Regulatory mechanisms for signals on cell membrane and under microenvironment by complex of glycosphingolipids and membrane molecules

• Project/Area Number: 18H02628
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 49010:Pathological biochemistry-related
• Research Institution: Chubu University
• Principal Investigator: Furukawa Koichi 中部大学, 生命健康科学部, 特任教授 (80211530)
• Co-Investigator(Kenkyū-buntansha): 大海 雄介 中部大学, 生命健康科学部, 助教 (10584758) ; 古川 圭子 中部大学, 生命健康科学部, 教授 (50260732)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 脂質ラフト / スフィンゴ糖脂質 / ミクロドメイン / クラスター / 複合体 / 細胞外小胞 / 微小環境 / セラミド、 / セラミド / 膜分子 / シグナル / 細胞外顆粒

Outline of Final Research Achievements

In order to analyze behavior and function of membrane lipid rafts where glycolipids localize, we tried to identify their associating molecules and to investigate the complex formation of them and regulatory function of cells. Then, we analyzed the relationship of them with extracellular vesicles (EVs). First of all, we identified molecules associated with cancer-associated glycolipids using EMARS/MS method, and revealed their complex formation. Furthermore, we examined chemical composition of lipid rafts and EVs, and performed proteomics analysis with MS, leading to elucidation of important roles of lipid rafts in the EV generation, secretion and incorporation. Analyses of mechanisms for signal regulation with complexes of glycosphingolipids and membrane molecules at lipid rafts, and of regulatory function of microenvironments with EVs revealed that EVs as well as rafts enhance malignant properties of cancers and induce activation of malignant signals.

Academic Significance and Societal Importance of the Research Achievements

種々の細胞、特にがん細胞の膜上における糖脂質の役割に関して具体的な作用機構が明らかになった。糖脂質と会合する膜分子が同定され、膜のミクロドメインである脂質ラフトにおいて複合体を形成して細胞シグナルの制御に協働的に作用することが示された。同時に、細胞が分泌する細胞外小胞(EV)の生成・分泌・作用機構に対する脂質ラフトの深い関与が明らかになり、癌の微小環境制御機能における癌関連糖脂質と会合膜分子との複合体の重要性が初めて明らかにされたことは、学術的な意義が極めて大きい。同時に、癌関連糖鎖を標的にした癌治療・予防法を考案する上で示唆的な内容が多く含まれており、癌治療のあり方に対する意義は甚大である。

Research Products

• [Int'l Joint Research] Chittagong University/Dep Biochem Mol Biol(バングラデシュ)
• [Int'l Joint Research] Harvard University/Dep Neurol. Children Hosp.(米国)
• [Journal Article] Ganglioside GD2 Enhances the Malignant Phenotypes of Melanoma Cells by Cooperating with Integrins. (2022)
  • Author(s): Yesmin, F., Bhuiyan, R.H., Ohmi, Y., Yamamoto, S., Kaneko, K., Ohkawa, Y., Zhang, P., Hamamura, K., Cheung, N-K.V., Kotani, N., Honke, K., Okajima, T., Kambe, M., Tajima, O., Furukawa, K., Furukawa, K.
  • Journal Title: Int J Mol Sci. Volume: 23 Pages: 423-423
  • DOI: 10.3390/ijms23010423
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinguishing Functional Exosomes and Other DNA-containing Extracellular Vesicles Based on the Difference in Strength of Negative Membrane Charge (2022)
  • Author(s): Seo, Naohiro; Nakamura, Junko; Kaneda, Tsuguhiro; Tateno, Hiroaki; Shimoda, Asako; Ichiki, Takanori; Furukawa, Koichi; Hirabayashi, Jun; Kazunari, Akiyoshi; Shiku, Hiroshi
  • Journal Title: Journal of Extracellular Vesicles Volume: 11 Pages: 12205-12205
  • DOI: 10.1002/jev2.12205
  • Peer Reviewed
• [Journal Article] Signaling domains of cancer-associated glycolipids. Tribute to Professor Sen-itiroh Hakomori (2022)
  • Author(s): Furukawa, K., Ohmi, Y., Hamamura, K., Kondo, Y., Ohkawa, Y., Kaneko, K., Hashimoto, N., Farhana, Y., Bhuiyan, R.H., Tajima, O., Furukawa, K.
