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Mechanisms of CERK1 co-receptor-mediated plant defense and symbiotic responses

Research Project

Project/Area Number 18H02208
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionMeiji University

Principal Investigator

KAKU HANAE  明治大学, 農学部, 専任教授 (70409499)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords植物免疫 / キチン / LysM受容体 / 防御応答 / 共生応答 / 共受容体 / MAMP / LysM
Outline of Final Research Achievements

CERK1 is a LysM receptor kinase involved in the initiation of defense responses derived from carbohydrate ligand of bacterial and fungal cell walls. OsCERK1 is a bifunctional receptor, serving not only for the chitin/LPS-triggered defense response but also for arbuscular mycorrhizal (AM) symbiosis in rice, although the regulatory mechanism of these contradictory system is still unknown. In this study, we identified OsCERK2, which is structurally and functionally similar to OsCERK1. We found that the extra/intracellular region of CERK1 plays an important rule for the receptor complex formation to initiate the defense and symbiosis responses. We also indicated that several phosphorylation sites of AtCERK1 have critical function for the regulation of activation and downstream signaling.

Academic Significance and Societal Importance of the Research Achievements

世界の農作物は年間十数%が病害により失われ、この損失は数億人の食糧に匹敵する。しかし、植物はそう簡単に病気にはならない。植物には侵入する微生物のもつ特有な分子(MAMPs)を非自己として検出・排除する基礎的抵抗性(植物免疫)を持っている。本研究では植物免疫に関与するLysM受容体の受容・活性化機構の解析の成果は、植物の防御応答起動機構の解明だけでなく、“植物がLysM受容体を介して、どのように病原菌と共生菌の受け入れと相反する応答の切り替えを可能にするのか”など、生物学的に重要な多くの現象を理解する鍵になると考えられ、新規な病害抵抗性作

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (31 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (19 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] The National Research Council of Italy/University of Napoli Federico II(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Naples Federico II/National Research Council(イタリア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Cytoplasmic interaction of LysM receptors contributes to the formation of symbiotic receptor complex2020

    • Author(s)
      Maruya Suzuki, Ryota Numazaki, Tomomi Nakagawa, Naoto Shibuya and Hanae Kaku
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 3 Pages: 359362-359362

    • DOI

      10.5511/plantbiotechnology.20.0511a

    • NAID

      130007907445

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis cell culture for comparable physiological and genetic studies2020

    • Author(s)
      Maruyama Shingo、Shibuya Naoto、Kaku Hanae、Desaki Yoshitake
    • Journal Title

      Plant Signaling & Behavior

      Volume: 15 Issue: 8 Pages: 1781384-1781384

    • DOI

      10.1080/15592324.2020.1781384

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death2020

    • Author(s)
      Ye Wenxiu、Munemasa Shintaro、Shinya Tomonori、Wu Wei、Ma Tao、Lu Jiang、Kinoshita Toshinori、Kaku Hanae、Shibuya Naoto、Murata Yoshiyuki
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 34 Pages: 20932-20942

    • DOI

      10.1073/pnas.1922319117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Simultaneous visualization of callose deposition and plasma membrane for live cell imaging in plant2020

    • Author(s)
      Masaki Kohari, Naoto Shibuya, Hanae Kaku
    • Journal Title

      Plant Cell Reports

      Volume: 39 Issue: 11 Pages: 15171523-15171523

    • DOI

      10.1007/s00299-020-02580-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Handmade leaf cutter for efficient and reliable ROS assay2019

    • Author(s)
      Desaki Yoshitake、Shimada Hikaru、Takahashi Shohei、Sakurayama Chisa、Kawai Mika、Kaku Hanae、Shibuya Naoto
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 4 Pages: 275-278

    • DOI

      10.5511/plantbiotechnology.19.0921a

    • NAID

      130007776098

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-12-25
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] AtCERK1 Phosphorylation Site S493 Contributes to the Transphosphorylation of Downstream Components for Chitin-Induced Immune Signaling2019

