| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
Optogenetic techniques can be used to manipulate cellular activity with high spatiotemporal resolution. A widely used optogenetic tool is channelrhodopsin-2, which can be used to control neuronal activity at the cellular level. However, no optogenetic tool has been able to induce synaptic plasticity at the level of a single synapse. We have therefore developed a novel tool that enables optical manipulation of synapses. Specifically, we developed a light-activatable CaMKII by fusing CaMKIIα with the light-sensitive domain LOV2. This enabled us to activate light-activatable CaMKII in a single spine and induce long-term potentiation (LTP) in hippocampal neurons.
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