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Morphogenesis-based manufacturing: Construction of optimal structures utilizing mechanical adaptation phenomena

Research Project

Project/Area Number 18H03752
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 18:Mechanics of materials, production engineering, design engineering, and related fields
Research InstitutionNagoya University

Principal Investigator

Matsumoto Takeo  名古屋大学, 工学研究科, 教授 (30209639)

Co-Investigator(Kenkyū-buntansha) 前田 英次郎  名古屋大学, 工学研究科, 准教授 (20581614)
村瀬 晃平  名古屋大学, 工学研究科, 准教授 (80298934)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Fiscal Year 2020: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2019: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2018: ¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
Keywordsバイオメカニクス / 力学的適応 / ものづくり / 骨 / 珪藻 / 形態形成 / FRET
Outline of Final Research Achievements

Three themes were studied: In A) Development of a method to clarify the stress distribution in a tissue at the cellular level, we aimed to introduce the FRET-type tension sensor gene into the vascular tissue, but was unsuccessful. We came up with a method to inject sensor cells with stable expression into a tissue as a strain gauge. For B) the establishment of a culture system under the mechanical load of the juvenile bone tissue and the spontaneous creation of the optimum structure, juvenile chick tibial thin sections were cultured while being pulled under a microscope. Calcification was promoted by tension, and moves toward the direction of collagen fibers. In C) the examination of the effect of mechanical stimuli on the morphogenesis of diatoms, it was clarified that the intracellular pressure may be >200 kPa from the measurement of the elongation force during growth of Aulacoseira and the relationship between the osmotic pressure of the culture medium and the growth rate.

Academic Significance and Societal Importance of the Research Achievements

生物のかたちづくりには力が大きく関わっており,生体組織の多くが力学的最適性を保っている.そこで「生物のかたちづくりに力学はどのように影響しているのか,それを上手く制御して,ものづくりに結びつけることはできないか?」という考えで研究を進めた.組織内張力分布を細胞レベルで明らかにする系の確立には失敗したが,将来有望な方法を着想した.幼若骨を力学負荷下で培養し,最適構造を自発創生させる手法の確立に挑み,基礎データを蓄積した.同じく珪藻を力学負荷下で培養し,被殻形成過程の詳細や細胞内圧など,基礎データの蓄積を行った.

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (33 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 3 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Brno University of Technology(チェコ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Through the cleared aorta: three-dimensional characterization of mechanical behaviors of rat thoracic aorta under intraluminal pressurization using optical clearing method2022

    • Author(s)
      Maeda E, Ando Y, Takeshita K, Matsumoto T
    • Journal Title

      Scientific Reports

      Volume: In press

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microscopic characterisation of local strain field in healing tissue in the central third defect of mouse patellar tendon at early-phase of healing2021

    • Author(s)
      Maeda Eijiro、Kuroyanagi Kaname、Matsumoto Takeo
    • Journal Title

      Journal of the Mechanical Behavior of Biomedical Materials

      Volume: 123 Pages: 104702-104702

    • DOI

      10.1016/j.jmbbm.2021.104702

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞核への力学刺激と核内クロマチン分布2020

    • Author(s)
      松本健郎
    • Journal Title

      医学のあゆみ『ラミノパチーとはなにか』

      Volume: 272-2 Pages: 147-151

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Journal Article] 力学現象としての動脈硬化2020

    • Author(s)
      松本健郎
    • Journal Title

      実験医学 2020年5月増刊号『疾患に挑むメカノバイオロジー』

      Volume: 38-7 Pages: 30-35

    • Related Report
      2020 Annual Research Report
  • [Journal Article] A novel FRET analysis method for tension dynamics in a single actin stress fiber: Application to MC3T3-E1 cells during movement on a substrate2019

    • Author(s)
      Wang Junfeng、Sugita Shukei、Michiue Tatsuo、Tsuboi Takashi、Kitaguchi Tetsuya、Matsumoto Takeo
    • Journal Title

