• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Physiological substrates for masticatory rhythm generation during REM sleep

Research Project

Project/Area Number 18H02965
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 57010:Oral biological science-related
Research InstitutionOsaka University

Principal Investigator

加藤 隆史  大阪大学, 歯学研究科, 教授 (50367520)

Co-Investigator(Kenkyū-buntansha) 豊田 博紀  大阪大学, 歯学研究科, 准教授 (00432451)
片桐 綾乃  大阪大学, 歯学研究科, 講師 (40731899)
佐藤 元  大阪大学, 歯学研究科, 助教 (10432452)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Granted (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords睡眠時ブラキシズム / 顎運動 / 睡眠 / レム睡眠 / 咀嚼筋活動 / 大脳皮質 / 中脳 / 咀嚼運動 / 脳幹 / 脳波 / 咀嚼リズム / 咀嚼 / リズム発生機構 / 異常運動 / 運動抑制
Outline of Annual Research Achievements

本年度は、レム睡眠調節機構と咀嚼リズム発生機構との機能的な相関を調べるため、慢性実験と急性実験を用いて以下の動物実験を実施した。急性実験では、咀嚼筋活動や顎運動を記録ができる準備を施し、大脳皮質咀嚼野を連続電気刺激して、咀嚼様のリズミカルな顎運動を誘発した。そして、中脳や橋に刺激電極を刺入できるようにした。大脳皮質誘発性顎運動中に、短時間連続微小電気刺激を中脳尾側から橋吻側部の網様体に与え、その刺激による皮質誘発性顎運動の変化を刺激部位ごと調べた。その結果、皮質誘発性顎運動が一時的に停止する領域と、皮質誘発性顎運動が閉口優位もしくはリズム性が増加する領域が存在した。前者は、中脳尾側から橋吻側にかけた網様体に存在し、中脳尾側レベルでは内外に分布していた。刺激部位を組織学的に観察したところ、皮質誘発性の運動が停止を認めた領域は、吻側橋網様体や脚橋被蓋核を含む領域と考えられた。さらに、慢性実験では、大脳皮質および皮質誘発性顎運動を抑制する部位に刺激電極を留置し、自然咀嚼への刺激効果や、睡眠中のそれらの部位の脳波活動を記録するためのプロトコールを固めた。一方、ヒトの研究では、レム睡眠周期を含めた睡眠や脳波活動を解析し、新規環境への順応夜ではレム睡眠量や発生回数が減少すること、また順応夜ではリズミカルな咀嚼筋活動の発生が少ないことが分かった。さらに、レム睡眠の咀嚼筋活動の発生機序を解析する一環として、各睡眠段階における咀嚼筋の筋トーヌスや活動発現レベルを定量的に評価する解析方法として、クラスター分析の活用を試みた。

Current Status of Research Progress
Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

コロナ感染症に関連する活動自粛に加えて、実験の過程で複数回の麻酔下における外科的処置や回復、馴化トレーニングを実施するなど、実験のステップが複雑となったことで、想定よりも実験行程に日数がかかったため。

Strategy for Future Research Activity

大脳皮質や錘体路、皮質誘発性顎運動を抑制する部位に刺激電極を留置し、脳定位固定装置上で睡眠覚醒及び自然咀嚼ができる状態にして、咀嚼や皮質誘発性顎運動への刺激効果や、睡眠中の脳電位活動を記録する。刺激によって生じる脳波、筋トーヌス、心拍数などの変化を解析し、刺激効果が生じた部位の局在との関係を組織学的に検索する。ヒトを用いた実験では、ポリソムノグラフィー検査のデータを用いて、レム睡眠中に発生するリズミカルな咀嚼筋活動の発生に関与する要因を明らかにするため、睡眠中の咀嚼筋活動・脳波の定量的な解析を実施する。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(21 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 10 results,  Invited: 4 results) Book (3 results)

  • [Journal Article] Discrepancies in the time course of sleep stage dynamics, electroencephalographic activity and heart rate variability over sleep cycles in the adaptation night in healthy young adults.2021

    • Author(s)
      Shirota, A., M. Kamimura, A. Kishi, H. Adachi, M. Taniike, T. Kato.
    • Journal Title

      Frontiers in Physiology

      Volume: 12 Pages: 1-11

    • DOI

      10.3389/fphys.2021.623401

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Research routes on improved sleep bruxism metrics: Toward a standardised approach2021

    • Author(s)
      Lavigne Gilles、Kato Takafumi、Herrero Babiloni Alberto、Huynh Nelly、Dal Fabbro Cibele、Svensson Peter、Aarab Ghizlane、Ahlberg Jari、Baba Kazuyoshi、Carra Maria Clotilde、Cunha Thays Crosara A.、Gon?alves Daniela A. G.、Manfredini Daniele、Stuginski‐Barbosa Juliana、Wieckiewicz Mieszko、Lobbezoo Frank
    • Journal Title

      Journal of Sleep Research

      Volume: -

    • DOI

      10.1111/jsr.13320

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First night effect on polysomnographic sleep bruxism diagnosis varies among young subjects with different degrees of rhythmic masticatory muscle activity2020

    • Author(s)
      Haraki Shingo、Tsujisaka Akiko、Toyota Risa、Shiraishi Yuki、Adachi Hiroyoshi、Ishigaki Shoichi、Yatani Hirofumi、Taniike Masako、Kato Takafumi
    • Journal Title

      Sleep Medicine

      Volume: 75 Pages: 395-400

    • DOI

      10.1016/j.sleep.2020.08.012

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sleep stage dynamics in young patients with sleep bruxism2020

    • Author(s)
      Kishi Akifumi、Haraki Shingo、Toyota Risa、Shiraishi Yuki、Kamimura Mayo、Taniike Masako、Yatani Hirofumi、Kato Takafumi
    • Journal Title

      Transl Psychiatry

      Volume: 43 Pages: 1-12

    • DOI

      10.1093/sleep/zsz202

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Changes in oxygen and carbon dioxide in the genesis of sleep bruxism: a mechanism study2020

    • Author(s)
      Suzuki Y, Rompre P, Mayer P, Kato T, Okura K, Lavigne GJ
    • Journal Title

      J Prosthodont Res

      Volume: 64(1) Issue: 1 Pages: 43-47

    • DOI

      10.1016/j.jpor.2019.04.012

    • NAID

      130007845589

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Experimentally induced rhythmic jaw muscle activities during non-rapid eye movement sleep in freely moving guinea pigs.2019

    • Author(s)
      Yamada KI, Higashiyama M, Toyoda H, Masuda Y, Kogo M, Yoshida A, Kato T
    • Journal Title

      Journal of sleep research

      Volume: e12823

    • DOI

      10.1111/jsr.12823

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Pathophysiology of SB: challenges from human and animal studies.2020

    • Author(s)
      Kato T.
    • Organizer
      The 68th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Masseter Muscle Activity During REM Sleep in Sleep Bruxism2019

    • Author(s)
      R.Toyota, M.Okura, S.Haraki, A.Tsujisaka, H.Adachi, K.Ikebe, H.Yatani, T.Kato
    • Organizer
      97th.IADR General Session
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sleep Bruxism:An Update for dental Clinicians2019

    • Author(s)
      Takafumi Kato
    • Organizer
      ADA FDI World Dental Congress 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sleep Related Problems in Dentistry2019

    • Author(s)
      Takafumi Kato
    • Organizer
      ADA FDI World Dental Congress 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 睡眠時ブラキシズムの謎を解くために2019

    • Author(s)
      加藤隆史
    • Organizer
      第88回松本歯科大学学会(総会)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] The feature of sleep stage sequence of nocturnal REM periods reflects the pathophysiology of narcolepsy2019

    • Author(s)
      Mashita M, Adachi H, Koyama M, Nonoue S, Mamiya Y, Shigedo Y, Sugita Y, Kyotani K, Kato T, Ikeda M.
    • Organizer
      Worldsleep 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sleep stage dynamics in patients with sleep bruxism2018

    • Author(s)
      Kishi A, Kato T.
    • Organizer
      The 32nd Annual Meeting of the Associated Professional Sleep Societies, Baltimore, MD, USA, June 3-6
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Masseter muscle activity during REM sleep in young adults with sleep bruxism2018

    • Author(s)
      Risa Toyota, Mutsumi Okura, Shigeru Nonoue, Shingo Haraki, Akiko Tsujisaka, Hiroyoshi Adachi, Kazunori Ikebe, Takafumi Kato.
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Physiological characteristics of rhythmic masticatory muscle activity during sleep in children2018

    • Author(s)
      Yuki Shiraishi, Masaya Tachibana, Sheng-Yun Lu, Ai Shirota, Ikuko Mohri, Shingo Haraki, Atsuko Tsujisaka, Masako Taniike, Takashi Yamashiro, Takafumi Kato.
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Physiologic process before rhythmic jaw movements after ketamine injections in guinea pigs.2018

    • Author(s)
      Takafumi Kato, Yutaka Matsuura, Hiroshi Yano, Makoto Higashiyama, Hiroki Toyoda, Ayano Katagiri, Hajime Sato, Narikazu Uzawa, Atsushi Yoshida.
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ケタミン麻酔動物におけるリズム性顎運動発現の生理学的機序2018

    • Author(s)
      松裏豊,矢野浩司,豊田博紀,片桐綾乃,東山亮,鵜澤成一,吉田篤,加藤隆史.
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Time-course changes in cortical, cardiac and respiratory changes before spontaneous rhythmic jaw movements after repeated ketamine injections in anesthetized guinea pigs.2018

    • Author(s)
      Yutaka Matsuura, Hiroshi Yano, Hiroki Toyoda, Ayano Katagiri, Makoto Higashiyama, Hajime Sato, Narikazu Uzawa, Atsushi Yoshida, Takafumi Kato.
    • Organizer
      Oral Neuroscience 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Book] 最新臨床睡眠学(第2版)2020

    • Author(s)
      加藤隆史、豊田理紗、白石優季、原木真吾
    • Total Pages
      765
    • Publisher
      日本臨牀社
    • Related Report
      2020 Annual Research Report
  • [Book] Sleep Medicine For Dentists 2nd eds, Physiologic mechanisms associated with SB genesis2020

    • Author(s)
      Kato T, Okura K, Macaluso GM, Lavigne GJ
    • Total Pages
      224
    • Publisher
      Quintessence publishing
    • Related Report
      2019 Annual Research Report
  • [Book] 基礎歯科生理学第7版 第12章 高次脳機能 IV 睡眠2020

    • Author(s)
      加藤隆史
    • Total Pages
      475
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2021-12-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi