Novel molecular basis underlying lethal arrhythmic syndrome due to mutations in cardiac Na/Ca exchanger gene

• Project/Area Number: 18H02808
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: MAKITA Naomasa 国立研究開発法人国立循環器病研究センター, 研究所, 副所長 (00312356)
• Co-Investigator(Kenkyū-buntansha): 牧山 武 京都大学, 医学研究科, 特定講師 (30528302) ; 渡邊 泰秀 浜松医科大学, 医学部, 教授 (50305380)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 早期再分極症候群 / Na/Ca交換体 / 遺伝子変異 / 突然死 / ゲノム編集 / パッチクランプ / 致死性不整脈 / Na-Ca交換体 / QT短縮症候群 / ノックインマウス / コンピュータシミュレーション / J波症候群 / 心臓突然死

Outline of Final Research Achievements

Comprehensive genetic screening of patients with early repolarization syndrome (ERS) identified six novel mutations in the cardiac Na/Ca exchanger(NCX1) gene. Whole-genome sequencing confirmed cosegregation of NCX1 mutations within two families. Mutant NCX1 cDNAs transiently transfected in HEK293 cells showed reduced 45Ca uptake and NCX1 current amplitudes associated with membrane trafficking defect. Programmed electrical stimulation of burst pacing mode at the right ventricular apex reproducibly elicited ventricular tachycardia in NCX1-L215H knock-in mice that were established by CRISPR/Cas9 genome editing, but not in wild type mice. These data demonstrated that the cardiac NCX1 is a novel gene responsible for ERS, which is characterized by J wave and predisposition to lethal ventricular arrhythmia.

Academic Significance and Societal Importance of the Research Achievements

心電図のJ波は長い間正常亜型と考えられてきた。最近J波を特徴とする特発性心室細動は、早期再分極症候群(ERS)という独立した致死性不整脈と認識されているが、未発症のERS患者とJ波陽性の健常人を心電図だけで鑑別することは困難である。本研究はこれまで未解明だったERSの新たな原因として、心筋Na/Ca 交換体(NCX1)の遺伝子異常を明らかにした。今後NCX1の変異と詳細な機能異常がさらに詳細に解明されれば、遺伝子解析結果に基づく心臓突然死のリスクを評価と層別化が可能になり、突然死の個別化予防医療の実現につながることが期待される。

Research Products

• [Journal Article] Impact of the T-wave characteristics on distinguishing arrhythmogenic right ventricular cardiomyopathy from healthy children (2021)
  • Author(s): Imamura Tomohiko、Sumitomo Naokata、Muraji Shota、Yasuda Kazushi、Nishihara Eiki、Iwamoto Mari、Tateno Shigeru、Doi Shozaburo、Hata Tadayoshi、Kogaki Shigetoyo、Horigome Hitoshi、Ohno Seiko、Ichida Fukiko、Nagashima Masami、Makiyama Takeru、Yoshinaga Masao
  • Journal Title: International Journal of Cardiology Volume: 323 Pages: 168-174
  • DOI: 10.1016/j.ijcard.2020.08.088
  • Peer Reviewed / Open Access
• [Journal Article] Enhancing rare variant interpretation in inherited arrhythmias through quantitative analysis of consortium disease cohorts and population controls (2020)
  • Author(s): 2.Walsh R, Lahrouchi N, Tadros R, Kyndt F, Glinge C, Postema PG, et al.
  • Journal Title: Genetics in Medicine Volume: 23 Issue: 1 Pages: 47-58
  • DOI: 10.1038/s41436-020-00946-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SCN5A Mutation Type and a Genetic Risk Score Associate Variably With Brugada Syndrome Phenotype in SCN5A Families (2020)
  • Author(s): 3.Wijeyeratne YD, Tanck MW, Mizusawa Y, Batchvarov V, Barc J, Crotti L, et al.
  • Journal Title: Circulation: Genomic and Precision Medicine Volume: 13 Issue: 6
  • DOI: 10.1161/circgen.120.002911
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cardiac Emerinopathy (2020)
  • Author(s): Ishikawa Taisuke、Ichida Fukiko、Makita Naomasa et al
  • Journal Title: Circulation: Arrhythmia and Electrophysiology Volume: 13 Issue: 10 Pages: 1165-1174
  • DOI: 10.1161/circep.120.008712
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transethnic Genome-Wide Association Study Provides Insights in the Genetic Architecture and Heritability of Long QT Syndrome (2020)
  • Author(s): Lahrouchi N, Tadros R, Crotti L, Makiyama T et al.
  • Journal Title: Circulation Volume: 142 Issue: 4 Pages: 324-338
  • DOI: 10.1161/circulationaha.120.045956
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Propranolol Attenuates Late Sodium Current in a Long QT Syndrome Type 3-Human Induced Pluripotent Stem Cell Model (2020)
  • Author(s): Hirose Sayako、Makiyama Takeru、Melgari Dario、Yamamoto Yuta、Wuriyanghai Yimin、Yokoi Fumika、Nishiuchi Suguru、Harita Takeshi、Hayano Mamoru、Kohjitani Hirohiko、Gao Jingshan、Kashiwa Asami、Nishikawa Misato、Wu Jie、Yoshimoto Jun、Chonabayashi Kazuhisa、Ohno Seiko、Yoshida Yoshinori、Horie Minoru、Kimura Takeshi
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 8 Pages: 761-761
  • DOI: 10.3389/fcell.2020.00761
  • Peer Reviewed / Open Access
• [Journal Article] Telethonin variants found in Brugada syndrome, J-wave pattern ECG, and ARVC reduce peak Nav 1.5 currents in HEK-293 cells (2020)
  • Author(s): Turker I, Makiyama T, Ueyama T, Shimizu A, Yamakawa M, Chen PS, Vatta M, Horie M, Ai T
  • Journal Title: Pacing Clin Electrophysiol Volume: 43 Issue: 8 Pages: 838-846
  • DOI: 10.1111/pace.13996
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Erratum to “Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients” (2020)
  • Author(s): Takaki T, Inagaki A, Chonabayashi K, Inoue K, Miki K, Ohno S, Makiyama T, Horie M, Yoshida Y.
