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Structural analysis of ion-bound state and transport mechanism of microbial rhodopsin by multinuclear solid-state NMR of microbial rhodopsins

Research Project

Project/Area Number 18H02387
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43020:Structural biochemistry-related
Research InstitutionYokohama National University

Principal Investigator

Kawamura Izuru  横浜国立大学, 大学院工学研究院, 准教授 (20452047)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords固体NMR / レチナール / 化学シフト / イオン / 多核NMR / 膜タンパク質 / ロドプシン / イオン輸送
Outline of Final Research Achievements

Membrane proteins play an important role in maintaining cell homeostasis. Among them, microbial rhodopsin is covalently bound to retinal chromophore via a protonated Schiff base, and the protein is activated by photo-isomerization. Solid-state NMR spectroscopy with multinuclear observations as well as 13C and 15N was applied to detect important interactions containing ion-bound states, contacts with water molecules, and aromatic stacking. It is found that monitoring of the alterations in NMR signals can find local structural change of microbial rhodopsin depending on pH, replacement of alkali metal ions, H/D exchange and point mutation. Furthermore, we developed a methodology such as In-situ microwave irradiation NMR method to investigate the non-thermal effects on polar group in a molecule.

Academic Significance and Societal Importance of the Research Achievements

膜タンパク質の反応中心における特定の水素結合や静電的な相互作用などは膜タンパク質の重要な相互作用として特徴付けられ、分子レベルでそれらの機能を理解するためのアプローチとなる。そのような相互作用に対して、構造生物化学の分野で頻繁に利用される1H, 13C, 15N核以外にも多核的な観測によって、さらに深くアプローチできることを示した。今後、微生物型ロドプシンをはじめとした多くの膜タンパク質の動的構造の解明に活用できるものと期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(64 results)

All 2021 2020 2019 2018 Other

All Journal Article (30 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 26 results,  Open Access: 4 results) Presentation (31 results) (of which Int'l Joint Research: 12 results,  Invited: 9 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Structural determination of the sheath of Sphaerotilus montanus using thiopeptidoglycan lyase which cleaves the 1,4 linkage between α-D-GalN and β-D-GlcA2021

    • Author(s)
      1. D. Kasiwabara, K. Kondo, R. Usami, D. Kan, I. Kawamura, Y. Kawasaki, M. Sato, T. Nittami, I. Suzuki, M. Katahira, M. Takeda
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 183 Pages: 992-1001

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vibrational Circular Dichroism towards Asymmetric Catalysis: Chiral Induction in Substrates Coordinated with Copper(II) Ions2021

    • Author(s)
      H. Sato, K. Takimoto, J. Yoshida, A. Yamagishi
    • Journal Title

      Analytical Chemistry

      Volume: 22 Pages: 24393-24398

    • DOI

      10.1021/acs.analchem.0c02990

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermal and non-thermal microwave effects of ethanol and hexane mixed solution as revealed by in situ microwave irradiation nuclear magnetic resonance spectroscopy and MD simulation2020

    • Author(s)
      Yugo Tasei, Batsaikhan Mijiddorj, Teruaki Fujito, Izuru Kawamura, Kazuyoshi Ueda, Akira Naito
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 124 Pages: 9615-9624

    • DOI

      10.1021/acs.jpcb.0c06383

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Characterization of a Cyclodextrin/<i>l</i>-menthol Inclusion Complex in the Solid-state by Solid-state NMR and Vibrational Circular Dichroism2020

    • Author(s)
      Takahiro SAKAI, Yumiko AKAGI, Hisashi SUZUKI, Mitsuki IRIE, Tetsuya NAKAMURA, Hisako SATO, Izuru KAWAMURA*
    • Journal Title

      Anal. Sci.

