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Elucidation of the molecular mechanism to maintain hierarchical structure of vascular system and and tumour angiogenesis

Research Project

Project/Area Number 18H02363
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42040:Laboratory animal science-related
Research InstitutionShiga University of Medical Science

Principal Investigator

Ema Masatsugu  滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)

Co-Investigator(Kenkyū-buntansha) 水野 聖哉  筑波大学, 医学医療系, 准教授 (10633141)
杉山 文博  筑波大学, 医学医療系, 教授 (90226481)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords血管新生 / 血管内皮細胞 / マウス / 遺伝子改変 / リンパ管新生 / エキソサイトーシス / 腫瘍血管新生 / イメージング / ノックアウトマウス
Outline of Final Research Achievements

We have identified Exoc3-like family genes which are specifically expressed in developing endothelial cells. To investigate the physiological roles of Exoc3L genes, we have created Exoc3L2-GFP knock-in mouse and found that GFP (Exoc3L2) is expressed in endothelial cells and KO embryos die in utero due to breeding. We also created Exoc3L4-GFP knock-in mouse and found GFP (Exoc3L4) is expressed in endothelial cells as well as cardiomyocytes. Exoc3L4 KO embryos die around E12.5, due to unknown mechanism.
We also created VEGFR3-Venus BAC Tg mouse useful for visualization of vascular and lymphatic endothelial cells.

Academic Significance and Societal Importance of the Research Achievements

Exoc3L遺伝子群の生理機能を体系的に明らかにし、その制御機構を解明することができれば、新たな腫瘍血管新生抑制療法の開発に繋がるものと期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (1 results)

All 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells2019

    • Author(s)
      Watanabe C., Matsushita J., Azami T., Tsukiyama-Fujii S., Tsukiyama T., Mizuno S., Takahashi S., and Ema M.
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 1 Pages: e0210060-e0210060

    • DOI

      10.1371/journal.pone.0210060

    • NAID

      120007128325

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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