• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of transmission and plastcity at mammalian central synapses.

Research Project

Project/Area Number 18H02530
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionDoshisha University

Principal Investigator

Sakaba Takeshi  同志社大学, 脳科学研究科, 教授 (80609511)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywordsシナプス / 神経 / 可塑性
Outline of Final Research Achievements

In order to understand the cellular mechanisms of transmission and plasticity at mammalian central synapses, we have applied simultaneous pre- and postsynaptic patch clamp to hippocampal mossy fiber synapses. We found that the intra-cellular Ca sensitivity for transmitter release was similar to the one at other synapse types, but the local Ca concentration at the transmitter release site was much lower, explaining low release probability of release at mossy fiber synapses. In addition, we found that cAMP-induced potentiation of transmitter release, important for short- and long-term plasticity, was mediated by accumulation of Ca channels near release sites, thereby increasing the local Ca concentration at the release site.

Academic Significance and Societal Importance of the Research Achievements

本研究は、哺乳類中枢シナプスのシナプス前後部記録によって、厳密に伝達特性を測定し、それによって、伝達、可塑性の分子細胞メカニズムを明らかにした。海馬苔状線維シナプスではCa-伝達物質放出連関が特殊であり、それが伝達効率の低さの主要因となっていること、また可塑性因子であるcAMPによってCaチャネルクラスターが拡大し、それによって伝達物質放出量増大によってシナプス増強などの可塑性がおこることが明らかになった。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(16 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Remarks (2 results)

  • [Int'l Joint Research] ベルリン自由大学(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses2021

    • Author(s)
      Fukaya Ryota、Maglione Marta、Sigrist Stephan J.、Sakaba Takeshi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118

    • DOI

      10.1073/pnas.2016754118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Direct imaging of rapid tethering of synaptic vesicles accompanying exocytosis at a fast central synapse2020

    • Author(s)
      Miki Takafumi、Midorikawa Mitsuharu、Sakaba Takeshi
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 117 Pages: 14493-14502

    • DOI

      10.1073/pnas.2000265117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission2020

    • Author(s)
      Jaepel Maria、Gerth Fabian、Sakaba Takeshi、Bacetic Jelena、Yao Lijun、Koo Seong-Joo、Maritzen Tanja、Freund Christian、Haucke Volker
    • Journal Title

      Cell Reports

      Volume: 30

    • DOI

      10.1016/j.celrep.2019.12.035

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developmental changes in the excitatory short‐term plasticity at input synapses in the rat inferior colliculus2019

    • Author(s)
      Kitagawa Mako、Sakaba Takeshi
    • Journal Title

      European Journal of Neuroscience

      Volume: 50 Pages: 2830-2846

    • DOI

      10.1111/ejn.14422

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ca-dependence of synaptic vesicle exocytosis and endocytosis at the hippocampal mossy fibre terminal2019

    • Author(s)
      Miyano R., Miki T., Sakaba T.
    • Journal Title

      The Journal of Physiology

      Volume: 597 Pages: 4373-4386

    • DOI

      10.1113/jp278040

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hapln4/Bral2 is a selective regulator for formation and transmission of GABAergic synapses between Purkinje and deep cerebellar nuclei neurons2018

    • Author(s)
      Edamatsu Midori、Miyano Rinako、Fujikawa Atsushi、Fujii Fuminari、Hori Tetsuya、Sakaba Takeshi、Oohashi Toshitaka
    • Journal Title

      J Neurochem

      Volume: 147 Pages: 748-763

    • DOI

      10.1111/jnc.14571

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optimal dissection of a model circuit2018

    • Author(s)
      Sakaba Takeshi
    • Journal Title

      J Physiol

      Volume: 596 Pages: 4807-4808

    • DOI

      10.1113/jp276895

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Comparison of the transmitter release properties between the calyx of Held synapse and mossy fiber-CA3 synapse.2019

    • Author(s)
      Sakaba T
    • Organizer
      Quantitative Aspects of Membrane Fusion and Fission, Biophysical Society thematic meeting, Padova,Italy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Kinetics of transmitter release at hippocampal mossy fiber synapses2019

    • Author(s)
      Sakaba T
    • Organizer
      日米脳:シナプス―回路シンポジウム、御殿場
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biophysics of transmitter release at the mossy fiber synapse.2019

    • Author(s)
      Sakaba T
    • Organizer
      BMI seminar, EPFL Lausanne, Swiss
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Ca-secretion coupling at mammalian CNS2019

    • Author(s)
      Sakaba T
    • Organizer
      FAOPS meeting, 神戸
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of exocytosis by cAMP at hippocampal mossy fiber terminals.2018

    • Author(s)
      Sakaba T
    • Organizer
      日本神経科学学会 神戸
    • Related Report
      2018 Annual Research Report
  • [Presentation] Biophysics of Neurotransmitter Release at Central Synapses.2018

    • Author(s)
      Sakaba T
    • Organizer
      n NIH Neuroscience seminar series,National Institute for Health, USA, Invited talk,
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Remarks] http://brainscience.doshisha.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] NIH neuroscience seminar series (2018.12)

    • URL

      https://videocast.nih.gov/summary.asp?Live=28085&bhcp=1

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi