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Fluorescent imaging and analysis of molecular biological properties of the cells that contribute to the integration of the skeletal components

Research Project

Project/Area Number 18H02966
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 57010:Oral biological science-related
Research InstitutionHiroshima University

Principal Investigator

Shukunami Chisa  広島大学, 医系科学研究科(歯), 教授 (60303905)

Co-Investigator(Kenkyū-buntansha) 谷本 幸太郎  広島大学, 医系科学研究科(歯), 教授 (20322240)
三浦 重徳  東京大学, 生産技術研究所, 特任講師 (70511244)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords腱 / 靱帯 / 細胞分化 / 幹細胞 / Scleraxis / Sox9 / 縫合部 / Sxleraxis / Enthesis / 歯根 / 靭帯 / 顎関節
Outline of Final Research Achievements

In this study, taking advantage of ScxTomato;Sox9GFP mouse embryos, we analyzed the temporal and spatial localization changes of Scx-positive cells and Scx/Sox9-positive cells that contribute to the integration of skeletal components in the process of craniomaxillofacial formation. Using ScxGFP iPS cells established from fibroblasts derived from ScxGFP Tg mouse embryos, we developed the tenogenic and ligamentogenic differentiation system that faithfully reflects the in vivo process, and performed single cell RNA-seq. Dataset of scRNA-seq and transcriptome of Scx deficient tendon enabled identification of Scx-related genes in which tendon-related genes are enriched.

Academic Significance and Societal Importance of the Research Achievements

腱・靱帯は、硬組織と筋肉の連結の要となる組織である。とりわけ、硬組織と腱・靱帯の接合部は、運動機能を円滑に維持するために重要な部位である。本研究では、この接合部の形成に寄与する細胞集団の発生過程におけるダイナミックな局在変化を明らかにし、分子レベルで、その特性を解析した。これらの知見は、超高齢社会における我が国において、「寝たきりによる要介護状態」や「加齢」に伴う関節拘縮や筋萎縮等を予防・改善する治療法の確立に向けた基盤技術につながることが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(23 results)

All 2021 2020 2019 2018

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 6 results)

  • [Journal Article] Scleraxis upregulated by transforming growth factor-β1 signaling inhibits tension-induced osteoblast differentiation of priodontal ligament cells via ephrin A22021

    • Author(s)
      Kawatsu M, Takeshita N, Takimoto A, Yoshimoto Y, Seiryu M, Ito A, Kimura S, Kawamoto T, Hiraki Y, Shukunami C, Takano-Yamamoto T
    • Journal Title

      Bone

      Volume: 149 Pages: 115969-115969

    • DOI

      10.1016/j.bone.2021.115969

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Scx+/Sox9+ cells as potential progenitor cells for postnatal supraspinatus enthesis formation and healing after injury in mice2020

    • Author(s)
      Ideo K, Tokunaga T, Shukunami C, Takimoto A, Yoshimoto Y, Yonemitsu R, Karasugi T, Mizuta H, Hiraki Y, Miyamoto T
    • Journal Title

      PLOS ONe

      Volume: 15 Pages: 1-14

    • DOI

      10.1371/journal.pone.0242286

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 腱・靱帯研究のフロンティア(5)「腱・靱帯の特異的分子マーカー」2020

    • Author(s)
      西崎有利子, 宿南知佐
    • Journal Title

      整形・災害外科

      Volume: 63 (5) Pages: 833-838

    • NAID

      40022254470

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 腱・靱帯研究のフロンティア(11)「椎間板の形成と恒常性の維持」2020

    • Author(s)
      三浦 重徳、宿南 知佐
    • Journal Title

      整形・災害外科

      Volume: 63 (12) Pages: 1685-1689

    • NAID

      40022415475

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Loss of tenomodulin expression is a risk factor for age-related intervertebral disc degeneration.2020

    • Author(s)
      Lin D, Alberton P, Delgado Caceres M, Prein C, Clausen-Schaumann H, Dong J, Aszodi A, Shukunami C, Iatridis JC, Docheva D.
    • Journal Title

      Aging Cell

      Volume: 19

    • DOI

      10.1111/acel.13091

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Metalloprotease-dependent attenuation of BMP signaling restricts cardiac neural crest cell fate.2020

    • Author(s)
      Arai H, Sato F, Yamamoto T, Woltjen K, Kiyonari H, Yoshimoto Y, Shukunami C, Akiyama H, Kist R, Sehara-Fujisawan A.
    • Journal Title