  • Journal Title: Glycoconj. J. Volume: 39 Pages: 145-155
  • DOI: 10.1007/s10719-022-10051-1
  • Peer Reviewed / Open Access
• [Journal Article] 糖鎖による抗体の機能調節と膠原病の病態 (2022)
  • Author(s): 大海雄介, 古川鋼一
  • Journal Title: 皮膚科 Volume: 1 Pages: 39-47
• [Journal Article] Majority of alpha2,6-sialylated glycans in adult mouse brain exist in O-glycans: SALSA-MS analysis for knockout mice of alpha2,6-sialyltransferase genes (2021)
  • Author(s): Ohmi Y, Nishikaze T, Kitaura Y, Ito T, Yamamoto S, Sugiyama F, Matsuyama M, Takahashi Y, Takeda A, Kawahara T, Okajima T, Furukawa K, Furukawa K
  • Journal Title: Glycobiology Volume: 105 Pages: 1-14
  • DOI: 10.1093/glycob/cwaa105
  • Peer Reviewed / Open Access
• [Journal Article] Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling (2021)
  • Author(s): Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K
  • Journal Title: Anticancer Research Volume: 41 Pages: 1821-1830
  • DOI: 10.21873/anticanres.14948
  • Peer Reviewed / Open Access
• [Journal Article] Lack of GD3 synthase (St8sia1) attenuates malignant properties of gliomas in genetically engineered mouse model. (2021)
  • Author(s): Ohkawa, Y., Zhang, P., Momota, H., Kato, A., Hashimoto, N., Ohmi, Y., Bhuiyan, R.H., Natsume, A., Wakabayashi, T., Furukawa, K, Furukawa K.
  • Journal Title: Cancer Science Volume: 112 Pages: 3756-3768
  • DOI: 10.1111/cas.15032
  • Peer Reviewed / Open Access
• [Journal Article] St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity (2021)
  • Author(s): Zhang, P., Ohkawa, Y., Yamamoto, S., Momota, H., Kato, A., Kaneko, K., Natsume, A., Farhana, Y., Ohmi, Y., Okajima, T., Bhuiyan, R.H., Wakabayashi, T., Furukawa, K., Furukawa, K.
  • Journal Title: Nagoya Journal of Medical Science Volume: 83 Issue: 3 Pages: 535-549
  • DOI: 10.18999/nagjms.83.3.535
  • NAID: 120007147350
  • ISSN: 0027-7622
  • URL: https://nagoya.repo.nii.ac.jp/records/2001444
  • Peer Reviewed / Open Access
• [Journal Article] Contribution of glucosylceramide synthase to the proliferation of mouse osteoblasts (2021)
  • Author(s): Mishima, Y., Hamamura, K., Kato, H., Furukawa, K., Tashima, Y., Okajima, T., Kondo, H., Sato, T., Miyazawa, K., Goto, S., Togari, A.
  • Journal Title: In Vivo Volume: 35 Pages: 3111-3123
  • DOI: 10.21873/invivo.12606
  • Peer Reviewed / Open Access
• [Journal Article] A nontoxigenic form of Shiga toxin 2 suppresses the production of amyloid b by altering the intracellular transport of amyloid precursor protein through its receptor-binding B-subunit (2021)
  • Author(s): Waka Sato, Miho Watanabe-Takahashi, Takashi Hamabata, Koichi Furukawa, Satoru Funamoto, Kiyotaka Nishikawa
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 557 Pages: 247-253
  • DOI: 10.1016/j.bbrc.2021.04.015
  • Peer Reviewed / Open Access
• [Journal Article] Furukawa K. (2020)
  • Author(s): Furukawa K.
