|Budget Amount *help
¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000)
Fiscal Year 2021: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2020: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
|Outline of Final Research Achievements
In this study, the molecular mechanism of polyU addition to pre-let7 miRNA by TUT4/7, in the presence of Lin28 protein, was clarified by structural and biochemical analysis of the Lin28-interacting region of TUT4/7. The molecular mechanism of polyU addition to the 3' -end of U6 snRNA by TUT1 was also clarified by structural analyses of TUT1 in complex with U6 snRNA, together with the biochemical analysis. We also successfully determined the structure of core region of TENT4/4B, which adds an A/G mix tail to the polyA tail of RNA, providing insight into nucleotide specificity. We also succeeded in determining the structures of various RNA modification enzymes and processing enzymes and clarified the molecular mechanisms of substrate specificity and regulation of their chemical reactions.