      Cell Reports

      Volume: 29 Pages: 603-616

    • DOI

      10.1016/j.celrep.2019.09.019

    • NAID

      120006731597

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transient and lineage-restricted requirement of Ebf3 for sternum ossification2020

    • Author(s)
      Kuriki M, Sato F, Arai H, Sogabe M, Kaneko M, Kiyonari H, Kawakami K, Yoshimoto Y, Shukunami C, and Sehara-Fujisawa A.
    • Journal Title

      Development

      Volume: 147 Issue: 9

    • DOI

      10.1242/dev.186239

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 腱・靱帯研究のフロンティア 「腱・靱帯研究のあゆみ」2020

    • Author(s)
      宿南 知佐
    • Journal Title

      整形・災害外科

      Volume: 63 Pages: 83-88

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Scxを中心とした腱・靱帯・歯周靱帯の機能制御2019

    • Author(s)
      吉本 由紀、宿南 知佐
    • Journal Title

      The Bone

      Volume: 33 Pages: 99-104

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation2019

    • Author(s)
      Aki Takimoto, Chikara Kokubu, Hitomi Watanabe, Tetsushi Sakuma, Takashi Yamamoto, Gen Kondoh, Yuji Hiraki, Chisa Shukunami
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 4605-4605

    • DOI

      10.1038/s41598-019-40810-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 1:シングルセル/空間的遺伝子発現解析から明らかになった多様性を持つ心臓内神経堤細胞の領域性と分化運命決定機構2020

    • Author(s)
      岩瀬晃康, 内島泰信, 瀬谷大貴, 来田真友子, 東山大毅, 松居一悠, 田口明糸, 山本尚吾, 福田史朗, 野村征太郎, 興梠貴英, 宿南知佐, 関真秀, 鈴木穣, 和田洋一郎, 油谷浩幸, 栗原由紀子, 宮川-富田幸子, 栗原裕基
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 2:空間的/シングルセル遺伝子発現情報統合解析が示す心臓内神経堤細胞の多様性と領域性2020

    • Author(s)
      岩瀬晃康, 内島泰信, 瀬谷大貴, 来田真友子, 東山大毅, 松居一悠, 田口明糸, 山本尚吾, 福田史朗, 野村征太郎, 興梠貴英, 宿南知佐, 関真秀, 鈴木穣, 和田洋一郎, 油谷浩幸, 栗原由紀子, 宮川-富田幸子, 栗原裕基
    • Organizer
      第19回日本心臓血管発生研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ScxGFP iPS細胞を用いたin vitro腱・靭帯細胞分化誘導システムの確立2020

    • Author(s)
      吉本由紀, 黒澤珠希, 周赫英, 上住円, 外丸祐介, 宿南知佐, 上住聡芳
    • Organizer
      第6回日本筋学学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 1:Establishment of an in vitro culture system for tenogenic/ligamentogenic differentiation using ScxGFP iPS cells2020

    • Author(s)
      宿南 知佐
    • Organizer
      第68回国際歯科研究学会日本部会総会・学術大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 筋骨格システムのコンポーネントにおけるScxとSox9の機能的役割2020

    • Author(s)
      宿南 知佐
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 筋骨格システムを統合する腱の形成と成熟プロセス2019

    • Author(s)
      宿南 知佐
    • Organizer
      日本筋学会 第5回学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ScxGFP iPS細胞を用いた腱・靱帯分化誘導系の構築2019

    • Author(s)
      宿南 知佐
    • Organizer
      第20回運動器科学研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ScxGFP iPS 細胞を用いたin vitro分化誘導系の構築と腱・靭帯・軟骨細胞の分化制御の解析2019

    • Author(s)
      吉本 由紀、藤田 和将、宿南 知佐
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 硬組織連結に寄与するScx+/Sox9+細胞の蛍光イメージング2019

    • Author(s)
      藤田 和将、吉本 由紀、秋山 治彦、今村 健志、宿南 知佐
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ScxGFP iPS細胞の樹立と腱・靭帯分化誘導系の確立2019

    • Author(s)
      吉本 由紀, 藤田 和将, 山家 新勢, 外丸 祐介, 宿南 知佐
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 腱・靱帯研究の現状と展望2018

    • Author(s)
      宿南 知佐
    • Organizer
      日本骨代謝学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ScleraxisとOsterixによる歯周靭帯と歯槽骨のリモデリング制御2018

    • Author(s)
      宿南 知佐
    • Organizer
      歯科基礎医学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 腱・靭帯研究のためのインビボシステムの構築2018

    • Author(s)
      宿南 知佐
    • Organizer
      運動器科学研究会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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