      Journal of Biorheology

      Volume: 33 Issue: 1 Pages: 21-26

    • DOI

      10.17106/jbr.33.21

    • NAID

      130007674886

    • ISSN
      1867-0466, 1867-0474
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch2019

    • Author(s)
      Maeda Eijiro、Atsumi Yoshinori、Ishiguro Mai、Nagayama Kazuaki、Matsumoto Takeo
    • Journal Title

      Journal of Biomechanics

      Volume: 96 Pages: 109371-109371

    • DOI

      10.1016/j.jbiomech.2019.109371

    • NAID

      120006808764

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoelasticity-based evaluation of cellular contractile force for phenotypic discrimination of vascular smooth muscle cells2019

    • Author(s)
      Sugita Shukei, Mizutani Eri, Hozaki Masatoshi, Nakamura Masanori, Matsumoto Takeo
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 3960-3960

    • DOI

      10.1038/s41598-019-40578-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Finite Element Bendo-Tensegrity Model of Eukaryotic Cell2018

    • Author(s)
      Bansod Yogesh Deepak、Matsumoto Takeo、Nagayama Kazuaki、Bursa Jiri
    • Journal Title

      Journal of Biomechanical Engineering

      Volume: 140 Issue: 10 Pages: 101001-101001

    • DOI

      10.1115/1.4040246

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ex-vivo observation of calcification process in chick tibia slice: Augmented calcification along collagen fiber orientation in specimens subjected to static stretch2018

    • Author(s)
      Maeda E, Ichikawa K, Murase K, Nagayama K, Matsumoto T
    • Journal Title

      J Biomechanics

      Volume: 78 Pages: 94-101

    • DOI

      10.1016/j.jbiomech.2018.07.024

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct application of mechanical stimulation to cell adhesion sites using a novel magnetic-driven micropillar substrate2018

    • Author(s)
      Nagayama K, Inoue T, Hamada Y, Sugita S, Matsumoto T
    • Journal Title

      Biomed Microdevices

      Volume: 20 Issue: 4 Pages: 85-85

    • DOI

      10.1007/s10544-018-0328-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 血管壁に導入したFRET型張力センサ発現平滑筋細胞の形態変化観察2022

    • Author(s)
      道家大悟,沼野翔太,王軍鋒,キム・ジョンヒョン,前田英次郎,松本健郎
    • Organizer
      日本機械学会第32回バイオフロンティア講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] FRET型張力センサを発現するトランスジェニックマウスの作製と評価2021

    • Author(s)
      王軍鋒,辻村有紀,北口哲也,前田英次郎,横田秀夫,松本健郎
    • Organizer
      日本機械学会2021年度年次大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Generation and evaluation of transgenic mice expressing tension sensor based on FRET2021

    • Author(s)
      Wang JF, Tsujimura Y, Kitaguchi T, Maeda E, Yokota H, Matsumoto T
    • Organizer
      The 11th Asian-Pacific Conference on Biomechanics
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 血管壁の3次元微細構造を明らかにする画像化技術2020

    • Author(s)
      松本健郎,横田秀夫,杉田修啓,安東頼子
    • Organizer
      第59回日本生体医工学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 弾性動脈のLamellar Unitと力学応答:高血圧に対する胸大動脈壁の力学的適応の解明を目指して2020

    • Author(s)
      松本健郎
    • Organizer
      Researcher Conference for Pulmonary Circulation & Right Heart Function (Re-PCR 2020)
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] FRET型張力センサを用いた単一ストレスファイバ内の微視的変形計測:変形の不均一性およびFRET ratio 変化と実際のひずみとの相関の検討2020

    • Author(s)
      小出恭平,王軍鋒,前田英次郎,松本健郎
    • Organizer
      日本機械学会第31回バイオフロンティア講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生体軟組織へのFRETセンサ導入法の開発:組織内微視的ひずみ分布の計測を目指して2020

