Methane mitigation from ruminants by wide range application of alkylpenol-containing plants

• Project/Area Number: 18H02322
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Hokkaido University
• Principal Investigator: 小林 泰男 北海道大学, 農学研究院, 教授 (50153648)
• Co-Investigator(Kenkyū-buntansha): 鈴木 裕 北海道大学, 農学研究院, 助教 (10793846) ; 小池 聡 北海道大学, 農学研究院, 准教授 (90431353)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: アルキルフェノール / ギンナン果肉 / 糞便 / メタン / 微生物相 / カシュー殻液 / スラリー / 温室効果 / ルーメン / 排泄物 / メタン低減 / カシューナッツ殻液 / 温暖化

Outline of Annual Research Achievements

前2年間で東南アジアの代表的反芻家畜であるタイ在来牛と沼沢水牛へメタン低減素材・カシュー殻液を含有する飼料製剤を給与し、その機能評価を実施した。その結果、ルーメンおよび糞便からのメタン削減に対する高いインパクトが期待できることがわかった。
最終年度においては、日本国内においてウシ排泄糞便からのメタン低減を目標として、初年度に実施したギンナン果肉の直接添加の影響について、微生物相の変遷を主眼に検討した。すなわち、北海道大学のホルスタイン種泌乳牛の糞便へ、ギンナン果肉を添加・放置し、発酵産物（測定済み）と微生物相への影響を評価した。
糞便のメタン生成量は放置30日以降180日まで全期間においてギンナン果肉添加時に減少した。ギンナン果肉の添加によって全期間を通して酢酸モル比が低下し、プロピオン酸モル比は上昇した。これらの変化は糞便菌叢の変化と連動していた。すなわち、メタン古細菌 (特にMethanocorpusculum)に加えRuminococcaceaeやClostridiaceae の減少が生じ、一方でBacteroidaceae や Porphyloromonadaceae の増加が観察された。減少した菌群はメタンやメタンの基質となる水素生成に関与するものが含まれ、増加した菌群にはプロピオン酸生成に関連するものが含まれる。
以上の結果は、糞便へのギンナン果肉の直接添加が微生物相を変え、メタン低減を導きうることを示唆するが、その効果は比較的長期間期待できるもの、と思われた。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Kasetsart University(タイ)
• [Int'l Joint Research] Faculty of Agriculture/Kasetsart University(タイ)
• [Journal Article] Microbial community structure of the bovine rumen as affected by feeding cashew nut shell liquid, a methane‐inhibiting and propionate‐enhancing agent. (2021)
  • Author(s): Su, C., Shinkai, T., Miyazawa, N., Mitsumori, M., Enishi, O., Nagashima, K., Koike,S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92
  • DOI: 10.1111/asj.13503
  • NAID: 120007181808
  • Peer Reviewed
• [Journal Article] Feeding cashew nut shell liquid decreases methane production from feces by altering fecal bacterial and archaeal communities in Thai local ruminants. (2021)
  • Author(s): Tamori, K., Matsunaga, B., Boonsaen, P., Khongpradit, A., Sawanon, S.,Nagashima, K., Koike, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92
  • DOI: 10.1111/asj.13569
  • NAID: 120007181804
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Growth and morphologic response of rumen methanogenic archaea and bacteria to cashew nut shell liquid and its alkylphenol components. (2021)
  • Author(s): Wakai, M., Hayashi, S., Chiba, Y., Koike, S., Nagashima, K., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92
  • DOI: 10.1111/asj.13598
  • Peer Reviewed
• [Journal Article] Addition of ginkgo fruit to cattle feces and slurry suppresses methane production by altering the microbial community structure. (2021)
  • Author(s): Shintani, R., Oh, S., Suzuki, Y., Koike, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 92
  • DOI: 10.1111/asj.13620
  • Peer Reviewed
• [Journal Article] Cashew nut shell liquid potentially mitigates methane emission from the feces of Thai native ruminant livestock by modifying fecal microbiota. (2021)
  • Author(s): Narabe, C., Kamiyama, S., Saito, M., Boonsaen, P., Khongpradit, A., Sawanon, S., Suzuki , Y., Koike , S, Kobayashi , Y.
  • Journal Title: Animal Science Journal Volume: 92
  • DOI: 10.1111/asj.13614
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 環境調和をみすえる家畜栄養学の展開：温暖化ガスの低減に向けて (2020)
  • Author(s): 小林泰男
  • Journal Title: Nihon Chikusan Gakkaiho Volume: 91 Issue: 3 Pages: 304-306
  • DOI: 10.2508/chikusan.91.304
  • NAID: 130007922113
  • ISSN: 1346-907X, 1880-8255
• [Journal Article] Effect of ruminal administration of soy sauce oil on rumen fermentation, milk production and blood parameters in dairy cows. (2020)
  • Author(s): Konno, D., Takahashi, M., Osaka, I,, Orihashi, T., Sakai, K., Sera, K., Obara, Y., Kobayashi, Y.
  • Journal Title: Asian-Australasian Journal of Animal Sciences Volume: 33 Pages: 1779-1786
  • DOI: 10.5713/ajas.19.0617
  • Peer Reviewed / Open Access
