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The analysis of a novel machinery coordinating cell cycle progression with dynamic chromosome architecture

Research Project

Project/Area Number 18H02377
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionKyushu University

Principal Investigator

Ozaki Shogo  九州大学, 薬学研究院, 准教授 (70510147)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords染色体ダイナミクス / 細菌 / 細胞分裂 / 細胞周期 / DNA複製 / タンパク質複合体 / 細胞内局在 / バクテリア / 染色体 / 複製 / 分裂 / ゲノム複製 / 遺伝情報継承 / 時空間制御 / ダイナミクス / 細胞内シグナリング / 細菌ゲノム / シグナル伝達
Outline of Final Research Achievements

A fundamental of life is to coordinate the chromosome architecture with cell cycle progression in order to faithfully propagate genetic information. In this study, we aimed at addressing the molecular and cellular mechanisms controlling cell division in coordination with chromosome replication termination. We identified ZapT as a novel cell division protein associated with DNA. ZapT is required for normal cell division, ensuring strict colocalization of divisome with chromosome terminus. We show that ZapT has the affinity for both divisome and chromosome terminus, which help to organize the terminus DNA into a clustered structure over the divisome. Biochemical characterization revealed that ZapT and the divisome subunits ZauP and ZapA interacted directly to form a highly ordered ternary complex. Together, we propose that ZauP-dependent clustering of ZapT-DNA complexes plays a distinct role in organizing the replication terminus and the Z-ring.

Academic Significance and Societal Importance of the Research Achievements

細菌の増殖機構は我々の健康維持と直結する。細菌に起因する種々の問題に取り組むための基礎は「細菌増殖について分子レベルで詳細に理解すること」にある。そこで我々は、細菌増殖の本質である遺伝情報継承システムの解明を目指して研究を行なった。
本研究ではカウロバクターをモデル生物とし、複製終結点と細胞分裂装置とを連係する新規タンパク質ZapTを発見し、その分子細胞メカニズムを解明した。緑膿菌やブルセラ菌などの病原性細菌にもZapTホモログは保存されており、本研究成果はひろく細菌界で保存された原理の解明に貢献したと言える。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(38 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (29 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] University of Basel(スイス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] バーゼル大学(スイス)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Z-ring-associated proteins regulate clustering of the replication terminus-binding protein ZapT in Caulobacter Crescentus.2021

    • Author(s)
      Ozaki Shogo, Wakasugi Yasutaka, Tsutomu Katayama
    • Journal Title

      mBio

      Volume: 12 Pages: 1-17

    • DOI

      10.1128/mbio.02196-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DnaB helicase is recruited to the replication initiation complex via binding of DnaA domain I to the lateral surface of the DnaB N-terminal domain2020

    • Author(s)
      Hayashi Chihiro、Miyazaki Erika、Ozaki Shogo、Abe Yoshito、Katayama Tsutomu
    • Journal Title

      Journal of Biological Chemistry

      Volume: 295 Pages: 11131-11143

    • DOI

      10.1074/jbc.ra120.014235

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Divisome-Associated Protein Spatially Coupling the Z-Ring with the Chromosomal Replication Terminus in Caulobacter crescentus2020

    • Author(s)
      Ozaki Shogo、Jenal Urs、Katayama Tsutomu
    • Journal Title

      mBio

      Volume: 11 Pages: 1-19

    • DOI

      10.1128/mbio.00487-20

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Journal Article] Regulation of replication initiation: lessons from <i>Caulobacter crescentus</i>2019

    • Author(s)
      Ozaki Shogo
    • Journal Title

      Genes & Genetic Systems

      Volume: 94 Issue: 5 Pages: 183-196

    • DOI

      10.1266/ggs.19-00011

    • NAID

      130007760286

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel mode of DnaA-DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity2019

    • Author(s)
      Sugiyama, R., Kasho, K., Miyoshi, K., Ozaki, S., Kagawa, W., Kurumizaka, H., and Katayama, T
    • Journal Title