  • Journal Title: Int J Mol Sci. Volume: 21
  • DOI: 10.3390/ijms21186552
  • Peer Reviewed / Open Access
• [Journal Article] Aminoglycosides are efficient reagents to induce readthrough of premature termination codon in mutant B4GALNT1 genes found in families of hereditary spastic paraplegia (2020)
  • Author(s): Yesmin F, Bhuiyan RH, Ohmi Y, Ohkawa Y, Tajima O, Okajima T, Furukawa K, Furukawa K.
  • Journal Title: The Journal of Biochemistry Volume: 168 Pages: 103-112
  • DOI: 10.1093/jb/mvaa041
  • NAID: 40022308727
  • Peer Reviewed / Open Access
• [Journal Article] SSEA-3 and 4 are not essential for the induction or properties of mouse iPS cells (2020)
  • Author(s): Hamamura Kazunori、Hotta Hiroshi、Murakumo Yoshiki、Shibuya Hidenobu、Kondo Yuji、Furukawa Koichi
  • Journal Title: J Oral Sci Volume: 62 Issue: 4 Pages: 393-396
  • DOI: 10.2334/josnusd.19-0513
  • NAID: 130007908552
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed / Open Access
• [Journal Article] Novel molecular mechanisms for roles of gangliosides in the nervous system elucidated by genetic engineering. (2020)
  • Author(s): Furukawa, K., Ohmi, Y., Yesmin, F., Tajima, O., Kondo, Y., Pu Zhang, P., Hashimoto, N., Ohkawa, Y., Bhuiyan, R.H., Furukawa, K.
  • Journal Title: Int J Mol Sci. Volume: 21 Pages: 1906-1906
  • DOI: 10.3390/ijms21061906
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Elucidation of Pathological Mechanisms for Involvement of Gangliosides in Intractable Diseases (2020)
  • Author(s): Y Ohmi, K Furukawa, K Furukawa
  • Journal Title: TIGG Volume: 32 Issue: 187 Pages: E81-E86
  • DOI: 10.4052/tigg.2011.2e 10.4052/tigg.2011.2E
  • NAID: 130007846166
  • ISSN: 0915-7352, 1883-2113
  • Peer Reviewed / Open Access
• [Journal Article] Loss of enzyme activity of mutated B4GALNT1 gene products found in patients with hereditary spastic paraplegia induces relatively mild neurological disorders: Similarity with phenotypes of B4galnt1 knockout mice. (2019)
  • Author(s): Robiul H. Bhuiyan, Yuhsuke Ohmi, Yuki Ohkawa, Pu Zhang, Maiko Takano, Noboru Hashimoto, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Journal Title: Neuroscienc Volume: 397 Pages: 94-106
  • DOI: 10.1016/j.neuroscience.2018.11.034
  • Peer Reviewed
• [Journal Article] Anti-GD2 CAR T cells could prove transformative for H3-K27M+ diffuse midline gliomas (2019)
  • Author(s): Koichi Furukawa, Yuhsuke Ohmi, Keiko Furukawa
  • Journal Title: Translational Cancer Research Volume: 8(2) Pages: 87-93
  • DOI: 10.21037/tcr.2018.08.21
  • Peer Reviewed / Open Access
• [Journal Article] New era of research on cancer‐associated glycosphingolipids. (2019)
  • Author(s): Koichi Furukawa, Yuhsuke Ohmi, Yuki Ohkawa, Robiul H. Bhuiyan, Pu Zhang, Orie Tajima, Noboru Hashimoto, Kazunori Hamamura, Keiko Furukawa.
  • Journal Title: Cancer Sci. Volume: - Pages: 1544-1551
  • DOI: 10.1111/cas.14005
  • NAID: 120006939607
  • Peer Reviewed / Open Access
• [Journal Article] Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells. (2018)
  • Author(s): Tsuchida A, Senda M, Ito A, Saito S, Kiso M, Ando T, Harduin-Lepers A, Matsuda A, Furukawa K, Furukawa K.
