|Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
|Outline of Final Research Achievements
Tobacco mosaic virus (TMV) has a single-stranded RNA genome in virions. The genomic RNA is translated in host cells to produce a 130-kDa protein and its read-through product, a 180-kDa protein (RNA polymerase). The 130-kDa protein co-translationally binds to a 5' terminal region of the genomic RNA and then recruits the 180-kDa protein to form a ribonucleoprotein complex named PMTC. This process is followed by genomic RNA replication. In this study, we found that insertion of a few nucleotides to the 5' terminus of the genomic RNA facilitates PMTC formation and that the 180-kDa protein is recruited to the PMTC via protein-protein interaction.