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A multidisciplinary research program to enable the discovery of inhibitors of colanic acid excretion in bacteria

Research Project

Project/Area Number 18H02109
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 37030:Chemical biology-related
Research InstitutionKagawa University

Principal Investigator

KONG Lingbing  香川大学, 農学部, 助教 (20798384)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
KeywordsAntibacterial / Colanic acid / Wza / Single-molecule / single-molecule / antibacterial / single-moleule / colanic acid / inhibitor / Inhibitor / bacteria / excretion
Outline of Final Research Achievements

Three pathogenic bacterial strains have been cultured and stored. WzaCA is transporter of colonic acid. Genomic DNA extraction followed by polymerase chain reaction and cloning onto desired vectors has enabled the expression of WzaCA. Meanwhile, chemical strategies have been developed for efficient production of potential blockers. A LC-MS system equipped with autosampler, fraction collector, temperature controller and multiple detectors which include UV/Vis, 3D-field, refractive index, high-resolution mass, etc. has been established for fast and accurate access to various potential blockers. Lastly, the proposed microscopic system equipped with an ideal EMCCD camera has been set up. Further screening of blockers is currently ongoing. Once a desired blocker is identified in single-molecule electrical recordings, all sorts of biochemical, biophysical and biological tests that have been established will be carried out. Results will be published in due course.

Academic Significance and Societal Importance of the Research Achievements

Antibacterial resistance is a global challenge. Novel antibacterial strategies and targets are urgently needed. Colanic acid (CA) is one of outermost and most essential outer bacterial matrices in the formation of most biofilms. Inhibition of CA export will help eradicate biofilms.

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (4 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Revelation of Function and Inhibition of Wza Through Single-Channel Studies2021

    • Author(s)
      Kong Lingbing
    • Journal Title

      Methods Mol. Biol.

      Volume: 2186 Pages: 63-76

    • DOI

      10.1007/978-1-0716-0806-7_6

    • ISBN
      9781071608050, 9781071608067
    • Related Report
      2020 Annual Research Report
  • [Journal Article] Revelation of function and inhibition of Wza through single channel studies2020

    • Author(s)
      Lingbing Kong
    • Journal Title

      Methods in Molecular Biology, in press

      Volume: x

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Revelation of function and inhibition of Wza through single channel studies2019

    • Author(s)
      Lingbing Kong
    • Journal Title

      Methods in Molecular Biology

      Volume: x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Interrogation of a bacterial sugar transporter for novel biomedicines and biotechnologies2018

    • Author(s)
      Lingbing Kong
    • Organizer
      Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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