  • Journal Title: Sci. Rep. Volume: 8 Pages: 1-15
  • DOI: 10.1038/s41598-018-25521-6
  • NAID: 120006941172
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of malignant properties of human glioma cells by ganglioside GD3/GD2. (2018)
  • Author(s): Taiji Iwasawa, Pu Zhang, Yuki Ohkawa, Hiroyuki Momota, Toshihiko Wakabayashi, Yuhsuke Ohmi, Robiul H. Bhuiyan, Keiko Furukawa, Koichi Furukawa
  • Journal Title: Int J Oncol. Volume: 52 Pages: 1255-1266
  • DOI: 10.3892/ijo.2018.4266
  • Peer Reviewed
• [Journal Article] ASC amino acid transporter 2, defined by enzyme-mediated activation of radical sources, enhances malignancy of GD2-positive small-cell lung cancer. (2018)
  • Author(s): Nobutoshi Esaki, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Tsuda, Yuhsuke Ohmi, Robiul H. Bhuiyan, Norihiro Kotani, Koichi Honke, Atsushi Enomoto, Masahide Takahashi, Keiko Furukawa, Koichi Furukawa.
  • Journal Title: Cancer Scienc Volume: 109 Pages: 141-153
  • DOI: 10.1111/cas.13448
  • Peer Reviewed / Open Access
• [Journal Article] Lactosylceramide synthase, encoded by β4-galactosylteransferase-5 and -6, is pivotal for neuronal generation and myelin formation in mice (2018)
  • Author(s): Toru Yoshihara, Hiroyuki Satake, Toshikazu Nishie, Nozomu Okino, Toshihisa Hatta, Hiroki Otani, Hiroshi Suzuki, Kazushi Sugihara, Eikichi Kamimura, Noriyo Tokuda, Keiko Furukawa, Koichi Fururkawa, Makoto Ito, and Masahide Asano
  • Journal Title: Plos Genetics Volume: 14
  • Peer Reviewed
• [Journal Article] Gangliosides in inflammation and neurodegeneration. (2018)
  • Author(s): Furukawa K, Ohmi Y, Tajima O, Ohkawa Y, Kondo Y, Shuting J, Hashimoto N, Furukawa K
  • Journal Title: Prog. Mol. Biol. Transl. Sci. Volume: 156 Pages: 265-287
  • DOI: 10.1016/bs.pmbts.2018.01.009
  • Peer Reviewed
• [Journal Article] Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking (2018)
  • Author(s): Kondo, Y., Fu, J., Wang, H., Hoover, C., McDaniel, M., Steet, R., Patra, D., Song, J., Pollard, J., Cathey, S., Yago, T., Wiley, G., Macwana, S., Guthridge, J., McGee, S., Li, S., Griffin, C., Furukawa, K., James, J.A., Ruan, C., McEver, R.P., Wierenga, K., Gaffney, P., Xia, L.
