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Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst

Research Project

Project/Area Number 18H02321
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionHokkaido University

Principal Investigator

Kawahara Manabu  北海道大学, 農学研究院, 准教授 (70468700)

Co-Investigator(Kenkyū-buntansha) 唄 花子  北海道大学, 農学研究院, 助教 (60775443)
永野 昌志  北里大学, 獣医学部, 教授 (70312402)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywordsウシ / 胚盤胞期胚 / 個体発生 / 栄養膜細胞 / ゲノミック評価 / 初期胚 / 細胞分化 / 育種 / 内部細胞塊 / 栄養外胚葉 / 初期胚発生
Outline of Final Research Achievements

The blastocyst consists of two types of cells, the inner cell mass (ICM) and the trophectoderm (TE), which develop into an embryo proper and a large part of the placenta, respectively. We have shown that, since bovine ICM cells can regenerate TE, they are capable of forming both the embryo and placenta. To test developmental ability of TE-regenerated embryos, we then transferred the regenerated blastocysts to recipient females. After the embryo-transfer, to their surprise, one of the four cows became pregnant and a female calf was naturally born with an apparently normal placenta. Our study suggests that we can remove and use a large part of TE for genetic testing to breed cattle with improved qualities. Also, further studies could reveal the mechanism of cell fate decision in mammals and its differences between species.

Academic Significance and Societal Importance of the Research Achievements

今回の研究から、ウシ胚盤胞期胚のICMは完全なTE再生機能を保持していることが証明されたので、TEの大部分を利用して遺伝子検査に供した上で個体生産できる可能性が示された。さらに、ウシ胚のTEのYAP1局在はマウス胚TEとは異なって動的に局在様式が変化していることが判明し、これによりマウス胚などと比べて緩やかに細胞分化が進行するウシ胚の発生が調節されていると推察された。本研究を足掛かりに生理現象の中でのYAP1細胞内局在の多様な制御機構の理解が深まることが期待され、さらに研究を進めることで、哺乳類の細胞運命決定のメカニズムや種による違いが明らかになるかもしれない。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (20 results)

All 2021 2020 2019 2018 Other

All Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (7 results) (of which Int'l Joint Research: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Requirement for expression of WW domain containing transcription regulator 1 in bovine trophectoderm development2021

    • Author(s)
      Saito Shun、Yamamura Shota、Kohri Nanami、Bai Hanako、Takahashi Masashi、Kawahara Manabu
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 555 Pages: 140-146

    • DOI

      10.1016/j.bbrc.2021.03.112

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipopolysaccharide and lipoteichoic acid influence milk production ability via different early responses in bovine mammary epithelial cells.2021

    • Author(s)
      Tsugami Y, Wakasa H, Kawahara M, Nishimura T, Kobayashi K
    • Journal Title

      Experimental cell Research

      Volume: 15 Issue: 2 Pages: 112472-112472

    • DOI

      10.1016/j.yexcr.2021.112472

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adverse effects of LPS on membrane proteins in lactating bovine mammary epithelial cells.2021

    • Author(s)
      Tsugami Y, Wakasa H, Kawahara M, Watanabe A, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Cell and Tissue Research

      Volume: ahead of print Issue: 2 Pages: 435-448

    • DOI

      10.1007/s00441-020-03344-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of summer heat stress on gene expression in bovine uterine endometrial tissues2020

    • Author(s)
      Bai Hanako, Ukita Haruka, Kawahara Manabu, Mitani Tomohiro, Furukawa Eri, Yanagawa Yojiro, Yabuuchi Naoto, Kim Heejin, Takahashi Masashi
    • Journal Title

      Animal Science Journal

      Volume: 91 Issue: 1 Pages: 1-9

    • DOI

      10.1111/asj.13474

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Yes-associated protein 1 translocation through actin cytoskeleton organization in trophectoderm cells2020

    • Author(s)
      Yamamura Shota, Goda Nanami, Akizawa Hiroki, Kohri Nanami, Balboula Ahmed Z, Kobayashi Ken, Bai Hanako, Takahashi Masashi, Kawahara Manabu
    • Journal Title

      Developmental Biology

      Volume: 468 Issue: 1-2 Pages: 14-25

    • DOI

      10.1016/j.ydbio.2020.09.004

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Vitrification-induced activation of lysosomal cathepsin B perturbs spindle assembly checkpoint function in mouse oocytes2020

    • Author(s)
      Balboula Ahmed Z、Schindler Karen、Kotani Tomoya、Kawahara Manabu、Takahashi Masashi
    • Journal Title

      Molecular Human Reproduction

      Volume: 26 Issue: 9 Pages: 689-701

    • DOI

      10.1093/molehr/gaaa051

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The role of RHOA signaling in trophectoderm cell-fate decision in cattle2020

