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The mechanism of radial transportation of chemicals between the phloem and xylem in standing tree trunks determined by the novel imaging techniques

Research Project

Project/Area Number 18H02258
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionForest Research and Management Organization

Principal Investigator

Kuroda Katsushi  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (90399379)

Co-Investigator(Kenkyū-buntansha) 半 智史  東京農工大学, (連合)農学研究科(研究院), 准教授 (40627709)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsミネラル / 放射方向移動 / 蛍光トレーサー / 柔細胞 / セシウム / 立木凍結固定 / アポプラスト / シンプラスト / 蛍光物質
Outline of Final Research Achievements

We examined radial movement of minerals and chemicals in trees to understand the mechanism underlying their radial movement in the standing tree trunks. We revealed that artificially induced cesium as a tracer of minerals was moved radially in standing tree trunks through a combination of active transport by parenchyma cells and diffusion, and that artificially induced fluorescent materials as tracers of chemicals were moved in the radial direction while passing between symplast (the inner side of the plasma membrane of living cells) and apoplast (the space outside the plasma membrane including cell walls and dead cells).

Academic Significance and Societal Importance of the Research Achievements

幹に含まれるミネラルなどの成分は樹種ごとに異なっており、その違いが木材の色、耐久性、強度などの材質に影響する。ミネラルや糖などの成分を含む水は辺材の外側の組織や師部組織で樹幹軸方向(縦方向)に輸送されるため、心材など幹の中央部に含まれる成分は幹の外側から放射方向(半径方向)に動いて蓄積されると考えられているが、その移動の実態は実験として証明されていなかった。本研究では、できるだけ立木で起きている現象をそのまま解析する手法を用いて樹幹の放射方向(半径方向)の成分の移動の実態を細胞レベルで明らかにした。得られた成果は幹の材質の樹種間の違いを理解するための重要な手掛かりとなる。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(27 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (5 results) Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (11 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Int'l Joint Research] Natural Resources Institute Finland/Aalto University(フィンランド)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Natural Resources Institute Finland/Aalto University/Helsinki University(フィンランド)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] European Synchrotron Radiation Facility(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Natural Resources Institute Finland/Aalto University/Helsinki University(フィンランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] European Synchrotron Radiation Facility(フランス)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Cellular-level in planta analysis of radial movement of minerals in a konara oak (Quercus serrata Murray) trunk2022

    • Author(s)
      Kuroda Katsushi、Yamane Kenichi、Itoh Yuko
    • Journal Title

      Journal of Wood Science

      Volume: 68

    • DOI

      10.1186/s10086-022-02024-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] スギ樹幹半径方向のミネラル移動機構の解析―立木の中をそのまま見る工夫―2022

    • Author(s)
      黒田克史
    • Journal Title

      グリーンスピリッツ

      Volume: 17 Pages: 2-7

    • Related Report
      2020 Annual Research Report
  • [Journal Article] In Planta Analysis of the Radial Movement of Minerals from Inside to Outside in the Trunks of Standing Japanese Cedar (Cryptomeria japonica D. Don) Trees at the Cellular Level2021

    • Author(s)
      Kuroda Katsushi、Yamane Kenichi、Itoh Yuko
    • Journal Title

      Forests

      Volume: 12 Pages: 251-251

    • DOI

      10.3390/f12020251

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 樹木の心材形成機構の解明に向けて2021

    • Author(s)
      黒田克史
    • Journal Title

      季刊森林総研

      Volume: 54 Pages: 14-15

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] Radial Movement of Minerals in the Trunks of Standing Japanese Cedar (Cryptomeria Japonica D. Don) Trees in Summer by Tracer Analysis2020

    • Author(s)
      Kuroda Katsushi、Yamane Kenichi、Itoh Yuko
    • Journal Title

      Forests

      Volume: 11 Pages: 562-562

    • DOI

      10.3390/f11050562

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Localization of (+)-Catechin in Picea abies Phloem: Responses to Wounding and Fungal Inoculation2020

    • Author(s)
      T. Jyske, K. Kuroda, S. Keri, A. Pranovich, R. Linnakoski, N. Hayashi, D. Aoki and K. Fukushima
    • Journal Title

      Molecules

      Volume: 25 Pages: 2952-2952

    • DOI

      10.3390/molecules25122952

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualization and Localization of Submicron-Sized Ammonium Sulfate Particles on Needles of Japanese Larch (Larix kaempferi) and Japanese Cedar (Cryptomeria japonica) and Leaves of Japanese Beech (Fagus crenata) and Japanese Chinquapin (Castanopsis sieboldii) after Artificial Exposure2019

    • Author(s)
      Yamane Kenichi、Nakaba Satoshi、Yamaguchi Masahiro、Kuroda Katsushi、Sano Yuzou、Lenggoro I. Wuled、Izuta Takeshi、Funada Ryo
    • Journal Title

      Forests

      Volume: 10 Pages: 1151-1151

    • DOI

      10.3390/f10121151

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Water potential gradient between sapwood and heartwood as a driving force in water accumulation in wetwood in conifers2019

    • Author(s)
      Nakada Ryogo、Okada Naoki、Nakai Takahisa、Kuroda Katsushi、Nagai Satoshi
    • Journal Title

      Wood Science and Technology

      Volume: 53 Pages: 407-424

    • DOI

      10.1007/s00226-019-01081-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 立木の幹内部の水・元素分布を細胞レベルで解析する-立木凍結固定伐採法とクライオSEM/EDX法-2019

    • Author(s)
      黒田克史
    • Journal Title

      木材工業

      Volume: 74 Pages: 76-80

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular level in planta analysis of radial movement of artificially injected caesium in Cryptomeria japonica xylem2018

    • Author(s)
      Kuroda Katsushi、Yamane Kenichi、Itoh Yuko
    • Journal Title

      Trees

      Volume: 32 Pages: 1505-1517

    • DOI

      10.1007/s00468-018-1729-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] スギ木部放射組織における物質移動経路の可視化手法の検討2021

    • Author(s)
      松田泰至、黒田克史、船田良、半智史
    • Organizer
      日本木材学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] クライオSEM/EDXによるスギ辺材に注入したセシウムの樹幹外側から内側への放射方向の移動解析2020

    • Author(s)
      黒田克史、山根健一、伊藤優子
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 蛍光トレーサーを用いたスギ放射組織における物質移動経路の解析2020

    • Author(s)
      松田泰至、黒田克史、船田 良、半 智史
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カキノキ木部の黒色部位における着色物質の移動経路および木部繊維細胞壁への沈着2020

    • Author(s)
      岩見佳奈、乃万 了、伴 琢也、荒川 泉、松下泰幸、船田 良、半 智史
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 樹木の木部放射組織における物質移動の蛍光トレーサーを用いた解析2019

    • Author(s)
      半 智史、松田泰至、船田 良、黒田克史
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] スギ樹幹辺材のミネラル放射方向移動における木部柔細胞の寄与2019

    • Author(s)
      黒田克史、山根健一
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光トレーサーを用いた木部放射組織における物質移動の可視化2019

    • Author(s)
      半智史、松田泰至、船田良、黒田克史
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光トレーサーを用いたケヤキの放射組織を経由する物質移動の観察2019

    • Author(s)
      松田泰至、黒田克史、船田良、半智史
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カキノキ辺材部の黒色化に伴う木部柔細胞の内容物の変化2019

    • Author(s)
      岩見佳奈、乃万了、伴琢也、松下泰幸、船田良、半智史
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ニセアカシアの心材形成過程における心材成分と細胞内容物の蛍光スペクトルイメージングによる同時観察2019

    • Author(s)
      太田萌、船田良、半智史
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cytological Changes in Ray Parenchyma Cells during Artificially Induced Cell Death in Cryptomeria japonica2018

    • Author(s)
      Satoshi Nakaba, Izumi Arakawa, Hikaru Morimoto, Yusuke Yamagishi, Ryogo Nakada, Nobumasa Bito, Takanori Imai, Ryo Funada
    • Organizer
      2018 SWST/JWRS International Convention
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 太い幹の内部でミネラルが半径方向に移動する実態を立木の実験で解明

    • URL

      http://www.ffpri.affrc.go.jp/research/saizensen/2021/20210511-02.html

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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