Molecular and neural mechanism of memory regulation during sleep

• Project/Area Number: 18H02523
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 46010:Neuroscience-general-related
• Research Institution: Nagoya University
• Principal Investigator: 山中 章弘 名古屋大学, 環境医学研究所, 教授 (60323292)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Granted (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 睡眠覚醒 / メラニン凝集ホルモン / 視床下部 / 光遺伝学 / 遺伝子改変マウス / 睡眠 / オレキシン / 神経ペプチド / アデノ随伴ウイルスベクター / 化学遺伝学 / メラニン凝集ホルモン（MCH)神経 / 記憶 / 脳波筋電図 / レム睡眠 / nVista / 海馬

Outline of Annual Research Achievements

視床下部のメラニン凝集ホルモン産生神経(MCH)神経が、睡眠覚醒状態のどのタイミングで活性化されているのかについて、カルシウムインジケータであるGCaMP6とファイバーフォトメトリーを用いた記録を行った。その結果、MCH神経活動は、覚醒時に僅かに観察され、レム睡眠時に強い活動が見られるものの、ノンレム睡眠時にはほとんど活動が見られなかった。次に、超小型顕微鏡を用いた1細胞レベルでのMCH神経活動のイメージングによって、MCH神経には、覚醒時に活動するタイプ、レム睡眠時に活動するタイプ、覚醒時とレム睡眠時の両方で活動するタイプの3種類存在することが明らかになった。MCH神経活動を光遺伝学や化学遺伝学を用いて操作して、記憶を評価できる行動実験と組み合わせて、MCH神経活動と記憶の関係について検討を行った。その結果、MCH神経を活性化させると記憶が悪くなり、抑制すると記憶が良くなった。次に、3つのタイプのMCH神経のどのタイプが記憶の制御に関わっているのかを明らかにするために、脳波筋電図から睡眠覚醒状態をリアルタイムで判定しながら、MCH神経の活動を睡眠覚醒状態依存的に抑制した。その結果レム睡眠時だけでMCH神経活動を抑制した場合に記憶が良くなった。これらのことから、レム睡眠時に活動するMCH神経が記憶を悪くしていることが明らかになった(Izawa et al., Science 365(6459), pp.1308-1313, 2019)。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

光遺伝学、化学遺伝学などの神経活動操作を駆使してMCH神経活動がレム睡眠時に記憶の制御に関わっていることを明らかにし、これらの結果をまとめて、Science誌に報告することができた。

Strategy for Future Research Activity

今後はメラニン凝集ホルモン産生神経がレム睡眠時に記憶を制御する分子メカニズムについて、遺伝子改変マウスや、ウイルスベクターを用いて明らかにしていく。

Research Products

• [Int'l Joint Research] SRI International(米国)
• [Int'l Joint Research] Flinders University(オーストラリア)
• [Journal Article] Different roles of distinct serotonergic pathways in anxiety-like behavior, antidepressant-like, and anti-impulsive effects (2020)
  • Author(s): Ohmura Yu、Tsutsui-Kimura Iku、Sasamori Hitomi、Nebuka Mao、Nishitani Naoya、Tanaka Kenji F.、Yamanaka Akihiro、Yoshioka Mitsuhiro
  • Journal Title: Neuropharmacology. Volume: 167 Pages: 107703-107703
  • DOI: 10.1016/j.neuropharm.2019.107703
  • Peer Reviewed
• [Journal Article] Glutamatergic neurons in the medial prefrontal cortex mediate the formation and retrieval of cocaine-associated memories in mice. (2020)
  • Author(s): Zhang T, Yanagida J, Kamii H, Wada S, Domoto M, Sasase H, Deyama S, Takarada T, Hinoi E, Sakimura K, Yamanaka A, Maejima T, Mieda M, Sakurai T, Nishitani N, Nagayasu K, Kaneko S, Minami M, Kaneda K.
