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Functional analysis and application of terephthalate-degrading bacteria.

Research Project

Project/Area Number 18H02118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Nakajima-Kambe Toshiaki  筑波大学, 生命環境系, 教授 (80241777)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsテレフタル酸 / 微生物分解 / プラスチックリサイクル / 廃水処理 / 排水処理
Outline of Final Research Achievements

Terephthalic acid is used in various fields as a raw material for PET bottles, polyester fibers, and functional films for LCD panels. In the recycling of these products, liquid waste containing terephthalic acid is generated, and its treatment has become a problem. In this study, we identified relevant genes and analyzed the metabolic pathways of terephthalic acid-degrading bacteria (strain TB-97) based on the whole genome information, with the aim of producing useful substances (e.g. PDC) from terephthalic acid. We also determined the structure of a novel metal-recovery substance (siderophore) produced by this strain, and investigated the relationship between the microbial community structure and terephthalic acid degradation in the activated sludge tank from which this strain was isolated.

Academic Significance and Societal Importance of the Research Achievements

テレフタル酸廃棄物はそのまま回収しても市場価値が安く、精製及び再利用は採算が合わないため現在はコストをかけて焼却処分されている。本研究成果によってテレフタル酸含有廃液の高効率処理が行えるのみならず、高付加価値化も行うことができ、将来的にはPETのアップリサイクリングに貢献できる。またシデロフォアを用いたレアメタルの回収も同時に行える可能性がある。さらに、排水処理施設中の微生物叢の解析を行うことで、安定した処理を行うための管理・診断手法を提供できる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (6 results)

All 2021 2019 2018 Other

All Int'l Joint Research (1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results)

  • [Int'l Joint Research] Quaid-i-Azam University/PMAS UAAR University(パキスタン)

    • Related Report
      2021 Annual Research Report
  • [Presentation] Production Of 2-pyrone-4,6-dicarboxylic Acid From Terephthalic Acid In Pseudomonas sp. TB-972021

    • Author(s)
      K. Yamasaki, T. Kanamori, S. Nakajima, T. Uchibori, T. Nakajima-Kambe
    • Organizer
      World Microbe Forum
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production Of Useful Compounds From Waste Terephthalic Acid By Recombinant E.coli2021

    • Author(s)
      R. Komai, S. Nakajima, T. Kanamori, T. Uchibori, T. Nakajima-Kambe
    • Organizer
      World Microbe Forum
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pseudomonas sp. TB-97 株によるテレフタル酸からの有用物質生産2019

    • Author(s)
      金森 拓, 中島鈴佳, 岡村匡浩, 内堀孝博, 中島(神戸) 敏明
    • Organizer
      第71回日本生物工学会大会(岡山)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Terephthalate Degradation and Useful Substance Production by Bacteria.2019

    • Author(s)
      2)Nakajima Suzuka, Kanamori Taku, Okamura Masahiro, Uchibori Takahiro, Nakajima-Kambe Toshiaki
    • Organizer
      The 14th Asian Congress on Biotechnology (ACB2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pseudomonas sp. TB-98株によるテレフタル酸からのプロトカテク酸の生産2018

    • Author(s)
      中島(神戸)敏明, 金森拓, 中島鈴佳, 岡村匡浩, 内堀孝博
    • Organizer
      日本生物工学会第70回大会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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