  • Journal Title: J Clin Invest Insight Volume: 3
  • DOI: 10.1172/jci.insight.121596
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genome-wide CRISPR screens for Shiga toxins and Ricin reveal Golgi proteins critical for glycosylation. (2018)
  • Author(s): Songhai Tian, Khaja Muneeruddin, Mei Yuk Choi, Liang Tao, Robiul H. Bhuiyan, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa, Sebastian Boland, Scott A. Shaffer, Rosalyn M. Adams, Min Dong
  • Journal Title: PLoS Biol. Volume: 16(11) Pages: 1-31
  • DOI: 10.1371/journal.pbio.2006951
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Differential roles of gangliosides in malignant properties of melanomas. (2018)
  • Author(s): Ohmi Y, Kambe M, Ohkawa Y, Hamamura K, Tajima O, Takeuchi R, Furukawa K, Furukawa K
  • Journal Title: PLOS One Volume: 13(11)
  • DOI: 10.1371/journal.pone.0206881
  • Peer Reviewed / Open Access
• [Presentation] 新規GD2特異的CAR遺伝子導入アロgamma/delta-T 細胞の開発 (2021)
  • Author(s): 藤原 弘, 赤堀泰, 三輪啓志, 王立楠,日向千咲, 大海雄介, 宮原慶裕, 田中義正, 古川圭子, 古川鋼一, 珠玖 洋
  • Organizer: 第24回日本癌免疫学会
• [Presentation] 大腸癌細胞の糖鎖発現解析と糖鎖改変の試み癌微小環境の制御機構の解析 (2021)
  • Author(s): Momoka Horiuchi, Yusuke Omi, Satoko Yamamoto, Hiroaki Kitazawa, Farhana Yesmin, Robiul Bhuiyan, Orie Tajima, Keiko Furukawa, Noboru Hashimoto, Koichi Furukawa
  • Organizer: 日本癌学会学術総会
• [Presentation] Changes of integrins in glycolipid-modified melanoma-derived exosomes (2021)
  • Author(s): Kei Kaneko, Yuhsuke Ohmi, Mariko Kambe, Qi Li, Yesmin Farhana, Satoko Yamamoto, Yoko Kitaura, Takako Ito, Robiul H. Bhuiyan, Orie Tajima, Koichi Furukawa, Keiko Furukawa
  • Organizer: 日本癌学会学術総会
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2021)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Momoka Horiuchi, Tetsuya Okajima, Satoko Yamamoto, Keiko Furukawa, Koichi Furukawa
  • Organizer: 日本癌学会学術総会
• [Presentation] Unique effects of expression of asialo-series ganglioside GD1alpha in human cancer cell lines (2021)
  • Author(s): Bhuiyan Robiul H., Farhana Yesmin, Yuji Kondo, Yuki Ohkawa, Yuhsuke Ohmi, Pu Zhang, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Organizer: 日本癌学会学術総会
• [Presentation] 完全マウス化による新たなCAR-T療法評価系の構築 (2021)
  • Author(s): 根岸航平、藤原 弘、赤堀 泰、三輪啓志、王 立楠、古川鋼一、珠玖 洋
  • Organizer: 日本癌学会学術総会
• [Presentation] Ganglioside GD2 cooperate with integrin in the enhancement of malignant properties of melanoma cells (2021)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Momoka Horiuchi, Tetsuya Okajima, Satoko Yamamoto, Kei Kaneko, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第94回日本生化学会大会
• [Presentation] スフィンゴ糖脂質による肝脂肪蓄積制御メカニズムの検討 (2021)