  • Journal Title: Addiction Biology Volume: Epub ahead of print Pages: 1-11
  • DOI: 10.1111/adb.12723
  • Peer Reviewed / Open Access
• [Journal Article] Acute restraint stress augments the rewarding memory of cocaine through activation of α1 adrenoceptors in the medial prefrontal cortex of mice. (2020)
  • Author(s): Wada S, Yanagida J, Sasase H, Zhang T, Li X, Kamii H, Domoto M, Deyama S, Hinoi E, Yamanaka A, Nishitani N, Nagayasu K, Kaneko S, Minami M, Kaneda K
  • Journal Title: Neuropharmacology Volume: 166 Pages: 107968-107968
  • DOI: 10.1016/j.neuropharm.2020.107968
  • Peer Reviewed
• [Journal Article] Involvement of supralemniscal nucleus (B9) 5-HT neuronal system in nociceptive processing: a fiber photometry study (2020)
  • Author(s): Moriya Shunpei、Yamashita Akira、Masukawa Daiki、Kambe Yuki、Sakaguchi Junichi、Setoyama Honami、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: Mol. brain Volume: 13 Pages: 14-14
  • DOI: 10.1186/s13041-020-0553-1
  • Peer Reviewed / Open Access
• [Journal Article] Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3. (2020)
  • Author(s): Imayoshi I, Tabuchi S, Matsumoto M, Kitano S, Miyachi H, Kageyama R, Yamanaka A
  • Journal Title: Sci Rep. Volume: 10(1) Pages: 3191-3191
  • DOI: 10.1038/s41598-020-59984-3
  • Peer Reviewed / Open Access
• [Journal Article] Behavioral characteristics of 5-HT2C receptor knockout mice: Locomotor activity, anxiety-, and fear memory-related behaviors (2020)
  • Author(s): Nebuka Mao、Ohmura Yu、Izawa Shuntaro、Bouchekioua Youcef、Nishitani Naoya、Yoshida Takayuki、Yoshioka Mitsuhiro
  • Journal Title: Behav Brain Res Volume: 379 Pages: 112394-112394
  • DOI: 10.1016/j.bbr.2019.112394
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion (2020)
  • Author(s): Nagai Yuma、Takayama Kaito、Nishitani Naoya、Andoh Chihiro、Koda Masashi、Shirakawa Hisashi、Nakagawa Takayuki、Nagayasu Kazuki、Yamanaka Akihiro、Kaneko Shuji
  • Journal Title: Int J Mol Sci Volume: 21 Pages: 2160-2160
  • DOI: 10.3390/ijms21062160
  • Peer Reviewed / Open Access
• [Journal Article] Dual orexin and MCH neuron-ablated mice display severe sleep attacks and cataplexy. (2020)
  • Author(s): Hung CJ, Ono D, Kilduff TS, Yamanaka A*
  • Journal Title: eLife Volume: in press
  • DOI: 10.7554/elife.54275
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Involvement of A13 dopaminergic neurons located in the zona incerta in nociceptive processing: a fiber photometry study (2020)
  • Author(s): Moriya Shunpei、Yamashita Akira、Masukawa Daiki、Setoyama Honami、Hwang Yunsu、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: Mol Brain Volume: 13 Pages: 60-60
  • DOI: 10.1186/s13041-020-00600-w
  • Peer Reviewed
• [Journal Article] Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice. (2020)
  • Author(s): Flanigan ME, Aleyasin H, Li L, Burnett CJ, Chan KL, LeClair KB, Lucas EK, Matikainen-Ankney B, Durand-de Cuttoli R, Takahashi A, Menard C, Pfau ML, Golden SA, Bouchard S, Calipari ES, Nestler EJ, DiLeone RJ, Yamanaka A, Huntley GW, Clem RL, Russo SJ
  • Journal Title: Nature Neuroscience Volume: in press
  • DOI: 10.1038/s41593-020-0617-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice (2019)
  • Author(s): Chowdhury S, Matsubara T, Miyazaki T, Ono D, Fukatsu N, Abe M, Sakimura K, Sudo Y, Yamanaka A*
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.44928
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice (2019)