  • Journal Title: Stem Cells International Volume: 2020 Pages: 1-2
  • DOI: 10.1155/2020/8765895
  • Peer Reviewed / Open Access
• [Journal Article] TBX5 R264K acts as a modifier to develop dilated cardiomyopathy in mice independently of T-box pathway (2020)
  • Author(s): Miyao N., Hata Y., Izumi H., Nagaoka R., Oku Y., Takasaki I., Ishikawa T., Takarada S., Okabe M., Nakaoka H., Ibuki K., Ozawa S., Yoshida T., Hasegawa H., Makita N., Nishida N., Mori H., Ichida F., Hirono K.
  • Journal Title: PLOS ONE Volume: 15 Issue: 4 Pages: 0227393-0227393
  • DOI: 10.1371/journal.pone.0227393
  • Peer Reviewed / Open Access
• [Journal Article] Desmin‐related myopathy characterized by non‐compaction cardiomyopathy, cardiac conduction defect, and coronary artery dissection (2020)
  • Author(s): Tamiya R., Saito Y., Fukamachi D., Nagashima K., Aizawa Y., Ohkubo K., Hatta T., Sezai A., Tanaka M, Ishikawa T., Makita N., Sumitomo N., Okumura Y.
  • Journal Title: ESC Heart Failure Volume: - Issue: 3 Pages: 1338-1343
  • DOI: 10.1002/ehf2.12667
  • Peer Reviewed / Open Access
• [Journal Article] Identification of transmembrane protein 168 mutation in familial Brugada syndrome. (2020)
  • Author(s): Shimizu A, Zankov DP, Sato A, Komeno M, Toyoda F, Yamazaki S, Makita T, Noda T, Ikawa M, Asano Y, Miyashita Y, Takashima S, Morita H, Ishikawa T, Makita N, Hitosugi M, Matsuura H, Ohno S, Horie M, Ogita H.
  • Journal Title: FASEB Journal Volume: 34 Issue: 5 Pages: 6399-6417
  • DOI: 10.1096/fj.201902991r
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 遺伝性不整脈のPrecision medicine (2020)
  • Author(s): 石川泰輔, 蒔田直昌
  • Journal Title: 週間 医学のあゆみ Volume: - Pages: 1279-1283
• [Journal Article] Brugada症候群の遺伝的背景 (2020)
  • Author(s): 石川泰輔, 蒔田直昌
  • Journal Title: 別冊・医学のあゆみ 遺伝性心血管疾患のすべて Volume: - Pages: 87-90
• [Journal Article] Calmodulin mutations and life-threatening cardiac arrhythmias: insights from the International Calmodulinopathy Registry (2019)
  • Author(s): Crotti Lia、Spazzolini Carla、Tester David J、Ohno Seiko、Ackerman Michael J、Schwartz Peter J, et al.
  • Journal Title: European Heart Journal Volume: 40 Issue: 35 Pages: 2964-2975
  • DOI: 10.1093/eurheartj/ehz311
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 遺伝性不整脈の遺伝子診断：有効性と限界 (2019)
  • Author(s): 石川泰輔, 蒔田直昌
  • Journal Title: 循環器病研究の進歩 Recent Advances in Cadiovascular Disease Volume: 40 Pages: 58-66
• [Journal Article] Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients (2019)
  • Author(s): Takaki Tadashi、Inagaki Azusa、Chonabayashi Kazuhisa、Inoue Keiji、Miki Kenji、Ohno Seiko、Makiyama Takeru、Horie Minoru、Yoshida Yoshinori
  • Journal Title: Stem Cells International Volume: 2019 Pages: 1-12
  • DOI: 10.1155/2019/7532657
  • NAID: 120006634620
  • Peer Reviewed / Open Access
• [Journal Article] Mutant KCNJ3 and KCNJ5 potassium channels as novel molecular targets in bradyarrhythmias and atrial fibrillation. (2019)
  • Author(s): Yamada N, Asano Y, Fujita M, Yamazaki S, Inanobe A, Matsuura N, Kobayashi H, Ohno S, Ebana Y, Tsukamoto O, Ishino S, Takuwa A, Kioka H, Yamashita T, Hashimoto N, Zankov DP, Shimizu A, Asakura M, Asanuma H, Kato H, Nishida Y, Miyashita Y, Shinomiya H, Naiki N, Hayashi K, Makiyama T, Ogita H, et al.
  • Journal Title: Circulation Volume: In press Issue: 18 Pages: 2157-2169
  • DOI: 10.1161/circulationaha.118.036761
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells (2019)
  • Author(s): Yoshinaga Daisuke、Baba Shiro、Makiyama Takeru、Shibata Hirofumi、Hirata Takuya、Akagi Kentaro、Matsuda Koichi、Kohjitani Hirohiko、Wuriyanghai Yimin、Umeda Katsutsugu、Yamamoto Yuta、Conklin Bruce R.、Horie Minoru、Takita Junko、Heike Toshio
  • Journal Title: Stem Cell Reports Volume: 13 Issue: 2 Pages: 394-404
  • DOI: 10.1016/j.stemcr.2019.06.007
  • NAID: 120006786948
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A de novo gain-of-function KCND3 mutation in early repolarization syndrome (2019)
  • Author(s): Takayama Koichiro、Ohno Seiko、Ding Wei-Guang、Ashihara Takashi、Fukumoto Daisuke、Wada Yuko、Makiyama Takeru、Kise Hiroaki、Hoshiai Minako、Matsuura Hiroshi、Horie Minoru
  • Journal Title: Heart Rhythm Volume: 16 Issue: 11 Pages: 1698-1706
  • DOI: 10.1016/j.hrthm.2019.05.033
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association of Genetic and Clinical Aspects of Congenital Long QT Syndrome With Life-Threatening Arrhythmias in Japanese Patients (2019)
  • Author(s): Shimizu Wataru、Makimoto Hisaki、Yamagata Kenichiro、Kamakura Tsukasa、Wada Mitsuru、Miyamoto Koji、Inoue-Yamada Yuko、Okamura Hideo、Ishibashi Kohei、Noda Takashi、Nagase Satoshi、Miyazaki Aya、Sakaguchi Heima、Shiraishi Isao、Makiyama Takeru、Ohno Seiko、Itoh Hideki、et al
  • Journal Title: JAMA Cardiology Volume: in press Issue: 3 Pages: 246-254
  • DOI: 10.1001/jamacardio.2018.4925
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pinacidil, a KATP channel opener, stimulates cardiac Na+/Ca2+ exchanger function through the NO/cGMP/PKG signaling pathway in guinea pig cardiac ventricular myocytes. (2019)
  • Author(s): Iguchi K, Saotome M, Yamashita K, Hasan P, Sasaki M, Maekawa Y, Watanabe Y.
