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Understanding of molecular mechanisms of tooth development using iPS interference and application to tooth regeneration techniques

Research Project

Project/Area Number 18H02991
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 57050:Prosthodontics-related
Research InstitutionOkayama University

Principal Investigator

Kuboki Takuo  岡山大学, 医歯薬学総合研究科, 教授 (00225195)

Co-Investigator(Kenkyū-buntansha) 大野 充昭  岡山大学, 医歯薬学総合研究科, 准教授 (60613156)
辻 孝  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (50339131)
渡辺 亮  京都大学, 医学研究科, 特定准教授 (60506765)
宝田 剛志  岡山大学, 医歯薬学総合研究科, 教授 (30377428)
ハラ エミリオ・サトシ  岡山大学, 医歯薬学総合研究科, 研究准教授 (40779443)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords歯の再生 / 転写因子 / iPS干渉 / 象牙芽細胞 / エナメル芽細胞 / iPS干渉法 / 象牙芽細胞マスター遺伝子 / エナメル芽細胞マスター遺伝子 / 器官原基法 / 象牙芽細胞マスター転写因子 / エナメル芽細胞マスター転写因子
Outline of Final Research Achievements

Whole-tooth regeneration is ultimate goal in dental field and the goal of this study is to identify the master transcription factors that characterize tooth germ derived epithelial and mesenchymal cells and develop method to generate those cells. First, we analyzed the mouse embryonic tooth germ and human stem cell from the apical papilla (hSCAP) by RNA-Seq and performed iPS interference to identify the potential transcription factors. Finally, candidate transcription factors were overexpressed in human adult dermal fibroblast (hADF), resulting that hADF was induced into the cells similar to tooth germ derived mesenchymal cells.

Academic Significance and Societal Importance of the Research Achievements

歯の再生医療は歯科疾患に対する究極の治療法として次世代歯科治療に位置づけられる重要な課題である.歯の再生医療の技術開発は,基礎研究としての高い価値を有するだけでなく,臨床応用化に向けたトランスレーショナルな面も持つ研究であり,本研究は歯科の変革に留まらず,上皮間葉相互作用で形成される全身臓器の再生医療の創成という国民の期待に応える国策にも値する重要な意義を有している.

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (9 results)

All 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments2020

    • Author(s)
      Nosho Shuji、Tosa Ikue、Ono Mitsuaki、Hara Emilio Satoshi、Ishibashi Kei、Mikai Akihiro、 Tanaka Yukie、Kimura-Ono Aya、Komori Taishi、Maekawa Kenji、Kuboki Takuo、Oohashi Toshitaka
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 21 Pages: 7967-7967

    • DOI

      10.3390/ijms21217967

    • NAID

      120006937500

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model2020

    • Author(s)
      Mikai Akihiro、Ono Mitsuaki、Tosa Ikue、Nguyen Ha Thi Thu、Hara Emilio Satoshi、Nosho Shuji、Kimura-Ono Aya、Nawachi Kumiko、Takarada Takeshi、Kuboki Takuo、Oohashi Toshitaka
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 19 Pages: 7028-7028

    • DOI

      10.3390/ijms21197028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation.2019

    • Author(s)
      Nomura Y, Hara E.S, Yoshioka Y, Nguyen H.T, Nosho S, Komori T, Ishibashi K, Oohashi T, Ono M, Kuboki T.
    • Journal Title

      Cells Tissues Organs

      Volume: 207 Issue: 3-4 Pages: 115-126

    • DOI

      10.1159/000502885

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone Marrow Cells Inhibit BMP-2-Induced Osteoblast Activity in the Marrow Environment2018

    • Author(s)
      Nguyen Ha Thi、Ono Mitsuaki、Oida Yasutaka、Hara Emilio Satoshi、Komori Taishi、Akiyama Kentaro、Nguyen Ha Thi Thu、Aung Kyaw Thu、Pham Hai Thanh、Tosa Ikue、Takarada Takeshi、Matsuo Koichi、Mizoguchi Toshihide、Oohashi Toshitaka、Kuboki Takuo
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: 34 Issue: 2 Pages: 327-332

    • DOI

      10.1002/jbmr.3598

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A multi-centered epidemiological study evaluating the validity of the treatment difficulty indices developed by the Japan Prosthodontic Society.2018

    • Author(s)
      Kuboki T, Ichikawa T, Baba K, Fujisawa M, Sato H, Aita H, Koyama S, Hideshima M, Sato Y, Wake H, Kimura-Ono A, Nagao K, Kodaira-Ueda Y, Tamaki K, Sadamori S, Tsuga K, Nishi Y, Sawase T, Koshino H, Masumi SI, Sakurai K, Ishibashi K, Ohyama T, Akagawa Y, Hirai T, Sasaki K, Koyano K, Yatani H, Matsumura H
    • Journal Title

      Journal of Prosthodontic Reserach

      Volume: 62(2) Pages: 162-170

    • NAID

      130006733088

    • Related Report
      2018 Annual Research Report
  • [Presentation] 骨髄存在・非存在下にてrhBMP-2,rhFGF-2が骨形成に与える影響の検討2020

    • Author(s)
      納所秋二,大野充昭,土佐郁恵,三海晃弘,石橋 啓,大野 彩,窪木拓男,大橋俊孝
    • Organizer
      公益社団法人日本補綴歯科学会第129回学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] scRNA-Seq解析を応用したマウス歯胚発生メカニズムの解明の試み2020

    • Author(s)
      枝松 緑,小川美帆,井出亮太郎,窪木拓男,大橋俊孝,辻 孝,大野充昭
    • Organizer
      岡山大学次世代研究拠点シンポジウム 2020
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biological Regenerative Medicine in Dental Practice -to Attain Reliable and Sophisticated Dental Therapy-.2018

    • Author(s)
      Kuboki T
    • Organizer
      Special Invited Lecture in ACTA 2018.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Collaboration between Soft Tissue Management and Oral Implant Therapy to Attain Optium Esthetic Result.2018

    • Author(s)
      Kuboki T
    • Organizer
      Special Lecture in Haiphong 2018.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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