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Molecular developmental basis of the asexual reproductive mode "stolonization" in syllid annelids

Research Project

Project/Area Number 18H04006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Miura Toru  東京大学, 大学院理学系研究科(理学部), 教授 (00332594)

Co-Investigator(Kenkyū-buntansha) 越川 滋行  北海道大学, 地球環境科学研究院, 教授 (30714498)
林 良信  慶應義塾大学, 法学部(日吉), 講師 (70626803)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2022: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2018: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Keywordsストロナイゼーション / 無性生殖 / 生活史 / 頭部形成 / 性決定 / 環形動物 / シリス / 放精放卵 / 神経系 / 表現型可塑性 / 繁殖様式 / ボディプラン / パターン形成遺伝子 / 放精抱卵
Outline of Final Research Achievements

In species belonging to the family Syllidae in the phyum Annelida, mature individuals develop gonad in the posterior part of their body, where an ectopic head was newly formed. Then, the posterior body part filled with gonad detaches from the original body to swim and spawn eggs or sperms for sexual reproduction. This unique process of reproduction is called "stolonization", but the underlying developmental mechanism has yet to be elucidated. Therefore, in this research project, focusing on a Japanese green syllid Megasyllis nipponica, the rearing system was firstly established in the laboratory. Then, the detailed process of stolonization was investigated by using multiple analytical approaches, for example, by scanning electoron microscopy and confocal microscope. Based on the observation results, gene expression analyses were also carried out, revealing that some of the patterning genes are recruited for the formation of an ectopic heads.

Academic Significance and Societal Importance of the Research Achievements

多細胞動物の発生学は研究の歴史が深いが、その大部分はモデル実験動物における胚発生の研究に終始しており、野外に生息する非モデル生物の、特に後胚発生の様式については、ほとんど解明されていないと言っても過言ではない。そのような生物の中には、現在の発生学の常識からは逸脱したものも多数存在しており、その中のひとつが、シリスの仲間のストロナイゼーションという繁殖様式だろう。後胚発生の過程で、身体の途中にもうひとつの頭部が形成されるという特異な発生過程の根底には、これまでに知られる発生過程とは異なる何かが存在するはずである。本研究課題の遂行によって、その一端が明らかとなり、生物学に新たな地平をもたらした。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (61 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (10 results) Journal Article (27 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 25 results,  Open Access: 10 results) Presentation (15 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results) Remarks (9 results)

  • [Int'l Joint Research] Georg-August-Universitat Gettingen(ドイツ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Universidad Autonoma de Madrid(スペイン)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Georg-August-Universitat Gettingen(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Universidad Autonoma de Madrid(スペイン)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Universidad Autonoma de Madrid(スペイン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Georg-August-Universitat Gottingen(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Universidad Autonoma de Madrid(スペイン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Georg-August-Universitat Gottingen(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Universidad Autonoma de Madrid(スペイン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Georg-August-Universitat Gottingen(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Appearance of a transparent protrusion containing two pairs of legs on the apodous ring preceding the anamorphic molt in a millipede, Niponia nodulosa2023

    • Author(s)
      Chiyoda Soma、Oguchi Kohei、Miura Toru
    • Journal Title

      Frontiers in Zoology

      Volume: 20 Issue: 1 Pages: 14-14

    • DOI

      10.1186/s12983-023-00493-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction of a massive genetic resource by transcriptome sequencing and genetic characterization of <i>Megasyllis nipponica</i> (Annelida: Syllidae)2022

    • Author(s)
      Hayashi Yoshinobu、Oguchi Kohei、Nakamura Mayuko、Koshikawa Shigeyuki、Miura Toru
    • Journal Title

      Genes & Genetic Systems

      Volume: 97 Issue: 3 Pages: 153-166

    • DOI

      10.1266/ggs.21-00137

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2022-06-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The pentameric hydrocoel lobes organize adult pentameral structures in a sea cucumber, Apostichopus japonicus2022

    • Author(s)
      Udagawa Sumio、Nagai Akiko、Kikuchi Mani、Omori Akihito、Tajika Atsushi、Saito Mieko、Miura Toru、Irie Naoki、Kamei Yasuhiro、Kondo Mariko
    • Journal Title

      Developmental Biology

      Volume: 492 Pages: 71-78

    • DOI

      10.1016/j.ydbio.2022.09.002

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pleopodal lung development in a terrestrial isopod, Porcellio scaber (Oniscidea)2022

    • Author(s)
      Inui Naoto、Kimbara Ryosuke、Yamaguchi Haruka、Miura Toru
    • Journal Title

