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Understanding of spatial regulation of sister chromatid cohesion

Research Project

Project/Area Number 18H02372
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionNagoya University

Principal Investigator

Nishiyama Tomoko  名古屋大学, 理学研究科, 准教授 (90615535)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywordsクロマチン / 姉妹染色分体間接着 / ヘテロクロマチン
Outline of Final Research Achievements

In this study, we aimed to understand the mechanisms of how sister chromatid cohesion in heterochromatin region is established. Dalmatian (Dmt) is known as a cohesion establishment factor in Drosophila melanogaster. Through non-biased screening of Dmt-binding proteins, we identified 25 candidate genes, of which knockdown exhibited abnormal cohesion. Among them, we found that BAP180, a subunit of PBAP chromatin remodeling complex, is essential role in cohesion. In BAP180-depleted cells, Dmt couldn’t be localized to heterochromatin, however cohesin itself normally associated with chromatin. Overexpression of Dmt restored cohesion in BAP180-depleted cells. These results suggest that BAP180 has a crucial role in cohesion establishment in heterochromatin by regulating Dmt localization.

Academic Significance and Societal Importance of the Research Achievements

これまでの接着研究においては、DNAとコヒーシンが一対一の関係で論じられ、DNA側を取り巻く環境との関連は考慮されて来なかった。本研究ではクロマチン環境と接着機能の関係に着目した初めての研究となる点で、学術的意義がある。さらに本研究の成果は、ヘテロクロマチン領域における複製や転写といった接着以外の現象の分子機構理解にも繋がるだけでなく、接着機構のヘテロクロマチン以外の染色体環境への適応機構を理解する手がかりにもなり、染色体研究分野に大きな波及効果をもたらすものと期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(9 results)

All 2021 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results)

  • [Journal Article] Opening of cohesin’s SMC ring is essential for timely DNA replication and DNA loop formation2021

    • Author(s)
      Sakata Ryota、Niwa Kyoma、Ugarte La Torre Diego、Gu Chenyang、Tahara Eri、Takada Shoji、Nishiyama Tomoko
    • Journal Title

      Cell Reports

      Volume: 35 Pages: 108999-108999

    • DOI

      10.1016/j.celrep.2021.108999

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Compartments in the Ring2019

    • Author(s)
      Nishiyama Tomoko
    • Journal Title

      Molecular Cell

      Volume: 75 Pages: 201-203

    • DOI

      10.1016/j.molcel.2019.07.002

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Cohesion and cohesin-dependent chromatin organization2019

    • Author(s)
      Nishiyama Tomoko
    • Journal Title

      Current Opinion in Cell Biology

      Volume: 印刷中

    • DOI

      10.1016/j.ceb.2018.11.006

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Opening of cohesin’s Smc ring is essential for timely DNA replication2021

    • Author(s)
      Tomoko Nihsiyama
    • Organizer
      第38回 染色体ワークショップ
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] コヒーシンの構造とゲノム機能2019

    • Author(s)
      西山 朋子
    • Organizer
      国立遺伝学研究所研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Impacts of cohesin ring conformation on the translocation and DNA replication2019

    • Author(s)
      Tomoko Nihsiyama
    • Organizer
      EMBO workshop Smc proteins
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics of cohesin at single-molecule resolution reveals cohesin-dependent chromatin structure2019

    • Author(s)
      Tomoko Nihsiyama
    • Organizer
      日本分子生物学会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Understanding cohesin dependent structural organization of DNA in single molecule resolution2019

    • Author(s)
      Tomoko Nihsiyama
    • Organizer
      Chromosome Dynamics 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Impacts of cohesin ring conformation on the translocation and DNA replication2018

    • Author(s)
      Tomoko Nishiyama
    • Organizer
      3C & 3R
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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