Advanced 3D neuropathology based on whole-mount tissue staining methods
| Project/Area Number |
18H02105
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 透明化 / 3Dイメージング / 神経病理 / ケミカルプローブ |
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| Outline of Final Research Achievements |
Current biopsy and histology have long relied on thin-sectioned 2D images with several chemical staining methods and specific immunohistochemistry. Facile 3D visualization of human brain tissue with single-cell resolution would provide a novel concept of the neuropathological diagnosis and contribute our understanding of pathological mechanisms based on comprehensive and quantitative analysis of individual biomarker. In this project, we aimed at establishing a novel 3D neuropathology by developing highly efficient clearing and staining protocol for human brain tissue and combining with a rapid 3D imaging using light-sheet fluorescence microscopy. As a result, we developed versatile immunohistochemical method and a series of chemical probes for vasculature, cytoplasm, and senile plaques in human brain tissue.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は透明化処理に適用可能な蛍光プローブを探索・設計するための一般的な化学的概念の創出を目的としており、先駆的な研究課題である。これにより、有機化学者がヒト脳剖検サンプルを用いた蛍光プローブ開発を展開するための基盤を確立できるため、化学と医学の間の壁を突破するケミカルメディシンを拓く一つのマイルストーンになりえる。また、3次元神経病理学が確立されることにより、「定量的な診断基準の確立」・「病変部位の網羅的探索」・「立体構造の異常に基づく疾患概念の体系化」が期待され、神経病理学分野の発展に大きく寄与できる。
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Spatiotemporal dynamics of clonal selection and diversification in normal endometrial epithelium.2022
Author(s)
Yamaguchi M, Nakaoka H, Suda K, Yoshihara K, Ishiguro T, Yachida N, Saito K, Ueda H, Sugino K, Mori Y, Yamawaki K, Tamura R, Revathidevi S, Motoyama T, Tainaka K, Verhaak RGW, Inoue I, Enomoto T.
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Journal Title
Nat Commun.
Volume: 13
Pages: 943
DOI
Related Report
Peer Reviewed
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[Journal Article] Mechanical load regulates bone growth via periosteal Osteocrin.2021
Author(s)
Watanabe-Takano H, Ochi H, Chiba A, Matsuo A, Kanai Y, Fukuhara S, Ito N, Sako K, Miyazaki T, Tainaka K, Harada I, Sato S, Sawada Y, Minamino N, Takeda S, Ueda HR, Yasoda A, Mochizuki N.
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Journal Title
Cell Rep.
Volume: 36
Pages: 109380
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Three-dimensional understanding of the morphological complexity of the human uterine endometrium2021
Author(s)
Yamaguchi M, Yoshihara K, Suda K, Nakaoka H, Yachida N, Ueda H, Sugino K, Mori Y, Yamawaki K, Tamura R, Ishiguro T, Motoyama T, Watanabe Y, Okuda S, Tainaka K, Enomoto T.
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Journal Title
iScience
Volume: 24
Pages: 102258
DOI
Related Report
Peer Reviewed
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[Journal Article] Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues.2020
Author(s)
Susaki EA, Shimizu C, Kuno A, Tainaka K, Li X, Nishi K, Morishima K, Ono H, Ode KL, Saeki Y, Miyamichi K, Isa K, Yokoyama C, Kitaura H, Ikemura M, Ushiku T, Shimizu Y, Saito T, Saido TC, Fukayama M, Onoe H, Touhara K, Isa T, Kakita A, Shibayama M, Ueda HR.
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Journal Title
Nat. Commun.
Volume: 11
Pages: 1982
DOI
Related Report
Peer Reviewed
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[Journal Article] Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.2018
Author(s)
Tainaka K, Murakami TC, Susaki EA, Shimizu C, Saito R, Takahashi K, Hayashi-Takagi A, Sekiya H, Arima Y, Nojima S, Ikemura M, Ushiku T, Shimizu Y, Murakami M, Tanaka KF, Iino M, Kasai H, Sasaoka T, Kobayashi K, Miyazono K, Morii E, Isa T, Fukayama M, Kakita A, Ueda HR.
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Journal Title
Cell Rep.
Volume: 24
Pages: 2196-2210.e9
DOI
Related Report
Peer Reviewed / Open Access
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