      Volume: 36 Issue: 11 Pages: 1337-1343

    • DOI

      10.2116/analsci.20p120

      10.2116/analsci.20P120

    • NAID

      130007937740

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stereochemical Effects on the Self-Assembly of Pyrenylalanine-Phenylalanine Dipeptide2020

    • Author(s)
      Mijiddorj Batsaikhan, Shirakata Hiroki, Nakagawa Tetsuya, Ueda Kazuyoshi, Yokoyama Yasushi, Kawamura Izuru
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 93 Pages: 969-977

    • DOI

      10.1246/bcsj.20190376

    • NAID

      130007888002

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 食品物理学Food Biophysicsのススメ2020

    • Author(s)
      川村出
    • Journal Title

      ハセガワレター

      Volume: 39 Pages: 2-11

    • Related Report
      2020 Annual Research Report
  • [Journal Article] コーヒー粕を原料としたセルロースナノファイバーとコンポジットフィルム2020

    • Author(s)
      金井典子, 川村出
    • Journal Title

      コンバーテック

      Volume: 569 Pages: 116-119

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Structural characterization of cellulose nanofibers isolated from spent coffee grounds and their composite films with poly(vinyl alcohol): a new non-wood source2020

    • Author(s)
      Noriko Kanai, Takumi Honda, Naoki Yoshihara, Toshiyuki Oyama, Akira Naito, Kazuyoshi Ueda, Izuru Kawamura*
    • Journal Title

      Cellulose

      Volume: 27 Pages: 5017-5028

    • DOI

      10.1007/s10570-020-03113-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Separation of D-amino acid-containing peptide phenylseptin using 3,3′-phenyl-1,1′-binaphthyl-18-crown-6-ether columns2020

    • Author(s)
      Izuru Kawamura*, Batsaikhan Mijiddorj, Yohei Kayano, Yuta Matsuo, Yumi Ozawa, Kazuyoshi Ueda, Hisako Sato
    • Journal Title

      BBA - Proteins and Proteomics

      Volume: 1868 Pages: 140429-140429

    • DOI

      10.1016/j.bbapap.2020.140429

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 31P and 13C solid-state NMR analysis of morphological changes of phospholipid bilayers containing glucagon during fibril formation of glucagon under neutral condition2020

    • Author(s)
      Kazumi Haya, Yoshiteru Makino, Akie Kikuchi-Kinoshita, Izuru Kawamura, Akira Naito*
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1862 Pages: 183290-183290

    • DOI

      10.1016/j.bbamem.2020.183290

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rheological and Ionic Transport Properties of Nanocomposite Electrolytes Based on Protic Ionic Liquids and Silica Nanoparticles2020

    • Author(s)
      Marium Mayeesha、Hoque Mahfuzul、Miran Muhammed Shah、Thomas Morgan L.、Kawamura Izuru、Ueno Kazuhide、Dokko Kaoru、Watanabe Masayoshi
    • Journal Title

      Langmuir

      Volume: 36 Pages: 148-158

    • DOI

      10.1021/acs.langmuir.9b02848

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Soft chromophore featured liquid porphyrins and their utilization toward liquid electret applications2019

    • Author(s)
      Ghosh Avijit、Yoshida Manabu、Suemori Kouji、Isago Hiroaki、Kobayashi Nagao、Mizutani Yasuhisa、Kurashige Yuki、Kawamura Izuru、Nirei Masami、Yamamuro Osamu、Takaya Tomohisa、Iwata Koichi、Saeki Akinori、Nagura Kazuhiko、Ishihara Shinsuke、Nakanishi Takashi
    • Journal Title

      Nature Commun.

      Volume: 10 Pages: 4210-4210

    • DOI

      10.1038/s41467-019-12249-8

    • NAID

      120006898089

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interaction of Clear Flavor Emulsions Containing Lemon Essential Oils with Lipid Bilayers via a Quartz Crystal Microbalance2019

    • Author(s)
      Sakai Takahiro、Seki Hayato、Yoshida Shogo、Hori Hayato、Suzuki Hisashi、Nakamura Tetsuya、Kawamura Izuru
    • Journal Title

      Food Science and Technology Research

      Volume: 25 Pages: 879-884

    • DOI

      10.3136/fstr.25.879

    • NAID

      130007771310

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A comparative study of interactions of antimicrobial peptides L- and D-phenylseptin with 1,2-dimyristoyl-sn-glycero-3-phosphocholine2019

    • Author(s)
      Batsaikhan Mijiddorj, Yuta Matsuo, Hisako Sato, Kazuyoshi Ueda, Izuru Kawamura*
    • Journal Title

      Applied Sciences

      Volume: 9 Pages: 2601-2601

    • DOI

      10.3390/app9132601

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Application of Solid-State Vibrational Circular Dichroism for Intercalation Compounds of Layered Double Hydroxide and Amino Acids: Conformation of an Intercalated Phenylalanine2019

    • Author(s)
      Hisako Sato, Kazuyoshi Takimoto, Izuru Kawamura, Sumio Aisawa
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 92 Issue: 10 Pages: 1779-1782

    • DOI

      10.1246/bcsj.20190161

    • NAID

      130007728637

    • ISSN
      0009-2673, 1348-0634
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Solid-state vibrational circular dichroism studies of L- and D-serine2019

    • Author(s)
      Izuru Kawamura, Hisako Sato
    • Journal Title

      Analytical Biochemistry

      Volume: 580 Pages: 14-20

    • DOI

      10.1016/j.ab.2019.05.016

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of D-amino acid containing antimicrobial peptide derived from frog2019

    • Author(s)
      川村 出、Mijiddorj Batsaikhan、大山 暁史、佐藤 久子
    • Journal Title

      生化学

      Volume: 91 Issue: 3 Pages: 316-321

    • DOI

      10.14952/SEIKAGAKU.2019.910316

    • ISSN
      0037-1017
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Self-assembly of tripeptides into gamma-turn nanostructures2019

    • Author(s)
      Yumi Ozawa, Hisako Sato, Yohei Kayano, Nana Yamaki, Yu-ichiro Izato, Atsumi Miyake, Akira Naito, Izuru Kawamura
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: - Pages: 10879-10883

    • DOI

      10.1039/c9cp00233b

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrophysiological analysis of membrane disruption by Bombinin and its isomer using lipid bilayer system2019

    • Author(s)
      Yusuke Sekiya, Keisuke Shimizu, Yuki Kitahashi, Akifumi Oyama, Izuru Kawamura, Ryuji Kawano
    • Journal Title

      ACS Applied Bio Materials

      Volume: 2 Pages: 1542-1548

    • DOI

      10.1021/acsabm.8b00835

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoreaction pathways and photointermediates of retinal-binding photoreceptor proteins as revealed by in situ photoirradiation solid-state NMR spectroscopy2019

    • Author(s)
      Akira Naito, Yoshiteru Makino, Arisu Shigeta, Izuru Kawamura
    • Journal Title

      Biophysical Reviews

      Volume: 11 Pages: 167-181

    • DOI

      10.1007/s12551-019-00501-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory regulation mechanism of naphthoquinone and its derivatives in radical polymerization2019

    • Author(s)
      Kenta Ichihara, Izuru Kawamura, Kazuhisa Sakakibara, Yujirou Ikejiri, Shunichi Himori
    • Journal Title

      Journal of Organic Physical Chemistry

      Volume: -

    • DOI

      10.1002/poc.3941

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ロドプシンの光中間体の構造を解明 -タンパク質のダイナミックな構造変化を捉える光照射-固体NMR技術2019

    • Author(s)
      川村出
    • Journal Title

      化学同人 月刊 「化学」

      Volume: 3 Pages: 38-41

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Fibrillation mechanism of glucagon in the presence of phospholipid bilayers as revealed by 13C solid state NMR spectroscopy2019

    • Author(s)
      Izumi Yamane, Ayano Momose, Hideki Fujita, Eri Yoshimoto, Akie Kikuchi-Kinoshita, Izuru Kawamura, Akira Naito
    • Journal Title

      Chem. Phys. Lipids

      Volume: 219 Pages: 36-44

    • DOI

      10.1016/j.chemphyslip.2019.01.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Solid-state NMR characterization of triacylglycerol and polysaccharides in coffee beans2019

    • Author(s)
      Noriko Kanai, Naoki Yoshihara, Izuru Kawamura
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 83 Pages: 803-809

    • DOI

      10.1080/09168451.2019.1571899

    • NAID

      120006633343

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tricolor mechanochromic luminescence of an organic two-component dye: visualization of a crystalline state and two amorphous states2019

    • Author(s)
      Suguru Ito, Genki Katada, Tomohiro Taguchi, Izuru Kawamura, Takashi Ubukata, Masatoshi Asami
    • Journal Title

      CrystEngComm

      Volume: 21 Pages: 53-59

    • DOI

      10.1039/c8ce01698d

    • NAID

      120006550283

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Solid-state NMR characterization of the structure of self-assembled Ile-Phe-OH2019

    • Author(s)
      Izuru Kawamura, Hiroki Shirakata and Yumi Ozawa, Batsaikhan Mijiddorj, Kazuyoshi Ueda
    • Journal Title

      Magnetochemistry

      Volume: 4 Pages: 30-30

    • DOI

      10.3390/magnetochemistry4030030

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 動的核分極による超高感度固体NMRの現状2018

    • Author(s)
      川村 出
    • Journal Title

      ぶんせき (日本分析化学会)

      Volume: 11 Pages: 497-498

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retinal configuration of ppR intermediates revealed by photo-irradiation solid-state NMR and DFT2018

    • Author(s)
      Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Naoki Kamo, Yuki Sudo, Kazuyoshi Ueda, Akira Naito
    • Journal Title

      Biophysical Journal

      Volume: 115 Pages: 72-83

    • DOI

      10.1016/j.bpj.2018.05.030

    • NAID

      120006698118

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High thermal stability of oligomeric assemblies of Thermophilic rhodopsin in a lipid environment.2018

    • Author(s)
      Tomomi Shionoya, Misao Mizuno, Takashi Tsukamoto, Kento Ikeda, Hayato Seki, Keiichi Kojima, Mikihiro Shibata, Izuru Kawamura, Yuki Sudo, and Yasuhisa Mizutani
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 122 Pages: 6945-6953

    • DOI

      10.1021/acs.jpcb.8b04894

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生体膜内でのペプチドの挙動の分子動力学シミュレーション2018

    • Author(s)
      上田一義、内藤 晶、川村 出、Namsrai Javkhlantugs
    • Journal Title

      酵素工学ニュース(酵素工学会)

      Volume: 79 Pages: 27-30

    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Presentation] Solid-state NMR of seven-transmembrane proteins2020

    • Author(s)
      Izuru Kawamura
    • Organizer
      9th (biennial) Western Sydney University & Inaugural Asian Symposium on NMR, MRI & Diffusion symposium
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Solid-state vibrational circular dichroism of tripeptides2020

    • Author(s)
      Izuru Kawamura & Hisako Sato
    • Organizer
      Molecular Chirality 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Interaction of amphibian-derived cationic antimicrobial peptides with model membrane2020

    • Author(s)
      川村 出
    • Organizer
      The 58th Annual Meeting of Biophysical Society in Japan
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Solid-state NMR studies of seven-transmembrane ion-pumping rhodopsin and self-assembled peptide2019

    • Author(s)
      Izuru Kawamura
    • Organizer
      BCEIA2019 (Beijing, China)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 固体NMRを用いた膜タンパク質ロドプシンの細胞膜中における構造解析2019

    • Author(s)
      川村 出
    • Organizer
      ISSP WS レチナールタンパク質国際会議ワークショップ, 東京大学 物性研
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 固体NMRによる微生物型ロドプシンの立体構造解析2019

    • Author(s)
      川村 出
    • Organizer
      北海道大学 大学院生命科学研究院
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Solid-state NMR study of seven-transmembrane microbial rhodopsins2019

    • Author(s)
      Izuru Kawamura
    • Organizer
      The 1st Asian-European International Exchange Symposium in Solid State NMR, RIKEN Yokohama
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 脂質膜中にナノポアを構築するβシートペプチドのDe novo配列設計2019

    • Author(s)
      清水 啓佑, 坂下 峻吾, 浜田 芳男, 臼井 健二, Mijiddorj Batsaikhan, 川村 出, 川野 竜司
    • Organizer
      第57回日本生物物理学会年会, 宮崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] カチオン性抗菌ペプチドHymenochirin-1Paおよび変異体D9Kの細菌膜結合構造と膜選択性の解析2019

    • Author(s)
      大山 暁史, Batsaikhan Mijiddorj, 上田 一義, 内藤 晶, 川村 出
    • Organizer
      第57回日本生物物理学会年会, 宮崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] 固体NMRによるKR2のレチナール結合ポケットとNa+結合サイト間の水素結合ネットワークの構造解析2019

    • Author(s)
      金子 莉奈, 重田 安里寿, 長島 敏雄, 山崎 俊夫, 井上 圭一, 神取 秀樹, 川村 出
    • Organizer
      第57回日本生物物理学会年会, 宮崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] 多次元固体NMRによる細胞膜中のヘリオロドプシンの構造解析2019

    • Author(s)
      鈴木 しぶき, 長島 敏雄, 金子 莉奈, 沖津 貴志, 和田 昭盛, 小林 直宏, 山崎 俊夫, 吉住 玲, 井上 圭一, 神取 秀樹, 川村 出
    • Organizer
      第57回日本生物物理学会年会, 宮崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] A comparative study of membrane-bound structure of antimicrobial peptides L- and D-phenylseptin2019

    • Author(s)
      Izuru Kawamura, Batsaikhan Mijiddorj, Hisako Sato, Yuta Matsuo, Akira Naito, Kazuyoshi Ueda
    • Organizer
      第57回日本生物物理学会年会, 宮崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] 多次元固体NMRによるヘリオロドプシンの構造解析2019

    • Author(s)
      鈴木 しぶき, 長島 敏雄, 金子 莉奈, 沖津 貴志, 和田 昭盛, 小林 直宏, 山崎 俊夫, 吉住 玲, 井上 圭一, 神取 秀樹, 川村 出
    • Organizer
      第58回NMR討論会, 川崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] 多次元固体MAS NMRによる光駆動型Na+ポンプロドプシンKR2の構造解析2019

    • Author(s)
      金子 莉奈, 重田 安里寿, 長島 敏雄, 山崎 俊夫, 井上 圭一, 神取 秀樹, 川村 出
    • Organizer
      第58回NMR討論会, 川崎
    • Related Report
      2019 Annual Research Report
  • [Presentation] 固体NMRを用いた粉末試料の構造解析2018

    • Author(s)
      川村出
    • Organizer
      日本セラミックス協会 第31回秋季シンポジウム 「先進セラミックス開発のための粉体プロセス最前線」, 名古屋工業大学
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 両生類由来の抗菌ペプチドに含まれるDアミノ酸残基の役割の解明2018

    • Author(s)
      川村出
    • Organizer
      第14回Dアミノ酸学会学術講演会, 富山大学
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Multi-dimensional solid-state NMR of KR2 structure2018

    • Author(s)
      ◯Rina Kaneko, Arisu Shigeta, Toshio Nagashima, Toshio Yamazaki, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
    • Organizer
      The 18th International Conference on Retinal Proteins (ICRP2018), Toronto, Sep. 23-29, 2018 (Poster)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural changes in retinal-binding site of sodium ion pumping rhodopsin KR2 induced by His30 and Na+ binding site at the extracellular side2018

    • Author(s)
      ◯Izuru Kawamura, Arisu Shigeta, Shota Ito, Rina Kaneko, Sahoko Tomida, Keiichi Inoue, Hideki Kandori
    • Organizer
      The 18th International Conference on Retinal Proteins (ICRP2018), Toronto, Sep. 23-29, 2018 (Oral)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Stereochemistry-dependent nanostructure of phenylalanine-based tripeptide self-assembly2018

    • Author(s)
      ◯Yumi Ozawa, Hisako Sato, Nana Yamaki, Yuichiro Izao, Atsumi Miyake, Akira Naito, Izuru Kawamura
    • Organizer
      Asian Pacific Prion Symposium 2018 (APPS2018), Tokyo Metropolitan Industrial Technology Research Institute, Japan, Oct. 4-5, 2018. (Poster)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Controlling the self-assembly of tri-peptide with d-amino acid and probing the nano-structural difference2018

    • Author(s)
      ◯Yumi Ozawa, Hisako Sato, Akira Naito, Izuru Kawamura
    • Organizer
      Applied Nanotechnology and Nanoscience International Conference (ANNIC 2018), Berlin, Oct. 22-24, 2018. (Oral)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural characterization of Cellulose Nanofibers Prepared from Spent Coffee Grounds2018

    • Author(s)
      ◯Noriko Kanai, Naoki Yoshihara, Kazuyoshi Ueda, Izuru Kawamura
    • Organizer
      Applied Nanotechnology and Nanoscience International Conference (ANNIC 2018), Berlin, Oct. 22-24, 2018. (Oral)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Controlling the self-assembly of tri-peptide with d-amino acid and probing the nano-structural difference2018

    • Author(s)
      ◯Yumi Ozawa, Hisako Sato, Nana Yamaki, Yuichiro Izao, Atsumi Miyake, Akira Naito, Izuru Kawamura
    • Organizer
      ISEAN 2018, Australia, Oct. 29-Nov. 2, 2018. (Poster)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Membrane-bound structure and membrane selectivity of cationic antimicrobial peptide Hymenochirin-1Pa as studied by circular dichroism, solid-state NMR and molecular dynamics simulation2018

