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Regulation mechanism of iron nutrition by ubiquitin ligases and iron-sulfur clusters

Research Project

Project/Area Number 18H02115
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionIshikawa Prefectural University

Principal Investigator

Kobayashi Takanori  石川県立大学, 生物資源環境学部, 教授 (70590206)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Keywords鉄欠乏 / ユビキチンリガーゼ / 鉄硫黄クラスター / イネ / 発現制御
Outline of Final Research Achievements

We conducted in vitro analysis for putative substrates of rice HRZ ubiquitin ligases, including glutaredoxins (GRX), and bZIP and bHLH transcription factors. We found that GRXs and bZIP are subjected to HRZ-dependent degradation via the 26S proteasome pathway. We also produced and analyzed transgenic rice plants overexpressing or repressing these genes or iron deficiency-inducible IMA peptide genes. We found that GRXs and bZIP are novel downstream factors for HRZs, and are facilitating iron utilization in rice plants. We also found that bHLH and IMAs are novel positive regulators for rice iron deficiency responses.

Academic Significance and Societal Importance of the Research Achievements

本研究により、イネにおいてHRZ が鉄の吸収や蓄積を抑える際に、GRX、bZIP転写因子、bHLH転写因子、IMAペプチドを介して鉄の輸送や吸収を調節していることが明らかになった。また、植物細胞内における鉄欠乏の感知とシグナル伝達に関する知見が得られた。本研究の知見は、HRZ の働きを適切に調節し、鉄分を吸いにくい土壌でも良好に生育する植物、鉄分が豊富な作物を創出することに貢献すると期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (34 results)

All 2022 2021 2020 2019 2018

All Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Open Access: 3 results) Presentation (20 results) (of which Int'l Joint Research: 5 results,  Invited: 7 results) Book (4 results)

  • [Journal Article] 10. Mechanism of iron transport and its regulation in plants2021

    • Author(s)
      小林 高範
    • Journal Title

      Japanese Journal of Soil Science and Plant Nutrition

      Volume: 92 Issue: 2 Pages: 141-147

    • DOI

      10.20710/dojo.92.2_141

    • NAID

      130008024140

    • ISSN
      0029-0610, 2424-0583
    • Year and Date
      2021-04-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of subcellular metal homeostasis in crop improvement2021

    • Author(s)
      Bashir Khurram、Ahmad Zarnab、Kobayashi Takanori、Seki Motoaki、Nishizawa Naoko K
    • Journal Title

      Journal of Experimental Botany

      Volume: 72 Issue: 6 Pages: 2083-2098

    • DOI

      10.1093/jxb/erab018

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Defects in the rice aconitase-encoding OsACO1 gene alter iron homeostasis2020

    • Author(s)
      Senoura Takeshi、Kobayashi Takanori、An Gynheung、Nakanishi Hiromi、Nishizawa Naoko K.
    • Journal Title

      Plant Molecular Biology

      Volume: 104 Issue: 6 Pages: 629-645

    • DOI

      10.1007/s11103-020-01065-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The bHLH protein OsIRO3 is critical for plant survival and iron (Fe) homeostasis in rice (Oryza sativa L.) under Fe-deficient conditions2020

    • Author(s)
      Wang Fan、Itai Reiko N.、Nozoye Tomoko、Kobayashi Takanori、Nishizawa Naoko K.、Nakanishi Hiromi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 66 Issue: 4 Pages: 579-592

    • DOI

      10.1080/00380768.2020.1783966

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Iron deficiency-inducible peptide-coding genes <i>OsIMA1</i> and <i>OsIMA2</i> positively regulate a major pathway of iron uptake and translocation in rice2020

    • Author(s)
      Kobayashi Takanori、Nagano Atsushi J、Nishizawa Naoko K
    • Journal Title

      Journal of Experimental Botany

      Volume: 72 Issue: 6 Pages: 2196-2211

    • DOI

      10.1093/jxb/eraa546

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Iron Recognition and Expressional Regulation of Iron-Related Genes in Plants~toward Production of Iron Deficiency-Tolerant and High Mineral Nutrition Rice~: How Plants Sense and Respond to Iron Deficiency2019

