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Characterization and application of a novel cellulose-binding domain from brown rot fungi

Research Project

Project/Area Number 18H02252
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Yoshida Makoto  東京農工大学, (連合)農学研究科(研究院), 教授 (30447510)

Co-Investigator(Kenkyū-buntansha) 五十嵐 圭日子  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (80345181)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywordsセルロース結合ドメイン / 褐色腐朽菌 / CBM / 溶解性多糖モノオキシゲナーゼ
Outline of Final Research Achievements

The novel cellulose-binding domain (CBD) of the brown rot fungus Gloeophyllum trabeum, which is one of wood decay fungi in nature, was found to adsorb onto specifically crystalline cellulose with more than 10 times higher adsorption efficiency than previously known CBD. We also found that this novel CBD adsorbed on cellulose through a completely different mechanism with that of the previously known CBD. In addition, we found that this novel CBD could be used as a tool to monitor the distribution pattern of crystal regions in native cellulose.

Academic Significance and Societal Importance of the Research Achievements

セルロース結合ドメイン(CBD)は、様々なセルロース分解酵素に付加する構造的に独立した領域であり、そのセルロースに吸着する特性から、セルロースの構造的な特性の可視化や植物細胞壁構造の可視化、セルロース材料の改質などに用いることができるポテンシャルを有する。しかしながら、従来のCBDはセルロースへの特異性が低いという欠点を抱えている。本研究では、真菌類の一種が有する新たなCBDの機能解析により、このドメインが極めて高い特異性かつ高い親和性でセルロースに吸着することを見出した。これは、CBDを用いたセルロースの新たな解析法の提案につながると同時に、セルロースの新規改質技術の開発にも寄与する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(19 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (10 results)

  • [Int'l Joint Research] University of Massachusetts(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Norwegian University of Life Sciences(ノルウェー)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Massachusetts(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Nanostructural Analysis of Enzymatic and Non-enzymatic Brown Rot Fungal Deconstruction of the Lignocellulose Cell Wall2020

    • Author(s)
      Yuan Zhu, Nayomi Plaza, Yuka Kojima, Makoto Yoshida, Jiwei Zhang, Jody Jellison, Sai Venkatesh Pingali, Hugh O’Neill, and Barry Goodell
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 1389-1389

    • DOI

      10.3389/fmicb.2020.01389

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Role of Lytic Polysaccharide Monooxygenases in Wood Rotting Basidiomycetes2020

    • Author(s)
      Yuka Kojima, Aniko Vernai, Vincent G. H. Eijsink, Makoto Yoshida
    • Journal Title

      TIGG

      Volume: 32 Issue: 188 Pages: E135-E143

    • DOI

      10.4052/tigg.2020.7e

      10.4052/tigg.2020.7E

    • NAID

      130007879877

    • ISSN
      0915-7352, 1883-2113
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation.2020

    • Author(s)
      Kiwamu Umezawa, Mai Niikura, Yuka Kojima, Barry Goodell, Makoto Yoshida
    • Journal Title

      PLoS ONE

      Volume: 15

    • DOI

      10.1371/journal.pone.0243984

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multiple iron reduction by methoxylated phenolic lignin structures and the generation of reactive oxygen species by lignocellulose surfaces2019

    • Author(s)
      Yoshiaki Tamaru, Makoto Yoshida, Lindsay D Eltis, Barry Goodell
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 128 Pages: 340-346

    • DOI

      10.1016/j.ijbiomac.2019.01.149

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The crystal structure of the catalytic domain and the cytochrome b domain in a eukaryotic PQQ-dependent dehydrogenase2019

    • Author(s)
      Takeda, K., Ishida, T., Yoshida, M., Samejima, M., Hiroyuki Ohno, Igarashi, K., and Nakamura, N.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 85

    • DOI

      10.1128/aem.01692-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] X-ray diffraction and Fourier transform infrared spectroscopic analyses of wood blocks decayed by wood rotting fungi classified into Polyporales2019

    • Author(s)
      近藤 里沙子, 堀川 祥生, 半 智史, 安藤 恵介, 吉田 誠
    • Journal Title

      MOKUZAI HOZON (Wood Protection)

      Volume: 45 Issue: 6 Pages: 268-279

    • DOI

      10.5990/jwpa.45.268

    • NAID

      130007793472

    • ISSN
      0287-9255, 1884-0116
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeumにおいて見出された新規セルロース結合ドメインの点変異導入解析2021

    • Author(s)
      青木萌里、小嶋由香、吉田 誠、和田 昌久
    • Organizer
      第71回日本木材学会大会(東京大会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 新規結晶性セルロース結合モジュールの機能解析 およびセルロース評価への応用2021

    • Author(s)
      小嶋由香、田川聡美、吉田誠、砂川直輝、五十嵐圭日子
    • Organizer
      第71回日本木材学会大会(東京大会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeum由来GH10キシラナーゼに付加するセルロース結合ドメインの役割2021

    • Author(s)
      築田理華子、堀川祥生、吉田誠
    • Organizer
      第71回日本木材学会大会(東京大会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeum由来GH10キシラナーゼに付加するセルロース結合ドメインの生理的意義に関する研究2021

    • Author(s)
      築田理華子,堀川祥生、吉田誠
    • Organizer
      日本木材保存協会第37回年次大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeum 由来新規セルロース結合ドメインのセルロース結合メカニズムに関する研究2021

    • Author(s)
      青木萌里、小嶋由香、田川聡美、吉田誠、五十嵐圭日子、砂川直輝、和田昌久
    • Organizer
      日本木材保存協会第37回年次大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 新規結晶性セルロース結合モジュールを用いたセルロース結晶領域の蛍光標識2020

    • Author(s)
      小嶋由香、吉田 誠、砂川直輝、五十嵐圭日子
    • Organizer
      第70回日本木材学会大会(鳥取大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 異なる進化系統群に属する褐色腐朽菌を対象とした木材分解様式の比較解析2019

    • Author(s)
      近藤里沙子、堀川祥生、半 智史、安藤恵介、吉田 誠
    • Organizer
      日本木材保存協会第35回年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeum由来の新規結晶性セルロース結合モジュールの機能解析2019

    • Author(s)
      小嶋由香、吉田誠、砂川直輝、五十嵐圭日子
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異なる分岐群に属する褐色腐朽菌における腐朽様式の比較解析2018

    • Author(s)
      近藤里沙子、安藤恵介、半 智史、堀川祥生、吉田 誠
    • Organizer
      日本木材保存協会第34回年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 結晶性セルロースに特異的に結合する新規セルロース結合モジュールの機能解析2018

    • Author(s)
      小嶋由香、吉田誠、砂川直輝、五十嵐圭日子
    • Organizer
      セルラーゼ研究会第32回大会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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