  • Author(s): Chowdhury S, Hung CJ, Izawa S, Inutsuka A, Kawamura M, Kawashima T, Bito H, Imayoshi I, Abe M, Sakimura K, *Yamanaka A
  • Journal Title: eLife Volume: 8 Pages: 1-25
  • DOI: 10.7554/elife.44927
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acute nociceptive stimuli rapidly induce the activity of serotonin and noradrenalin neurons in the brain stem of awake mice (2019)
  • Author(s): Moriya Shunpei、Yamashita Akira、Nishi Ryusei、Ikoma Yoko、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: IBRO Rep Volume: 7 Pages: 1-9
  • DOI: 10.1016/j.ibror.2019.05.005
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR/Cas9-mediated in vivo gene editing reveals that neuronal 5-HT1A receptors in the dorsal raphe nucleus contribute to body temperature regulation in mice (2019)
  • Author(s): Nishitani Naoya、Ohmura Yu、Nagayasu Kazuki、Shibui Norihiro、Kaneko Shuji、Ohashi Akiko、Yoshida Takayuki、Yamanaka Akihiro、Yoshioka Mitsuhiro
  • Journal Title: Brain Res Volume: 1719 Pages: 243-252
  • DOI: 10.1016/j.brainres.2019.06.009
  • Peer Reviewed
• [Journal Article] Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination (2019)
  • Author(s): Yamanashi T, Maki M, Kojima K, Shibukawa A, Tsukamoto T, Chowdhury S, Yamanaka A, Takagi S, Sudo Y
  • Journal Title: Sci Rep Volume: 9(1) Pages: 7863-7863
  • DOI: 10.1038/s41598-019-44308-x
  • Peer Reviewed / Open Access
• [Journal Article] GABA in the suprachiasmatic nucleus refines circadian output rhythms in mice. (2019)
  • Author(s): Ono D, Honma KI, Yanagawa Y, Yamanaka A, Honma S
  • Journal Title: Communications Biology Volume: 2 Pages: 232-232
  • DOI: 10.1038/s42003-019-0483-6
  • Peer Reviewed / Open Access
• [Journal Article] Thin‐fibre receptors expressing acid‐sensing ion channel 3 contribute to muscular mechanical hypersensitivity after exercise (2019)
  • Author(s): Matsubara Takanori、Hayashi Koei、Wakatsuki Koji、Abe Masahiro、Ozaki Noriyuki、Yamanaka Akihiro、Mizumura Kazue、Taguchi Toru
  • Journal Title: Eur J Pain Volume: 23 Pages: 1801-1813
  • DOI: 10.1002/ejp.1454
  • Peer Reviewed
• [Journal Article] REM sleep-active MCH neurons are involved in forgetting hippocampus-dependent memories. (2019)
  • Author(s): Izawa S, Chowdhury S, Miyazaki T, Mukai Y, Ono D, Inoue R, Ohmura Y, Mizoguchi H, Kimura K, Yoshioka M, Terao A, Kilduff TS, Yamanaka A*
  • Journal Title: Science Volume: 365 Issue: 6459 Pages: 1308-1313
  • DOI: 10.1126/science.aax9238
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transgenic Archaerhodopsin-3 Expression in Hypocretin/Orexin Neurons Engenders Cellular Dysfunction and Features of Type 2 Narcolepsy. (2019)
  • Author(s): Williams RH, Tsunematsu T, Thomas AM, Bogyo K, Yamanaka A*. Kilduff TS*.
  • Journal Title: J Neurosci Volume: 39(47) Issue: 47 Pages: 9435-9452
  • DOI: 10.1523/jneurosci.0311-19.2019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mechanical allodynia induced by optogenetic sensory nerve excitation activates dopamine signaling and metabolism in medial nucleus accumbens. (2019)
  • Author(s): Sugiyama E, Kondo T, Kuzumaki N, Honda K, Yamanaka A, Narita M, Suematsu M, Sugiura Y.
  • Journal Title: Neurochemistry International Volume: 129 Pages: 104494-104494
  • DOI: 10.1016/j.neuint.2019.104494
  • Peer Reviewed / Open Access
• [Journal Article] Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo. (2019)
  • Author(s): Koizumi K, Inoue M, Chowdhury S, Bito H, Yamanaka A, Ishizuka T, Yawo H.
