| Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2020: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2019: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2018: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
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| Outline of Final Research Achievements |
In this study, we developed chemical protein labeling probes and applied them for analyzing the dynamics of membrane proteins in living cells. First, we applied a PYP tag labeling method to investigate why glucose transporter GLUT4 is retained in the plasma membrane by glycosylation. Second, we developed near-infrared and red fluorescent probes based on BL-tag system for imaging membrane proteins and analyzing the dynamics in response to ligand stimulation. In addition, we developed an improved labeling system using new ligand structures and beta-lactamase. Using these chemical probes, we provided a method for analyzing membrane protein dynamics and protein-protein interactions.
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