• [Journal Article] Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle (2020)
  • Author(s): Khongpradit, A., Boonsaen, O., Homwong, N., Suzuki, Y., Koike, S., Sawanon, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 91
  • DOI: 10.1111/asj.13459
  • NAID: 120007151359
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The bio‐surfactant mannosylerythritol lipid acts as a selective antibacterial agent to modulate rumen fermentation (2020)
  • Author(s): Okuhira, K., Koike, S., Ito, S., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 91
  • DOI: 10.1111/asj.13464
  • Peer Reviewed
• [Journal Article] Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter (2019)
  • Author(s): Yamano, H., Ichimura, Y., Sawabe, Y., Koike, S., Suzuk, Y., Kobayashi, Y.
  • Journal Title: Animal Science Journal Volume: 90 Pages: 790-798
  • DOI: 10.1111/asj.13203
  • NAID: 120006847762
  • Peer Reviewed
• [Journal Article] Effect of cashew nut shell liquid feeding on fermentation and microbiota in the rumen of Thai native cattle and swamp buffaloes (2019)
  • Author(s): Konda, S., Onodera, R., Kanchanasatit, E., Boonsaen, P., Sawanon, S., Nagashima, K., Suzuki, Y., Koike, S., Kobayashi, Y.
  • Journal Title: Livestock Science Volume: 226 Pages: 99-106
  • DOI: 10.1016/j.livsci.2019.06.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Selection of plant oil as a supplemental energy source by monitoring rumen profiles and its dietary application in Thai crossbred beef cattle (2019)
  • Author(s): Matsuba, K., Padlom, A., Khongpradit, A., Boonsaen, P., Thirawong, P., Sawanon, S., Suzuki, Y., Koike, S., Kobayashi, Y.
  • Journal Title: Asian-Australasian Journal of Animal Science Volume: 32
  • DOI: 10.5713/ajas.18.0946
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rumen responses to dietary supplementation with cashew nut shell liquid and its cessation in sheep (2018)
  • Author(s): Kang S, Suzuki R, Suzuki Y, Koike S, Nagashima K, Kobayashi Y
  • Journal Title: Animal Science Journal Volume: 89 Pages: 1549-1555
  • DOI: 10.1111/asj.13100
  • NAID: 120006734168
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Rumen methane suppression toward low carbon ruminant production. (2021)
  • Author(s): Kobayashi, Y.
  • Organizer: International webinar on rumen methane suppression. Online operated by Seoul National University. September 17
  • Int'l Joint Research / Invited
• [Presentation] Mitigation of methane from cattle through controlling gut microbiota and Fermentation. (2021)
  • Author(s): Kobayashi, Y.
  • Organizer: Materials Research Meeting 2021. Pacifico Yokohama. Decmber 13.
  • Int'l Joint Research / Invited
• [Presentation] Effect of early roughage feeding on growth and hindgut environment of pre-weaned calves (2021)
  • Author(s): Heshan Kodithuwakku・大和田尚・平野和夫・三浦広卓・鈴木裕・小林泰男・小池聡
  • Organizer: 日本畜産学会第128回大会
• [Presentation] 他菌の発酵産物がルーメン細菌Selenomonas ruminantium の運動性と増殖性に及ぼす影響 (2021)
  • Author(s): 宮崎黎・鈴木裕・小林泰男・小池聡
  • Organizer: 日本畜産学会第128回大会
• [Presentation] Recent challenges for mitigation of methane emission from ruminants (2020)
  • Author(s): Yasuo Kobayashi
  • Organizer: Korean Society of Rumen Function Studies
  • Int'l Joint Research / Invited
• [Presentation] 消化管環境の制御による家畜生産性の向上と代謝障害の予防 (2019)
  • Author(s): 小林泰男
  • Organizer: 家畜感染症学会
  • Invited
• [Presentation] 第一胃微生物からみたウシの疾病と栄養制御 (2019)
  • Author(s): 小林泰男
  • Organizer: 大動物臨床研究会
  • Invited
• [Presentation] ルーメン微生物研究グループからの発表 (2018)
  • Author(s): 小林泰男
  • Organizer: 第2回畜産分野での温室効果ガス排出削減に向けた研究ネットワーク会合
  • Invited
• [Book] 畜産学概論 (2021)
  • Author(s): 小林泰男 他9名
  • Total Pages: 184
  • Publisher: 朝倉書店
  • ISBN: 9784254450316
• [Remarks] 研究テーマ１：動物機能栄養学
  • URL: https://anim-func-nutr.agr.hokudai.ac.jp/introduction/theme01/
• [Remarks]
  • URL: https://anim-func-nutr.agr.hokudai.ac.jp/
• [Remarks] 動物機能栄養学研究室
  • URL: https://www.agr.hokudai.ac.jp/r/lab/animal-function-and-nutrition?from=us