    • Author(s)
      沼野翔太,王軍鋒,前田英次郎,松本健郎
    • Organizer
      日本機械学会第31回バイオフロンティア講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 鶏雛幼若骨組織の石灰化に及ぼすコラーゲン線維の影響の評価:骨の力学的適応メカニズムの解明を目指して2020

    • Author(s)
      茅根義明,前田英次郎,松本健郎
    • Organizer
      第29回ライフサポート学会フロンティア講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 動脈壁の力学応答解明を目指したマルチスケール・バイオメカニクス2019

    • Author(s)
      松本健郎
    • Organizer
      日本機械学会中四国支部・特別講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 鶏雛幼若骨組織薄切片の石灰化の力学応答観察:骨の力学的適応を用いた形態形成誘導法の確立を目指して2019

    • Author(s)
      茅根義明,前田英次郎,松本健郎
    • Organizer
      日本機械学会2019年度年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 培養鶏雛幼若骨組織薄切片の石灰化プロセスに及ぼすコラーゲン線維配向とひずみ分布の影響2019

    • Author(s)
      茅根義明,前田英次郎,松本健郎
    • Organizer
      日本機械学会第32回バイオエンジニアリング講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 珪藻の力学負荷下の被殻形成に関する基礎的研究2019

    • Author(s)
      細川拓都,前田英次郎,松本健郎
    • Organizer
      日本機械学会第32回バイオエンジニアリング講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Changes in FRET ratio distribution along single isolated stress fibers expressing FRET-based actinin tension sensor during stretch2018

    • Author(s)
      Matsumoto T, Ito M, Maeda E, Wang JF, Matsui T, Deguchi S, Sugita S
    • Organizer
      2018 Biomedical Engineering Society Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FRETイメージングを用いたMC3T3-E1細胞内張力のダイナミクスの計測2018

    • Author(s)
      王 軍鋒,杉田修啓,道上達男,坪井貴司,北口哲也,松本健郎
    • Organizer
      第41回日本バイオレオロジー学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] すり鉢様形状の培養基板面上で培養された細胞が示す応答2018

    • Author(s)
      金森宗一郎,前田英次郎,村瀬晃平,松本健郎
    • Organizer
      第57回日本生体医工学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] FRET張力センサを導入した単離ストレスファイバの引張試験2018

    • Author(s)
      王 軍鋒,伊藤将大,松井 翼,出口真次,前田英次郎,松本健郎
    • Organizer
      日本機械学会2018年度年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 微細溝構造に沿った細胞の配向現象に基板面の巨視的な曲率が与える影響2018

    • Author(s)
      金森宗一郎,前田英次郎,村瀬晃平,松本健郎
    • Organizer
      日本機械学会第31回バイオエンジニアリング講演会
    • Related Report
      2018 Annual Research Report
  • [Book] 最先端ナノライフシステム研究(最先端ナノライフシステム研究編集委員会編)2022

    • Author(s)
      キム・ジョンヒョン,前田英次郎,松本健郎
    • Total Pages
      221
    • Publisher
      丸善プラネット
    • ISBN
      9784863455207
    • Related Report
      2020 Annual Research Report
  • [Book] Biomechanics of Coronary Atherosclerotic Plaque: From Model to Patient (Ohayon J, Finet G, Pettigrew RI, eds.)2020

    • Author(s)
      Jean-Louis Martiel, Gerard Finet, Gerhard Holzapfel, Matthias Stuber, Takeo Matsumoto, Roderic I. Pettigrew, Jacques Ohayon
    • Total Pages
      675
    • Publisher
      Academic Press
    • ISBN
      9780323859332
    • Related Report
      2020 Annual Research Report
  • [Remarks] バイオメカニクス研究室

    • URL

      http://bio.mech.nagoya-u.ac.jp/

    • Related Report
      2020 Annual Research Report 2018 Annual Research Report
  • [Remarks] 名古屋大学・バイオメカニクス研究室

    • URL

      http://bio.mech.nagoya-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体情報測定装置2018

    • Inventor(s)
      松本健郎,益田博之
    • Industrial Property Rights Holder
      松本健郎,益田博之
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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