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Study on the physiological roles of naturally occurring auxins in plants with distinct transport characteristics

Research Project

Project/Area Number 18H02457
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Kasahara Hiroyuki  東京農工大学, (連合)農学研究科(研究院), 教授 (00342767)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords植物ホルモン / オーキシン / 生合成 / 代謝 / フェニル酢酸 / インドール酢酸 / 成長制御 / フェニルアラニン / インドール-3-酢酸
Outline of Final Research Achievements

Auxin plays an essential role in plant growth and development. Phenylacetic acid (PAA) is an abundant natural auxin, but its biosynthesis and physiological roles remain elusive. In this study, we investigated the PAA biosynthesis pathway in Arabidopsis. We demonstrated that ADT genes can modulate the PAA levels and phenyl pyruvate is a key biosynthetic intermediate of PAA. CYP79A2 gene can modulate the PAA levels, but is not involved in the main PAA biosynthesis pathway. TAA1 homologs can catalyze the conversion of Phe to phenyl pyruvate in vitro, while they may not play important roles in PAA biosynthesis in plants.

Academic Significance and Societal Importance of the Research Achievements

本研究は、ユニークな移動特性を持つ天然オーキシンのフェニル酢酸(PAA)が、シロイヌナズナにおいてフェニルアラニンから主にフェニルピルビン酸を経由して合成されていることを示した。また、シロイヌナズナのインドール-3-酢酸(IAA)生合成に関わるTAA1やCYP79Bの遺伝子ホモログは、PAAの生合成には主として寄与していないことが示唆された。本研究の成果は、PAAの生合成を標的とした新たな除草剤や植物成長調整剤の開発に繋がる可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (22 results)

All 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Int'l Joint Research] University of California, San Diego(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Wageningen University & Research(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of California, San Diego(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Wageningen University & Research(オランダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Shanghai Normal University(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of California, San Diego(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] UDP-glucosyltransferase UGT84B1 regulates the levels of indole-3-acetic acid and phenylacetic acid in Arabidopsis2020

    • Author(s)
      Aoi Yuki、Hira Hayao、Hayakawa Yuya、Liu Hongquan、Fukui Kosuke、Dai Xinhua、Tanaka Keita、Hayashi Ken-ichiro、Zhao Yunde、Kasahara Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 532 Issue: 2 Pages: 244-250

    • DOI

      10.1016/j.bbrc.2020.08.026

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An evolutionarily primitive and distinct auxin metabolism in the lycophyte Selaginella moellendorffii2020

    • Author(s)
      Kaneko Shutaro、Cook Sam David、Aoi Yuki、Watanabe Akie、Hayashi Ken-Ichiro、Kasahara Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 10 Pages: 1724-1732

    • DOI

      10.1093/pcp/pcaa098

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in auxin metabolism2020

    • Author(s)
      Takubo Eiko、Kobayashi Makoto、Hirai Shoko、Aoi Yuki、Ge Chennan、Dai Xinhua、Fukui Kosuke、Hayashi Ken-ichiro、Zhao Yunde、Kasahara Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 527 Issue: 4 Pages: 1033-1038

    • DOI

      10.1016/j.bbrc.2020.05.031

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Arogenate dehydratases can modulate the levels of phenylacetic acid in Arabidopsis2020

    • Author(s)
      Aoi Yuki、Oikawa Akira、Sasaki Ryosuke、Huang Jirong、Hayashi Ken-ichiro、Kasahara Hiroyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 524 Issue: 1 Pages: 83-88

    • DOI

      10.1016/j.bbrc.2020.01.041

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] GH3 auxin-amido synthetases alter the ratio of indole-3-acetic acid and phenylacetic acid in Arabidopsis2019

    • Author(s)
      Aoi Yuki、Tanaka Keita、Cook Sam David、Hayashi Ken-Ichiro、Kasahara Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 3 Pages: 596-605

    • DOI

      10.1093/pcp/pcz223

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana2019

    • Author(s)
      Goh Tatsuaki、Toyokura Koichi、Yamaguchi Nobutoshi、Okamoto Yoshie、Uehara Takeo、Kaneko Shutaro、Takebayashi Yumiko、Kasahara Hiroyuki、Ikeyama Yoshifumi、Okushima Yoko、Nakajima Keiji、Mimura Tetsuro、Tasaka Masao、Fukaki Hidehiro
    • Journal Title

      New Phytologist

      Volume: 224 Issue: 2 Pages: 749-760

    • DOI

      10.1111/nph.16065

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls2019

    • Author(s)
      Mashiguchi Kiyoshi、Hisano Hiroshi、Takeda-Kamiya Noriko、Takebayashi Yumiko、Ariizumi Tohru、Gao Yangbin、Ezura Hiroshi、Sato Kazuhiro、Zhao Yunde、Hayashi Ken-ichiro、Kasahara Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 1 Pages: 29-37

    • DOI

      10.1093/pcp/pcy182

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シロイヌナズナのオーキシン濃度調節におけるUDP-グルコシル基転移酵素UGT84B1の役割2020

    • Author(s)
      比良隼、青井勇輝、早川雄也、Hongquan Liu、福井康佑、Xinhua Dai、田中慧太、林謙一郎、Yunde Zhao、笠原博幸
    • Organizer
      植物化学調節学会第55回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Coordination in the regulation of indole-3-acetic acid and phenylacetic acid metabolism in Arabidopsis2020

    • Author(s)
      Aoi Yuki、Tanaka Keita、Cook Sam David、Hayashi Ken-ichiro、Kasahara Hiroyuki
    • Organizer
      The 23rd International Conference on Plant Growth Substances
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biosynthesis and inactivation of two distinct auxins in plants2020

    • Author(s)
      Kasahara Hiroyuki
    • Organizer
      Tohoku Forum for Creativity Thematic Program 2010 Plant Stem Cells: Source of Plant Vitality Workshop2 Auxin and plant stem cells
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Differential gene expression analysis of Arabidopsis seedlings reveals potential involvement of 2-phenylacetic acid in hormone crosstalk and a role in plant microbe interactions2019

    • Author(s)
      Cook Sam David、Kimura Seisuke、Kasahara Hiroyuki
    • Organizer
      The 23rd International Conference on Plant Growth Substances
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IPA 経路を介したオーキシンの生合成が小葉シダ類イヌカタヒバの根の伸長や高温応答を制御する2019

    • Author(s)
      金子周太郎、Cook Sam David、林 謙一郎、笠原博幸
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 天然オーキシンPAAの生合成初期段階におけるフェニルアラニン合成酵素ADTの役割2019

    • Author(s)
      青井 勇輝、佐々木 亮介、及川 彰、林 謙一郎、笠原 博幸
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナにおける CYP79A2 依存的なフェニル酢酸生合成経路2019

    • Author(s)
      青井勇輝、武田紀子、竹林裕美子、林謙一郎、笠原博幸
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] New insights into auxin biosynthesis and inactivation in plants2019

    • Author(s)
      Aoi Yuki、Takeda Noriko、Takebayashi Yumiko、Hayashi Ken-ichiro、Kasahara Hiroyuki
    • Organizer
      Japan-Taiwan Plant Biology 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シロイヌナズナにおける非極性移動型オーキシン、フェニル酢酸のCYP79A2依存的な生合成経路の解析2018

    • Author(s)
      青井勇輝、武田紀子、竹林裕美子、林謙一郎、笠原博幸
    • Organizer
      植物化学調節学会第53回大会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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