      Arthropod Structure & Development

      Volume: 71 Pages: 101210-101210

    • DOI

      10.1016/j.asd.2022.101210

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptomics on Social Interactions in Termites: Effects of Soldier Presence2022

    • Author(s)
      Matsunami Masatoshi、Watanabe Dai、Fujiwara Kokuto、Hayashi Yoshinobu、Shigenobu Shuji、Miura Toru、Maekawa Kiyoto
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 10 Pages: 924151-924151

    • DOI

      10.3389/fevo.2022.924151

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ontogenetic Expressions of Sexually Dimorphic Traits in the Skeleton Shrimp Caprella scaura (Crustacea: Amphipoda)2022

    • Author(s)
      Otomo Yohei、Shinji Junpei、Kohtsuka Hisanori、Miura Toru
    • Journal Title

      Zoological Science

      Volume: 39 Issue: 5 Pages: 431-445

    • DOI

      10.2108/zs220038

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hormone-related genes heterochronically and modularly regulate neotenic differentiation in termites2022

    • Author(s)
      Oguchi Kohei、Koshikawa Shigeyuki、Miura Toru
    • Journal Title

      Developmental Biology

      Volume: 485 Pages: 70-79

    • DOI

      10.1016/j.ydbio.2022.02.012

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Understanding of superorganisms: collective behavior, differentiation and social organization2022

    • Author(s)
      Miura Toru、Oguchi Kohei、Yamaguchi Haruka、Nakamura Mayuko、Sato Daisuke、Kobayashi Kenta、Kutsukake Nobuyuki、Miura Kyoko、Hayashi Yoshinobu、Hojo Masaru、Maekawa Kiyoto、Shigenobu Shuji、Kano Takeshi、Ishiguro Akio
    • Journal Title

      Artificial Life and Robotics

      Volume: 27 Issue: 2 Pages: 204-212

    • DOI

      10.1007/s10015-022-00754-x

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Four new species of <i>Ctenodrilus</i>, <i>Raphidrilus</i>, and <i>Raricirrus</i> (Cirratuliformia, Annelida) in Japanese waters, with notes on their phylogenetic position2022

    • Author(s)
      Jimi Naoto、Fujimoto Shinta、Fujiwara Yoshihiro、Oguchi Kohei、Miura Toru
    • Journal Title

      PeerJ

      Volume: 10 Pages: e13044-e13044

    • DOI

      10.7717/peerj.13044

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ramisyllis kingghidorahi n. sp., a new branching annelid from Japan2022

    • Author(s)
      Aguado M. Teresa、Ponz-Segrelles Guillermo、Glasby Christopher J.、Ribeiro Rannyele P.、Nakamura Mayuko、Oguchi Kohei、Omori Akihito、Kohtsuka Hisanori、Fischer Christian、Ise Yuji、Jimi Naoto、Miura Toru
    • Journal Title

      Organisms Diversity &amp; Evolution

      Volume: 22 Issue: 2 Pages: 377-405

    • DOI

      10.1007/s13127-021-00538-4

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Parasitism-Induced Intersexuality in a Sexually Dimorphic Varunid Crab, Ptychognathus ishii (Decapoda: Varunidae)2021

    • Author(s)
      Inui Naoto、Oguchi Kohei、Shinji Junpei、Okanishi Masanori、Shimomura Michitaka、Miura Toru
    • Journal Title

      Zoological Science

      Volume: 38 Issue: 5 Pages: 416-426

    • DOI

      10.2108/zs210049

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulatory Mechanisms Underlying the Differentiation of Neotenic Reproductives in Termites: Partial Release From Arrested Development2021

    • Author(s)
      Oguchi Kohei、Maekawa Kiyoto、Miura Toru
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 9 Pages: 635552-635552

    • DOI

      10.3389/fevo.2021.635552

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Reproductive workers insufficiently signal their reproductive ability in a paper wasp.2020

    • Author(s)
      Tsuchida, K., Saigo, T., Asai, K., Okamoto, T., Ando, M., Ando, T., Sasaki, K., Yokoi, K., Watanabe, D., Sugime, Y., Miura, T.
    • Journal Title

      Behavioral Ecology

      Volume: 31 Issue: 2 Pages: 577-590

    • DOI

      10.1093/beheco/arz212

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Meiobenthic polychaete Dinophilus sp. cf. gyrociliatus (Annelida: Dinophilidae) from Japan with SEM observation and DNA barcodes.2020

