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Analysis of the life cycle of Babesia in ticks and development of transmission-blocking vaccines

Research Project

Project/Area Number 18H02336
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

玄 学南  帯広畜産大学, 原虫病研究センター, 教授 (10292096)

Co-Investigator(Kenkyū-buntansha) 白藤 梨可  帯広畜産大学, 原虫病研究センター, 助教 (00549909)
正谷 達謄  岐阜大学, 応用生物科学部, 准教授 (70614072)
山岸 潤也  北海道大学, 人獣共通感染症リサーチセンター, 准教授 (80535328)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Granted (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsバベシア / マダニ / ライフサイクル / 伝播阻止ワクチン / 犬バベシア症 / 生活環 / 媒介機構 / Babesia gibsoni / 有性生殖期誘導 / 有性生殖期マーカー分子 / CCp / Babesai gibsoni / Babesia microti / 伝播阻止 / ワクチン / 伝搬阻止
Outline of Annual Research Achievements

バベシア原虫はマダニによって媒介される病原体であり、種々の動物に重篤な貧血を引き起こし、家畜・ペット産業に重大な損害をもたらしている。本研究は、実験室レベルで全ライフサイクル解析が可能なバベシア原虫とフタトゲチマダ二を材料とし、マダニ体内におけるバベシア原虫発育の分子基盤解明と、それに基づくバベシア原虫伝播阻止ワクチンの開発を目指す。当該年度に得られた成果は、下記の通りである。1)in vitro培養系におけるB. gibsoniの有性生殖期虫体の誘導方法(キサンツレン酸の添加による誘導法)を確立した。2)有性生殖期マーカー分子とされるCCp1、CCp2、CCp3の発現が確認された。3)CCp1、CCp2、CCp3遺伝子をクローニングし、組換えタンパク質を作製した。4)組換えCCp1、CCp2、CCp3をマウスに免疫し、特異抗体を作製した。5)蛍光抗体法により、CCp1、CCp2、CCp3の特異抗体は、有性生殖期虫体と特異的反応を示すことを確認した。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

当初の計画通り進展し、所期の結果が得られたため。

Strategy for Future Research Activity

有性生殖期特異的発現分子に対する抗体のマダニ体内虫体発育への抑制効果を検討する。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(14 results)

All 2020 2019 2018

All Journal Article (7 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Subolesin vaccination inhibits blood feeding and reproduction of Haemaphysalis longicornis in rabbits2020

    • Author(s)
      Lee et al.
    • Journal Title

      Parasit Vectors

      Volume: 13 Pages: 478-478

    • DOI

      10.1186/s13071-020-04359-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti2020

    • Author(s)
      Zheng Weiqing、Umemiya-Shirafuji Rika、Zhang Qian、Okado Kiyoshi、Adjou Moumouni Paul Franck、Suzuki Hiroshi、Chen Haiying、Liu Mingming、Xuan Xuenan
    • Journal Title

      Front Physiol

      Volume: 11 Pages: 502-502

    • DOI

      10.3389/fphys.2020.00502

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of Haemaphysalis longicornis Genes Differentially Expressed in Response to Babesia microti Infection2020

    • Author(s)
      Zheng Weiqing、Umemiya-Shirafuji Rika、Chen Shengen、Okado Kiyoshi、Adjou Moumouni Paul Franck、Suzuki Hiroshi、Yang Shu、Liu Mingming、Xuan Xuenan
    • Journal Title

      Pahtogens

      Volume: 9 Pages: 378-378

    • DOI

      10.3390/pathogens9050378

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Babesia microti confers macrophage-babed cross-protective immunity against murine malaria2020

    • Author(s)
      Efstratiou et al.
    • Journal Title

      Front Cell Infect Microbiol

      Volume: 10 Pages: 193-193

    • DOI

      10.3389/fcimb.2020.00193

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ransient Transfection of the Zoonotic Parasite Babesia microti2020

    • Author(s)
      Liu M, Ji S, Rizk MA, Adjou Moumouni PF, Galon EM, Li J, Li Y, Zheng W, Benedicto B, Tumwebaze MA, Asada M, Xuan X
    • Journal Title

      Pathogens

      Volume: 9(2)

    • DOI

      10.3390/pathogens9020108

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inhibitory effects of the phytohormone inhibitors fluridone and inabenfide against Babesia gibsoni in vitro2019

    • Author(s)
      Liu et al.
    • Journal Title

      Veterinary Parasitology

      Volume: 265 Pages: 19-23

    • DOI

      10.1016/j.vetpar.2018.11.018

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni2018

    • Author(s)
      Liu Mingming、Adjou Moumouni Paul Franck、Asada Masahito、Hakimi Hassan、Masatani Tatsunori、Vudriko Patrick、Lee Seung-Hun、Kawazu Shin-ichiro、Yamagishi Junya、Xuan Xuenan
    • Journal Title

      Parasite Vectors

      Volume: 11 Pages: 260-260

    • DOI

      10.1186/s13071-018-2853-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Genome editing in the human Babesia parasite Babesia microti using the CRISPR-Cas9 system2019

    • Author(s)
      Liu et al.
    • Organizer
      第65回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification and expression of subolesin in Haemaphysalis longicornis2019

    • Author(s)
      Lee et al.
    • Organizer
      27th Conference of the World Association for the Advancement of Veterinary Parasitology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome editing of Babesia microti using the CRISPR-Cas9 system2019

    • Author(s)
      Liu et al.
    • Organizer
      27th Conference of the World Association for the Advancement of Veterinary Parasitology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inhibitory effects of the phytohormone inhibitors fluridone and inabenfide against Babesia gibsoni in vitro2018

    • Author(s)
      Liu et al.
    • Organizer
      第88回日本寄生虫学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Establish of a stable transfection system for genetic manipulation of Babesia gibsoni2018

    • Author(s)
      Liu et al.
    • Organizer
      第161回日本獣医学会(招待講演)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Identification and expression of subolesin in Haemaphysalis longicornis2018

    • Author(s)
      Lee et al.
    • Organizer
      第88回日本寄生虫学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Prevalence and molecular characterization of tick-borne pathogens amongst sheep and goats in Sudan2018

    • Author(s)
      Lee et al.
    • Organizer
      14th International Congress of Parasitology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-12-28  

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