Establishment of Identification of TCR antigen and prediction of side effects for TCR gene therapy
Project/Area Number |
18H02689
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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Research Institution | University of Toyama |
Principal Investigator |
Tatsuihko Ozawa 富山大学, 学術研究部医学系, 准教授 (10432105)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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Keywords | T細胞受容体 / TCR遺伝子療法 / がん免疫療法 / ペプチド/MHC / TCR / レパートリー解析 / 抗原同定 |
Outline of Final Research Achievements |
Adoptive T cell therapy using TCR-modified T cells is a promising next-generation immunotherapy. However, it was not easy to isolate a functional TCR, and to analyze the reactivity and predict the side effects. In this study, we have established a method to obtain TCRs that respond to the original cancer cells from tumor-infiltrating lymphocytes, and technically simple method to generate functional p/MHC that analyze the reactivity of TCRs. These results may contribute to development of adoptive T cell therapy using TCR-modified T cells.
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Academic Significance and Societal Importance of the Research Achievements |
がんに対する新しい治療法として、T細胞が持っているT細胞受容体(TCR)を使った免疫療法、TCR遺伝子療法の研究が進んでいる。しかしながらTCR遺伝子療法に用いたTCRが類似した配列を持つ自己抗原を認識して重篤な副作用を起こす症例が報告されている。本研究では、TCRの反応性を解析する基盤作りを行い、TCRの反応性を解析する簡便なツールであるペプチド/MHCの容易な作製法を確立した。本研究成果を応用することでTCR遺伝子療法のさらなる発展が期待される。
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein.2021
Author(s)
Tani H, Kimura M, Tan L, Yoshida Y, Ozawa T, Kishi H, Fukushi S, Saijo M, Sano K, Suzuki T, Kawasuji H, Ueno A, Miyajima Y, Fukui Y, Sakamaki I, Yamamoto Y, Morinaga Y
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Journal Title
Virology Journal
Volume: 18
Issue: 1
Pages: 16-16
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein.2021
Author(s)
Kimura M, Egawa K, Ozawa T, Kishi H, Shimojima M, Taniguchi S, Fukushi S, Fujii H, Yamada H, Tan L, Sano K, Katano H, Suzuki T, Morikawa S, Saijo M, Tani H.
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Journal Title
Virology
Volume: 556
Pages: 124-132
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Relationship between T cell receptor clonotype and PD-1 expression of tumor-infiltrating lymphocytes in colorectal cancer.2020
Author(s)
Sukegawa K, Shitaoka K, Hamana H, Kobayashi E, Miyahara Y, Fujii K, Tsuda K, Saeki S, Nagata T, Ozawa T, Saito S, Fujii T, Muraguchi A, Shiku H, Kishi H.
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Journal Title
Eur. J. Immunol.
Volume: 50
Issue: 10
Pages: 1580-1590
DOI
Related Report
Peer Reviewed
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[Journal Article] Physiologic Target, Molecular Evolution, and Pathogenic Functions of a Monoclonal Anti-Citrullinated Protein Antibody Obtained From a Patient With Rheumatoid Arthritis.2020
Author(s)
Ozawa T, Ouhara K, Tsuda R, Munenaga S, Kurihara H, Kohno H, Hamana H, Kobayashi E, Taki H, Tobe K, Sugiyama E, Muraguchi A, Kishi H.
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Journal Title
Arthritis Rheumatol.
Volume: 72
Issue: 12
Pages: 2040-2049
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A frequent nonsense mutation in exon 1 across certain HLA-A and -B alleles in leukocytes of patients with acquired aplastic anemia.2020
Author(s)
Mizumaki H, Hosomichi K, Hosokawa K, Yoroidaka T, Imi T, Zaimoku Y, Katagiri T, Nguyen MAT, Tran DC, Elbadry MIY, Chonabayashi K, Yoshida Y, Takamatsu H, Ozawa T, Azuma F, Kishi H, Fujii Y, Ogawa S, Tajima A, Nakao S.
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Journal Title
Haematologica
Volume: -
Issue: 6
Pages: 247809-247809
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses.2020
Author(s)
Miyoshi S, Tokunaga S, Ozawa T, Takeda H, Aono M, Miyoshi T, Kishi H, Muraguchi A, Shimizu S, Nozawa A, Sawasaki T.
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Journal Title
PloS One
Volume: 15
Issue: 4
Pages: e0229196-e0229196
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Autoantibodies reactive to PEP08 are clinically related with morbidity and severity of interstitial lung disease in connective tissue diseases.2018
Author(s)
Okumura M, Ozawa T, Hamana H, Norimatsu Y, Tsuda R, Kobayashi E, Shinoda K, Taki H, Tobe K, Imura J, Sugiyama E, Kishi H, Muraguchi A.
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Journal Title
Eur J Immunol.
Volume: 48
Issue: 10
Pages: 1717-1727
DOI
Related Report
Peer Reviewed
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[Presentation] The identification of a common loss-function mutation across different HLA class alleles frequently detected in patients with acquired aplastic anemia.2019
Author(s)
Mizumaki H, Hosomichi K, Tanabe M, Yoroidaka T, Imi T, Hosokawa K, Katagiri T, Takamatsu H, Ozawa T, Azuma F, Kishi H, Tajima A, Nakao S.
Organizer
24th Congress of European Hematology Association
Related Report
Int'l Joint Research
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[Presentation] Loss-of function mutations in HLA-classⅠalleles in acquire aplastic anemia: evidence for the involvement of limited classⅠalleles in the auto-antigen presentation of aplastic anemia2018
Author(s)
Mizumaki H, Hosomichi K, Mikoto T, Yoroidaka T, Imi T, Zaimoku Y, Hosokawa K, Katagiri T, Takamatsu H, Ozawa T, Azuma F, Kishi H, Tajima A, Nakano S
Organizer
60th ASH Annual Meeting and Exposition
Related Report
Int'l Joint Research
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[Presentation] 子宮体癌患者における末梢血CD8T細胞のTCRレパートリー解析2018
Author(s)
津田桂, 岸裕幸, 中島彰俊, 浜名洋, 牛島明美, 津田さやか, 島友子, 下岡清美, 小林栄治, 小澤龍彦, 吉野修, 齋藤滋
Organizer
第33回日本生殖免疫学会総会
Related Report
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