• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanisms of the superior protective activities of intrinsically disordered proteins in cryo-preservable plant seeds

Research Project

Project/Area Number 18H02222
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39060:Conservation of biological resources-related
Research InstitutionShizuoka University

Principal Investigator

Hara Masakazu  静岡大学, 創造科学技術大学院, 教授 (10293614)

Co-Investigator(Kenkyū-buntansha) 今井 亮三  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, エグゼクティブリサーチャー (90291913)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords超低温保存 / タンパク質保護 / 天然変性タンパク質 / デハイドリン / 蛋白質保護 / 天然変性蛋白質
Outline of Final Research Achievements

We elucidated the mechanism of cryoprotective activity of dehydrin, an intrinsically disordered protein which is related to the preservation of plant seeds. We also purified another intrinsically disordered protein RVCaB from radish, found the cryoprotective activity, and identified the active segment. As a result, we found a common action mechanism between dehydrin and RVCaB, and proposed a new cryoprotective model which can complement the conventional protective models. It is suggested that there are diverse species of intrinsically disordered proteins in plants which can play an important role in their growth.

Academic Significance and Societal Importance of the Research Achievements

天然変性タンパク質は生物界に広く存在するが、その機能と生理学的な意義については多くの場合不明である。本研究は、植物天然変性タンパク質の超低温保護機能に着目し、そのメカニズムを解明した点で学術的な意義がある。植物由来の天然変性タンパク質は未利用素材であり、細胞や臓器の保存、冷凍並びに乾燥食品の製造、遺伝資源の保存など、様々な場面での応用が期待できる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (19 results)

All 2023 2022 2021 2020 2019 2018 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Remarks (3 results)

  • [Journal Article] Freeze-thaw-induced aggregation of bovine gamma globulin was efficiently inhibited by an intrinsically disordered plant protein dehydrin.2023

    • Author(s)
      Honami Osuda, Yuki Kimura, Masakazu Hara
    • Journal Title

      Food Hydrocolloids for Health

      Volume: 3 Pages: 100108-100108

    • DOI

      10.1016/j.fhfh.2022.100108

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibition of cryoaggregation of phospholipid liposomes by an Arabidopsis intrinsically disordered dehydrin and its K-segment2022

    • Author(s)
      Kimura Y, Ohkubo T, Shimizu K, Magata Y, Park EY, Hara M
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 211 Pages: 112286-112286

    • DOI

      10.1016/j.colsurfb.2021.112286

    • NAID

      120007180319

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An intrinsically disordered radish vacuolar calcium-binding protein (RVCaB) showed cryoprotective activity for lactate dehydrogenase with its hydrophobic region.2021

    • Author(s)
      Honami Osuda, Yui Sunano, Masakazu Hara
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 182 Pages: 1130-1137

    • DOI

      10.1016/j.ijbiomac.2021.04.056

    • NAID

      120007032775

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cryoprotective activity of Arabidopsis KS-type dehydrin depends on the hydrophobic amino acids of two active segments.2020

    • Author(s)
      Tomoka Yokoyama, Tomohiro Ohkubo, Keita Kamiya, Masakazu Hara
    • Journal Title

      Archives of Biochemistry and Biophysics

      Volume: 691 Pages: 108510-108510

    • DOI

      10.1016/j.abb.2020.108510

    • NAID

      120006872376

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] F-segments of Arabidopsis dehydrins show cryoprotective activities for lactate dehydrogenase depending on the hydrophobic residues2020

    • Author(s)
      Tomohiro Ohkubo, Ayuko Kameyama, Keita Kamiya, Mitsuru Kondo, Masakazu Hara
    • Journal Title

      Phytochemistry

      Volume: 173 Pages: 112300-112300

    • DOI

      10.1016/j.phytochem.2020.112300

    • NAID

      120006824776

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Functions and Applications of Intrinsically Disordered Proteins in Plant Seeds2023

    • Author(s)
      Masakazu Hara
    • Organizer
      ICONN2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dehydrins: Stress-Responsive Intrinsically Disordered Proteins in Plants2023

    • Author(s)
      Masakazu Hara
    • Organizer
      2023 4th International Conference of the Brain Korea21 FOUR
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] デハイドリンKセグメント配列による乳酸脱水素酵素凍結保護活性メカニズム2022

    • Author(s)
      大須田穂波、吉原有紗、原正和
    • Organizer
      第39回日本植物バイオテクノロジー 学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナデハイドリンによるリポソーム凍結保護作用に関する研究2022

    • Author(s)
      木村友紀、清水広介、間賀田泰寛、朴龍洙、原正和
    • Organizer
      第39回日本植物バイオテクノロジー 学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] デハイドリンおよび保存配列のリポソーム凍結凝集抑制に関する研究2021

    • Author(s)
      木村 友紀、大久保 智博、清水 広介、間賀田 泰寛、朴 龍洙、原 正和
    • Organizer
      第38回日本植物バイオテクノロジー(つくば)学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌナズナデハイドリンの乳酸脱水素酵素凍結保護活性における疎水性アミノ酸の役割2019

    • Author(s)
      横山 知佳、大久保 智博、神谷 慶太、原 正和
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] デハイドリンの凍結保護活性に対する疎水性アミノ酸の重要性2019

    • Author(s)
      大久保 智博、神谷 慶太、亀山 阿由子、原 正和
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Proteins in seeds for surviving under deep freeze conditions2019

    • Author(s)
      Masakazu Hara
    • Organizer
      2019 National Tsing Hua University-Shizuoka University Bilateral Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Adaptation of plants to extreme temperatures -Key proteins in seeds for surviving under deep freeze conditions-2019

    • Author(s)
      Masakazu HARA
    • Organizer
      Universiti Teknologi Malaysia, 2nd International Postgraduate Symposium in Biotechnology 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シロイヌナズナデハイドリンのnon-Kセグメントの酵素凍結保護活性における役割2019

    • Author(s)
      大久保智博、神谷慶太、亀山阿由子、原正和
    • Organizer
      日本農芸化学会2019年度大会[東京]
    • Related Report
      2018 Annual Research Report
  • [Presentation] デハイドリンCOR47の非Kセグメント領域の酵素凍結保護活性に関する研究2018

    • Author(s)
      大久保智博、神谷慶太、亀山阿由子、原正和
    • Organizer
      日本農芸化学会中部支部第183回例会[名古屋]
    • Related Report
      2018 Annual Research Report
  • [Remarks] 静岡大学教員データベース

    • URL

      https://tdb.shizuoka.ac.jp/rdb/public/Default2.aspx?id=10883&l=0

    • Related Report
      2022 Annual Research Report
  • [Remarks] 静岡大学農学部植物機能生理学研究室(原)

    • URL

      https://www.agr.shizuoka.ac.jp/abc/envplant/index.html

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.agr.shizuoka.ac.jp/abc/envplant/index.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi