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Structural study on the active binding of dynein/dynactin complex to the microtubule

Research Project

Project/Area Number 18H02390
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43020:Structural biochemistry-related
Research InstitutionOsaka University

Principal Investigator

Kurisu Genji  大阪大学, 蛋白質研究所, 教授 (90294131)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords構造生物学 / 分子モーター / ダイニン / 微小管 / 構造生物化学 / ダイナクチン
Outline of Final Research Achievements

We attempted to crystallize a complex of dynein stalk-MTBD region with LC1 and the αβ-tubulin dimer, but were unable to determine the structure of the complex, although microcrystals were obtained. Furthermore, the CAP-Gly region of dynactin p150 was also analyzed, after evaluating its interaction with αβ-tubulin dimer, and then the structure of the complex was analyzed by cryo-EM. However again, the structure could not be determined at a high resolution. In the course of these structural analyses, we obtained the crystals of the much better MTBD fusion protein alone, which has high microtubule-binding capacity. In addition, from the structural analysis of the complex of the MTBD region of the outer-arm dynein γ-chain and the light chain LC1, we were able to propose a novel binding mode of MTBD to the microtubule utilizing the flap region.

Academic Significance and Societal Importance of the Research Achievements

微小管を移動する分子モーターは,プラス端方向に移動するキネシンとマイナス端方向に移動するダイニンの2種類がしられている。キネシンの運動メカニズムについては,複数の視点で非常に詳細な情報が蓄積している。これに対しダイニンは,鞭毛・繊毛運動を駆動し,染色体分離や細胞内輸送を担う非常に重要な生体分子であるにも関わらず,モータードメインの分子サイズが380 kDaと巨大であるため,最近まで原子レベルの構造解明が遅れていた。本研究は理解が遅れていたダイニンの運動機構解明に貢献するものであり,今後の展開も期待できる。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (13 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Connecticut(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Connecticut(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Connecticut(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] The Complex of Outer-arm Dynein Light Chain-1 and the Microtubule-binding Domain of the Heavy Chain Shows How Axonemal Dynein Tunes Ciliary Beating2021

    • Author(s)
      Toshiki Yagi, Akiyuki Toda, Muneyoshi Ichikawa, Genji Kurisu
    • Journal Title

      Seibutsu Butsuri

      Volume: 61 Issue: 1 Pages: 020-022

    • DOI

      10.2142/biophys.61.020

    • NAID

      130007976664

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The complex of outer-arm dynein light chain-1 and the microtubule-binding domain of the γ heavy chain shows how axonemal dynein tunes ciliary beating2020

    • Author(s)
      Toda Akiyuki、Nishikawa Yosuke、Tanaka Hideaki、Yagi Toshiki、Kurisu Genji
    • Journal Title

      Journal of Biological Chemistry

      Volume: 295 Issue: 12 Pages: 3982-3989

    • DOI

      10.1074/jbc.ra119.011541

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Continuum mechanical parameterisation of cytoplasmic dynein from atomistic simulation2020

    • Author(s)
      Hanson Benjamin S.、Iida Shinji、Read Daniel J.、Harlen Oliver G.、Kurisu Genji、Nakamura Haruki、Harris Sarah A.
    • Journal Title

      Methods

      Volume: - Pages: 39-48

    • DOI

      10.1016/j.ymeth.2020.01.021

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Copper‐oxygen Dynamics in Tyrosinase Mechanism2020

    • Author(s)
      Fujieda Nobutaka、Umakoshi Kyohei、Ochi Yuta、Nishikawa Yosuke、Yanagisawa Sachiko、Kubo Minoru、Kurisu Genji、Itoh Shinobu
    • Journal Title

      Angewandte Chemie International Edition

      Volume: - Issue: 32 Pages: 13385-13390

    • DOI

      10.1002/anie.202004733

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural atlas of dynein motors at atomic resolution2018

    • Author(s)
      Akiyuki Toda, Hideaki Tanaka, Genji Kurisu
    • Journal Title

      Biophys Rev.

      Volume: 10 Issue: 2 Pages: 677-686

    • DOI

      10.1007/s12551-018-0402-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Structural Analysis of Dynactin p150Glued and microtubule complex2022

    • Author(s)
      Yu Michimoto, Shiho Yanagi, Akiyuki Toda, Hideaki Tanaka, Akihiro Kawamoto, Genji Kurisu
    • Organizer
      日本化学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ダイナクチンp150GluedがもつCoiled-Coil 1領域のX線結晶構造解析2019

    • Author(s)
      柳志歩, 戸田暁之, 小林琢也,斎藤慧, 豊島陽子, 栗栖源嗣
    • Organizer
      生体運動班会議2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural insights into complex formation of the axonemal dynein light chian-1 and OADgamma stalk2018

    • Author(s)
      A. Toda, H. Tanaka, Y. Nishikawa, T. Yagi & G. Kurisu
    • Organizer
      Gordon Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 蛋白質結晶学研究室

    • URL

      http://www.protein.osaka-u.ac.jp/crystallography/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 蛋白質結晶学研究室ホームページ

    • URL

      http://www.protein.osaka-u.ac.jp/crystallography/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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