Study to establish the bioassay system for peripheral route infection of various prions
| Project/Area Number |
18H02738
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 52020:Neurology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | プリオン / 末梢ルート / FDC / 腹腔内投与 / 抹消ルート |
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| Outline of Final Research Achievements |
In Japanese cases with dura-grafted CJD, we identified the ratio of M1 prion and V2 prion similarly as the ratio of European sporadic CJD. However, the ratio of European cases with Growth hormone-associated CJD was quite different from the ratio of European sporadic CJD. Because the infection route is different between both infections, we compared the infection between intracranial and intraperitoneal administration. In intracranial administration, 100% of knock-in mice were infected with M1 or V2 prion. However, in intraperitoneal administration, M1 prion-infected mice were fewer than V2-infected mice. In particular, the knock-in mice with 129Met/Met were hardly infected with M1 prions. These results well explain the different ratio between dura grafted CJD and growth hormone associated CJD.
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| Academic Significance and Societal Importance of the Research Achievements |
プリオン感染は、医療関係者が恐怖に駆られる致死性の感染症である。現に、針刺し事故などで相談をうけることが多い。本研究成果ではわが国でも最も多いM1プリオンは、脳を用いた感染実験でもわが国で最も多い遺伝子型である129Met/Metのモデル動物を用いた抹消ルートからは感染しないことを明らかとした。これは、頭蓋内投与がほぼ１００％感染する結果との際立った違いであり、針刺し事故などの説明に役立つ根拠となる。
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Correction to: In Vitro Seeding Activity of Glycoform-Deficient Prions from Variably Protease-Sensitive Prionopathy and Familial CJD Associated with PrPV180I Mutation2019
Author(s)
Wang Z, Yuan J, Shen P, Abskharon R, Lang Y, Dang J, Adornato A, Xu L, Chen J, Feng J, Moudjou M, Kitamoto T, Lee HG, Kim YS, Langeveld J, Appleby B, Ma J, Kong Q, Petersen RB, Zou WQ, Cui L
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Journal Title
Mol Neurobiol
Volume: 56
Pages: 5470-5470
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] In Vitro Seeding Activity of Glycoform-Deficient Prions from Variably Protease-Sensitive Prionopathy and Familial CJD Associated with PrPV180I Mutation2019
Author(s)
Wang Z, Yuan J, Shen P, Abskharon R, Lang Y, Dang J, Adornato A, Xu L, Chen J, Feng J, Moudjou M, Kitamoto T, Langeveld J, Appleby B, Ma J, Kong Q, Petersen RB, Zou WQ, Cui L
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Journal Title
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Iatrogenic Creutzfeldt-Jakob disease with Amyloid-β pathology: an international study2018
Author(s)
Cali I, Cohen ML, Haik S, Parchi P, Giaccone G, Collins SJ, Kofskey D, Wang H, McLean CA, Brandel JP, Privat N, Sazdovitch V, Duyckaerts C, Kitamoto T, et al
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Journal Title
Acta Neuropathol Commun
Volume: 6
Pages: 5-5
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Creutzfeldt-Jakob disease with Alzheimer pathology, presenting with status epilepticus following repeated partial seizures: a case report and literature review.2018
Author(s)
Miyake K, Hara T, Oshima E, Kawada K, Ishizu H, Yamauchi Y, Satoh K, Kitamoto T, Takenoshita S, Terada S, Yamada N
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Journal Title
BMC Neurol
Volume: 18(1)
Pages: 54-54
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Neuroimaging-pathological correlations of [18F]THK5351 PET in progressive supranuclear palsy.2018
Author(s)
Ishiki A, Harada R, Kai H, Sato N, Totsune T, Tomita N, Watanuki S, Hiraoka K, Ishikawa Y, Funaki Y, Iwata R, Furumoto S, Tashiro M, Sasano H, Kitamoto T, Kudo Y, Yanai K, Furukawa K, Okamura N, Arai H.
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Journal Title
Acta Neuropathol Commun.
Volume: 6
Pages: 53-53
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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