    • Author(s)
      KOBAYASHI Takanori
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 57 Issue: 12 Pages: 728-735

    • DOI

      10.1271/kagakutoseibutsu.57.728

    • NAID

      130007948468

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2019-12-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] OsbHLH058 and OsbHLH059 transcription factors positively regulate iron deficiency responses in rice2019

    • Author(s)
      Kobayashi Takanori、Ozu Asami、Kobayashi Subaru、An Gynheung、Jeon Jong-Seong、Nishizawa Naoko K.
    • Journal Title

      Plant Molecular Biology

      Volume: 101 Issue: 4-5 Pages: 471-486

    • DOI

      10.1007/s11103-019-00917-8

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Understanding the Complexity of Iron Sensing and Signaling Cascades in Plants2019

    • Author(s)
      Kobayashi Takanori
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 7 Pages: 1440-1446

    • DOI

      10.1093/pcp/pcz038

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Iron transport and its regulation in plants2019

    • Author(s)
      Kobayashi Takanori、Nozoye Tomoko、Nishizawa Naoko K.
    • Journal Title

      Free Radical Biology and Medicine

      Volume: 133 Pages: 11-20

    • DOI

      10.1016/j.freeradbiomed.2018.10.439

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hemerythrin E3 Ubiquitin Ligases as Negative Regulators of Iron Homeostasis in Plants2019

    • Author(s)
      Rodriguez-Celma Jorge、Chou Hsuan、Kobayashi Takanori、Long Terri A.、Balk Janneke
    • Journal Title

      Frontiers in Plant Science

      Volume: 10 Pages: 98-98

    • DOI

      10.3389/fpls.2019.00098

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イネ OsbZIP83 転写因子はユビキチンリガーゼ OsHRZ の制御下で鉄の体内輸送を促進する2022

    • Author(s)
      小林高範, 新川はるか, 永野惇, 西澤直子
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネのグルタレドキシンはユビキチンリガーゼ HRZ の制御下で鉄の利用を調節する2022

    • Author(s)
      新川はるか, 小林高範, 永野惇, 西澤直子
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Regulation of plant iron deficiency responses by iron-sensing HRZ/BTS ubiquitin ligases2021

    • Author(s)
      Takanori Kobayashi
    • Organizer
      The International Chemical Congress of Pacifichem Basin Societies 2021 (Pacifichem 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新規鉄肥料PDMAの効果検定とイネの鉄欠乏誘導性ペプチドOsIMAの機能解析2021

    • Author(s)
      小林高範
    • Organizer
      令和3年度 石川県立大学生物資源工学研究所 公開講座 2021年12月2日
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ユビキチンリガーゼ HRZ によるイネの鉄シグナリング2021

    • Author(s)
      小林高範
    • Organizer
      植物の栄養研究会 第6回研究交流会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ユビキチンリガーゼOsHRZのドメイン変異による鉄欠乏耐性・鉄蓄積イネの作製2021

    • Author(s)
      井原崇斗, 室田明星, 西澤直子, 小林高範, 中西啓仁
    • Organizer
      日本土壌肥料学会2021年度例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 鉄・亜鉛結合性ユビキチンリガーゼHRZによる植物の鉄欠乏応答と鉄感知2021

    • Author(s)
      小林高範
    • Organizer
      第62回日本植物生理学会年会シンポジウム「生命金属科学の新展開」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 植物の新規鉄欠乏応答制御因子の発見2020

    • Author(s)
      小林高範
    • Organizer
      令和2年度石川県立大学生物資源工学研究所公開講座
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] ユビキチンリガーゼHRZのゲノム編集による鉄欠乏耐性・鉄蓄積イネの作製2020

    • Author(s)
      室田明星, 井原崇斗, 西澤直子, 小林高範
    • Organizer
      日本土壌肥料学会中部支部2020年度例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 鉄の研究による植物の生産性と栄養価の向上2020