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol. Volume: 392 Issue: 8 Pages: 949-959
  • DOI: 10.1007/s00210-019-01642-1
  • Peer Reviewed
• [Journal Article] Clinical Manifestations and Long-Term Mortality in <i>Lamin A/C</i> Mutation Carriers From a Japanese Multicenter Registry (2018)
  • Author(s): 14.Nakajima K, Aiba T, Makiyama T, Nishiuchi S, Ohno S, Kato K, Yamamoto Y, Doi T, Shizuta S, Onoue K, Yagihara N, Ishikawa T, Watanabe I, Kawakami H, Oginosawa Y, Murakoshi N, Nogami A, Aonuma K, Saito Y, Kimura T, Yasuda S, Makita N, Shimizu W, Horie M, Kusano K.
  • Journal Title: Circulation Journal Volume: 82 Issue: 11 Pages: 2707-2714
  • DOI: 10.1253/circj.CJ-18-0339
  • NAID: 40021697176
  • ISSN: 1346-9843, 1347-4820
  • Year and Date: 2018-10-25
  • Peer Reviewed / Open Access
• [Journal Article] HCN4 Gene Polymorphisms Are Associated With Occurrence of Tachycardia-Induced Cardiomyopathy in Patients With Atrial Fibrillation (2018)
  • Author(s): Nakano Yukiko、Ochi Hidenori、Sairaku Akinori、Onohara Yuko、Tokuyama Takehito、Motoda Chikaaki、Matsumura Hiroya、Tomomori Shunsuke、Amioka Michitaka、Hironobe Naoya、Ohkubo Yousaku、Okamura Shou、Makita Naomasa、Yoshida Yukihiko、Chayama Kazuaki、Kihara Yasuki
  • Journal Title: Circulation: Genomic and Precision Medicine Volume: 11 Issue: 7 Pages: 1-7
  • DOI: 10.1161/circgen.117.001980
  • Peer Reviewed
• [Journal Article] HCN4 pacemaker channels attenuate the parasympathetic response and stabilize the spontaneous firing of the sinoatrial node (2018)
  • Author(s): Kozasa Yuko、Nakashima Noriyuki、Ito Masayuki、Ishikawa Taisuke、Kimoto Hiroki、Ushijima Kazuo、Makita Naomasa、Takano Makoto
  • Journal Title: The Journal of Physiology Volume: 596 Issue: 5 Pages: 809-825
  • DOI: 10.1113/jp275303
  • Peer Reviewed / Open Access
• [Journal Article] Lack of genotype-phenotype correlation in Brugada Syndrome and Sudden Arrhythmic Death Syndrome families with reported pathogenic SCN1B variants (2018)
  • Author(s): Gray B., Hasdemir C., Ingles J., Aiba T., Makita N., Probst V., Wilde A. A. M., Newbury-Ecob R., Sheppard M. N., Semsarian C., Sy R. W., Behr E. R.
  • Journal Title: Heart Rhythm Volume: 15 Issue: 7 Pages: 1051-1057
  • DOI: 10.1016/j.hrthm.2018.03.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cardioprotective and functional effects of levosimendan and milrinone in mice with cecal ligation and puncture-induced sepsis (2018)
  • Author(s): Yamashita S, Suzuki T, Iguchi K, Sakamoto T, Tomita K, Yokoo H, Sakai M, Misawa H, Hattori K, Nagata T, Watanabe Y, Matsuda N, Yoshimura N, Hattori Y
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol Volume: 391 Issue: 9 Pages: 1021-1032
  • DOI: 10.1007/s00210-018-1527-z
  • Peer Reviewed
• [Journal Article] Complex aberrant splicing in the induced pluripotent stem cell?derived cardiomyocytes from a patient with long QT syndrome carrying KCNQ1-A344Aspl mutation (2018)
  • Author(s): Wuriyanghai Yimin、Makiyama Takeru、Sasaki Kenichi、Kamakura Tsukasa、Yamamoto Yuta、Hayano Mamoru、Harita Takeshi、Nishiuchi Suguru、Chen Jiarong、Kohjitani Hirohiko、Hirose Sayako、Yokoi Fumika、Gao Jingshan、Chonabayashi Kazuhisa、Watanabe Ken、Ohno Seiko、Yoshida Yoshinori、Kimura Takeshi、Horie Minoru
  • Journal Title: Heart Rhythm Volume: 15 Issue: 10 Pages: 1566-1574
  • DOI: 10.1016/j.hrthm.2018.05.028
  • Peer Reviewed / Open Access
• [Presentation] Modeling Inherited Arrhythmias Associated with Ryanodine Receptor Dysfunction using iPS Cells (2021)
  • Author(s): Makiyama T, Gao J, Yamamoto Y, Ishikawa T, Makita N, Kimura T et al.