    • Author(s)
      Jimi N, Minokawa T, Miura T, Kajihara H
    • Journal Title

      Species Diversity

      Volume: 251 Issue: 2 Pages: 213-218

    • DOI

      10.12782/specdiv.25.213

    • NAID

      130007905683

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The making of the defensive caste: Physiology, development and evolution of the soldier differentiation in termites.2020

    • Author(s)
      Miura T & Maekawa K
    • Journal Title

      Evolution & Development

      Volume: 22 Issue: 6 Pages: 425-437

    • DOI

      10.1111/ede.12335

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Male neotenic reproductives accelerate additional differentiation of female reproductives by lowering JH titer in termites.2020

    • Author(s)
      Oguchi K, Sugime Y, Shimoji H, Hayashi Y, Miura T
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 9435-9435

    • DOI

      10.1038/s41598-020-66403-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environment-sensitive intelligenet self-reproducing artificial cell with a modification-active lipo-deoxyribozyme.2020

    • Author(s)
      Matsuo M, Hirata Y, Kurihara K, Toyota T, Miura T, Suzuki K, Sugawara T
    • Journal Title

      Micromachines

      Volume: 11 Issue: 6 Pages: 606-606

    • DOI

      10.3390/mi11060606

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of genes involved in offensive and defensive phenotype induction in the pituitary gland of the Hokkaido salamander (Hynobius retardatus).2020

    • Author(s)
      Matsunami M, Miura T, Kishida O, Michimae H, Nishimura K
    • Journal Title

      Zoological Science

      Volume: 37 Issue: 6 Pages: 563-574

    • DOI

      10.2108/zs190140

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pattern of sucker development in cuttlefishes.2020

    • Author(s)
      Kimbara R, Nakamura M, Oguchi K, Kohtsuka H, Miura T
    • Journal Title

      Frontiers in Zoology

      Volume: 17 Issue: 1 Pages: 24-24

    • DOI

      10.1186/s12983-020-00371-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Life cycle of the Japanese green syllid, Megasyllis nipponica (Annelida: Syllidae): Field collection and establishment of rearing system.2019

    • Author(s)
      Miura T, Oguchi K, Nakamura M, Jimi N, Miura S, Hayashi Y, Koshikawa S, Aguado MT
    • Journal Title

      Zoological Science

      Volume: 36 Issue: 5 Pages: 372-379

    • DOI

      10.2108/zs190058

    • NAID

      210000171134

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Unique morphogenesis in the damp-wood termite: abscission of the stylus during female reproductive caste differentiation.2019

    • Author(s)
      Oguchi K, Miura T
    • Journal Title

      Zoological Science

      Volume: 36 Issue: 5 Pages: 380-386

    • DOI

      10.2108/zs190056

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental process of cerata in the cladobranchian sea slug Pteraeolidia semperi (Mollusca: Gastropoda: Nudibranchia).2019

    • Author(s)
      Togawa Y, Shinji J, Fukatsu T, Miura T
    • Journal Title

      Zoological Science

      Volume: 36 Issue: 5 Pages: 387-394

    • DOI

      10.2108/zs190057

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Duplication and soldier-specific expression of geranylgeranyl diphosphate synthase genes in a nasute termite Nasutitermes takasagoensis2019

    • Author(s)
      Hojo Masaru、Shigenobu Shuji、Maekawa Kiyoto、Miura Toru、Tokuda Gaku
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 111 Pages: 103177-103177

    • DOI

      10.1016/j.ibmb.2019.103177

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Termite soldier mandibles are elongated by dachshund under hormonal and Hox gene controls2019

    • Author(s)
      Sugime Y, Oguchi K, Gotoh H, Hayashi Y, Matsunami M, Shigenobu S, Koshikawa S, Miura T
    • Journal Title

      Development

      Volume: 146 Issue: 5 Pages: 1-9

    • DOI

      10.1242/dev.171942

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reduction of a nymphal instar in a dampwood termite: heterochronic shift in the caste differentiation pathways.2019

    • Author(s)
      Nii R, Oguchi K, Shinji J, Koshikawa S, Miura T
    • Journal Title

      EvoDevo

      Volume: 10 Issue: 1 Pages: 10-10

    • DOI

      10.1186/s13227-019-0123-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Juvenile hormone as a physiological regulator mediating phenotypic plasticity in pancrustaceans2018

    • Author(s)
      Miura T
    • Journal Title

      Development, Growth & Differentiation

      Volume: 61 Issue: 1 Pages: 85-96

    • DOI

      10.1111/dgd.12572

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pheromone gland development and monoterpenoid synthesis specific to oviparous females in the pea aphid.2018