    • Author(s)
      小林高範
    • Organizer
      2020年度日本海イノベーション会議
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] イネの鉄欠乏誘導性ペプチドOsIMA1、OsIMA2は鉄欠乏応答を促進する2020

    • Author(s)
      小林高範, 永野惇, 西澤直子
    • Organizer
      日本土壌肥料学会2020年度岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Regulation of iron deficiency responses by glutaredoxins and bHLH transcription factors interacting with HRZ ubiquitin ligases2019

    • Author(s)
      Takanori Kobayashi, Asami Ozu, Subaru Kobayashi, Naoko K. Nishizawa
    • Organizer
      17th International Symposium on Rice Functional Genomics (ISRFG 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production and analysis of ubiquitin ligase OsHRZ rice mutants by CRISPR/Cas92019

    • Author(s)
      Akari Murota, Naoko K. Nishizawa, Takanori Kobayashi
    • Organizer
      17th International Symposium on Rice Functional Genomics (ISRFG 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of new iron deficiency-tolerant rice lines by combination of HRZ knockdown and introduction of mutational reconstructed ferric chelate reductase2019

    • Author(s)
      Keisuke Maeda, Rintaro Yuki, Takeshi Senoura, Hiroshi Masuda, May Sann Aung, Naoko K. Nishizawa, Takanori Kobayashi
    • Organizer
      17th International Symposium on Rice Functional Genomics (ISRFG 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネ転写因子OsbHLH058、OsbHLH059は鉄欠乏応答を正に制御する2019

    • Author(s)
      小林高範、小津亜沙美、小林昴、西澤直子
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] CRISPR/Cas9によるユビキチンリガーゼOsHRZ 変異イネの作出と解析2019

    • Author(s)
      室田明星・西澤直子・小林高範
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] HRZ ノックダウンと三価鉄還元酵素の組み合わせによる新規鉄欠乏耐性イネの創出2019

    • Author(s)
      前田慶介・結城麟太郎・瀬野浦武志・増田寛志・メイ サン アウン・西澤直子・小林高範
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulation of iron deficiency responses by HRZ ubiquitin ligases and their interacting partners2018

    • Author(s)
      Takanori Kobayashi
    • Organizer
      19th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] HRZ 結合性グルタレドキシンによるイネの鉄欠乏応答制御2018

    • Author(s)
      小林高範、西澤直子
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 鉄結合性ユビキチンリガーゼ HRZ による鉄栄養制御2018

    • Author(s)
      小林高範、メイサンアウン、増田寛志、瀬野浦武志、西澤直子
    • Organizer
      第11回北陸合同バイオシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Book] アルカリ性不良土壌での農業を可能にする環境調和型鉄吸収促進剤の開発 Vol. 79, No. 5, p. 386-387. バイオサイエンスとインダストリー2021

    • Author(s)
      小林高範, 鈴木基史, 難波康祐
    • Total Pages
      2
    • Publisher
      (一財)バイオインダストリー協会
    • Related Report
      2021 Annual Research Report
  • [Book] 「植物の鉄欠乏応答メカニズム、ミネラル含量の多い作物の創出」生命金属ダイナミクス 生体内における金属の挙動と制御 監修:城典嗣、津本浩平2021

    • Author(s)
      小林高範
    • Total Pages
      8
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2020 Annual Research Report
  • [Book] Iron biofortification: the gateway to overcoming hidden hunger; in The Future of Rice Demand: Quality Beyond Productivity, ed. de Oliveira, A.C., Pegoraro, C., Viana, V.E.2020

    • Author(s)
      Masuda, H., Aung, M.S., Kobayashi, T. and Nishizawa, N.K.
    • Total Pages
      29
    • Publisher
      Springer Inc.
    • Related Report
      2020 Annual Research Report
  • [Book] 「農業生産と環境保全、高ミネラル作物の開発へ向けた植物の鉄の研究」石川県立大学産学官ネットワークナウ2019

    • Author(s)
      小林高範
    • Total Pages
      5
    • Publisher
      石川県立大学産学官学術交流センター
    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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