  • Organizer: 第85回日本循環器学会学術集会
  • Int'l Joint Research
• [Presentation] Development of an Oligonucleotide-Based Therapeutic Approach for Calmodulinopathy (2021)
  • Author(s): Makiyama T, Yamamoto Y, Ishikawa T, Makita N, Horie M, Kimura T et al.
  • Organizer: 第85回日本循環器学会学術集会
  • Int'l Joint Research
• [Presentation] Genetic Risks of Sudden Death in Brugada Syndrome (2020)
  • Author(s): Makita N.
  • Organizer: 第84回日本循環器学会学術集会
  • Int'l Joint Research / Invited
• [Presentation] iPS Cell-Based Inherited Arrhythmia Disease Modeling. iPSCs: Limitations and Future challenges. Cardiac Arrhythmias (2020)
  • Author(s): Makiyama T.
  • Organizer: 第84回日本循環器学会学術集会
  • Int'l Joint Research
• [Presentation] Cardiac Emerinopathy, Novel Non-syndromic X-linked Left Ventricular Noncompaction Associated with Progressive Atrial Conduction Disturbance (2020)
  • Author(s): Ishikawa T, Aiba T, Ohno S, Yoshiura K, Makita N et al
  • Organizer: 第84回日本循環器学会学術集会
  • Int'l Joint Research
• [Presentation] Preclinical Proof-of-Concept Study: Antisense-mediated Knockdown of CALM as a Therapeutic Strategy for Calmodulinopathy (2020)
  • Author(s): Yamamoto Y, Makiyama T, Kondo Y, Iriyama Y, Ishikawa R, Kimura T et al.
  • Organizer: European Society of Cardiology Congress 2020
  • Int'l Joint Research
• [Presentation] Inhibition of Late Sodium Current Attenuates Prolonged Action Potential Durations in CACNA1C-E1115K iPS Cell Model with Disrupted Cav1.2 ion Selectivity (2020)
  • Author(s): Kashiwa A, Makiyama T, Ishikawa T, Makita N, Kimura T et al.
  • Organizer: The Heart Rhythm Society's Annual Scientific Sessions 2020
  • Int'l Joint Research
• [Presentation] Clinical and genetic aspects of pediatric Brugada syndrome (2020)
  • Author(s): Imamura T, Makiyama T, Sumitomo N, Horie M et al.
  • Organizer: European Society of Cardiology Congress 2020
  • Int'l Joint Research
• [Presentation] Cardiac Emerinopathy, Novel Non-syndromic X-linked Left Ventricular Noncompaction Associated with Progressive Atrial Conduction Disturbance (2020)
  • Author(s): Ishikawa T, Aiba T, Ohno S, Yoshiura K, Makita N et al.
  • Organizer: 第84回日本循環器学会学術集会
  • Int'l Joint Research
• [Presentation] 患者由来iPS 細胞モデルを用いたCav 1.2イオン選択性を障害するCACNA1C-E1115K変異がQT 延長症候群、Brugada 症候群を発症するメカニズムの解析 (2020)
  • Author(s): 柏麻美, 牧山武, 糀谷泰, マウリッセン トーマス, ウリヤンハイ イミン, 山本雄大, ガオ ジンシャン, ファン ハイ, 今村知彦, 相澤卓範, 石川泰輔, 大野聖子, 豊田太, 佐藤誠一, 高橋一浩, ウォルツェン クヌート, 堀江稔, 蒔田直昌, 木村剛
  • Organizer: 第97回日本生理学会大会
• [Presentation] 進行性心臓伝導障害患者の網羅的遺伝子解析とin vitro 機能解析系を用いて明らかになった心臓コネキシン病の分子病態 (2020)
  • Author(s): 石川泰輔, 関明子, 蒔田直昌
  • Organizer: 第97回日本生理学会大会
• [Presentation] Single Cell Electrophysiological Analysis of iPS Cell-Derived Cardiomyocytes Generated from Long-QT Syndrome Patients Carrying a CALM2 Mutation Using a Membrane Potential Imaging System (2019)
  • Author(s): Yamamoto Y, Makiyama T, Wuriyanghai Y, Kohjitani H, Hirose S, Gao J, Kashiwa A, Huang H, Ishikawa T, Ohno S, Chonabayashi K, Suda K, Yoshida Y, Horie M, Makita N, Kimura T.
  • Organizer: 第66回日本不整脈心電学会
• [Presentation] Genetic of cardiac conduction disturbance (2019)
  • Author(s): Makita N.
  • Organizer: 12th Asia Pacific heart Rhythm Society Scientific Session
  • Int'l Joint Research / Invited
• [Presentation] A case report of juvenile onset familial atrial fibrillation due to genetic defect (2019)
  • Author(s): Kumanomidou J, Makita N.
  • Organizer: 12th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research
• [Presentation] Modeling Overlap Syndrome of Long QT and Brugada Syndrome Associated with Induced Pluripotent Stem Cells (2019)
  • Author(s): Kashiwa A, Makiyama T, Kohjitani H, Wuriyanghai Y, Yamamoto Y, Jungshan G, Huang H, Ishikawa T, Ohno S, Toyoda F, Sato S, Horie M, Makita N, Kimura T.
  • Organizer: The 40th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Novel common genetic predispositions to sudden death in Brugada syndrome distinct from those to diagnostic Brugada-ECG pattern (2019)
  • Author(s): Ishikawa T.
  • Organizer: 第3回日本循環器病学会基礎研究フォーラム
  • Invited
• [Presentation] Modeling Overlapping Phenotypes of Long-QT and Brugada Syndrome with CACNA1C-E1115K that Alters Ion Selectivity of the Cardiac L-Type Calcium Channel Using Induced Pluripotent Stem Cells (2019)
  • Author(s): Kashiwa A, Makiyama T, Kohjitani H, Hirose S, Gao J, Kashiwa A, Huang H, Ishikawa T, Ohno S, Chonabayashi K, Suda K, Yoshida Y, Horie M, Makita N, Kimura T.
