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Molecular mechanisms of sex differentiation and sex-reversal in medaka complex, Oryzias sakaizumii, as a new model species for aquaculture

Research Project

Project/Area Number 18H02281
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionUniversity of Shizuoka

Principal Investigator

Kobayashi Tohru  静岡県立大学, 食品栄養科学部, 教授 (30221972)

Co-Investigator(Kenkyū-buntansha) 明正 大純  静岡県立大学, 食品栄養科学部, 助教 (00781808)
小林 靖尚  近畿大学, 農学部, 准教授 (10643786)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords性分化・性転換 / メダカ / gsdf / dmrt1 / amh / 性分化関連遺伝子 / クエ / 性分化・転換 / キタノメダカ / 性分化 / 性転換 / ミナミメダカ / 性決定 / 水産育種モデル / ナイルティラピア
Outline of Final Research Achievements

The sex differentiation cascade of Oryzias sakaizumii is very similar to that of Nile tilapia, a useful fisheries species for aquaculture, unlike O. latipes. This study is conducting research using this species as a new model by comparing it to sex differentiation and masculinization in tilapia and masculinization in Grouper. In this study, we examined the molecular mechanisms of gonadal differentiation and sex change from sex determination. Thus we demonstrate the existence of an unknown testis differentiation pathway as the primary pathway for sex transdifferentiation to the testis.

Academic Significance and Societal Importance of the Research Achievements

本研究では、キタノメダカの性分化、性転換の分子機構を水産有用種であるティラピア、クエの性転換機構と比較検討した結果、雄への性転換には、Dmrt1は必要であるが、その誘導や分化には新規の精巣分化経路が存在することが明らかとなった。このことは、新規の分化経路が、Gsdfの発現が精巣分化誘導と相関しない種やDmrt1の発現が精巣分化と相関しない種の精巣分化(性転換を含)においても共通である可能性を強く示唆するものであり、水産増殖における性統御に資する技術開発を前進させる成果である

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (26 results)

All 2022 2021 2020 2019 2018 Other

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 7 results) Presentation (10 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Preself-Feeding Medaka Fry Provides a Suitable Screening System for in Vivo Assessment of Thyroid Hormone-Disrupting Potential2022

    • Author(s)
      Myosho, T., Ishibashi, A., Fujimoto, S., Miyagawa, S., Iguchi, T., Kobayashi, T.
    • Journal Title

      Environmental Science & Technology

      Volume: - Issue: 10 Pages: 6479-6490

    • DOI

      10.1021/acs.est.1c06729

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Control of gonadal maturation and sex in grouper.2022

    • Author(s)
      Kobayashi, Y.
    • Journal Title

      Aquaculture and Fishers

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of synthetic androgen treatment on the gonad of juvenile longtooth grouper (Epinephelus bruneus).2021

    • Author(s)
      Kobayashi Y, Morishita T, Chuda H.
    • Journal Title

      Int Aquat Res.

      Volume: 113 Pages: 163-171

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of synthetic sex steroid hormone exposures on gonadal sex differentiation and dynamics of a male-related gene, Gonadal soma-derived factor (Gsdf) and an estrogen up-regulated gene, Choriogenine-H (ChgH) gene expression in the euryhaline Javafish medaka, Oryzias javanicus, based on genetic sexes2021

    • Author(s)
      Myosho T, Hattori M, Yamamoto J, Toda M, Okamura T, Onishi Y, Takehana Y, Kobayashi T.
    • Journal Title

      Chemosphere

      Volume: 274 Pages: 129893-129893

    • DOI

      10.1016/j.chemosphere.2021.129893

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of G protein‐coupled estrogen receptors in Japanese medaka, Oryzias latipes2020

    • Author(s)
      Miyaoku Kaori、Ogino Yukiko、Lange Anke、Ono Ayaka、Kobayashi Tohru、Ihara Masaru、Tanaka Hiroaki、Toyota Kenji、Akashi Hiroshi、Yamagishi Genki、Sato Tomomi、Tyler Charles R.、Iguchi Taisen、Miyagawa Shinichi
    • Journal Title

      Journal of Applied Toxicology

      Volume: - Issue: 9 Pages: 1390-1399

    • DOI

      10.1002/jat.4130

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Viviparous stingrays avoid contamination of the embryonic environment through faecal accumulation mechanisms2020

    • Author(s)
      Tomita Taketeru、Nakamura Masaru、Kobayashi Yasuhisa、Yoshinaka Atsushi、Murakumo Kiyomi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1-7

    • DOI

      10.1038/s41598-020-64271-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shift of the Vegetal Pole Area of Full-Grown Oocytes Toward the Ovulatory Site of the Ovary in the Medaka Fish, Oryzias latipes (Beloniformes: Adrianichthyidae)2019

    • Author(s)
      Takashi Iwamatsu, Shoji Oda, Hirokuni Kobayashi, Lynne R. Parenti, Yasuhisa Kobayashi
    • Journal Title

      The Biological Bulletin

      Volume: in press

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ハタ科魚類の性分化と性転換に関する形態学的および生理学的研究2019

    • Author(s)
      村田良介, 野津了, 小林靖尚, 中村將
    • Journal Title

      日本水産学雑誌

      Volume: in press

    • NAID

      130007884617

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inter- and intraspecific variation in sex hormone induced sex-reversal in medakas, Oryzias latipes and Oryzias sakaizumi2019

