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Single cell analysis of cell diversity during skin wound healing

Research Project

Project/Area Number 18H02962
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 56070:Plastic and reconstructive surgery-related
Research InstitutionNagasaki University

Principal Investigator

TANAKA Katsuya  長崎大学, 医歯薬学総合研究科(医学系), 研究協力員 (70722750)

Co-Investigator(Kenkyū-buntansha) 森 亮一  長崎大学, 医歯薬学総合研究科(医学系), 准教授 (30509310)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords再生医学 / 発生 / 発現制御 / 病理学 / 組織 / 細胞 / 分化 / 発生・分化 / 組織・細胞 / 皮膚創傷治癒 / 1細胞 / 遺伝子発現 / microRNA
Outline of Final Research Achievements

The skin wound healing process consists of an inflammatory phase, a proliferative phase, and a maturation phase. In recent years, functions of microRNAs have been shown to play important roles in wound healing, scarring, and in the response to infection. Various cell types are involved in the initiation of scarring; however, analysis of skin wound healing at the single cell level has not been reported. In this study, we established spatial transcriptome analysis and performed functional analysis. We then identified inflammation-related genes. We believe that the results of this research will lead to the elucidation of the molecular mechanisms that underlie new cell-cell interactions and cell differentiation during skin wound healing.

Academic Significance and Societal Importance of the Research Achievements

本研究では、炎症反応を基軸とした組織修復過程の全容を解明するため、創傷治癒研究に資する空間的トランスクリプトーム解析法の確立をめざした。そして、新規瘢痕形成関与遺伝子を同定した。本研究成果によって、シングルセル解析の問題点であった「採取した細胞の位置情報の欠落」を克服することができた。今後は、本研究成果を発展させて、未病状態における病態発症リスクを予想することができる技術開発にも取り組みたいと考えている。そして、治療法開発だけでなく真の予防法の確立に繋がるように研究を進めて、健康長寿社会の実現に寄与する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (21 results)

All 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] University of Bristol/King’s College London(英国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Does Calorie Restriction Modulate Inflammaging via FoxO Transcription Factors?2020

    • Author(s)
      Kim Sang-Eun、Mori Ryoichi、Shimokawa Isao
    • Journal Title

      Nutrients

      Volume: 12 Issue: 7 Pages: 1959-1959

    • DOI

      10.3390/nu12071959

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of rikkunshito supplementation on mice: analysis of oxidative stress resistance and lifespan2020

    • Author(s)
      Wang Z, Komatsu T, Ohata Y, Watanabe Y, Yuan Y, Yoshii Y, Park S, Mori R, Satou M, Kondo Y, Shimokawa I, Chiba T
    • Journal Title

      Geriatrics and Gerontology International

      Volume: 20 Issue: 3 Pages: 238-247

    • DOI

      10.1111/ggi.13848

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 老化の分子シグナル2020

    • Author(s)
      森亮一,下川功
    • Journal Title

      医学のあゆみ

      Volume: 272 Pages: 619-624

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Identification of Specific miRNAs in Neutrophils of Type 2 Diabetic Mice: Overexpression of miRNA-129-2-3p Accelerates Diabetic Wound Healing.2019

    • Author(s)
      Umehara T, Mori R, Mace KA, Murase T, Abe Y, Yamamoto T, Ikematsu K
    • Journal Title

      Diabetes

      Volume: 68 Issue: 3 Pages: 617-630

    • DOI

      10.2337/db18-0313

    • NAID

      120006987788

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification and functional analysis of inflammation-related miRNAs in skin wound repair2018

    • Author(s)
      Mori Ryoichi、Tanaka Katsuya、Shimokawa Isao
    • Journal Title

      Development, Growth & Differentiation

      Volume: 60 Issue: 6 Pages: 306-315

    • DOI

      10.1111/dgd.12542

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Targeting miR-223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds2018

    • Author(s)
      Yayoi Kawano, Takehisa Hanawa, Rina Takahashi et al.
    • Journal Title

      EMBO Molecular Medicine

      Volume: 2018 Issue: 10 Pages: 1-21

    • DOI

      10.15252/emmm.201809024

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] miR-142はsmall GTPaseを介した好中球細胞骨格制御による黄色ブドウ球菌感染創の改善に必須である2018

    • Author(s)
      森 亮一,田中 克弥,野崎 中成,下川 功
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 69 Pages: 46-52

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 栄養と加齢ー基礎研究よりー2018

    • Author(s)
      森 亮一,朴 盛浚,下川 功
    • Journal Title

      栄養

      Volume: 3 Pages: 233-237

    • Related Report
      2018 Annual Research Report
  • [Presentation] 皮膚創傷治癒における miRNA 機能と核酸医薬への応用2020

    • Author(s)
      森 亮一
    • Organizer
      第50回 日本創傷治癒学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 組織特異的 miR-142 ノックアウトマウスを用いた生命恒常性維持機構の解明2019

    • Author(s)
      森亮一、小松利光、朴盛浚、林洋子、下川功
    • Organizer
      第42回日本基礎老化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Inflammation-related microRNAs regulate neutrophilic functions at S. aureus-infected skin wound sites2019

    • Author(s)
      Ryoichi Mori
    • Organizer
      The 3rd Joint Symposium between Nagasaki University and Wurzburg University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 皮膚創傷治癒における炎症関連 miRNA の同定及び miR-223 の機能解析2019

    • Author(s)
      森亮一
    • Organizer
      長崎障害者支援再生医療研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 寿命及び代謝制御における炎症関連 miR-142 ファミリーの機能解析2019

    • Author(s)
      森亮一,朴盛浚,林洋子,下川功
    • Organizer
      第108回日本病理学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Attenuation of age-related fatty liver pathogenesis in miR-142-deficient mice2019

    • Author(s)
      Mori R, Tanaka K
    • Organizer
      Gordon Research Conferences-Tissue Repair & Regeneration
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] miRNA-223 発現抑制は Interleukin-6 分泌促進を介し黄色ブドウ球菌感染創を改善する2019

    • Author(s)
      森 亮一
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Comprehensive identification of wound healing and inflammation miRNAs reveals a key role for miR-223 in neutrophilic clearance of S.aureus at wound sites2018

    • Author(s)
      Mori R
    • Organizer
      EMBL Symposia The Complex Life of RNA
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of C/EBPα-miR-223-Interleukin-6 secretion pathway at Staphylococcus aureus-infected wound sites2018

    • Author(s)
      森 亮一
    • Organizer
      第107回日本病理学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 好中球由来 miR-223 の発現抑制は皮膚感染創における黄色ブドウ球菌排除を促進する2018

    • Author(s)
      森 亮一
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] miR-223 発現抑制は皮膚創傷部位の感染予防に効果的である2018

    • Author(s)
      森 亮一
    • Organizer
      第8回臨床ゲノム医療学会
    • Related Report
      2018 Annual Research Report
  • [Remarks] 長崎大学医学部病理学

    • URL

      http://www.med.nagasaki-u.ac.jp/pathlgy1/

    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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