  • Organizer: 第66回日本不整脈心電学会
• [Presentation] Automatic Computational Action Potential Analyzing System Enables Accurate Drug Response Interpretation and Prediction in Human Induced Pluripotent Stem Cell-derived Cardiomyocytes (2019)
  • Author(s): Ishikawa T, Makita N.
  • Organizer: 第66回日本不整脈心電学会
• [Presentation] 40th Heart Rhythm Scientific Sessions (2019)
  • Author(s): Kohitani H, Kouda S, Himeno Y, Makiyama T, Kashiwa A, Wuriyanghai Y, Ymamaoto Y, Hiroe M, Kimura T, Noma A, Amano A.
  • Organizer: 40th Heart Rhythm Scientific Sessions
  • Int'l Joint Research
• [Presentation] Cardiac Emerinopathy, Novel Nonsyndromic Xlinked Left Ventricular Noncompaction Associated With Progressive Atrial Conduction Disturbance (2019)
  • Author(s): Ishikawa T, Barc J, Mishima H, Hirono K, Terada S, Kowase S, Sato T, Mukai Y, Yui Y, Ohkubo K, Kimoto H, Hata Y, Aiba T, Ohno S, Chishaki A, Shimizu W, Horie M, Ichida F, Nogami A, Yoshiura K, Schott JJ, Makita N.
  • Organizer: The 40th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Brugada症候群の予後予測に対する分子生理学的アプローチ (2019)
  • Author(s): 石川泰輔, 蒔田直昌
  • Organizer: 第67回日本心臓病学会学術集会
• [Presentation] 発症前診断を行ったQT延長症候群の姉妹 (2019)
  • Author(s): 松下悠紀, 永田弾尺, 小川昌宣, 鹿田佐和子, 石川泰輔, 蒔田直昌
  • Organizer: 日本人類遺伝学会第64回大会
• [Presentation] 次世代シーケンス解析による特発性心室細動の遺伝背景の解明 (2019)
  • Author(s): 謝颯琴, 高橋篤, 大野聖子, 鎌倉令, 石川泰輔, 植田初江, 斯波真理子, 南野直人, 林研至, 八木原伸江, 中野由紀子, 伊藤英樹, 宮本恵宏, 田中敏博, 蒔田直昌, 堀江稔, 清水渉, 草野研吾, 安田聡, 相庭武司
  • Organizer: 日本人類遺伝学会第64回大会
• [Presentation] 若年発症の家族性心房細動症例で遺伝子異常を認めた1例 (2019)
  • Author(s): 岩橋英明, 熊埜御堂淳, 高橋淳彌, 大渕綾, 本多亮博, 平方佐季, 大塚昌紀, 大江征嗣, 蒔田直昌, 福本義弘
  • Organizer: 日本内科学会 第326回九州地方会
• [Presentation] Structural Insights into Catecholaminergic Polymorphic Ventricular Tachycardia-associated RyR2 Mutant Channels using a Three-dimensional In Silico Model (2019)
  • Author(s): Gao J, Makiyama T, Ohno S, Yamamoto Y, Wuriyanghai Y, Kohjitani H, Kashiwa A, Huang H, Katou K, Horie M, Kimura T.
  • Organizer: European Society of Cardiology Congress 2019
  • Int'l Joint Research
• [Presentation] KCNQ1 Channel Dysfunction in human induced pluripotent stem cell-derived cardiomyocytes carrying a CALM2-N98S mutation associated with long QT syndrome (2019)
  • Author(s): Yamamoto Y, Makiyama T, Ishiakawa T, Makita N, Kimura T et al.
  • Organizer: American Heart Association Scientific Sessions 2019
  • Int'l Joint Research
• [Presentation] Morphological and electrophysiological characterization of homozygous lamin A/C knockout human induced-pluripotent stem cell-derived cardiomyocytes. American Heart Association Scientific Sessions 2019 (2019)
  • Author(s): Wuriyanghai Y, Makiyama T, Yamamoto Y, Horie M, Kimura T.
  • Organizer: American Heart Association Scientific Sessions 2019
  • Int'l Joint Research
• [Presentation] Single Cell Electrophysiological Analysis of iPS Cell-Derived Cardiomyocytes Generated from Long-QT Syndrome Patients Carrying a CALM2 Mutation Using a Membrane Potential Imaging System (2019)
  • Author(s): Yamamoto Y., Makiyama T., Wuriyanghai Y., Kohjitani H., Hirose S., Gao J., Kashiwa A., Huang H., Ishikawa T., Ohno S., Chonabayashi K., Suda K., Yoshida Y., Horie M., Makita N., Kimura T.
  • Organizer: 第66回日本不整脈心電学会
• [Presentation] Comprehensive Analyses Using Functional Evaluation and Whole-exome Sequencings to Decipher the Genetic predispositions for Sudden Death in Brugada Syndrome (2019)
  • Author(s): Makita N., Ishikawa T., Japanese Brugada Exome Consortium Investigators
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] Modeling Overlap Syndrome of Long QT and Brugada Syndrome Associated with Induced Pluripotent Stem Cells (2019)
  • Author(s): Kashiwa A., Makiyama T., Kohjitani H., Wuriyanghai Y., Yamamoto Y., Jungshan G., Huang H., Ishikawa T., Ohno S., Toyoda F., Sato S., Horie M., Makita N., Kimura T.
  • Organizer: The 40th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Modeling Overlapping Phenotypes of Long-QT and Brugada Syndrome with CACNA1C-E1115K that Alters Ion Selectivity of the Cardiac L-Type Calcium Channel Using Induced Pluripotent Stem Cells (2019)
  • Author(s): Kashiwa A., Makiyama T., Kohjitani H., Hirose S., Gao J., Kashiwa A., Huang H., Ishikawa T., Ohno S., Chonabayashi K., Suda K., Yoshida Y., Horie M., Makita N., Kimura T.