    • Author(s)
      ◯Akifumi Oyama, Batsaikhan Mijiddorj, Kazuyoshi Ueda, Izuru Kawamura
    • Organizer
      The 10th International Peptide Symposium, ROHM theater, Kyoto, Dec. 3-7, 2018. (Poster) JPS Excellent Poster Presentation Award受賞
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Membrane interactions of a D-amino acid containing antimicrobial peptides as revealed by experimental and theoretical methods2018

    • Author(s)
      ◯Batsaikhan Mijiddorj, Shiho Kaneda, Hisako Sato, Yuta Matsuo, Namsrai Javkhlantugs, Akira Naito, Kazuyoshi Ueda Izuru Kawamura
    • Organizer
      The 10th International Peptide Symposium, ROHM theater, Kyoto, Dec. 3-7, 2018. (Poster)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多核固体NMRによるコーヒー豆細胞壁の構造解析2018

    • Author(s)
      ◯金井典子, 吉原直希, 川村 出
    • Organizer
      18-1 NMR研究会, 理化学研究所・横浜キャンパス 交流棟1F, 2018年5月9日 (Poster)
    • Related Report
      2018 Annual Research Report
  • [Presentation] コーヒー豆由来セルロースの固体NMR構造解析2018

    • Author(s)
      ◯金井典子, 吉原直希, 上田一義, 川村 出
    • Organizer
      セルロース学会 第25回年次大会, 京都大学 宇治キャンパス, 2018年7月5-6日 (Poster) 優秀ポスター賞受賞
    • Related Report
      2018 Annual Research Report
  • [Presentation] ピレン側鎖を導入したジペプチドの自己組織化と蛍光特性2018

    • Author(s)
      ◯白方宏樹, 中川哲也, 横山 泰, 川村 出
    • Organizer
      光化学討論会, 関西学院大学, 2018年9月5-7日
    • Related Report
      2018 Annual Research Report
  • [Presentation] The effects of different alkali metal ions on KR2 structure revealed by multi-dimensional solid-state NMR2018

    • Author(s)
      ◯Rina Kaneko, Arisu Shigeta, Toshio Nagashima, Toshio Yamazaki, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
    • Organizer
      第56回日本生物物理学会, 岡山大学, 2018年9月15-17日
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural changes in retinal-binding site of the Krokinobacter rhodopsin 2 mutant H30A2018

    • Author(s)
      ◯Izuru Kawamura, Arisu Shigeta, Shota Ito, Rina Kaneko, Sahoko Tomida Keiichi Inoue, Hideki Kandori
    • Organizer
      第56回日本生物物理学会, 岡山大学, 2018年9月15-17日
    • Related Report
      2018 Annual Research Report
  • [Presentation] 固体MAS NMRによる光駆動型ナトリウムイオンポンプロドプシンKR2のアルカリ金属イオン依存的な構造変化の解析2018

    • Author(s)
      ◯金子莉奈, 重田安里寿, 長島敏雄, 山崎俊夫, 井上圭一, 神取秀樹, 川村出
    • Organizer
      第57回NMR討論会, 札幌コンベンションセンター, 2018年9月18-20日
    • Related Report
      2018 Annual Research Report
  • [Presentation] 固体NMRによる自己組織化ペプチドの構造解析2018

    • Author(s)
      ◯川村 出, 白方宏樹, 尾澤夢実, 佐藤久子、Batsaikhan Mijiddorj, 上田一義, 内藤晶
    • Organizer
      第57回NMR討論会, 札幌コンベンションセンター, 2018年9月18-20日
    • Related Report
      2018 Annual Research Report
  • [Book] 膜タンパク質工学ハンドブック 第1編第2章第10節を担当2020

    • Author(s)
      川村 出
    • Total Pages
      537
    • Publisher
      NTS出版
    • ISBN
      9784860435370
    • Related Report
      2019 Annual Research Report
  • [Book] NMR Methods for Characterization of Synthetic and Natural Polymers / Chap. 19の執筆を担当2019

    • Author(s)
      Arisu Shigeta, Izuru Kawamura*
    • Total Pages
      565
    • Publisher
      Royal Society of Chemistry
    • ISBN
      9781788014007
    • Related Report
      2019 Annual Research Report
  • [Remarks] 横浜国立大学 研究者総覧

    • URL

      https://er-web.ynu.ac.jp/html/KAWAMURA_Izuru/ja.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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