  • Author(s): 田島織絵、古川圭子、古川鋼一
  • Organizer: 第94回日本生化学会大会
• [Presentation] ヒトグリオーマ細胞由来エクソソームに発現するガングリオシドの機能解析 (2021)
  • Author(s): 大海雄介、Li Qi、山本聡子、北浦洋子、伊藤多佳子、佐藤詩歩、金子 慶、神戸眞理子、イエスミン ファーハナ、田島織絵、モハンマド A ハスナット、河原敏男、古川圭子、古川鋼一
  • Organizer: 第94回日本生化学会大会
• [Presentation] 初代培養アストロサイト上に発現するガングリオシドの役割 (2021)
  • Author(s): 佐藤詩歩、北浦洋子、伊藤多佳子、田島織絵、古川圭子、河原敏男、古川鋼一、大海雄介
  • Organizer: 第94回日本生化学会大会
• [Presentation] Ganglioside GD3 enhances the expression of integrins on extracellular vesicles (EVs) to exert its functions (2021)
  • Author(s): Kei Kaneko, Ohmi Yuhsuke, Kambe Mariko, Farhana Yesmin, Yamamoto Satoko, Kitaura Yoko, Ito Takako, Tajima Orie, Furukawa Koichi, Furukawa Keiko
  • Organizer: 第40回日本糖質学会年会
• [Presentation] アストロサイトに発現するガングリオシドの機能解析と近傍分子の同定 (2021)
  • Author(s): 佐藤詩歩、北浦洋子、伊藤多佳子、田島織絵、小谷典弘、本家孝一、古川圭子、河原敏男、古川鋼一、大海雄介
  • Organizer: 第40回日本糖質学会年会
• [Presentation] がん由来エクソソーム上に発現するガングリオシドのがん微小環境への影響 (2021)
  • Author(s): 大海雄介、Li Qi、山本聡子、北浦洋子、伊藤多佳子、佐藤詩歩、金子 慶、神戸眞理子、イエスミン ファーハナ、田島織絵、モハンマド A ハスナット、古川圭子、古川鋼一
  • Organizer: 第40回日本糖質学会年会
• [Presentation] ヒト大腸癌細胞による糖脂質発現と機能の解析 (2021)
  • Author(s): 堀内萌々花、橋本登、大海雄介、山本聡子、北沢裕昭、Yesmin Farhana、田島織絵、古川圭子、古川鋼一
  • Organizer: 第40回日本糖質学会年会
• [Presentation] Elucidation of unique function of ganglioside GD2 and its action mechanisms in human melanoma (2021)
  • Author(s): Yesmin F, Robiul H. Bhuiyan、Yuhsuke Ohmi、 Momoka Horiuchi、Tetsuya Okajima、Satoko Yamamoto、Kei Kaneko、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第40回日本糖質学会年会
• [Presentation] Different expression of integrins in ganglioside-remodeling melanoma EVs (2021)
  • Author(s): Kei Kaneko, Yuhsuke Ohmi, Mariko Kambe, Yesmin Farhana, Momoka Horiuchi, Yoko Kitaura, Takako Ito, Orie Tajima, Koichi Furukawa and Keiko Furukawa
  • Organizer: 国際EV学会（ISEV)
  • Int'l Joint Research
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2021)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Momoka Horiuchi, Tetsuya Okajima , Kei Kaneko, Keiko Furukawa, Koichi Furukawa
  • Organizer: 国際EV学会（ISEV）
  • Int'l Joint Research
• [Presentation] GD3/GD2-expressing glioma-derived EVs enhance malignant properties of gliomas, and regulate tumor microenvironments (2021)
  • Author(s): Koichi Furukawa, Yuhsuke Ohmi, Qi Li, Hiroaki Kitasawa, Yesmin Farhana, Satoko Yamamoto, Yoko Kitaura, Takako Ito, Kei Kaneko, Mariko Kambe, Keiko Furukawa
  • Organizer: 国際EV学会（ISEV）
  • Int'l Joint Research
• [Presentation] ヒトグリオーマ細胞由来エクソソームの機能解析 (2021)
  • Author(s): 大海雄介、Li Qi、山本聡子、北浦洋子、伊藤多佳子、金子 慶、神戸眞理子、イエスミン ファーハナ、田島織絵、モハンマド A ハスナット、古川圭子、古川鋼一
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] 糖脂質リモデリング腫瘍細胞由来細胞外小胞の機能解析 (2021)