    • Author(s)
      Suzuki Maruya、Yoshida Issei、Suto Kenkichi、Desaki Yoshitake、Shibuya Naoto、Kaku Hanae
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 8 Pages: 1804-1810

    • DOI

      10.1093/pcp/pcz096

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PUB4, a CERK1-Interacting Ubiquitin Ligase, Positively Regulates MAMP-Triggered Immunity in Arabidopsis2019

    • Author(s)
      Desaki Yoshitake、Takahashi Shohei、Sato Kenta、Maeda Kanako、Matsui Saki、Yoshimi Ikuya、Miura Takaki、Jumonji Jun-Ichi、Takeda Jun、Yashima Kohei、Kohari Masaki、Suenaga Takayoshi、Terada Hayato、Narisawa Tomoko、Shimizu Takeo、Yumoto Emi、Miyamoto Koji、Narusaka Mari、Narusaka Yoshihiro、Kaku Hanae、Shibuya Naoto
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 11 Pages: 2573

    • DOI

      10.1093/pcp/pcz151

    • URL

      https://pure.teikyo.jp/en/publications/74c2bfc9-f43b-4711-9b48-3bc7f8243467

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] MAMP-triggered plant immunity mediated by the LysM-receptor kinase CERK1,2019

    • Author(s)
      Yoshitake Desaki, Masaki Kohari, Naoto Shibuya, Hanae Kaku
    • Journal Title

      J. Gen. Plant Pathol.

      Volume: 85 Pages: 1-11

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AtCERK1 phosphorylation site S493 contributes to the transphosphorylation of downstream components for chitin-induced immune signaling2019

    • Author(s)
      Maruya Suzuki, Issei Yoshida, Kenkichi Suto, Yoshitake Desaki, Naoto Shibuya1 and Hanae Kaku
    • Journal Title

      Plant Cell Physiol.

      Volume: -

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 比較可能な生理学的、遺伝学的研究に資するシロイヌナズナ培養細胞の開発2021

    • Author(s)
      丸山真吾、高橋勇人、小川晃弘、渋谷直人、賀来華江、出崎能丈
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物免疫応答を評価する新規手法の開発2020

    • Author(s)
      丸山真吾、出崎能丈、島田日加瑠、高橋昌平、櫻山知彩、川井美佳、渋谷直人、賀来華江
    • Organizer
      第39回日本糖質学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Live cell imaging of chitin-induced callose deposition in Arabidopsis2019

    • Author(s)
      1.Masaki Kohari, Dexing Chen, Syuhei Ohashi, Naoto Shibuya, Hanae Kaku
    • Organizer
      International Society-Molecular Plant-Microbe Interactions XVIII congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PUB4, a novel CERK1 interactor, positively regulates chitin-induced immune signaling2019

    • Author(s)
      Yoshitake Desaki, Shohei Takahashi, Saki Matsui, Ikuya Yoshimi, Masaki Kohari, Emi Yumoto, Koji Miyamoto, Naoto Shibuya, Hanae Kaku
    • Organizer
      International Society-Molecular Plant-Microbe Interactions XVIII congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ネ LysM受容体 OsCERK1を介するLPS防御応答2019

    • Author(s)
      賀来華江、出崎能丈、香西雄介、南栄一、渋谷直人、西澤洋子
    • Organizer
      第38回日本糖質学会年会:日本糖質学会創設40周年記念大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ユビキチンリガーゼPUB4はキチンシグナル伝達を正に制御する2019

    • Author(s)
      出崎能丈、高橋昌平、松井紗樹、吉見育也、丸山真吾、湯本絵美、宮本皓司、渋谷直人、賀来華江
    • Organizer
      日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Handmade leaf cutter for efficient and reliable ROS assay2019

    • Author(s)
      Hanae Kaku, Yoshitake Desaki, Hikaru Shimada, Shohei Takahashi, Chisa Sakurayama, Mika Kawai, Naoto Shibuya
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物LysM受容体を介するシグナル応答2019