  • Organizer: 第66回日本不整脈心電学会
• [Presentation] Whole-Exome Sequencing to Discover Novel Responsible Genes for Brugada Syndrome: A Multicenter Japanese Registry (2019)
  • Author(s): Ishikawa T., Makita N., Japanese Brugada Exome project
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] Functional Reappraisal of SCN5A Mutations Reemphasize Their Predictive Value for Lethal Cardiac Events in Brugada Syndrome (2019)
  • Author(s): Ishikawa T., Makita N.
  • Organizer: 第66回日本不整脈心電学会
• [Presentation] Functional reappraisal of SCN5A mutations reemphasize their predictive value for lethal cardiac events in Brugada syndrome (2019)
  • Author(s): Ishikawa T., Kimoto H., Yamagata K., Asakura K., Aiba T., Kusano K., Shimizu W., Makita N.
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] Cardiac Emerinopathy, Novel Nonsyndromic Xlinked Left Ventricular Noncompaction Associated With Progressive Atrial Conduction Disturbance (2019)
  • Author(s): Ishikawa T., Barc J., Mishima H., Hirono K., Terada S., Kowase S., Sato T., Mukai Y., Yui Y., Ohkubo K., Kimoto H., Hata Y., Aiba T., Ohno S., Chishaki A., Shimizu W., Horie M., Ichida F., Nogami A., Yoshiura K., Schott JJ., Makita N.
  • Organizer: The 40th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Genetic Background in Patients with Idiopathic Ventricular Fibrillation: Known Gene Screening from Whole Exome Analysis Data (2019)
  • Author(s): Aiba T., Cha, Pei-Chieng, Takahashi A., Ohno S., Kamakura T., Ishikawa T., Ishibashi-Ueda H., Harada-Shiba M., Minamino N., Hayashi K., Yagihara N., Nakano Y., Itoh H., Miyamoto Y., Tanaka T., Makita N., Horie M., Shimizu W., Kusano K., Yasuda S.
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] Brugada症候群の予後予測に対する分子生理学的アプローチ (2019)
  • Author(s): 石川泰輔, 蒔田直昌
  • Organizer: 第67回日本心臓病学会学術集会
• [Presentation] Genetics and Clinical Aspects of Congenital Long-QT Syndrome: Results from the Japanese Multicenter Registry (2019)
  • Author(s): 清水渉, 山形研一郎, 野田崇, 草野研吾, 宮崎文, 牧山武, 伊藤英樹, 大野聖子, 渡部裕, 林研至, 森田宏, 吉永正夫, 福田恵一, 宮本恵宏, 鎌倉史郎, 田中敏博, 住友直方, 萩原誠久, 蒔田直昌, 堀江稔, 相庭武司
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] 若年発症の家族性心房細動症例で遺伝子異常を認めた1例 (2019)
  • Author(s): 岩橋英明, 熊埜御堂淳, 高橋淳彌, 大渕綾, 本多亮博, 平方佐季, 大塚昌紀, 大江征嗣, 蒔田直昌, 福本義弘
  • Organizer: 日本内科学会 第326回九州地方会
• [Presentation] Structural Insights into Catecholaminergic Polymorphic Ventricular Tachycardia-associated RyR2 Mutant Channels using a Three-dimensional In Silico Model. (2019)
  • Author(s): Gao J., Makiyama T., Ohno S., YamamotoY., Wuriyanghai Y., Kohjitani H., Kashiwa A., Huang H., Katou K., Horie M., Kimura T.
  • Organizer: European Society of Cardiology Congress 2019
  • Int'l Joint Research
• [Presentation] Challenges of iPS cell-Based Disease Modeling and Therapeutic Approaches to Inherited Arrhythmias (2019)
  • Author(s): Makiyama T.
  • Organizer: 第3回日本循環器学会基礎研究フォーラム
  • Invited
• [Presentation] Automatic Computational Action Potential Analyzing System Enables Accurate Drug Response Interpretation and Prediction in Human Induced Pluripotent Stem Cell-derived Cardiomyocytes (2019)
  • Author(s): Kohjitani H., Kouda S., Himeno Y., Makiyama T., Kashiwa A., Wuriyanghai Y., Yamamoto Y., Horie M., Kimura T., Noma A., Amano A.
  • Organizer: The 40th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Algorithmic Auto-Recreation System of hiPSC-CMs Simulation and Prediction of Drug Testing (2019)
  • Author(s): Kohjitani H., Kouda S., Himeno Y., Makiyama T., Huang H., Gao J., Kashiwa A., Wuriyanghai Y., Yoshinaga D., Kimura T., Amano A.
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] Overlapping Sick Sinus Syndrome Associated with SCN5A Mutations in Patients with Brugada Syndrome (2019)
  • Author(s): Kashiwa A., Makiyama T., Ohno S., Aoki H., Kohjitani H., Wuriyanghai Y., Yamamoto Y., Gao J., Hai H., Horie M., Kimura T.
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] Three-Dimensional Structural Analysis of Mutant Ryanodine Receptor Type 2 Channels Associated with Catecholaminergic Polymorphic Ventricular Tachycardia (2019)
  • Author(s): Gao J., Makiyama T., Ohno S., Yamamoto Y., Wuriyanghai Y., Kohjitani H., Kashiwa A, Horie M., Kimura T.
