| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
To investigate whether highly sulfated heparan sulfate (HS) is present in early embryogenesis, we focused on the herpes simplex virus gD protein that binds to highly sulfated HS. gD requires sulfonation at position 3 to bind to HS, and the enzyme that catalyzes this is Hs3st3. The distribution of tagged gD in the intercellular spaces around the expressing cells was examined by immunostaining, and the results showed that gD was not observed in the gastrula embryo, but was detected in the neurula embryo, when the hs3st3 gene starts to be expressed. The fact that gD is actually secreted during the gastrula embryo was indicated by the result that gD bound around cells expressing antibodies to the tag. These data suggest that gD is a useful detection probe for highly sulfated HS.
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