      Nucleic Acids Research

      Volume: 47 Pages: 11209-11224

    • DOI

      10.1093/nar/gkz795

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Escherichia coli CrfC Protein, a Nucleoid Partition Factor, Localizes to Nucleoid Poles via the Activities of Specific Nucleoid-Associated Proteins2019

    • Author(s)
      Saki Taniguchi, Kazutoshi Kasho, Shogo Ozaki, Tsutomu Katayama
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 1-18

    • DOI

      10.3389/fmicb.2019.00072

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The DnaA AAA+ Domain His136 Residue Directs DnaB Replicative Helicase to the Unwound Region of the Replication Origin, oriC2018

    • Author(s)
      Yukari Sakiyama, Masahiro Nishimura, Chihiro Hayashi, Yusuke Akama, Shogo Ozaki, Tsutomu Katayama
    • Journal Title

      Frontiers in Microbiology

      Volume: 9 Pages: 1-17

    • DOI

      10.3389/fmicb.2018.02017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 大腸菌における複製開始を制御するdatA領域の活性制御機構についての解析2021

    • Author(s)
      伊藤孝輔、酒井隆至、加生和寿、尾崎省吾、片山 勉
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高度好熱性真正細菌Thermotoga maritimaの複製開始複合体におけるDNA開裂領域の配列特性の解析2021

    • Author(s)
      盧 楚元、尾崎省吾、片山 勉
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大腸菌染色体の複製開始促進因子DARS2における活性化蛋白質の細胞周期依存的な結合制御機構2021

    • Author(s)
      三善賢弥、辰本優香、尾崎省吾、川上広宣、片山 勉
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細菌種に高度に保存されたHUによる染色体複製起点oriCの二重鎖開裂促進機構の解析2021

    • Author(s)
      吉田竜星、尾崎省吾、川上広宣、片山 勉
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 真正細菌Caulobacter crescentusにおいてZ-ringと染色体ter領域の共局在を制御する高次複合体のメカニズム2021

    • Author(s)
      若杉泰敬、尾崎省吾、片山 勉
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular mechanisms of specific nucleoid proteins in regulations for the replication initiation of the Escherichia coli chromosome2020

    • Author(s)
      三善 賢弥、吉田 竜星、盧 楚元、辰本 優香、李 藍楊、興梠 和真、伊藤 孝輔、加生 和寿、川上 広宣、永田 麻梨子、尾崎 省吾
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] カウロバクター細胞内におけるDnaBヘリカーゼの複製起点への集合、装着動態の解析2020

    • Author(s)
      村岡 龍哉、尾崎省吾、片山 勉
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大腸菌染色体の複製起点における一本鎖DNA形成の動的メカニズムとDnaBヘリカーゼ 装着における意義2020

    • Author(s)
      永田麻梨子、崎山友香里、尾﨑省吾、片山 勉
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 染色体複製終点と細胞分裂装置とを連係する新規DNA結合タンパク質ZapTの解析2020

    • Author(s)
      尾崎省吾、若杉泰敬、片山勉
    • Organizer
      日本遺伝学会第92回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 真正細菌カウロバクターの複製終結点結合蛋白質ZapTは細胞分裂装置を介して高次複合体を形成する2020

    • Author(s)
      若杉泰敬、尾崎省吾、片山 勉
    • Organizer
      日本遺伝学会第92回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大腸菌における染色体の複製開始促進因子DARS2を活性化する核様体蛋白質の適時的な結合制御機構の解析2020

    • Author(s)
      三善賢弥、辰本優香、尾崎省吾、永田麻梨子、片山 勉
    • Organizer
      日本遺伝学会第92回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 配列非特異的なDNA結合因子HUによる複製起点oriCの二重鎖開裂機構の生化学・遺伝学的解析2020

    • Author(s)
      吉田竜星、尾崎省吾、川上広宣、片山 勉
    • Organizer
      日本遺伝学会第92回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 超好熱性真正細菌Thermotoga maritima の複製開始複合体中でDnaAと特異的に結合する1本鎖DNAの配列特性の解析2020

