きを望みの場所、望みのタイミングでスイッチしうること意味するので、今後、副作用のない抗がん剤などへと応用が可能である。
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Substrate selectivity and its mechanistic insight of the photo-responsive non-nucleoside triphosphate for myosin and kinesin2019
Author(s)
Islam, Md. J., Matsuo, K., Menezes, H. M., Takahashi, M., Nakagawa, H., Kakugo, A., Sada, K., Tamaoki, N.
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Journal Title
Organic & Biomolecular Chemistry
Volume: 17
Issue: 1
Pages: 53-65
DOI
Related Report
Peer Reviewed
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