  • Organizer: 第83回日本循環器学会学術集会
• [Presentation] 不整脈症候群で心臓が構造的に正常な患者における心筋症の原因遺伝子変異の特定 (2018)
  • Author(s): 八木原伸江, 渡部裕, 蒔田直昌, 堀江稔, 清水渉, 大野聖子, 長谷川奏恵, 相庭武司, 田中敏博, 角田達彦, 重水大智, 南野徹
  • Organizer: 第82回日本循環器学会学術集会
• [Presentation] CaMKII as a target for novel therapy to treat lethal ventricular tachyarrhythmias (2018)
  • Author(s): 辻幸臣, 蒔田直昌
  • Organizer: 第57回日本生体医工学学会大会
• [Presentation] CALM2関連性QT延長症候群iPSCモデルにおいて、l-cis-diltiazemは遅延したL型calciumチャネルの不活性化をさらに強める (2018)
  • Author(s): 横井文香, 牧山武, 山本雄大, 張田健志, Yimin Wuriyanghai, 早野護, 西内英, 糀谷泰彦, 廣瀬紗也子, 高景山, 大野聖子, 豊田太, 吉田善紀, 蒔田直昌, 堀江稔, 木村剛
  • Organizer: 第82回日本循環器学会学術集会
• [Presentation] Torsadogenic Action Of Late Na+ Current In Experimental Electrical Storm (2018)
  • Author(s): Yamazaki M., Tsuji Y. , Niwa R., Tomii N., Arafune T., Honjo H., Dobrev D., Nattel S., Kodama I., Sakuma I., Makita N.
  • Organizer: The 39th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Torsadogenic Action Of Late Na+ Current In Experimental Electrical Storm (2018)
  • Author(s): Yamamoto Y., Makiyama T, Wuriyanghai Y., Kohjitani H., Hirose S., Gao J, Kashiwa A., Kimura T., Ishikawa T., Motomura H., Makita N, Ohno S., Chonabayashi K., Yoshida Y., Suda K., Horie M
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research
• [Presentation] The inactivation of L-type Ca2+ Channel was impaired in Human iPS Cell Model of Long-QT Syndrome with CALM2-D134H Mutation. (2018)
  • Author(s): Yamamoto Y., Makiyama T., Harita T., Hayano M., Nishiuchi S., Wuriyanghai Y., Kohjitani H., Hirose S., Yokoi F., Ishikawa T., Ohno S., Chonabayashi K., Suda K., Yoshida Y., Horie M., Makita N., Kimura T.
  • Organizer: 第82回日本循環器学会学術集会
• [Presentation] Duration Predicts Events In Caucasians With E1784K-SCN5A: The E1784K International Consortium (2018)
  • Author(s): Wijeyeratne Y., Makita N., Roden D., Probst V., Ackerman M., Aiba T., Behr E. et al.
  • Organizer: The 39th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Mechanisms of Electrical Storm Associated With QT Prolongation: Successful Mapping of Torsades de Pointes in Rabbits (2018)
  • Author(s): Tsuji Y., Yamazaki M., Niwa R., Tomii N., Arafune T., Honjo H., Dobrev D., Nattel S., Kodama I., Sakuma I., Makita N
  • Organizer: American Heart Association Annual Scientific Sessions 2018
  • Int'l Joint Research
• [Presentation] HCN4 Polymorphisms and Tachycardia Induced Cardiomyopathy (2018)
  • Author(s): Nakano Y., Ochi H., Onohara Y., Sairaku A., Tokuyama T., Motoda C., Matsumura H., Tomomori S., Amioka M., Hironobe N., Ohkubo Y., Okamura S., Makita N., Yoshida Y., Chayama K., Kihara Y.
  • Organizer: The 65th Annual Meeting of the Japan Heart Rhythm Society
• [Presentation] Clinical Manifestations and Long-term Mortality in Lamin A/C Mutation Carriers from the Japanese Multicenter Registry (2018)
  • Author(s): Nakajima K., Aiba T., Makiyama T., Nishiuchi S., Ohno S., Doi T., Shizuta S., Onoue K., Yagihara N., Ishikawa T., Watanabe I., Oginosawa Y., Nogami A., Aonuma K., Saitoh Y., Kimura T., Makita N., Shimizu W., Horie M., Kusano K.
  • Organizer: The 65th Annual Meeting of the Japan Heart Rhythm Society
• [Presentation] Genotype-Dependent Differences in Short QT Syndrome (2018)
  • Author(s): Makita N.
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research / Invited
• [Presentation] Atrial Conduction Defects Caused by a Connexin45 Mutation (2018)
  • Author(s): Makita N.
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research / Invited
• [Presentation] Novel Arrhythmia Syndrome Associated with Gap Junction Mutations (2018)
  • Author(s): Makita N.
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research / Invited
• [Presentation] Clinical and Genetic Basis of Calmodulinopathy (2018)
  • Author(s): Makita N.
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research / Invited
• [Presentation] Pathophysiology of Inherited Arrhythmias Associated with Ca Handling Abnormality (2018)
  • Author(s): Makita N.
  • Organizer: The 65th Annual Meeting of the Japan Heart Rhythm Society
  • Int'l Joint Research / Invited
• [Presentation] Mutation Hot-spot-based Risk Stratification In Long QT Syndrome Type 1: Data From A Nationwide Japanese LQT Registry (2018)
  • Author(s): Kashiwa A., Aiba T., Makimoto H., Yamagata K. , Keiko S. , Yagihara N., Watanabe H. , Ohno S., Hayashi K. , Sumitomo N., Yoshinaga M. , Morita H., Miyamoto Y. , Makita N. , Horie M., Yasuda S., Kusano K., Shimizu W. , The_Japanese_LQTS_registry_investigators.
  • Organizer: The 39th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] Cardiac Connexin Syndrome: A New Inherited Cardiac Conduction Disease Entity with Distinct Electrophysiological and Extracardiac Manifestations. (2018)
  • Author(s): Ishikawa T., Seki A., Hagiwara N., Makita N.
  • Organizer: 第82回日本循環器学会学術大会
• [Presentation] Rare Coding Variants in Genes Other Than SCN5A Are Minimal Genetic Burden on the Prognosis of Brugada Syndrome (2018)
  • Author(s): Ishikawa T., Mishima H., Ohno S., T. Aiba, Nakano Y., Aizawa Y., Nakajima T., Hayashi K., Murakoshi N., Yagihara N., Kimoto H., Makiyama T., Watanabe H., Morita H., Yoshiura K., Nogami A., Shimizu W., Horie M., Tanaka T., Makita N.