    • Author(s)
      Kohri Nanam, Akizawa Hiroki, Iisaka Sakie, Bai Hanako, Takahashi Masashi, Kawahara Manabu
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 528 Issue: 4 Pages: 713-718

    • DOI

      10.1016/j.bbrc.2020.05.210

    • NAID

      120007126837

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst2019

    • Author(s)
      Kohri Nanami、Akizawa Hiroki、Iisaka Sakie、Bai Hanako、Yanagawa Yojiro、Takahashi Masashi、Komatsu Masaya、Kawai Masahito、Nagano Masashi、Kawahara Manabu
    • Journal Title

      Journal of Biological Chemistry

      Volume: 294 Issue: 50 Pages: 19209-19223

    • DOI

      10.1074/jbc.ra119.010746

    • NAID

      120006940625

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of use of conventional and sexed semen on the conception rate in heifers: A comparison study2019

    • Author(s)
      Oikawa Kohei、Yamazaki Takeshi、Yamaguchi Satoshi、Abe Hayato、Bai Hanako、Takahashi Masashi、Kawahara Manabu
    • Journal Title

      Theriogenology

      Volume: 135 Pages: 33-37

    • DOI

      10.1016/j.theriogenology.2019.06.012

    • NAID

      120006882075

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overgrowth of mice generated from postovulatory aged oocyte spindles.2019

    • Author(s)
      Shiina K, Komatsu M, Yokoi F, Bai H, Takahashi T, Kawahara M.
    • Journal Title

      FASEB BioAdvances

      Volume: 印刷中 Issue: 7 Pages: 1-11

    • DOI

      10.1096/fba.2019-00005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Significance of CCN2 expression in bovine preimplantation development2018

    • Author(s)
      Akizawa Hiroki、Yanagawa Yojiro、Nagano Masashi、Bai Hanako、Takahashi Masashi、Kawahara Manabu
    • Journal Title

      Animal Science Journal

      Volume: 90 Issue: 1 Pages: 49-54

    • DOI

      10.1111/asj.13126

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effect of EAS on HSP70 related progesterone synthesis in bovine cumulusgranulosa cells.2021

    • Author(s)
      Khoi Thieu HO, Kohei HOMMA, Jun TAKANARI, Tomohiro MITANI, Hanako BAI, Manabu KAWAHARA, Kim Khang Thi NGUYEN, Masashi TAKAHASHI.
    • Organizer
      日本畜産学会、九州大学(福岡県)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Effect of zona pellucida on early development and gene expression in mouse embryos.2021

    • Author(s)
      Weihong FAN, Hanako BAI, Manabu KAWAHARA, Masashi TAKAHASHI.
    • Organizer
      日本畜産学会、九州大学(福岡県)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Species-specific dynamics of mitochondrial content within an embryo during preimplantation development in mice and cattle.2020

    • Author(s)
      Komatsu M., Yokoi F., Imai S., Yamane M., Bai H., Takahashi M., and Kawahara M.
    • Organizer
      Fertility Conference and Exhibition 2020, Edinburgh (UK).
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hippo経路標的分子TAZ 遺伝子の発現抑制がウシ初期胚に及ぼす影響.2018

    • Author(s)
      山村頌太, 郡七海, 秋沢宏紀, 唄花子, 高橋昌志, 川原学.
    • Organizer
      日本繁殖生物学会 (長野県上田市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウシ子宮内膜上皮細胞を用いた暑熱負荷培養およびインターフェロン・タウ応答性の検証.2018

    • Author(s)
      小松正明, 鈴木惇文, 国井宏樹, 川原学, 木村康二, 高橋昌志, 唄花子.
    • Organizer
      日本繁殖生物学会 (長野県上田市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乳牛の分娩前後における末梢血単核球のストレスおよび免疫関連因子の解析.2018

    • Author(s)
      唄花子, Shabur Md Abdus TALUKDER, 国井宏樹, 伊藤月乃, 川原学, 高橋昌志.
    • Organizer
      日本繁殖生物学会 (長野県上田市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 性選別精液による乳牛人工授精の受胎率に及ぼす環境要因の影響.2018

    • Author(s)
      及川康平, 山崎武志, 山口諭, 阿部隼人, 唄花子, 高橋昌志, 川原学.
    • Organizer
      日本繁殖生物学会 (長野県上田市)
    • Related Report
      2018 Annual Research Report
  • [Remarks] 北海道大学農学部 遺伝繁殖学研究室

    • URL

      http://lab.agr.hokudai.ac.jp/anim/breed/index.html

    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 内部細胞塊から腔再形成胚を作製する方法および腔再形成胚を用いたウシ個体の作出方法2019

    • Inventor(s)
      川原学、郡七海、高橋昌志、唄花子
    • Industrial Property Rights Holder
      川原学、郡七海、高橋昌志、唄花子
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-172732
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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