  • Journal Title: J Physiol Sci. Volume: 69(1) Issue: 1 Pages: 65-77
  • DOI: 10.1007/s12576-018-0618-4
  • NAID: 40021804699
  • Peer Reviewed
• [Journal Article] Large Timescale Interrogation of Neuronal Function by Fiberless Optogenetics Using Lanthanide Micro-particles. 26(4) : 1033-1043. e5. (2019)
  • Author(s): Miyazaki T, Chowdhury S, Yamashita T, Matsubara T, Yawo H, Yuasa H, Yamanaka A.
  • Journal Title: Cell Rep. Volume: 26 Issue: 4 Pages: 1033-1043
  • DOI: 10.1016/j.celrep.2019.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Preproenkephalin-expressing ventral pallidal neurons control inhibitory avoidance learning. (2019)
  • Author(s): Macpherson T, Mizoguchi H, Yamanaka A, Hikida T.
  • Journal Title: Neurochem Int Volume: 126 Pages: 11-18
  • DOI: 10.1016/j.neuint.2019.02.011
  • Peer Reviewed / Open Access
• [Journal Article] Upconversion Amplification through Dielectric Superlensing Modulation. (2019)
  • Author(s): Liang L, Teb D, Dinh ND, Chen W, Chen Q, Wu Y, Chowdhury S, Yamanaka A, Sum TC, Chen CH, Thakor N, All A, Liu X
  • Journal Title: Nat Commun Volume: 10(1) Issue: 1 Pages: 1391-1391
  • DOI: 10.1038/s41467-019-09345-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Opposing roles of dopamine receptor D1-and D2-expressing neurons in the anteromedial olfactory tubercle in acquisition of place preference in mice. (2019)
  • Author(s): Murata K, Kinoshita T, Fukazawa Y, Kobayashi K, Yamanaka A, Hikida T, Manabe H, Yamaguchi M.
  • Journal Title: Front Behav Neurosci Volume: 13 Pages: 50-50
  • DOI: 10.3389/fnbeh.2019.00050
  • Peer Reviewed / Open Access
• [Journal Article] Reward probability and timing uncertainty alter the effect of dorsal raphe serotonin neurons on patience. (2018)
  • Author(s): Miyazaki K, Miyazaki KW, Yamanaka A, Tokuda T, Tanaka KF, Doya K.
  • Journal Title: Nat Commun Volume: 9 Pages: 2048-2048
  • DOI: 10.1038/s41467-018-04496-y
  • Peer Reviewed / Open Access
• [Journal Article] Partial ablation of the orexin field induces a sub-narcoleptic phenotype in a conditional mouse model of orexin neurodegeneration. (2018)
  • Author(s): Black SW, Sun JD, Santiago P, Laihsu A, Kimura N, Yamanaka A, Bersot R, Humphries PS
  • Journal Title: Sleep Volume: 41(9) Issue: 9
  • DOI: 10.1093/sleep/zsy116
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Inactivation of serotonergic neurons in the rostral medullary raphe attenuates stress-induced tachypnea and tachycardia in mice. (2018)
  • Author(s): Ikoma Y, Kusumoto-Yoshida I, Yamanaka A, Ootsuka Y, Kuwaki T.
  • Journal Title: Front Physiol Volume: 9 Pages: 1-16
  • DOI: 10.3389/fphys.2018.00832
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acute aversive stimuli rapidly increase the activity of ventral tegmental area dopamine neurons in awake mice. (2018)
  • Author(s): Moriya S, Yamashita A. Kawashima S, Nishi R, Yamanaka A, Kuwaki T.
  • Journal Title: Neuroscience Volume: 386 Pages: 16-23
  • DOI: 10.1016/j.neuroscience.2018.06.027
  • Peer Reviewed / Open Access
• [Journal Article] Parallel arousal pathways in the lateral hypothalamus. (2018)
  • Author(s): Heiss JE, Yamanaka A, Kilduff TS.
  • Journal Title: eNeuro Volume: 5(4) Pages: 0228-18
  • DOI: 10.1523/eneuro.0228-18.2018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Calcium transient dynamics of neural ensembles in the primary motor cortex of naturally behaving monkeys. (2018)
  • Author(s): Kondo T, Saito R, Otaka M, Yoshino-Saito K, Yamanaka A, Yamamori T, Watakabe A, Mizukami H, Schnitzer MJ, Tanaka KF. Ushiba J. Okano H.
