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Methane mitigation from ruminants by wide range application of alkylpenol-containing plants

Research Project

Project/Area Number 18H02322
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionHokkaido University

Principal Investigator

小林 泰男  北海道大学, 農学研究院, 教授 (50153648)

Co-Investigator(Kenkyū-buntansha) 鈴木 裕  北海道大学, 農学研究院, 助教 (10793846)
小池 聡  北海道大学, 農学研究院, 准教授 (90431353)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywordsアルキルフェノール / ギンナン果肉 / 糞便 / メタン / 微生物相 / カシュー殻液 / スラリー / 温室効果 / ルーメン / 排泄物 / メタン低減 / カシューナッツ殻液 / 温暖化
Outline of Annual Research Achievements

前2年間で東南アジアの代表的反芻家畜であるタイ在来牛と沼沢水牛へメタン低減素材・カシュー殻液を含有する飼料製剤を給与し、その機能評価を実施した。その結果、ルーメンおよび糞便からのメタン削減に対する高いインパクトが期待できることがわかった。
最終年度においては、日本国内においてウシ排泄糞便からのメタン低減を目標として、初年度に実施したギンナン果肉の直接添加の影響について、微生物相の変遷を主眼に検討した。すなわち、北海道大学のホルスタイン種泌乳牛の糞便へ、ギンナン果肉を添加・放置し、発酵産物(測定済み)と微生物相への影響を評価した。
糞便のメタン生成量は放置30日以降180日まで全期間においてギンナン果肉添加時に減少した。ギンナン果肉の添加によって全期間を通して酢酸モル比が低下し、プロピオン酸モル比は上昇した。これらの変化は糞便菌叢の変化と連動していた。すなわち、メタン古細菌 (特にMethanocorpusculum)に加えRuminococcaceaeやClostridiaceae の減少が生じ、一方でBacteroidaceae や Porphyloromonadaceae の増加が観察された。減少した菌群はメタンやメタンの基質となる水素生成に関与するものが含まれ、増加した菌群にはプロピオン酸生成に関連するものが含まれる。
以上の結果は、糞便へのギンナン果肉の直接添加が微生物相を変え、メタン低減を導きうることを示唆するが、その効果は比較的長期間期待できるもの、と思われた。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Report

(4 results)
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(27 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (13 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 12 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 6 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] Kasetsart University(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Faculty of Agriculture/Kasetsart University(タイ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Microbial community structure of the bovine rumen as affected by feeding cashew nut shell liquid, a methane‐inhibiting and propionate‐enhancing agent.2021

    • Author(s)
      Su, C., Shinkai, T., Miyazawa, N., Mitsumori, M., Enishi, O., Nagashima, K., Koike,S., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 92

    • DOI

      10.1111/asj.13503

    • NAID

      120007181808

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Feeding cashew nut shell liquid decreases methane production from feces by altering fecal bacterial and archaeal communities in Thai local ruminants.2021

    • Author(s)
      Tamori, K., Matsunaga, B., Boonsaen, P., Khongpradit, A., Sawanon, S.,Nagashima, K., Koike, S., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 92

    • DOI

      10.1111/asj.13569

    • NAID

      120007181804

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Growth and morphologic response of rumen methanogenic archaea and bacteria to cashew nut shell liquid and its alkylphenol components.2021

    • Author(s)
      Wakai, M., Hayashi, S., Chiba, Y., Koike, S., Nagashima, K., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 92

    • DOI

      10.1111/asj.13598

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Addition of ginkgo fruit to cattle feces and slurry suppresses methane production by altering the microbial community structure.2021

    • Author(s)
      Shintani, R., Oh, S., Suzuki, Y., Koike, S., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 92

    • DOI

      10.1111/asj.13620

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cashew nut shell liquid potentially mitigates methane emission from the feces of Thai native ruminant livestock by modifying fecal microbiota.2021

    • Author(s)
      Narabe, C., Kamiyama, S., Saito, M., Boonsaen, P., Khongpradit, A., Sawanon, S., Suzuki , Y., Koike , S, Kobayashi , Y.
    • Journal Title

      Animal Science Journal

      Volume: 92

    • DOI

      10.1111/asj.13614

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 環境調和をみすえる家畜栄養学の展開:温暖化ガスの低減に向けて2020

    • Author(s)
      小林泰男
    • Journal Title

      Nihon Chikusan Gakkaiho

      Volume: 91 Issue: 3 Pages: 304-306

    • DOI

      10.2508/chikusan.91.304

    • NAID

      130007922113

    • ISSN
      1346-907X, 1880-8255
    • Related Report
      2020 Annual Research Report
  • [Journal Article] Effect of ruminal administration of soy sauce oil on rumen fermentation, milk production and blood parameters in dairy cows.2020

