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Construction of A New Paradigm for Diagnosis and Treatment of Atherosclerosis Obliterans

Research Project

Project/Area Number 18H02734
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 52010:General internal medicine-related
Research InstitutionKagoshima University

Principal Investigator

Yamakuchi Munekazu  鹿児島大学, 医歯学域医学系, 准教授 (20325814)

Co-Investigator(Kenkyū-buntansha) 丸山 征郎  鹿児島大学, 医歯学総合研究科, 特任教授 (20082282)
大山 陽子  鹿児島大学, 鹿児島大学病院, 医員 (20583470)
竹之内 和則  鹿児島大学, 医歯学域医学系, 助教 (30646758)
橋口 照人  鹿児島大学, 医歯学域医学系, 教授 (70250917)
原田 陽一郎  地方独立行政法人大阪府立病院機構大阪国際がんセンター(研究所), その他部局等, 糖鎖オンコロジー部主任研究員 (80464147)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords動脈硬化 / microRNA / 血管内皮機能 / ASO / miRNA / exosome / 血管内皮細胞
Outline of Final Research Achievements

Atherosclerosis obliterans (ASO) develops in patients with underlying diseases such as diabetes, and early diagnosis and treatment for ASO are difficult because of the lack of clear subjective symptoms and the lack of evaluation methods other than physiological tests. The purpose of this study was to examine whether microRNAs contained in extracellular vesicles in blood can be applied to the diagnosis and treatment of ASO, and the following results were obtained.
1. We identified microRNAs in serum that are characteristic of ASO patients. 2. We found the relationship between serum microRNAs and vascular endothelial molecules in patients with arterial fibrillation who underwent catheter ablation. 3. We reported the relationship between serum microRNAs and vascular endothelial growth factor in patients undergoing coronary artery bypass surgery. 4. We showed platelet dynamics in patients with aortic valve stenosis.

Academic Significance and Societal Importance of the Research Achievements

閉塞性動脈硬化症(ASO)は、60歳以上の男性、喫煙者に多いことが知られており、また冠動脈疾患や脳血管疾患など重大な全身血管障害が隠れている危険性がある。本研究で明らかにしたマイクロRNAの更なる研究により、ASOの病態を正確に把握し早期診断が可能となれば、罹患患者数・生活習慣病患者数の減少が見込まれ、医療経済面のメリット、社会的意義がある。また、ASOの複雑な病態を統合的に再構成するツールとしてマイクロRNAが使用できる可能性があり、マイクロRNAの血管内皮機能検査への応用・血管病の治療の分子メカニズムの解明につながり、学術的意義を有する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (21 results)

All 2021 2020 2019 2018

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Dynamic changes in platelets caused by shear stress in aortic valve stenosis2021

    • Author(s)
      Hideaki Kanda, Munekazu Yamakuchi, Kazuhisa Matsumoto, Kosuke Mukaihara, Yoshiya Shigehisa, Shuji Tachioka, Masashi Okawa, Kazunori Takenouchi, Yoko Oyama, Teruto Hashiguchi, and Yutaka Imotoa
    • Journal Title

      Clinical Hemorheology and Microcirculation

      Volume: 77 Issue: 1 Pages: 71-81

    • DOI

      10.3233/ch-200928

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bivalent property of intra-platelet VWF in liver regeneration and HCC recurrence: A prospective multicenter study2019

    • Author(s)
      Aryal Bibek、Yamakuchi Munekazu、Shimizu Toshiaki、Kadono Jun、Furoi Akira、Gejima Kentaro、Takenouchi Kazunori、Komokata Teruo、Hashiguchi Teruto、Imoto Yutaka
    • Journal Title

      Cancer Biomarkers

      Volume: 26 Issue: 1 Pages: 51-61

    • DOI

      10.3233/cbm-190168

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intra-platelet Serotonin as a Biomarker in HCC Recurrence: When Time Matters2019

    • Author(s)
      Aryal Bibek、Yamakuchi Munekazu、Hashiguchi Teruto、Imoto Yutaka
    • Journal Title

      Journal of Cancer

      Volume: 10 Issue: 11 Pages: 2384-2385

    • DOI

      10.7150/jca.30696

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of high-mannose-type glycan-specific lectin from Oscillatoria Agardhii for affinity isolation of tumor-derived extracellular vesicles2019

    • Author(s)
      Yamamoto Mika、Harada Yoichiro、Suzuki Takehiro、Fukushige Tomoko、Yamakuchi Munekazu、Kanekura Takuro、Dohmae Naoshi、Hori Kanji、Maruyama Ikuro
    • Journal Title

      Analytical Biochemistry

      Volume: 580 Pages: 21-29

    • DOI

      10.1016/j.ab.2019.06.001

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells2019

    • Author(s)
      Koriyama Toyoyasu、Yamakuchi Munekazu、Takenouchi Kazunori、Oyama Yoko、Takenaka Hiroyoshi、Nagakura Takumi、Masamoto Izumi、Hashiguchi Teruto
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 508 Issue: 2 Pages: 608-613

    • DOI

      10.1016/j.bbrc.2018.11.093

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Predictive Value of Diminished Serum PDGF-BB after Curative Resection of Hepatocellular Cancer2019

    • Author(s)
      Aryal Bibek、Yamakuchi Munekazu、Shimizu Toshiaki、Kadono Jun、Furoi Akira、Gejima Kentaro、Komokata Teruo、Koriyama Chihaya、Hashiguchi Teruto、Imoto Yutaka
    • Journal Title