    • Author(s)
      Murano K, Ogawa K, Kaji T, Miura T
    • Journal Title

      Zool Lett

      Volume: 4 Issue: 1 Pages: 9-9

    • DOI

      10.1186/s40851-018-0092-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 環形動物ミドリシリスのストロナイゼーションにおける特異な尾部形成2023

    • Author(s)
      佐藤大介, 中村真悠子, 三浦徹.
    • Organizer
      2023年日本動物学会 第75回 関東支部大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] トゲワレカラ(軟甲綱端脚目)の後胚発生における性的二型の発現過程2022

    • Author(s)
      大友洋平, 進士淳平, 幸塚久典, 三浦徹
    • Organizer
      第74回日本動物学会関東支部大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 環形動物ミドリシリスの有性生殖に伴う第2の尾の形成過程2021

    • Author(s)
      佐藤大介,中村真悠子,三浦徹
    • Organizer
      日本ベントス学会・日本プランクトン学会合同大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イカ類における吸盤形成関連遺伝子の探索2021

    • Author(s)
      金原僚亮,幸塚久典,三浦徹
    • Organizer
      第四回イカタコ研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Understanding of superorganisms: collective behavior, differentiation and social organization2021

    • Author(s)
      Toru Miura
    • Organizer
      DARS/SWARM 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 様々な動物系統における表現型可塑性と生活史の進化2020

    • Author(s)
      三浦徹
    • Organizer
      生物情報若手の会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 研究分野の壁を越えて:社会生物学から生態発生学、そして進化動物学へ2019

    • Author(s)
      三浦徹
    • Organizer
      第44回日本比較内分泌学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ミサキムチョウウズムシの繁殖様式と生殖腺の発達2019

    • Author(s)
      三浦徹
    • Organizer
      日本動物学会 第90回大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 動物生活史におけるボディプランの変更ー「個体性」を超えてー2019

    • Author(s)
      三浦徹
    • Organizer
      日本動物学会 第90回大阪大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 動物進化におけるメジャートランジション2019

    • Author(s)
      三浦徹
    • Organizer
      日本動物学会 第90回大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミドリシリスにおけるストロン形成過程:組織形態学的観察に基づくステージング2019

    • Author(s)
      中村真悠子・小口晃平・三浦徹
    • Organizer
      日本動物学会関東支部第71回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 東大三崎臨海実験所における進化研究の試み2018

    • Author(s)
      三浦徹
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 発生拘束からの逸脱による大規模進化への鍵2018

    • Author(s)
      三浦徹
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ミドリシリスにおけるストロン形成の組織形態学的観察2018

    • Author(s)
      中村真悠子、小口晃平、三浦徹
    • Organizer
      日本動物学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The making of the strongest: Developmental underpinnings of soldier differentiation in termites.2018

    • Author(s)
      Miura, T.
    • Organizer
      International Union for the Study of Social Insects (IUSSI2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] Miura Lab

    • URL

      https://sites.google.com/view/miura-lab/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 東京大学大学院理学系研究科・附属臨海実験所

    • URL

      https://www.mmbs.s.u-tokyo.ac.jp/wp/

    • Related Report
      2022 Annual Research Report
  • [Remarks] Miura Lab (三崎臨海実験所、旧ページ)

    • URL

      https://www.mmbs.s.u-tokyo.ac.jp/research/MiuraLab/index.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] Miura Lab (三崎臨海実験所、新ページ)

    • URL

      https://sites.google.com/view/miura-lab/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 三浦研究室HP

    • URL

      http://www.mmbs.s.u-tokyo.ac.jp/research/MiuraLab/index.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] シロアリにおける生殖虫分化:異性による生理調節機構の解明

    • URL

      https://www.s.u-tokyo.ac.jp/ja/press/2020/6903/

    • Related Report
      2020 Annual Research Report
  • [Remarks] コウイカ類における吸盤形成パターンの解明

    • URL

      https://www.s.u-tokyo.ac.jp/ja/press/2020/6997/

    • Related Report
      2020 Annual Research Report
  • [Remarks] A day in the life of a termite lab

    • URL

      https://thenode.biologists.com/a-day-in-the-life-of-a-termite-lab/lablife/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 東京大学・大学院理学系研究科・附属臨海実験所 三浦研HP

    • URL

      http://www.mmbs.s.u-tokyo.ac.jp/research/MiuraLab/index.html

    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2024-12-25  

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