Development of Protein Design Methods and Their Experimental Verification
| Project/Area Number |
18H02395
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 43020:Structural biochemistry-related
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Zhang Kam 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (60558906)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
|---|
| Keywords | 計算タンパク質設計 / Protein design / Computation / Evolutionary algorithm / Protein folding / Repeat proteins / Fragment assembly / Protein Design / EDA method |
|---|
| Outline of Final Research Achievements |
We have developed a computational protein design method with backbone flexibility, which exploits local structural environments in known protein structures together with energy to guide sequence design. It is based on customized libraries of non-contiguous in-contact amino acid residue motifs. We have applied this new method in the design of a four-fold symmetric eight-bladed beta-propeller and a two-fold symmetric double-psi beta-barrel core of RNA polymerase. These designs have been validated by X-ray crystallography that the experimentally determined structures closely matches the computationally design models.
|
| Academic Significance and Societal Importance of the Research Achievements |
私たちが開発した柔軟なバックボーン タンパク質設計法は、現在の最先端のタンパク質設計の可能性を拡張しました。 それは、新しいバイオ センサー、治療法、および診断法の開発に大きな可能性を示しています。 RNA ポリメラーゼの 2 重対称ダブル psi ベータ バレル コアの成功した設計は、現代の複雑なタンパク質は、祖先のより単純なタンパク質からの遺伝子重複、融合、および多様化イベントから進化した可能性があるという考えを支持しています。
|
Report
(4 results)
Research Products
(5 results)