  • Author(s): LIU QINYUE・澤田晋一・佐々木善浩・古川鋼一・秋吉一成
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] ガングリオシド改変メラノーマ細胞由来EVsにおけるintegrin の発現パターンとがん形質機能の解析 (2021)
  • Author(s): 金子 慶、大海雄介、神戸真理子、ファーハナ イエスミン、山本聡子、北浦洋子、伊藤多佳子、田島織絵、古川鋼一、古川圭子
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2021)
  • Author(s): Farhana Yesmin、Robiul H. Bhuiyan、Yuhsuke Ohmi、Momoka Horiuchi、Tetsuya Okajima、Satoko Yamamoto、Kei Kaneko、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] がんの進行阻害作用を持つCTLエクソソームと その作用を持たない CTL EV の生理学的性質 の検討 (2021)
  • Author(s): 瀬尾尚宏、中村純子、金田次弘、舘野浩章、下田麻子、一木隆範、古川鋼一、平林淳、秋吉一成、珠玖洋
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] Critical role of IRE1α in cholera toxin-induced interleukin-1β production from resident peritoneal macrophages (2021)
  • Author(s): Izumi Sasaki, Yuri Fukuda-Ohta, Naoko Wakaki-Nishiyama, Chihiro Nakai, Shuhei Morita, Daisuke Okuzaki, Mika Kaku, Kanta Sawase, Asumi Yamamoto, Takashi Kato, Takashi Orimo, Koichi Furukawa, Tsuneyasu Kaisho
  • Organizer: 第16回国際エンドトキシン・自然免疫学会
• [Presentation] An endoplasmic reticulum stress sensor IRE1alpha is involved in cholera toxin-induced interleukin-1beta production from tissue resident macrophages (2021)
  • Author(s): Izumi Sasaki, Yuri Fukuda-Ohta, Naoko Wakaki-Nishiyama, Chihiro Nakai, Shuhei Morita, Daisuke Okuzaki, Mika Kaku, Kanta Sawase, Takashi Kato, Takashi Orimo, Koichi Furukawa, Tsuneyasu Kaisho
  • Organizer: 第28回日本免疫毒性学会学術年会
• [Presentation] コレラ毒素による免疫アジュバント活性における小胞体ストレスセンサーIRE1alphaの機能的意義 (2021)
  • Author(s): 佐々木泉、中井千尋、大田有里、西山奈央子、森田修平、奥崎大介、三並桃佳、林茉里、張江伊水、田中日向子、加藤喬、折茂貴是、古川鋼一、改正恒康
  • Organizer: 第25回日本がん免疫学会総会
• [Presentation] Extracellular vesicles secreted from melanoma cell lines contain high levels of integrins and similar profiles of ganglioside species with parent cells (2020)
  • Author(s): Koichi Furukawa, Yuya Senoo, Mariko Kambe, Kazutaka Ikeda, Yuhsuke Ohmi, Satoko Yamamoto, Iori Kobayashi, Yuki Ohkawa, Keiko Furukawa
  • Organizer: ISEV (国際細胞外小胞学会)
  • Int'l Joint Research
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2020)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Tetsuya Okajima, Satoko Yamamoto, Keiko Furukawa, Koichi Furukawa
  • Organizer: 日本癌学会総会
• [Presentation] Roles of asialo-series ganglioside GD1alpha in human cancer cell lines (2020)
  • Author(s): Robiul Hasan Bhuiyan, Farhana Yesmin, Yuji Kondo, Yuki Ohkawa, Yuhsuke Ohmi, Pu Zhang, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Organizer: 日本癌学会総会
• [Presentation] スフィンゴ糖脂質による肝インスリンシグナル制御を介した脂肪肝抑制機構の検討 (2020)
  • Author(s): 田島織絵、古川圭子、古川鋼一
  • Organizer: 第93回日本生化学会
