| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Outline of Final Research Achievements |
Bacterial condensin localizes in the vicinity of the origin of chromosome replication. To investigate the molecular mechanism of condensin binding to rDNA, we conducted biochemical experiments on the topological binding of the E. coli condensin MukB to single-stranded DNA. The biochemical investigations revealed that MukB contains specific amino acid residues for binding to single-stranded DNA. These residues function as more stable single-stranded DNA domains when they are topologically bound to single-stranded DNA. Furthermore, during transcription, rDNA forms a single-stranded DNA domain downstream of its promoter, which facilitates the topological binding of bacterial condensin.
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