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Mechanism for establishing connections between homologous chromosomes in mammalian meiosis

Research Project

Project/Area Number 18H02353
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42030:Animal life science-related
Research InstitutionKobe University

Principal Investigator

Lee Jibak  神戸大学, 農学研究科, 准教授 (50372660)

Co-Investigator(Kenkyū-buntansha) 平谷 伊智朗  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (40583753)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords減数分裂 / コヒーシン / 精母細胞 / 相同染色体 / 対合
Outline of Final Research Achievements

In this project, we made two lines of knock-in mice that express meiosis-specific cohesin subunit, RAD21L or REC8, tagged with 3×FLAG. We determined the expression ratio of RAD21L and REC8 and the absolute amount of cohesins in a mouse spermatocyte by western blot analysis with anti-3×FLAG antibody. Furthermore, we sought to identify RAD21L- and REC8-associated proteins by MS/MS analysis following immunoprecipitation using testes extracts from wild-type (control) and knock-in mice. As a result, besides the proteins that are known to associate with mitotic cohesin, a novel protein whose interaction with cohesin has never known was found in the immunoprecipitates.

Academic Significance and Societal Importance of the Research Achievements

哺乳類の減数分裂におけるコヒーシンの解析は,免疫組織染色法による局在やノックアウトマウスを利用した生体内機能解析が進む一方で,細胞内の発現量や個々のコヒーシンの生化学的解析は遅れていた。本研究では,マウス精母細胞でコヒーシンの発現量を初めて明らかにした。卵母細胞では,コヒーシン量の減少が母体の加齢に伴う染色体異常の原因の一つと考えられているので,本研究はそれを今後調べる上で重要な基礎的知見と今後の研究資料を提供している。また,減数分裂で発現する種類の異なるコヒーシンがどのような分子と相互作用しているかを明らかにすることにより,役割分担の理解も進むと思われる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (4 results)

All 2021 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Is age-related increase of chromosome segregation errors in mammalian oocytes caused by cohesin deterioration?2019

    • Author(s)
      Lee J.
    • Journal Title

      Reprod Med Biol

      Volume: 19 Issue: 1 Pages: 32-41

    • DOI

      10.1002/rmb2.12299

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] マウス精母細胞における減数分裂特異的コヒーシンの解析2021

    • Author(s)
      李智博,魏興強,堀居拓郎,畑田出穂
    • Organizer
      第51回精子研究会・神戸大学先端融合研究環研究プロジェクト(開拓09-神戸大学発次世代農資源生産システム)共催講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] How do different cohesins contribute to the connection between homologs in mammalian meiosis?2018

    • Author(s)
      Lee J.
    • Organizer
      IPR international seminar on “Genome stability and instability in mitotic and meiotic cells”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 繁殖生物学会 改訂版 第2章-1 『生殖細胞』2020

    • Author(s)
      李 智博,宮野 隆
    • Total Pages
      351
    • Publisher
      インターズー
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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