  • Organizer: The 65th Annual Meeting of the Japan Heart Rhythm Society
• [Presentation] Lack Of Genotype-phenotype Correlation In Brugada Syndrome And Sudden Arrhythmic Death Syndrome Families With Reported Pathogenic SCN1B Variants (2018)
  • Author(s): Gray B. , Hasdemir C. , Ingles J. , Aiba T., Makita N., Probst V., Wilde A., Semsarian C., Sy R, Behr E.
  • Organizer: The 39th Heart Rhythm Society Scientific Sessions
  • Int'l Joint Research
• [Presentation] The effects of pinacidil, an ATP-sensitive K+ channel opener on cardiac Na＋/Ca&sup2;＋ exchanger function in guinea pig cardiomyocytes. (2018)
  • Author(s): Iguchi K, Saotome M, Yamashita K, Ikoma T, Hasan P, Maekawa Y, Watanabe Y.
  • Organizer: 63rd Annual Meeting of the Biophysical Society
  • Int'l Joint Research
• [Presentation] Pinacidil, a KATP channel opener stimulate a cardiac Na＋/Ca&sup2;＋ exchanger function through NO/cGMP/PKG pathways in guinea-pig cardiac myocytes (2018)
  • Author(s): Iguchi K, Watanabe Y, Saotome M, Yamashita K, Sasaki M, Maekawa Y
  • Organizer: 18th World Congress of Basic and Clinical Pharmacology
  • Int'l Joint Research / Invited
• [Presentation] 盲腸結紮穿刺誘発性敗血症モデルマウスにおけるレボシメンダンおよびミルリノンの強心作用と心保護効果 (2018)
  • Author(s): 冨田賢吾, 山下重幸, 鈴木登紀子, 坂本卓也, 酒井麻里, 横尾宏毅, 渡邊泰秀, 芳村直樹, 服部裕一
  • Organizer: 第47回日本心脈管作動物質学会年会
• [Presentation] モルモット心筋においてピナシジルはNa+/Ca2+ exchangerをNO/cGMP/PKG経路を介して増強させる (2018)
  • Author(s): 井口恵介, 早乙女雅夫, 山下寛奈, 前川裕一郎, 渡邊泰秀
  • Organizer: 第28回日本循環薬理学会
• [Presentation] Can patient-derived iPSCs be applied to phenotypic cell-based high throughput screening assessment to distinguish between different types of long-QT syndrome? (2018)
  • Author(s): Yoshinaga D, Baba S, Shibata H, Hirata T, Makiyama T., Nishikomori R.
  • Organizer: European Society of Cardiology Congress 2018
  • Int'l Joint Research
• [Presentation] Significance of cell-specific precise computer simulation using new mathematical models of human induced pluripotent stem cell derived cardiomyocyte in drug testing (2018)
  • Author(s): Kohjitani H., Kouda S., Himeno Y., Makiyama T., Yokoi F., Hirose S., Wuriyanghai Y., Yamamoto Y., Horie M., Kimura T., Noma A., Amano A.
  • Organizer: European Society of Cardiology Congress 2018
  • Int'l Joint Research
• [Presentation] Analysis of Disease-Causing Mechanism of Lamin A/C-Related Cardiomyopathy Using Homozygous Lamin A/C Knock out Human Induced Pluripotent Stem Cell Model (2018)
  • Author(s): Wuriyanghai Y., Makiyama T., Yamamoto Y., Nishiuchi S., Hirose S., Kohjitani H., Gao J., Kashiwa A., Ohno S., Horie M., Kimura T.
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research
• [Presentation] Three-dimensional Structural Analysis of Mutant Ryanodine Receptor Type 2 Channels Associated with Catecolaminergic Polymorphic Ventricular Tachycardia (2018)
  • Author(s): Gao J., Makiyama T., Ohno S., Yamamoto Y., Wuriyanghai Y., Kohjitani H., Kashiwa A., Hirose S., Horie M., Kimura T.
  • Organizer: The 11th Asia Pacific Heart Rhythm Society Scientific Session
  • Int'l Joint Research
• [Presentation] Symposium 5: Sudden Cardiac Death─Elucidation of Pathogenesis, Prediction, and Prevention─: Induced pluripotent stem cell-based modeling of inherited arrhythmias (2018)
  • Author(s): 牧山 武
  • Organizer: 第82回日本循環器学会学術集会
• [Presentation] Modelling long-QT syndrome with human iPS cells and development of new pharmacotherapies (2018)
  • Author(s): Makiyama T.
  • Organizer: 23rd International Society of Cardiovascular Pharmacotherapy
  • Int'l Joint Research / Invited
• [Presentation] Human induced pluripotent stem cell models for inherited arrhythmias (2018)
  • Author(s): Makiyama T.
  • Organizer: The 2018 International Congress on Electrocardiology
  • Int'l Joint Research / Invited
• [Presentation] Modelling calmodulin-related long-QT syndrome using human iPS cells: therapeutic genome editing using CRISPR/Cas9 system (2018)
  • Author(s): Makiyama T.
  • Organizer: 第65回日本不整脈心電学会学術大会
  • Invited
• [Book] ゼロから学ぶ Burugada症候群 (2020)
  • Author(s): 石川泰輔, 蒔田直昌
  • Total Pages: 133
  • Publisher: 大道学館出版部
• [Book] コメディカルのための薬理学（第3版） (2018)
  • Author(s): 渡邊泰秀, 安西尚彦, 櫻田香
  • Total Pages: 264
  • Publisher: 朝倉書店
  • ISBN: 9784254330106
• [Remarks] 国立循環器病研究センター 創薬オミックス解析センター 研究プロジェクト
  • URL: http://www.ncvc.go.jp/omics/research/project03.html
• [Remarks] 国立循環器病研究センター 創薬オミックス解析センター ホームページ
  • URL: http://www.ncvc.go.jp/omics/research/project03.html