The role of intracellular organelles to regulate spatial organizations of the cell

• Project/Area Number: 18H02414
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43040:Biophysics-related
• Research Institution: National Institute of Genetics
• Principal Investigator: 木村 暁 国立遺伝学研究所, 遺伝メカニズム研究系, 教授 (10365447)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 線虫 / 細胞核 / 細胞質流動 / オルガネラ / 力学 / 数理モデル

Outline of Annual Research Achievements

本研究では、細胞核の細胞中央への移動や細胞質流動の発生におけるオルガネラの力学的な役割を定量的に明らかにすることを通じて、『真核生物の細胞内小器官（オルガネラ）が細胞内の大規模な空間再配置に必要な力学的要素として働いている』とするコンセプトを確立することを目指す。２０２０年度以降の主な進展は以下の通りである。
小胞体依存の細胞質流動が線虫C. elegansの受精後の細胞核のダイナミックな移動を駆動し、細胞核の位置によって決定づけられる細胞極性の確立を変えうることを見出し、査読付き英文国際誌Molecular Biology of the Cell誌における細胞と力に関する特集号に掲載された。また、細胞核の配置を規定するオルガネラである中心体の配置機構に関する新たな知見を得ることに成功した。これらの成果により細胞内の大規模な空間再配置におけるオルガネラの重要性を示すことができた。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Marine Biological Laboratory(米国)
• [Int'l Joint Research] Marine Biological Laboratory(米国)
• [Int'l Joint Research] Marine Biological Laboratory(米国)
• [Int'l Joint Research] Bayreuth University(ドイツ)
• [Journal Article] A mathematical model of kinetochore-microtubule attachment regulated by Aurora A activity gradient describes chromosome oscillation and correction of erroneous attachments (2021)
  • Author(s): Campos Medina Manuel Alejandro、Iemura Kenji、Kimura Akatsuki、Tanaka Kozo
  • Journal Title: Biomed. Res. Volume: 42 Issue: 5 Pages: 203-219
  • DOI: 10.2220/biomedres.42.203
  • NAID: 130008090723
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantifying the mobility of chromatin during embryogenesis: Nuclear size matters (2021)
  • Author(s): Aiya K. Yesbolatova, Ritsuko Arai, Takahiro Sakaue, Akatsuki Kimura
  • Journal Title: arXiv Volume: -
  • Open Access
• [Journal Article] The extra-embryonic space is a geometric constraint regulating cell arrangement in nematodes (2021)
  • Author(s): Seirin-Lee Sungrim、Kimura Akatsuki
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2021.06.12.448179
  • Open Access
• [Journal Article] Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo (2021)
  • Author(s): Torisawa Takayuki、Kimura Akatsuki
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2021.09.14.460385
  • Open Access
• [Journal Article] Cytoplasmic streaming drifts the polarity cue and enables posteriorization of theCaenorhabditis eleganszygote at the side opposite of sperm entry (2020)
  • Author(s): Kimura Kenji、Kimura Akatsuki
  • Journal Title: Molecular Biology of the Cell Volume: 31 Pages: 1765-1773
  • DOI: 10.1091/mbc.e20-01-0058
  • Peer Reviewed / Open Access
• [Journal Article] The Generation of Dynein Networks by Multi-Layered Regulation and Their Implication in Cell Division (2020)
  • Author(s): Takayuki Torisawa and Akatsuki Kimura
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 8 Pages: 1-13
  • DOI: 10.3389/fcell.2020.00022
  • Peer Reviewed / Open Access
• [Journal Article] Cytoplasmic streaming drifts the polarity cue and specifies the cell polarity in Caenorhabditis elegans zygotes (2019)
  • Author(s): Kimura Kenji、Kimura Akatsuki
  • Journal Title: bioRxiv Volume: - Pages: 887620-887620
  • DOI: 10.1101/2019.12.23.887620
  • Open Access
• [Journal Article] Choice between 1- and 2-furrow cytokinesis in Caenorhabditis elegans embryos with tripolar spindles (2019)
