Analysis of the life cycle of Babesia in ticks and development of transmission-blocking vaccines

• Project/Area Number: 18H02336
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: 玄 学南 帯広畜産大学, 原虫病研究センター, 教授 (10292096)
• Co-Investigator(Kenkyū-buntansha): 白藤 梨可 帯広畜産大学, 原虫病研究センター, 助教 (00549909) ; 正谷 達謄 岐阜大学, 応用生物科学部, 准教授 (70614072) ; 山岸 潤也 北海道大学, 人獣共通感染症リサーチセンター, 准教授 (80535328)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Granted (Fiscal Year 2021)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: バベシア / マダニ / ライフサイクル / 伝播阻止ワクチン / 犬バベシア症 / 生活環 / 媒介機構 / Babesai gibsoni / Babesia microti / 伝播阻止 / ワクチン / 伝搬阻止 / Babesia gibsoni

Outline of Annual Research Achievements

バベシア原虫はマダニによって媒介される病原体であり、種々の動物に重篤な貧血を引き起こし、家畜・ペット産業に重大な損害をもたらしている。本研究は、実験室レベルで全ライフサイクルの解析が可能なバベシア原虫（Babesia gibsoni/Babesia microti）とフタトゲチマダニ（Haemaphysalis longicornis）を材料とし、マダニ体内におけるバベシア原虫発育の分子基盤の解明と、それに基づくバベシア原虫伝播阻止ワクチンの開発を目指す。当該年度に得られた成果は、下記の通りである。１）人工吸血法によりB. gibsoni-GFPのマダニへの感染実験系を改良し、その効果を上げた。２）マダニ体内におけるB. gibsoni-GFP虫体の発育を観察し、また、マダニ体内特異分子の特定もできた。３）B. microti-GFPを作製した。４）B. microtiのマダニへの感染系を確立した。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

当初の計画通りに進展し、所期の結果が得られたため。

Strategy for Future Research Activity

今後、マダニ体内におけるバベシア虫体特異分子の探索を繰り返す必要がある。

Research Products

• [Journal Article] ransient Transfection of the Zoonotic Parasite Babesia microti (2020)
  • Author(s): Liu M, Ji S, Rizk MA, Adjou Moumouni PF, Galon EM, Li J, Li Y, Zheng W, Benedicto B, Tumwebaze MA, Asada M, Xuan X
  • Journal Title: Pathogens Volume: 9(2)
  • DOI: 10.3390/pathogens9020108
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Inhibitory effects of the phytohormone inhibitors fluridone and inabenfide against Babesia gibsoni in vitro (2019)
  • Author(s): Liu et al.
  • Journal Title: Veterinary Parasitology Volume: 265 Pages: 19-23
  • DOI: 10.1016/j.vetpar.2018.11.018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni (2018)
  • Author(s): Liu Mingming、Adjou Moumouni Paul Franck、Asada Masahito、Hakimi Hassan、Masatani Tatsunori、Vudriko Patrick、Lee Seung-Hun、Kawazu Shin-ichiro、Yamagishi Junya、Xuan Xuenan
  • Journal Title: Parasite Vectors Volume: 11 Pages: 260-260
  • DOI: 10.1186/s13071-018-2853-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Genome editing in the human Babesia parasite Babesia microti using the CRISPR-Cas9 system (2019)
  • Author(s): Liu et al.
  • Organizer: 第65回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
• [Presentation] Identification and expression of subolesin in Haemaphysalis longicornis (2019)
  • Author(s): Lee et al.
  • Organizer: 27th Conference of the World Association for the Advancement of Veterinary Parasitology
  • Int'l Joint Research
• [Presentation] Genome editing of Babesia microti using the CRISPR-Cas9 system (2019)
  • Author(s): Liu et al.
  • Organizer: 27th Conference of the World Association for the Advancement of Veterinary Parasitology
  • Int'l Joint Research
• [Presentation] Inhibitory effects of the phytohormone inhibitors fluridone and inabenfide against Babesia gibsoni in vitro (2018)
  • Author(s): Liu et al.
  • Organizer: 第88回日本寄生虫学会
• [Presentation] Establish of a stable transfection system for genetic manipulation of Babesia gibsoni (2018)
  • Author(s): Liu et al.
  • Organizer: 第161回日本獣医学会(招待講演)
  • Invited
• [Presentation] Identification and expression of subolesin in Haemaphysalis longicornis (2018)
  • Author(s): Lee et al.
  • Organizer: 第88回日本寄生虫学会
• [Presentation] Prevalence and molecular characterization of tick-borne pathogens amongst sheep and goats in Sudan (2018)
  • Author(s): Lee et al.
  • Organizer: 14th International Congress of Parasitology
  • Int'l Joint Research