      Journal of Oncology

      Volume: 2019 Pages: 1-8

    • DOI

      10.1155/2019/1925315

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endothelial Cell Aging: How miRNAs Contribute?2018

    • Author(s)
      Yamakuchi Munekazu、Hashiguchi Teruto
    • Journal Title

      Journal of Clinical Medicine

      Volume: 7 Issue: 7 Pages: 170-170

    • DOI

      10.3390/jcm7070170

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deciphering Platelet Kinetics in Diagnostic and Prognostic Evaluation of Hepatocellular Carcinoma2018

    • Author(s)
      Aryal Bibek、Yamakuchi Munekazu、Shimizu Toshiaki、Kadono Jun、Furoi Akira、Gejima Kentaro、Komokata Teruo、Hashiguchi Teruto、Imoto Yutaka
    • Journal Title

      Canadian Journal of Gastroenterology and Hepatology

      Volume: 2018 Pages: 1-9

    • DOI

      10.1155/2018/9142672

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Therapeutic implication of platelets in liver regeneration ?hopes and hues2018

    • Author(s)
      Aryal Bibek、Yamakuchi Munekazu、Shimizu Toshiaki、Kadono Jun、Furoi Akira、Gejima Kentaro、Komokata Teruo、Hashiguchi Teruto、Imoto Yutaka
    • Journal Title

      Expert Review of Gastroenterology & Hepatology

      Volume: 12 Issue: 12 Pages: 1219-1228

    • DOI

      10.1080/17474124.2018.1533813

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 冠動脈バイパス術周術期におけるmicroRNA-126についての検討2020

    • Author(s)
      山口 宗一, 向原 公介, 上田 英昭, 重久 善哉, 大川 政士, 松本 和久, 竹之内 和則, 大山 陽子, 東 貞行, 藤崎 知園子, 井本 浩, 橋口 照人
    • Organizer
      第67回日本臨床検査医学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] microRNAによる血管平滑筋石灰化制御の検討2020

    • Author(s)
      山口 宗一, 上田 英昭, 大川 政士, 向原 公介, 重久 善哉, 松本 和久, 竹之内 和則, 大山 陽子, 東 貞行, 藤崎 知園子, 井本 浩, 橋口 照人
    • Organizer
      第67回日本臨床検査医学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 冠動脈バイパス術周術期における血小板内VEGF-Aの変化についての検討2020

    • Author(s)
      山口 宗一, 向原 公介, 上田 英昭, 重久 善哉, 大川 政士, 松本 和久, 竹之内 和則, 大山 陽子, 井本 浩, 橋口 照人
    • Organizer
      第60回日本臨床化学会年次学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マイクロRNAが血管平滑筋の石灰化に与える影響の解析2020

    • Author(s)
      上田 英昭, 山口 宗一, 松本 和久, 大川 政士, 向原 公介, 重久 喜哉, 橋口 照人, 井本 浩
    • Organizer
      第120回日本外科学会定期学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 冠動脈バイパス術周術期における血小板内VEGFの推移2020

    • Author(s)
      向原 公介, 山口 宗一, 松本 和久, 上田 英昭, 重久 喜哉, 大川 政士, 橋口 照人, 井本 浩
    • Organizer
      第120回日本外科学会定期学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] レジオネラ菌感染におけるマクロファージ様細胞U937のmicroRNAを介した応答機構2019

    • Author(s)
      山口 宗一, 郡山 豊泰, 竹之内 和則, 大山 陽子, 政元 いずみ, 橋口 照人
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大動脈弁狭窄症に対する大動脈弁置換術前後での末梢血、凝固系の検討2019

    • Author(s)
      上田英昭、松本和久、山口宗一、竹之内和則、大山陽子、橋口照人、井本 浩
    • Organizer
      第51回日本臨床検査自動化学学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulation of von Willebrand factor release in endothelial cells2019

    • Author(s)
      山口 宗一, 竹之内 和則, 大山 陽子, 橋口 照人
    • Organizer
      第41回日本血栓止血学会学術集会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] miR-375 attenuated innate immune response in bronchial epithelial cell through modulate of JAK2-STAT1 pathway2019

    • Author(s)
      Koichi Takagi, Kentaro Machida, Takahiro Matsuyama, Kota Sakaue, Kiyotaka Kondo, Munekazu Yamakuchi, Teruto Hashiguchi, Hiromasa Inoue
    • Organizer
      American Thoracic Society International Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Book] 細胞 p53分子と血管炎症のかかわり2020

    • Author(s)
      山口宗一
    • Total Pages
      4
    • Publisher
      ニューサイエンス社
    • Related Report
      2019 Annual Research Report
  • [Book] 臨床病理 miRNAによる自然免疫制御機構2019

    • Author(s)
      山口 宗一, 郡山 豊泰, 清水 利昭, 竹之内 和則, 大山 陽子, 政元 いずみ, 橋口 照人
    • Total Pages
      12
    • Publisher
      日本臨床検査医学会
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗 VEGF-A 抗体による癌に対する治療応答性を予測又は決定するための方法2020

    • Inventor(s)
      橋口照人・山口宗一・竹之内和則・大川政士・井本浩以下2名
    • Industrial Property Rights Holder
      橋口照人・山口宗一・竹之内和則・大川政士・井本浩以下2名
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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