    • Author(s)
      賀来華江
    • Organizer
      環境および非自己応答機構から読み解く植物の巧みな生存戦略
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] キチン誘導性カロース蓄積の解析2019

    • Author(s)
      小針政輝、大橋周平、陳徳興、渋谷直人、賀来華江
    • Organizer
      環境および非自己応答機構から読み解く植物の巧みな生存戦略
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナCERK1相互作用因子PUB4の機能解析2019

    • Author(s)
      吉見育哉、大久保拓哉、三浦駿希、湯本絵美、宮本皓司、出崎能丈、渋谷直人、賀来華江
    • Organizer
      環境および非自己応答機構から読み解く植物の巧みな生存戦略
    • Related Report
      2018 Annual Research Report
  • [Presentation] 菌根菌共生応答に関わるイネLysM-RLKの解析2019

    • Author(s)
      長谷川駿、高橋牧、丸山真吾、宮田佳奈、澁谷直人、賀来華江
    • Organizer
      環境および非自己応答機構から読み解く植物の巧みな生存戦略
    • Related Report
      2018 Annual Research Report
  • [Presentation] Plant innate immune signaling mediated by LysM-receptors2019

    • Author(s)
      Hanae Kaku, Naoto Shibuya
    • Organizer
      Keystone Symposia-Innate Immune Receptors」.Roles in Immunology and Beyond-
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PUB4, a novel CERK1 interactor, positively regulate chitin-induced immune signaling2019

    • Author(s)
      Yoshitake Desaki, Shohei Takahashi, Saki Matsui, Ikuya Yoshimi, Masaki Kohari, Emi Yumoto, Koji Miyamoto, Naoto Shibuya, Hanae Kaku
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Interaction of cytoplasmic domains of LysM receptors is an important factor to determine the direction of downstream responses, defense or symbiosis2019

    • Author(s)
      Maruya Suzuki, Ryota Numazaki, Tomomi Nakagawa, Naoto Shibuya, Hanae Kaku
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] OsCERK1 regulates LPS-induced immune signaling in rice2018

    • Author(s)
      Yoshitake Desaki, Yusuke Kouzai, Yusuke Ninomiya, Ryosuke Iwase, Yumi Shimizu, Keito Seko, Antonio Molinaro, Eiichi Minami, Naoto Shibuya, Hanae Kaku, Yoko Nishizawa
    • Organizer
      INTERNATIONAL PLANT MOLECULAR BIOLOGY2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナCERK1相互作用因子PUB4はキチンシグナル伝達を正に制御する2018

    • Author(s)
      吉見育哉、大久保拓哉、三浦駿希、湯本絵美、丸山真吾、宮本皓司、出崎能丈、渋谷直人、賀来華江
    • Organizer
      第53回 植物感染生理談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キチンエリシター高感受性カロース蓄積評価系の構築2018

    • Author(s)
      小針政輝、大橋周平、陳徳興、渋谷直人、賀来華江
    • Organizer
      第53回 植物感染生理談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネLysM型受容体様キナーゼOsLysM-RLK10の機能解析2018

    • Author(s)
      長谷川駿、佐藤圭、神谷光太、白坂昴、安帝姫、出崎能丈、澁谷直人、賀来華江
    • Organizer
      第53回 植物感染生理談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネ原形質膜上に存在するLPS結合タンパク質の探索2018

    • Author(s)
      鈴木透、岩瀬良介、出崎能丈、渋谷直人、賀来華江
    • Organizer
      第53回 植物感染生理談話会
    • Related Report
      2018 Annual Research Report
  • [Book] Methods in Molecular Biology ”Lectin Purification and Analysis”2020

    • Author(s)
      Jun Hirabayashi (ed.) T. Shinya, N. Shibuya and H. Kaku
    • Publisher
      Humana Press
    • ISBN
      9781071604298
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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