• [Presentation] 初代培養アストロサイト上に発現するガングリオシドの機能解析 (2020)
  • Author(s): 佐藤詩歩、北浦洋子、伊藤多佳子、古川圭子、河原敏男、古川鋼一、大海雄介
  • Organizer: 第29回日本糖質学会年会
• [Presentation] The roles of unfold protein responses in cholera toxin B-induced interleukine-1beta production (2020)
  • Author(s): Izumi Sasaki, Takashi Orimo, Isui Harie, Yuko Takisawa, Koichi Furukawa, Tsuneyasu Kaisho
  • Organizer: 第27回日本免疫毒性学会学術年会
• [Presentation] The role of IRE1α in cholera toxin B-induced interleukine-1β production from resident peritoneal macrophages (2020)
  • Author(s): Izumi Sasaki, Chihiro Nakai, Shuhei Morita, Daisuke Okuzaki, Yuri Fukuda-Ohta, Takashi Orimo, Hiroaki Hemmi, Koichi Furukawa, Tsuneyasu Kaisho
  • Organizer: 第27回マクロファージ分子細胞生物学国際シンポジウム
  • Int'l Joint Research
• [Presentation] Novel cellular immunotherapy using GD2-specific CAR gene-modified allogeneic gamma/delta-T cells against solid cancers (2020)
  • Author(s): Hiroshi Fujiwara, Yasushi Akahori, Hiroshi Miwa, Linan Wang, Chisaki Hyuga, Yusuke Ohmi, Yoshimasa Tanaka, Keiko Furukawa, Koichi Furukawa, Yoshihiro Miyahara, Hiroshi Shiku
  • Organizer: 第82回日本血液学会学術集会
• [Presentation] Ig上のシアル酸機能の解析とSALSA法によるマウス脳組織のシアル化糖鎖構造解析 (2018)
  • Author(s): 大海 雄介, 古川 鋼一
  • Organizer: 第37回 日本糖質学会年会
  • Invited
• [Presentation] 糖鎖改変に伴う肝脂肪蓄積抑制の分子メカニズム (2018)
  • Author(s): 田島 織絵, 古川 圭子, 古川 鋼一
  • Organizer: 第91回日本生化学会大会
• [Presentation] 癌関連スフィンゴ糖脂質による細胞動態の分別的制御に関するタイムラプス解析 (2018)
  • Author(s): 古川 圭子, 神戸 真理子, 大海 雄介, 大川 祐樹, 竹内 理香, 田島 織絵, 安藤 弘宗, 古川 鋼一
  • Organizer: 第91回日本生化学会大会
• [Presentation] 癌転移におけるエクソソームの役割の解析 (2018)
  • Author(s): 児玉 佳輝, 章 青, 大海 雄介, Hasan Bhuiyan Robiul, Yesmin Farhana, 山本 聡子, 古川 圭子, 古川 鋼一
  • Organizer: 第91回日本生化学会大会
• [Presentation] グリオーマーの微小環境調節におけるガングリオシドGD3の役割 (2018)
  • Author(s): 張 璞, 大川 祐樹, 大海 雄介, Robiul H.Bhuiyan, 加藤 彰, 古川 圭子, 岡島 徹也, 若林 俊彦, 古川 鋼一
  • Organizer: 第77回 日本癌学会学術総会
• [Presentation] 癌関連ガングリオシド合成酵素遺伝子の発現制御機構シグナルの解析：シグナル伝達阻害剤による検討 (2018)
  • Author(s): 竹内 理香, 宮田麻衣子, ウプル ジャヤデワン, 田島 織絵, 神戸 真理子, 古川 鋼一, 古川 圭子
  • Organizer: 第77回 日本癌学会学術総会
• [Presentation] 糖鎖リモデリング癌細胞由来エキソソームの膜分子組成と分泌機構の解析 (2018)
  • Author(s): 小林 伊織, 大海 雄介, 古川 圭子, ブイヤン ロビウル, 張 璞, 古川 鋼一
  • Organizer: 第77回 日本癌学会学術総会
• [Presentation] Roles of asialo-series ganglioside GD1alpha in human cancer cell lines (2018)
  • Author(s): Robiul H.Bhuiyan, Yuji Kondo, Yuki Ohkawa, Yuhsuke Ohmi, Pu Zhand, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第77回 日本癌学会学術総会
• [Remarks] 中部大学生命健康科学部糖鎖生物学講座
  • URL: http://koichichubu.starfree.jp
• [Remarks] 癌関連糖鎖を標的にした新規癌治療法の開発
  • URL: https://www.chubu.ac.jp/about/faculty/profile/835a9495737bccf6f4128d87c4aabc80b41827bc.pdf