    • Author(s)
      Myosho, T., Sato, T., Nishiyama, H., Watanabe, A., Yamamoto, J., Okamura, T., Onishi, Y., Fujimaki, R., Hamaguchi, S., Sakaizumi, M. and Kobayashi, T
    • Journal Title

      Zoological Science

      Volume: 36 Issue: 5 Pages: 425-431

    • DOI

      10.2108/zs180194

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyperosmotic tolerance of adult fish and early embryos are determined by discrete, single loci in the genus Oryzias2018

    • Author(s)
      Myosho Taijun、Takahashi Hideya、Yoshida Kento、Sato Tadashi、Hamaguchi Satoshi、Sakamoto Tatsuya、Sakaizumi Mitsuru
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 6897-6897

    • DOI

      10.1038/s41598-018-24621-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 長崎県平戸産ミナミメダカ個体群におけるDmyに次ぐ第2の性決定遺伝子GsdfneoYの獲得機構と第3の性決定遺伝子の探索2021

    • Author(s)
      明正 大純、藤本慎吾、小林 亨
    • Organizer
      日本動物学会第92回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アンドロゲン投与がクエ幼魚の生殖腺へ及ぼす影響2021

    • Author(s)
      小林靖尚
    • Organizer
      第45回 日本比較内分泌学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 新旧性決定遺伝子Dmy とGsdfneoY が共存する長崎県平戸産野生ミナミメダカ個体群.2020

    • Author(s)
      明正 大純、藤本慎吾、小林 亨
    • Organizer
      第67回日本生態学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 長崎県平戸産ミナミメダカ個体群におけるRetrotransposonの挿入による新規性決定遺伝子GsdfneoYへの進化.2020

    • Author(s)
      明正 大純、藤本慎吾、小林 亨
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ミナミメダカとキタノメダカにおける性ホルモン曝露による性転換誘導の種間・種内多様性とGsdfの関係2019

    • Author(s)
      明正 大純、渡邉 明帆、小林 亨
    • Organizer
      公益社団法人日本動物学会第90回大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミナミメダカのエストロゲンによる性転換誘導における感受性の系統差はGsdfの発現制御と相関する2019

    • Author(s)
      明正 大純、西山 ひろか、渡邉 明帆、小林 亨
    • Organizer
      第22回環境ホルモン学会研究発表会 (東京)
    • Related Report
      2019 Annual Research Report
  • [Presentation] プロゲスチン曝露によるメダカの性転換 III2019

    • Author(s)
      渡邉 明帆、明正 大純、小林 亨
    • Organizer
      第22回環境ホルモン学会研究発表会 (東京)
    • Related Report
      2019 Annual Research Report
  • [Presentation] ナイルティラピアの生殖腺性分化・転換過程におけるAmhの発現動態2018

    • Author(s)
      小林 亨
    • Organizer
      日本動物学会第89回 (札幌)
    • Related Report
      2018 Annual Research Report
  • [Presentation] キタノメダカHNI-II系統における性決定初期に高発現するDmrt1の種内共通性の探索とその機能解析2018

    • Author(s)
      明正 大純, 佐藤 忠, 酒泉 満, 小林 亨
    • Organizer
      日本動物学会第89回 (札幌)
    • Related Report
      2018 Annual Research Report
  • [Presentation] EE2曝露によるメダカエストロゲン応答遺伝子の反応性2018

    • Author(s)
      松谷 優佳里、明正 大純、小林 亨
    • Organizer
      環境ホルモン学会第21回研究発表会 (東京)
    • Related Report
      2018 Annual Research Report
  • [Book] 軟骨魚類の繁殖と性分化. In魚類の性決定・性分化・性転換-これまでとこれから (e-水産学シリーズ). 第7章. 145-166.2020

    • Author(s)
      小林靖尚、野津了、中村將
    • Total Pages
      21
    • Publisher
      恒星社厚生閣
    • Related Report
      2020 Annual Research Report
  • [Book] 魚類の性転換-多様な性転換から見えてきた共通性. In 魚類の性決定・性分化・性転換-これまでとこれから (e-水産学シリーズ).第8章. 167-190.2020

    • Author(s)
      野津了、小林靖尚、中村將
    • Total Pages
      23
    • Publisher
      恒星社厚生閣
    • Related Report
      2020 Annual Research Report
  • [Book] Sex Differentiation, Sex Change, and Sex Control in Groupers. In: Sex Control in Aquaculture.2019

    • Author(s)
      Masaru Nakamura, Yasuhisa Kobayashi
    • Publisher
      Wiley-Blackwell
    • ISBN
      9781119127260
    • Related Report
      2018 Annual Research Report
  • [Book] Variety of Sex Change in Tropical Fish. In: Reproductive and Developmental Strategies.2018

    • Author(s)
      Yasuhisa Kobayashi, Ryo Nozu, Ryo Horiguchi, Masaru Nakamura
    • Publisher
      Springer
    • ISBN
      9784431566090
    • Related Report
      2018 Annual Research Report
  • [Book] 日本動物学会(編)動物学の百科事典. 4章-12 解析手法としての遺伝学2018

    • Author(s)
      明正 大純
    • Total Pages
      808
    • Publisher
      丸善出版
    • ISBN
      9784621303092
    • Related Report
      2018 Annual Research Report
  • [Remarks] メダカ孵化仔魚を用いた化学物質の甲状腺ホルモン撹乱活性の新規検出法を確立

    • URL

      https://www.u-shizuoka-ken.ac.jp/news/20220511/

    • Related Report
      2021 Annual Research Report

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Published: 2018-04-23   Modified: 2024-01-30  

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