  • Journal Title: Cell Rep Volume: 24(8) Issue: 8 Pages: 2191-2195
  • DOI: 10.1016/j.celrep.2018.07.057
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Opiates increase the number of hypocretin-producing cells in human and mouse brain and reverse cataplexy in a mouse model of narcolepsy. (2018)
  • Author(s): Thannickal TC, John J, Shan L, Swaab DF, Wu MF, Ramanathan L, McGregor R, Chew KT, Cornford M, Yamanaka A, Inutsuka A, Fronczek R, Lammers GJ, Worley PF, Siegel JM.
  • Journal Title: Sci Transl Med Volume: 10 Pages: 447-447
  • DOI: 10.1126/scitranslmed.aao4953
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Neuronal sirtl regulates macronutrient-based diet selection through fgf21 and oxytocin signalling in mice. (2018)
  • Author(s): Matsui S, Sasaki T, Kohno D, Yaku K, Inutsuka A, Yokota-Hashimoto H, Kikuchi O. Suga T. Kobayashi M. Yamanaka A, Harada A, Nakagawa T. Onaka T, Kitamura T.
  • Journal Title: Nat Commun Volume: 9 Issue: 1 Pages: 1-17
  • DOI: 10.1038/s41467-018-07033-z
  • Peer Reviewed / Open Access
• [Presentation] 視床下部神経による睡眠覚醒と記憶の制御 (2020)
  • Author(s): 山中章弘
  • Organizer: 第218回精神科医会及び第174回学術講演会
  • Invited
• [Presentation] オレキシンとは？睡眠覚醒調節における役割について (2020)
  • Author(s): 山中章弘
  • Organizer: 東区医師会学術講演会
  • Invited
• [Presentation] Functional interaction between the hypothalamus, septum and hippocampus to regulate sleep and memory (2019)
  • Author(s): Yamanaka A
  • Organizer: NUS Physiology Research Seminar
  • Int'l Joint Research / Invited
• [Presentation] Role of REM sleep on memory (2019)
  • Author(s): Yamanaka A
  • Organizer: Cold Spring Harbor Asia Neurobiology of Behavior & Neuropsychiatric Disorders
  • Int'l Joint Research / Invited
• [Presentation] REM sleep active MCH neurons are involved in forgetting hippocampus-dependent memories (2019)
  • Author(s): Yamanaka A, Izawa S
  • Organizer: IBRO 2019
  • Int'l Joint Research / Invited
• [Presentation] MCH neurons impaired memories during REM sleep (2019)
  • Author(s): Yamanaka A
  • Organizer: 2019 Chinese Sleep Research Society (CSRS) Annual Academic Conference
  • Int'l Joint Research / Invited
• [Presentation] REM sleep active MCH neurons are involved in forgetting hippocampus-dependent memories (2019)
  • Author(s): Yamanaka A
  • Organizer: 6th Congress of Asian College of Neuropsychopharmacology
  • Int'l Joint Research
• [Presentation] 睡眠覚醒調節と記憶の制御に関わる神経の活動記録と操作 (2019)
  • Author(s): 山中章弘
  • Organizer: 平成30年度生命融合科学教育部シンポジウム 神経科学が解き明かす動物の行動：機能の変容と疾患
  • Invited
• [Presentation] 睡眠覚醒調節神経の機能的同定 (2019)
  • Author(s): 山中章弘
  • Organizer: 第92回日本薬理学会年会
  • Invited
• [Presentation] Adeno-associated virus vector micro injection into mice brain to reveal function of neural circuitry involved in the regulation of sleep/wakefulness. (2019)
  • Author(s): Yamanaka A
  • Organizer: FAOPS2019
  • Int'l Joint Research / Invited
• [Presentation] Regulation of sleep and memory (2018)
  • Author(s): Yamanaka A