    • Author(s)
      Konno, D., Takahashi, M., Osaka, I,, Orihashi, T., Sakai, K., Sera, K., Obara, Y., Kobayashi, Y.
    • Journal Title

      Asian-Australasian Journal of Animal Sciences

      Volume: 33 Pages: 1779-1786

    • DOI

      10.5713/ajas.19.0617

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of pineapple stem starch feeding on rumen microbial fermentation, blood lipid profile, and growth performance of fattening cattle2020

    • Author(s)
      Khongpradit, A., Boonsaen, O., Homwong, N., Suzuki, Y., Koike, S., Sawanon, S., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 91

    • DOI

      10.1111/asj.13459

    • NAID

      120007151359

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The bio‐surfactant mannosylerythritol lipid acts as a selective antibacterial agent to modulate rumen fermentation2020

    • Author(s)
      Okuhira, K., Koike, S., Ito, S., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 91

    • DOI

      10.1111/asj.13464

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter2019

    • Author(s)
      Yamano, H., Ichimura, Y., Sawabe, Y., Koike, S., Suzuk, Y., Kobayashi, Y.
    • Journal Title

      Animal Science Journal

      Volume: 90 Pages: 790-798

    • DOI

      10.1111/asj.13203

    • NAID

      120006847762

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of cashew nut shell liquid feeding on fermentation and microbiota in the rumen of Thai native cattle and swamp buffaloes2019

    • Author(s)
      Konda, S., Onodera, R., Kanchanasatit, E., Boonsaen, P., Sawanon, S., Nagashima, K., Suzuki, Y., Koike, S., Kobayashi, Y.
    • Journal Title

      Livestock Science

      Volume: 226 Pages: 99-106

    • DOI

      10.1016/j.livsci.2019.06.011

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Selection of plant oil as a supplemental energy source by monitoring rumen profiles and its dietary application in Thai crossbred beef cattle2019

    • Author(s)
      Matsuba, K., Padlom, A., Khongpradit, A., Boonsaen, P., Thirawong, P., Sawanon, S., Suzuki, Y., Koike, S., Kobayashi, Y.
    • Journal Title

      Asian-Australasian Journal of Animal Science

      Volume: 32

    • DOI

      10.5713/ajas.18.0946

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rumen responses to dietary supplementation with cashew nut shell liquid and its cessation in sheep2018

    • Author(s)
      Kang S, Suzuki R, Suzuki Y, Koike S, Nagashima K, Kobayashi Y
    • Journal Title

      Animal Science Journal

      Volume: 89 Pages: 1549-1555

    • DOI

      10.1111/asj.13100

    • NAID

      120006734168

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Rumen methane suppression toward low carbon ruminant production.2021

    • Author(s)
      Kobayashi, Y.
    • Organizer
      International webinar on rumen methane suppression. Online operated by Seoul National University. September 17
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mitigation of methane from cattle through controlling gut microbiota and Fermentation.2021

    • Author(s)
      Kobayashi, Y.
    • Organizer
      Materials Research Meeting 2021. Pacifico Yokohama. Decmber 13.
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of early roughage feeding on growth and hindgut environment of pre-weaned calves2021

    • Author(s)
      Heshan Kodithuwakku・大和田尚・平野和夫・三浦広卓・鈴木裕・小林泰男・小池聡
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 他菌の発酵産物がルーメン細菌Selenomonas ruminantium の運動性と増殖性に及ぼす影響2021

    • Author(s)
      宮崎黎・鈴木裕・小林泰男・小池聡
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Recent challenges for mitigation of methane emission from ruminants2020

    • Author(s)
      Yasuo Kobayashi
    • Organizer
      Korean Society of Rumen Function Studies
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 消化管環境の制御による家畜生産性の向上と代謝障害の予防2019

    • Author(s)
      小林泰男
    • Organizer
      家畜感染症学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 第一胃微生物からみたウシの疾病と栄養制御2019

    • Author(s)
      小林泰男
    • Organizer
      大動物臨床研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ルーメン微生物研究グループからの発表2018

    • Author(s)
      小林泰男
    • Organizer
      第2回畜産分野での温室効果ガス排出削減に向けた研究ネットワーク会合
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Book] 畜産学概論2021

    • Author(s)
      小林泰男 他9名
    • Total Pages
      184
    • Publisher
      朝倉書店
    • ISBN
      9784254450316
    • Related Report
      2020 Annual Research Report
  • [Remarks] 研究テーマ1:動物機能栄養学

    • URL

      https://anim-func-nutr.agr.hokudai.ac.jp/introduction/theme01/

    • Related Report
      2021 Annual Research Report
  • [Remarks]

    • URL

      https://anim-func-nutr.agr.hokudai.ac.jp/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 動物機能栄養学研究室

    • URL

      https://www.agr.hokudai.ac.jp/r/lab/animal-function-and-nutrition?from=us

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-12-28  

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