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縫合閉鎖から読み解く頭部骨数の減少進化

Research Project

Project/Area Number 18H02492
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionTokyo Medical and Dental University (2019-2020)
Musashino Art University (2018)

Principal Investigator

小薮 大輔  東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (60712510)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Granted (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords比較解剖学 / 頭蓋 / 形態学
Outline of Annual Research Achievements

本年度はマイクロフォーカスX線CT(島津製作所inspeXio SMX-90CT Plus)を用いてこれまでに申請者が収集してきた1028標本の胚標本の大量連続撮影をおこなった。本年度はコウモリ、ブタ、イヌ、ネコの撮影が主となった。Amiraソフトウェアを用いてこれらの三次元再構築作業をアルバイトを雇用しつつ遂行した。再構築作業を行ったセグメンテーションデータについて、骨格、筋、神経を中心とした比較解剖学的な観察記載を進めた。加えて、コウモリ、ブタの頭部組織切片を作成し、HNK1、MyoD、アセチル化チューブリンモノクローナル抗体による免疫染色とアザン染色を行った。組織切片をAmiraでアライメントを行ったのち三次元再構築し、胚における脳・神経・骨格・筋・血管の精確な三次元形態モデルを作成した。これによってコウモリとブタの胚生期における各パーツの立体的な相互配置、発生に伴う骨成長、骨の縫合閉鎖パターンを把握することに成功した。さらにIhh、 Ptch1、Sox9をマーカーとしてin situ ハイブリダイゼーションを行い、骨の縫合閉鎖の座標の記載を行った。これと並行して、カメおよびニワトリの胚を入手し、PFA固定、カルノア固定によって標本の固定を行った。また、予察的分析結果について、チェッコで開催された国際脊椎動物形態学会およびプーケットで開催された国際コウモリ類研究会議に参加して口頭発表を行い、関連分野の研究者と討議を行った。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

2月以降は新型コロナウイルスの影響で実験実施に支障も出始めたが、年間を通じては本課題に関わる論文発表も進めることができ、順調に進んでいる。

Strategy for Future Research Activity

CT撮像まだ完了していない標本は免疫染色とin situハイブリダイゼーションに供せないため、未撮影個体をできる限り早期に撮影を完了させる。撮影が済んだものは順次免疫染色とin situハイブリダイゼーションをおこなっていく。Amiraによる作業はかなり時間が掛かるものの単純作業であるため、ワークステーションの追加導入とともに学生アルバイトを雇用して作業効率向上を図りたい。新年度はin situハイブリダイゼーションが本格化する予定である。

Report

(2 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(16 results)

All 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (7 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Int'l Joint Research] ニューサウスウェールズ大学(オーストラリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] チュービンゲン大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ベトナム科学アカデミー生態資源研究所(ベトナム)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カリフォルニア大学ロサンゼルス校(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 3D model related to the publication: three-dimensional and histological observations on male genital organs of greater horseshoe bat, Rhinolophus ferrumequinum.2020

    • Author(s)
      Joon Hyuk Sohn, Junpei Kimura, Daisuke Koyabu
    • Journal Title

      Morphomuseum

      Volume: in press

    • DOI

      10.18563/journal.m3.113

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Non-invasive age estimation by cranial suture closure in Japanese sika deer (Cervus nippon).2019

    • Author(s)
      Oh, J.W., Minami, M.,Ikeda, S.,Takatsuki, S., Oonishi, N, Higuchi, N., Okada, A., Kimura, J., Koyabu, D
    • Journal Title

      Mammal Study

      Volume: 印刷中 Issue: 3 Pages: 147-147

    • DOI

      10.3106/ms2018-0065

    • NAID

      40021947477

    • ISSN
      1343-4152
    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Patterns and Constraints of Craniofacial Variation in Colobine Monkeys: Disentangling the Effects of Phylogeny, Allometry and Diet2019

    • Author(s)
      Ledevin Ronan、Koyabu Daisuke
    • Journal Title

      Evolutionary Biology

      Volume: 46 Pages: 14-34

    • DOI

      10.1007/s11692-019-09469-7

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Postcranial heterochrony, modularity, integration and disparity in the prenatal ossification in bats (Chiroptera)2019

    • Author(s)
      Lopez-Aguirre Camilo、Hand Suzanne J.、Koyabu Daisuke、Son Nguyen Truong、Wilson Laura A. B.
    • Journal Title

      BMC Evolutionary Biology

      Volume: 19 Pages: 75-75

    • DOI

      10.1186/s12862-019-1396-1

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prenatal allometric trajectories and the developmental basis of postcranial phenotypic diversity in bats (Chiroptera)2019

    • Author(s)
      Lopez-Aguirre Camilo、Hand Suzanne J.、Koyabu Daisuke、Son Nguyen Truong、Wilson Laura A. B.
    • Journal Title

      Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

      Volume: 332 Pages: 36-49

    • DOI

      10.1002/jez.b.22846

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biogeographic variation in skull morphology across the Kra Isthmus in dusky leaf monkeys.2018

    • Author(s)
      Ito, T., Koyabu, D.
    • Journal Title

      Journal of Zoological Systematics and Evolutionary Research

      Volume: in press Pages: 599-610

    • DOI

      10.1111/jzs.12229

    • NAID

      120006715529

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prenatal cranial bone development of Thomas’s horseshoe bat (Rhinolophus thomasi): with special reference to petrosal morphology.2018

    • Author(s)
      Nojiri, T., Werneburg, I., Son, N.T., Tu, V.T., Sasaki, T., Maekawa, Y., Koyabu, D.
    • Journal Title

      Journal of Morphology

      Volume: in press Pages: 809-827

    • DOI

      10.1002/jmor.20813

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Evolution of the temporal region in the early amniote skull2019

    • Author(s)
      Pascal Abel, Daisuke Koyabu, Ingmar Werneburg.
    • Organizer
      ドイツ古生物学会年次大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Prenatal Development of Echolocation-Related Traits in Bats and the Origin of Laryngeal Echolocation: Single Origin or Convergence?2019

    • Author(s)
      Koyabu D, Nojiri T.
    • Organizer
      国際脊椎動物形態学会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Morphogenetic Basis of Mammalian Flight: Allometric Trajectories and Ossification Heterochronies in Prenatal Skeletogenesis of Bats2019

    • Author(s)
      Lopez-Aguirre C, Wilson LAB, Koyabu D, Hand SJ.
    • Organizer
      国際脊椎動物形態学会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Does Prenatal Development Support a Single Origin of Laryngeal Echolocation in Bats?2019

    • Author(s)
      Nojiri T, Koyabu D.
    • Organizer
      国際脊椎動物形態学会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] On the Development of the Nasal Capsule and Turbinate Homology in Laurasiatherians, with Special Reference to Bats2019

    • Author(s)
      Ito K, Nojiri T, Koyabu D.
    • Organizer
      国際脊椎動物形態学会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2021-01-27  

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