  • Author(s): Kondo Tomo、Kimura Akatsuki
  • Journal Title: Molecular Biology of the Cell Volume: 30
  • DOI: 10.1091/mbc.e19-01-0075
  • Peer Reviewed / Open Access
• [Presentation] Quantification and formulation of the effect of nuclear size on the mobility of chromatin in the C. elegans embryo. (2022)
  • Author(s): Akatsuki Kimura
  • Organizer: The 30th Hot Spring Harbor International Symposium on Chromatin Potential in Development and Differentiation
  • Int'l Joint Research / Invited
• [Presentation] Mechanics and self-organization of cytoplasmic steaming. (2021)
  • Author(s): Akatsuki Kimura
  • Organizer: RIMS Workshop on Mathematical Mechanobiology.
  • Int'l Joint Research / Invited
• [Presentation] Measurement of cellular forces bringing the nucleus to the cell center using the Centrifuge Polarization Microscope (CPM). (2020)
  • Author(s): Akatsuki Kimura
  • Organizer: The 58th Annual Meeting of the Biophysical Society Japan
• [Presentation] 線虫胚で”人工細胞核を造る”に向けた我々の取り組み (2019)
  • Author(s): 木村暁
  • Organizer: 第２回人工細胞核を創る会
• [Presentation] Architectonics of the cell, as a crossroad of physics and genetics (2019)
  • Author(s): Kimura A
  • Organizer: 第５７回日本生物物理学会年会
  • Invited
• [Presentation] 基礎生物学における新発見にデータ同化はどう有用か? (2019)
  • Author(s): 木村暁
  • Organizer: 科学基礎論学会2019年度秋の研究例会
  • Invited
• [Presentation] 遠心偏光顕微鏡 CPM を用いた細胞内の力の定量化 (2019)
  • Author(s): 木村暁
  • Organizer: 定量生物学の会北海道キャラバン2019
  • Invited
• [Presentation] Measurement of physical forces that position the nucleus at the cell center in C elegans. (2019)
  • Author(s): Kimura A
  • Organizer: Chromosome Dynamics 2019: An international symposium on chromatin and chromosome stability.
  • Int'l Joint Research
• [Presentation] 細胞の構造計算と力学測定：線虫 C. elegans 胚における細胞核配置 (2019)
  • Author(s): 木村暁
  • Organizer: 「細胞を創る」研究会12.0
• [Presentation] 「力」に着目して分子の集団が細胞内の核配置を制御する仕組みを紐解く (2019)
  • Author(s): 木村暁
  • Organizer: 第92回日本生化学会大会
• [Presentation] Physical forces behind the spatial organizations of the cell (2019)
  • Author(s): 木村暁
  • Organizer: 線虫研究の未来を創る会2019
• [Presentation] 遠心顕微鏡で細胞核を動かし、力を定量する (2019)
  • Author(s): 木村暁
  • Organizer: 定量生物学の会第９回年会
• [Presentation] Microtubule-dependent positioning of the nucleus in the C. elegans embryo (2018)
  • Author(s): Kimura A
  • Organizer: EMBO/EMBL Symposia: Microtubules: From Atoms to Complex Systems
  • Int'l Joint Research / Invited
• [Presentation] 二光子スピニングディスク顕微鏡を用いた線虫胚発生の低退色・低毒性イメージングの可能性 (2018)
  • Author(s): 木村暁
  • Organizer: 多次元生細胞イメージング研究会
  • Invited
• [Book] Quantitative Biology - A Practical Introduction. Learning Materials in Biosciences (2022)
  • Author(s): Akatsuki Kimura
  • Total Pages: 144
  • Publisher: Springer Nature Singapore
  • ISBN: 9789811650178
• [Book] 細胞建築学入門 (2019)
  • Author(s): 木村暁
  • Total Pages: 127
  • Publisher: 工学社
  • ISBN: 9784777520954
• [Book] 定量生物学 (2018)
  • Author(s): 小林 徹也
  • Total Pages: 250
  • Publisher: 化学同人
  • ISBN: 9784759817300
• [Remarks] 国立遺伝学研究所細胞建築（木村）研究室
  • URL: https://www.nig.ac.jp/nig/ja/research/organization-top/laboratories/kimura
• [Remarks] 国立遺伝学研究所細胞建築研究室
  • URL: http://cellarchlab.galaxy.bindcloud.jp/home_jp.html
• [Remarks] ２つに分裂するか、３つに分裂するか、それが問題だ
  • URL: https://www.nig.ac.jp/nig/ja/2019/04/research-highlights_ja/rh20190304.html