| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Outline of Final Research Achievements |
To clarify the molecular mechanisms of protein synthesis shutdown in anti-viral immune response of NPV-infected insect cells, we analyzed the mechanisms of rRNA degradation in Bombyx mori cells (BM-N cells) during abortive infection with heterologous nucleopolyhedrovirus (NPVs). In this research, we identified the minimal region of Ac-P143 responsible for rRNA degradation. By using the minimal region of Ac-P143, we have searched the cellular factors interacting with Ac-P143 and responsible for induction of rRNA degradation. By using fluorescence-labelled ribosomal proteins, we indicated that ribosomal proteins and/or ribosomes are degraded in the cytoplasm of infected cells.
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