|Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|Outline of Final Research Achievements
In this study, we developed a molecular tool combining a photosensitizer and a peptide/protein (functional photosensitizing peptide; FPP) to establish a technology for the spatiotemporal control and observation of cellular functions by light. First, we elucidated the effects of the amino acid sequence near the photosensitizers to support FPP design. Furthermore, by using this technology, we established a method for light-directed cytosolic delivery of two different RNAs, clarified the cell cycle dependence of apoptosis photo-triggered using a FPP, and succeeded in photoinduced RNA delivery into a single cell in mouse embryos.