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Sekizaisou, which produces red wood with high processibility and growth potential

Research Project

Project/Area Number 18H02250
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

KAJITA SHINYA  東京農工大学, (連合)農学研究科(研究院), 教授 (40323753)

Co-Investigator(Kenkyū-buntansha) 船田 良  東京農工大学, (連合)農学研究科(研究院), 教授 (20192734)
横山 岳  東京農工大学, (連合)農学研究科(研究院), 准教授 (20210635)
奥泉 久人  国立研究開発法人農業・食品産業技術総合研究機構, 遺伝資源研究センター, 主席研究員 (20370645)
光田 展隆  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (80450667)
池田 努  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (90334036)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywordsリグニン / クワ / 自然突然変異体 / 変異体 / 木材 / パルプ / リグノセルロース / 桑 / ゲノム
Outline of Final Research Achievements

We report a naturally occurring mulberry, Sekizaisou, which is deficient in the gene encoding CINNAMYL ALCOHOL DEHYDROGENASE (CAD) that catalyzes the last step in lignin monomer biosynthesis. Structural characterization of Sekizaisou wood revealed that substantial amounts of sinapaldehyde, one of the substrates for CAD, were integrally incorporated into its lignin. Metabolic profiling also revealed the strong accumulation of sinapaldehyde and products derived therefrom. We sequenced the genome of Sekizaisou, and annotated three putative CAD genes. The sequence of the homolog with the highest similarity to CAD genes that play a crucial role in lignin biosynthesis in other plant species exhibited a frame-shift mutation.

Academic Significance and Societal Importance of the Research Achievements

赤材桑(セキザイソウ)は、これまで世界で2例しか報告がなかったリグニンの分子構造を異にする樹木の自然突然変異体の3つ目の事例である。従来、遺伝子組換え技術によりリグニンの構造が変化した樹木が多く作出されてきたが、法的な規制のためにそのほとんどは野外での栽培が行われておらず、リグニンの改変がもたらす木材の被加工性の向上が、実用的な条件下でほとんど評価されていない。赤材桑は自然突然変異体であり、野外での栽培には制約がなく、長期にわたる育成が可能である。従って、この品種を有効に活用することで、リグニン構造が変化した木材の有用性を容易に検証可能で、木材の更なる有効活用方法の開発が加速するはずである。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (9 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Int'l Joint Research] ウィスコンシン大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ゲント大学(ベルギー)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase2021

    • Author(s)
      Ikeda Tsutomu、Takata Naoki、Sakamoto Shingo、Hu Shi、Nuoendagula、Hishiyama Shojiro、Mitsuda Nobutaka、Boerjan Wout、Ralph John、Kajita Shinya
    • Journal Title

      Holzforschung

      Volume: 0 Issue: 10 Pages: 968-977

    • DOI

      10.1515/hf-2021-0015

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 特異な材色を呈するクワの自然突然変異体「赤材桑」の飼料効率に関する検討2021

    • Author(s)
      持田裕司、岸雅嵩、小堀航平、武生魁世、近藤将人、胡石、野村義宏、新保博、梶田真也
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 90 Pages: 91-98

    • NAID

      130008090993

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection protocol for a mutant allele on the CINNAMYL ALCOHOL DEHYDROGENASE 1 locus of the Morus species and search trial for the allele in the natural mulberry population of Okushiri island2020

    • Author(s)
      Hachisuka H、Fukuda S、Iwase M、Inagaki S、Tomiyama H、Okuizumi H、Koyama A、Kajita S
    • Journal Title

      Lignin

      Volume: 1 Pages: 42-50

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 赤材桑のアルカリ蒸解廃液から回収されたリグニンの化学特性2021

    • Author(s)
      池田努、梶田真也
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] CAD遺伝子を欠損したクワの自然突然変異体から得られる木材と葉の潜在的な利用価値2021

    • Author(s)
      梶田真也
    • Organizer
      第66回リグニン討論会
    • Related Report
      2021 Annual Research Report
  • [Presentation] SEKIZAISOU: the first reported natural mutant with abnormal lignin structure in the world?2019

    • Author(s)
      SHINYA KAJITA
    • Organizer
      2nd International Symposium for Plant Cell Wall Engineering
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] SEKIZAISOU, the earliest discovered lignin mutant2018

    • Author(s)
      SHINYA KAJITA
    • Organizer
      Lignin, Gordon Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2018-04-23   Modified: 2024-12-25  

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