    • Author(s)
      盧 楚元、尾崎省吾、片山 勉
    • Organizer
      日本遺伝学会第92回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大腸菌染色体の複製開始を促進する非コード領域DARS2でのATP結合型DnaA複合体形成と機能解析2020

    • Author(s)
      辰本優香、三善賢弥、永田麻梨子、尾崎省吾、片山 勉
    • Organizer
      2020年度日本生化学会九州支部例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] モデル細菌Caulobacter cresentusにおける複製終結点terと細胞分裂装置の共局在を調節する 蛋白質間相互作用の解析2020

    • Author(s)
      若杉泰敬、尾崎省吾、片山 勉
    • Organizer
      2020年度日本生化学会九州支部例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高度好熱性真正細菌Thermotoga maritimaの複製起点oriCにおいてDnaAと特異的相互作用する一本鎖 DNAモチーフの探索2020

    • Author(s)
      盧 楚元、尾崎 省吾、片山 勉
    • Organizer
      2020年度日本生化学会九州支部例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] A novel divisome-associated protein spatially couples the Z-ring with the chromosomal replication terminus in Caulobacter crescentus2020

    • Author(s)
      Shogo Ozaki、Yasutaka Wakasugi、Urs Jenal、 Tsutomu Katayama
    • Organizer
      CAULOCONFERENCE 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 染色体複製終点と細胞分裂装置とを連係する新規DNA結合タンパク質の解析2020

    • Author(s)
      尾崎省吾、若杉泰敬、片山勉
    • Organizer
      第14回日本ゲノム微生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カウロバクター菌における染色体複製と細胞分裂とを連係する機構の解析2020

    • Author(s)
      尾崎省吾、若杉泰敬、片山勉
    • Organizer
      第93回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification and characterization of a novel DNA binding protein that colocalizes the chromosome replication terminus with cell division apparatus in Caulobacter crescentus2019

    • Author(s)
      尾崎省吾、若杉泰敬、片山勉
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis for mechanisms of DnaB helicase loading by specific DnaA subcomplexes for bidirectional replication of the E. coli chromosome2019

    • Author(s)
      永田 麻梨子, 崎山 友香里, 林千尋, 尾崎 省吾, 片山 勉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dynamic mechanisms of higher-order complexes for replication initiation and regulation of the E. coli genome2019

    • Author(s)
      片山勉, 三善賢弥, 林千尋, 吉田竜星, 杉山諒, 酒井隆至, 崎山友香里, 加生和寿, 川上広宣, 尾崎省吾, 永田麻梨子
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 非コードDNA因子DARSにおける複製開始蛋白質DnaAの複合体形成と活性化メカニズム2019

    • Author(s)
      三善賢弥、杉山諒、加生和寿、尾崎省吾、片山勉
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大腸菌染色体の非コード領域DARSにおけるDnaA AAA+ドメインの新たな複合体形成と動態2019

    • Author(s)
      三善賢弥、杉山諒、加生和寿、尾崎省吾、片山勉
    • Organizer
      2019年度 生化学会九州支部例会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 有柄細菌カウロバクターにおける染色体複製終結点結合タンパク質の特性解析2019

    • Author(s)
      若杉泰敬、尾崎省吾、片山勉
    • Organizer
      2019年度 生化学会九州支部例会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 核様体タンパク質 HU による大腸菌 oriC の二重鎖開裂促進機構の生化学的解析2019

    • Author(s)
      吉田竜星、尾崎省吾、川上広宣、片山勉
    • Organizer
      第16回大腸菌研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The role of the bacterial second messenger signaling for chromosome segregation dynamics in Caulobacter crescentus2018

    • Author(s)
      Shogo Ozaki, Urs Jenal, Yasutaka Wakasugi, Tsutomu Katayama
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The role of the second messenger signalling for coordinating chromosome replication in time and space.2018

    • Author(s)
      Shogo Ozaki, Christian Lori, Badri N Dubey, Urs Jenal
    • Organizer
      日本遺伝学会第90回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The second messenger signaling drives chromosome replication in the asymmetrically dividing bacterium Caulobacter crescentus2018

    • Author(s)
      Shogo Ozaki, Lori Christian, Urs Jenal
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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