  • Organizer: 第10回光操作研究会・第2回脳情報動態合同国際シンポジウムJoint symposium of 10th Optogenetics Research Conference and Second International Symposium on Brain Information Dynamics 2018
  • Int'l Joint Research / Invited
• [Presentation] Dissociating orexin-dependent and -independent functions of orexin neurons using novel orexin-Flp knock-in mice (2018)
  • Author(s): Yamanaka A
  • Organizer: 7th International Symposium on Narcolepsy
  • Int'l Joint Research / Invited
• [Presentation] Sleep/wakefulness analysis of orexin and MCH neurons double ablated mice (2018)
  • Author(s): Hung CJ, Ono D, Yamanaka A
  • Organizer: 7th International Symposium on Narcolepsy
  • Int'l Joint Research / Invited
• [Presentation] Orexin and MCH neurons double ablation mice. Isolation of effect of orexin using orexin-Flippase mice. (2018)
  • Author(s): Yamanaka A
  • Organizer: 7th International Symposium on Narcolepsy
  • Int'l Joint Research / Invited
• [Presentation] Neural activity manipulation and behavior control by using fiber-less optogenetics (2018)
  • Author(s): Yamanaka A
  • Organizer: National University of Singapore, Department of Chemistry, Research Seminar
  • Int'l Joint Research / Invited
• [Presentation] Regulatory mechanism of sleep/wakefulness and memory by the hypothalamic neurons (2018)
  • Author(s): 山中章弘
  • Organizer: 島根大学セミナー
  • Invited
• [Presentation] 睡眠覚醒調節、痛み調節におけるオレキシンの役割 (2018)
  • Author(s): 山中章弘
  • Organizer: 第12回日本緩和医療薬学会年会
  • Invited
• [Presentation] 睡眠覚醒調節におけるオレキシンの役割 (2018)
  • Author(s): 山中章弘
  • Organizer: 第41回日本神経科学大会 ランチョンセミナー10
  • Invited
• [Presentation] Role of cholinergic neurons in the septum on the regulation of sleep and memory (2018)
  • Author(s): 山中章弘、宮﨑杜夫
  • Organizer: 第41回日本神経科学大会 シンポジウム： 神経修飾物質による脳状態制御： 先進的実験と計算論的アプローチを結ぶ
  • Invited
• [Presentation] アップコンバージョンを用いたファイバーレス光遺伝学の開発 (2018)
  • Author(s): 宮﨑杜夫、Chowdhury Srikanta、山下貴之、八尾 寛、湯浅英哉、山中章弘
  • Organizer: 第56回日本生物物理学会年会
  • Invited
• [Presentation] Study of regulatory mechanism of sleep/wakefulness and memory by optogenetical neural activity manipulation (2018)
  • Author(s): 山中章弘
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] 内側中核アセチルコリン作動性神経による睡眠と記憶の制御 (2018)
  • Author(s): 山中章弘
  • Organizer: 記憶研究会2018 記憶・学習の基盤機構と回路研究の新展開へのアプローチ
  • Invited
• [Presentation] 睡眠と記憶を制御する神経活動の操作と記録 (2018)
  • Author(s): 山中章弘
  • Organizer: 第８回生理学研究所・名古屋大学医学系研究科合同シンポジウム
  • Invited
• [Presentation] 睡眠と記憶を制御する神経活動の操作と記録 (2018)
  • Author(s): 山中章弘
  • Organizer: 星薬科大学セミナー
  • Invited
• [Presentation] ファイバーレス光遺伝学を用いた睡眠覚醒と記憶制御のメカニズム解明 (2018)
  • Author(s): 山中章弘
  • Organizer: 第三回ルミノジェネティクス研究会
  • Invited
• [Presentation] 生活習慣病と睡眠覚醒とのただならぬ関係 (2018)
  • Author(s): 山中章弘
  • Organizer: 第3回Cortin Conference
  • Invited
• [Presentation] 腹側被蓋野(VTA)のGABA作動性神経はノンレム睡眠を調節する (2018)
  • Author(s): 山中章弘
  • Organizer: 平成30年度脳研究所共同利用共同研究合同セミナー
  • Invited
• [Remarks] 浅い眠りで記憶が消去される仕組みを解明
  • URL: http://www.riem.nagoya-u.ac.jp/4/drof1/nr/files/Sci_190920.pdf