|Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
|Outline of Final Research Achievements
We conducted in vitro analysis for putative substrates of rice HRZ ubiquitin ligases, including glutaredoxins (GRX), and bZIP and bHLH transcription factors. We found that GRXs and bZIP are subjected to HRZ-dependent degradation via the 26S proteasome pathway. We also produced and analyzed transgenic rice plants overexpressing or repressing these genes or iron deficiency-inducible IMA peptide genes. We found that GRXs and bZIP are novel downstream factors for HRZs, and are facilitating iron utilization in rice plants. We also found that bHLH and IMAs are novel positive regulators for rice iron deficiency responses.